Genome analysis shows Bacillus axarquiensis is not a later heterotypic synonym of Bacillus mojavensis; reclassification of Bacillus malacitensis and Brevibacterium halotolerans as heterotypic synonyms of Bacillus axarquiensis
Bacillus axarquiensis and Bacillus malacitensis were previously reported to be later heterotypic synonyms of Bacillus mojavensis, based primarily on DNA-DNA relatedness values. We have sequenced draft genomes of Bacillus axarquiensis NRRL B-41617T and Bacillus malacitensis NRRL B-41618T. Comparative...
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| Veröffentlicht in: | International journal of systematic and evolutionary microbiology 2016-06, Vol.66 (6), p.2438-2443 |
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| creator | Dunlap, Christopher A Bowman, Michael J Schisler, David A Rooney, Alejandro P |
| description | Bacillus axarquiensis and Bacillus malacitensis were previously reported to be later heterotypic synonyms of Bacillus mojavensis, based primarily on DNA-DNA relatedness values. We have sequenced draft genomes of Bacillus axarquiensis NRRL B-41617T and Bacillus malacitensis NRRL B-41618T. Comparative genomics and DNA-DNA relatedness calculations showed that while Bacillus axarquiensis and Bacillus malacitensis are synonymous with each other, they are not synonymous with Bacillus mojavensis. In addition, a draft genome was completed for Brevibacterium halotolerans, a strain long suspected of being a Bacillus subtilis group member based on 16S rRNA similarities (99.8 % with Bacillus mojavensis). Comparative genomics and DNA-DNA relatedness calculations showed that Brevibacterium halotolerans is synonymous with Bacillus axarquiensis and Bacillus malacitensis. The pairwise in silico DNA-DNA hybridization values calculated in comparisons between the three conspecific strains were all greater than 92 %, which is well above the standard species threshold of 70 %. While the pairwise in silico DNA-DNA hybridization values calculated in comparisons of the three conspecific strains with Bacillus mojavensis were all less than 65 %. The combined results of our genotype and phenotype studies showed that Bacillus axarquiensis, Bacillus malacitensis and Brevibacterium halotolerans are conspecific and distinct from Bacillus mojavensis. Because the valid publication of the name Bacillus axarquiensis predates the publication of the name Bacillus malacitensis, we propose that Bacillus malacitensis be reclassified as a synonym of Bacillus axarquiensis. In addition, we propose to reclassify Brevibacterium halotolerans as a synonym of Bacillus axarquiensis. An amended description of Bacillus axarquiensis is provided. |
| doi_str_mv | 10.1099/ijsem.0.001048 |
| format | Article |
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We have sequenced draft genomes of Bacillus axarquiensis NRRL B-41617T and Bacillus malacitensis NRRL B-41618T. Comparative genomics and DNA-DNA relatedness calculations showed that while Bacillus axarquiensis and Bacillus malacitensis are synonymous with each other, they are not synonymous with Bacillus mojavensis. In addition, a draft genome was completed for Brevibacterium halotolerans, a strain long suspected of being a Bacillus subtilis group member based on 16S rRNA similarities (99.8 % with Bacillus mojavensis). Comparative genomics and DNA-DNA relatedness calculations showed that Brevibacterium halotolerans is synonymous with Bacillus axarquiensis and Bacillus malacitensis. The pairwise in silico DNA-DNA hybridization values calculated in comparisons between the three conspecific strains were all greater than 92 %, which is well above the standard species threshold of 70 %. While the pairwise in silico DNA-DNA hybridization values calculated in comparisons of the three conspecific strains with Bacillus mojavensis were all less than 65 %. The combined results of our genotype and phenotype studies showed that Bacillus axarquiensis, Bacillus malacitensis and Brevibacterium halotolerans are conspecific and distinct from Bacillus mojavensis. Because the valid publication of the name Bacillus axarquiensis predates the publication of the name Bacillus malacitensis, we propose that Bacillus malacitensis be reclassified as a synonym of Bacillus axarquiensis. In addition, we propose to reclassify Brevibacterium halotolerans as a synonym of Bacillus axarquiensis. An amended description of Bacillus axarquiensis is provided.</description><identifier>ISSN: 1466-5026</identifier><identifier>EISSN: 1466-5034</identifier><identifier>DOI: 10.1099/ijsem.0.001048</identifier><identifier>PMID: 27030978</identifier><language>eng</language><publisher>England</publisher><subject>Bacillus - classification ; Bacterial Typing Techniques ; Base Composition ; Brevibacterium - genetics ; DNA, Bacterial - genetics ; Genome, Bacterial ; Nucleic Acid Hybridization ; Phylogeny ; RNA, Ribosomal, 16S - genetics ; Sequence Analysis, DNA</subject><ispartof>International journal of systematic and evolutionary microbiology, 2016-06, Vol.66 (6), p.2438-2443</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c335t-77dfe337bf6b1ab59c353ffe5648f7599ec64c68503608310158c671ded29c893</citedby><cites>FETCH-LOGICAL-c335t-77dfe337bf6b1ab59c353ffe5648f7599ec64c68503608310158c671ded29c893</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,3733,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27030978$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Dunlap, Christopher A</creatorcontrib><creatorcontrib>Bowman, Michael J</creatorcontrib><creatorcontrib>Schisler, David A</creatorcontrib><creatorcontrib>Rooney, Alejandro P</creatorcontrib><title>Genome analysis shows Bacillus axarquiensis is not a later heterotypic synonym of Bacillus mojavensis; reclassification of Bacillus malacitensis and Brevibacterium halotolerans as heterotypic synonyms of Bacillus axarquiensis</title><title>International journal of systematic and evolutionary microbiology</title><addtitle>Int J Syst Evol Microbiol</addtitle><description>Bacillus axarquiensis and Bacillus malacitensis were previously reported to be later heterotypic synonyms of Bacillus mojavensis, based primarily on DNA-DNA relatedness values. We have sequenced draft genomes of Bacillus axarquiensis NRRL B-41617T and Bacillus malacitensis NRRL B-41618T. Comparative genomics and DNA-DNA relatedness calculations showed that while Bacillus axarquiensis and Bacillus malacitensis are synonymous with each other, they are not synonymous with Bacillus mojavensis. In addition, a draft genome was completed for Brevibacterium halotolerans, a strain long suspected of being a Bacillus subtilis group member based on 16S rRNA similarities (99.8 % with Bacillus mojavensis). Comparative genomics and DNA-DNA relatedness calculations showed that Brevibacterium halotolerans is synonymous with Bacillus axarquiensis and Bacillus malacitensis. The pairwise in silico DNA-DNA hybridization values calculated in comparisons between the three conspecific strains were all greater than 92 %, which is well above the standard species threshold of 70 %. While the pairwise in silico DNA-DNA hybridization values calculated in comparisons of the three conspecific strains with Bacillus mojavensis were all less than 65 %. The combined results of our genotype and phenotype studies showed that Bacillus axarquiensis, Bacillus malacitensis and Brevibacterium halotolerans are conspecific and distinct from Bacillus mojavensis. Because the valid publication of the name Bacillus axarquiensis predates the publication of the name Bacillus malacitensis, we propose that Bacillus malacitensis be reclassified as a synonym of Bacillus axarquiensis. In addition, we propose to reclassify Brevibacterium halotolerans as a synonym of Bacillus axarquiensis. An amended description of Bacillus axarquiensis is provided.</description><subject>Bacillus - classification</subject><subject>Bacterial Typing Techniques</subject><subject>Base Composition</subject><subject>Brevibacterium - genetics</subject><subject>DNA, Bacterial - genetics</subject><subject>Genome, Bacterial</subject><subject>Nucleic Acid Hybridization</subject><subject>Phylogeny</subject><subject>RNA, Ribosomal, 16S - genetics</subject><subject>Sequence Analysis, DNA</subject><issn>1466-5026</issn><issn>1466-5034</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkUFv1DAQhS1ERUvhyhH5yGUXe504jjjRqhSkSlzKOZo4Y61Xjr31JIX8XP5J3W4pVEKy7JHme29Gfoy9k2ItRdt-9DvCcS3WQkhRmRfsRFZar2qhqpdP9UYfs9dEu8JUWohX7HjTCCXaxpyw35cY04gcIoSFPHHapp_Ez8D6EGbi8Avyzewx3vfKiWniwANMmPkWy52mZe8tpyWmuIw8ub_aMe3g9kH5iWe0AYi88xYmn-JzEEIpp8MQiAM_y3jre7DF388j30JIUwqYIZY-_W8wPTP8d-s37MhBIHz7-J6yH18urs-_rq6-X347_3y1skrV06ppBodKNb3TvYS-bq2qlXNY68q4pm5btLqy2pSv1cIoKWRtrG7kgMOmtaZVp-zDwXef082MNHWjJ4shQMQ0UyeNqCuj201V0PUBtTkRZXTdPvsR8tJJ0d3H2j3E2onuEGsRvH_0nvsRhyf8T47qDoAupuw</recordid><startdate>20160601</startdate><enddate>20160601</enddate><creator>Dunlap, Christopher A</creator><creator>Bowman, Michael J</creator><creator>Schisler, David A</creator><creator>Rooney, Alejandro P</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20160601</creationdate><title>Genome analysis shows Bacillus axarquiensis is not a later heterotypic synonym of Bacillus mojavensis; reclassification of Bacillus malacitensis and Brevibacterium halotolerans as heterotypic synonyms of Bacillus axarquiensis</title><author>Dunlap, Christopher A ; Bowman, Michael J ; Schisler, David A ; Rooney, Alejandro P</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c335t-77dfe337bf6b1ab59c353ffe5648f7599ec64c68503608310158c671ded29c893</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Bacillus - classification</topic><topic>Bacterial Typing Techniques</topic><topic>Base Composition</topic><topic>Brevibacterium - genetics</topic><topic>DNA, Bacterial - genetics</topic><topic>Genome, Bacterial</topic><topic>Nucleic Acid Hybridization</topic><topic>Phylogeny</topic><topic>RNA, Ribosomal, 16S - genetics</topic><topic>Sequence Analysis, DNA</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dunlap, Christopher A</creatorcontrib><creatorcontrib>Bowman, Michael J</creatorcontrib><creatorcontrib>Schisler, David A</creatorcontrib><creatorcontrib>Rooney, Alejandro P</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>International journal of systematic and evolutionary microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dunlap, Christopher A</au><au>Bowman, Michael J</au><au>Schisler, David A</au><au>Rooney, Alejandro P</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genome analysis shows Bacillus axarquiensis is not a later heterotypic synonym of Bacillus mojavensis; reclassification of Bacillus malacitensis and Brevibacterium halotolerans as heterotypic synonyms of Bacillus axarquiensis</atitle><jtitle>International journal of systematic and evolutionary microbiology</jtitle><addtitle>Int J Syst Evol Microbiol</addtitle><date>2016-06-01</date><risdate>2016</risdate><volume>66</volume><issue>6</issue><spage>2438</spage><epage>2443</epage><pages>2438-2443</pages><issn>1466-5026</issn><eissn>1466-5034</eissn><abstract>Bacillus axarquiensis and Bacillus malacitensis were previously reported to be later heterotypic synonyms of Bacillus mojavensis, based primarily on DNA-DNA relatedness values. 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While the pairwise in silico DNA-DNA hybridization values calculated in comparisons of the three conspecific strains with Bacillus mojavensis were all less than 65 %. The combined results of our genotype and phenotype studies showed that Bacillus axarquiensis, Bacillus malacitensis and Brevibacterium halotolerans are conspecific and distinct from Bacillus mojavensis. Because the valid publication of the name Bacillus axarquiensis predates the publication of the name Bacillus malacitensis, we propose that Bacillus malacitensis be reclassified as a synonym of Bacillus axarquiensis. In addition, we propose to reclassify Brevibacterium halotolerans as a synonym of Bacillus axarquiensis. An amended description of Bacillus axarquiensis is provided.</abstract><cop>England</cop><pmid>27030978</pmid><doi>10.1099/ijsem.0.001048</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Bacillus - classification Bacterial Typing Techniques Base Composition Brevibacterium - genetics DNA, Bacterial - genetics Genome, Bacterial Nucleic Acid Hybridization Phylogeny RNA, Ribosomal, 16S - genetics Sequence Analysis, DNA |
| title | Genome analysis shows Bacillus axarquiensis is not a later heterotypic synonym of Bacillus mojavensis; reclassification of Bacillus malacitensis and Brevibacterium halotolerans as heterotypic synonyms of Bacillus axarquiensis |
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