Genotoxicity testing of a fenugreek extract
Fenugreek seeds have been used in traditional medicines as a remedy for diabetes. Rich in protein, fenugreek seeds contain the unique major free amino acid 4-hydroxyisoleucine (4-OH-Ile), which has been characterized as one of the active ingredients for blood glucose control. Current use of fenugree...
Gespeichert in:
| Veröffentlicht in: | Food and chemical toxicology 2004-11, Vol.42 (11), p.1769-1775 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1775 |
|---|---|
| container_issue | 11 |
| container_start_page | 1769 |
| container_title | Food and chemical toxicology |
| container_volume | 42 |
| creator | Flammang, A.M. Cifone, M.A. Erexson, G.L. Stankowski, L.F. |
| description | Fenugreek seeds have been used in traditional medicines as a remedy for diabetes. Rich in protein, fenugreek seeds contain the unique major free amino acid 4-hydroxyisoleucine (4-OH-Ile), which has been characterized as one of the active ingredients for blood glucose control. Current use of fenugreek in foodstuff has been limited to its role as a flavoring agent, and not as an ingredient to help mitigate the blood glucose response for people with diabetes. As part of a safety evaluation of novel ingredients for use in blood glucose control, the potential genotoxicity of a fenugreek seed extract (THL), containing a minimum of 40% 4-OH-ILE, was evaluated using the standard battery of tests (reverse mutation assay; mouse lymphoma forward mutation assay; mouse micronucleus assay) recommended by US Food and Drug Administration (FDA) for food ingredients. THL was determined not to be genotoxic under the conditions of the tested genetic toxicity battery. The negative assay results provide support that addition of THL to foodstuffs formulated for people with diabetes is expected to be safe. A wide safety margin is established, as anticipated doses are small compared to the doses administered in the assays. |
| doi_str_mv | 10.1016/j.fct.2004.07.003 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_18051969</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0278691504002030</els_id><sourcerecordid>18051969</sourcerecordid><originalsourceid>FETCH-LOGICAL-c500t-81b4f50e32f28b1de3212f9f26fd7b32f9acc0a26103443d7768e8b5cb9774c63</originalsourceid><addsrcrecordid>eNp9kMFqGzEQQEVpqN0kH9BLu5f0EnY7I-1KWnoqoXUCgRzSnIVWOzJy7V1XWofk7yNjg285aRBvhsdj7AtChYDyx6rybqo4QF2BqgDEBzZHrUQpRYMf2Ry40qVssZmxzymtAEChkp_YDBvRgFT1nF0vaBin8SW4ML0WE6UpDMti9IUtPA27ZST6V9DLFK2bLtiZt-tEl8f3nD39-f335ra8f1jc3fy6L10DMJUau9o3QIJ7rjvs84Dct55L36su_7bWObBcIoi6Fr1SUpPuGte1StVOinP2_XB3G8f_u6xkNiE5Wq_tQOMuGdTQYCvbDOIBdHFMKZI32xg2Nr4aBLMvZFYmFzL7QgaUyYXyztfj8V23of60cUySgasjYJOzax_t4EI6cXtt0Dxz3w6ct6Oxy5iZp0cOKACym5aQiZ8HgnKs50DRJBdocNSHSFmrH8M7om9-cYr0</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>18051969</pqid></control><display><type>article</type><title>Genotoxicity testing of a fenugreek extract</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals Complete</source><creator>Flammang, A.M. ; Cifone, M.A. ; Erexson, G.L. ; Stankowski, L.F.</creator><creatorcontrib>Flammang, A.M. ; Cifone, M.A. ; Erexson, G.L. ; Stankowski, L.F.</creatorcontrib><description>Fenugreek seeds have been used in traditional medicines as a remedy for diabetes. Rich in protein, fenugreek seeds contain the unique major free amino acid 4-hydroxyisoleucine (4-OH-Ile), which has been characterized as one of the active ingredients for blood glucose control. Current use of fenugreek in foodstuff has been limited to its role as a flavoring agent, and not as an ingredient to help mitigate the blood glucose response for people with diabetes. As part of a safety evaluation of novel ingredients for use in blood glucose control, the potential genotoxicity of a fenugreek seed extract (THL), containing a minimum of 40% 4-OH-ILE, was evaluated using the standard battery of tests (reverse mutation assay; mouse lymphoma forward mutation assay; mouse micronucleus assay) recommended by US Food and Drug Administration (FDA) for food ingredients. THL was determined not to be genotoxic under the conditions of the tested genetic toxicity battery. The negative assay results provide support that addition of THL to foodstuffs formulated for people with diabetes is expected to be safe. A wide safety margin is established, as anticipated doses are small compared to the doses administered in the assays.</description><identifier>ISSN: 0278-6915</identifier><identifier>EISSN: 1873-6351</identifier><identifier>DOI: 10.1016/j.fct.2004.07.003</identifier><identifier>PMID: 15350674</identifier><identifier>CODEN: FCTOD7</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Animals ; Biological and medical sciences ; Blood Glucose - drug effects ; Blood Glucose - metabolism ; cell culture ; cell lines ; chemical composition ; Consumer Product Safety ; Diabetes Mellitus - drug therapy ; Diabetes Mellitus - metabolism ; DNA damage ; dose response ; Dose-Response Relationship, Drug ; Escherichia coli ; Extract ; Fenugreek ; flavorings ; free amino acids ; genotoxicity ; glycemic control ; glycemic effect ; herbs ; Humans ; Hypoglycemic Agents - therapeutic use ; Hypoglycemic Agents - toxicity ; ingredients ; lymphoma ; Male ; Medical sciences ; medicinal plants ; Mice ; Mice, Inbred Strains ; Micronucleus Tests ; Mouse lymphoma forward mutation assay ; Mouse micronucleus assay ; Mutagenicity Tests ; No-Observed-Adverse-Effect Level ; Plant Extracts - toxicity ; Plants, Medicinal ; Reverse mutation assay ; Safety ; safety testing ; Salmonella typhimurium ; seed extracts ; Seeds - toxicity ; toxicity testing ; Toxicology ; traditional medicine ; Trigonella - toxicity ; Trigonella foenum graecum ; United States ; United States Food and Drug Administration ; viability</subject><ispartof>Food and chemical toxicology, 2004-11, Vol.42 (11), p.1769-1775</ispartof><rights>2004 Elsevier Ltd</rights><rights>2004 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c500t-81b4f50e32f28b1de3212f9f26fd7b32f9acc0a26103443d7768e8b5cb9774c63</citedby><cites>FETCH-LOGICAL-c500t-81b4f50e32f28b1de3212f9f26fd7b32f9acc0a26103443d7768e8b5cb9774c63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.fct.2004.07.003$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16103082$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15350674$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Flammang, A.M.</creatorcontrib><creatorcontrib>Cifone, M.A.</creatorcontrib><creatorcontrib>Erexson, G.L.</creatorcontrib><creatorcontrib>Stankowski, L.F.</creatorcontrib><title>Genotoxicity testing of a fenugreek extract</title><title>Food and chemical toxicology</title><addtitle>Food Chem Toxicol</addtitle><description>Fenugreek seeds have been used in traditional medicines as a remedy for diabetes. Rich in protein, fenugreek seeds contain the unique major free amino acid 4-hydroxyisoleucine (4-OH-Ile), which has been characterized as one of the active ingredients for blood glucose control. Current use of fenugreek in foodstuff has been limited to its role as a flavoring agent, and not as an ingredient to help mitigate the blood glucose response for people with diabetes. As part of a safety evaluation of novel ingredients for use in blood glucose control, the potential genotoxicity of a fenugreek seed extract (THL), containing a minimum of 40% 4-OH-ILE, was evaluated using the standard battery of tests (reverse mutation assay; mouse lymphoma forward mutation assay; mouse micronucleus assay) recommended by US Food and Drug Administration (FDA) for food ingredients. THL was determined not to be genotoxic under the conditions of the tested genetic toxicity battery. The negative assay results provide support that addition of THL to foodstuffs formulated for people with diabetes is expected to be safe. A wide safety margin is established, as anticipated doses are small compared to the doses administered in the assays.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Blood Glucose - drug effects</subject><subject>Blood Glucose - metabolism</subject><subject>cell culture</subject><subject>cell lines</subject><subject>chemical composition</subject><subject>Consumer Product Safety</subject><subject>Diabetes Mellitus - drug therapy</subject><subject>Diabetes Mellitus - metabolism</subject><subject>DNA damage</subject><subject>dose response</subject><subject>Dose-Response Relationship, Drug</subject><subject>Escherichia coli</subject><subject>Extract</subject><subject>Fenugreek</subject><subject>flavorings</subject><subject>free amino acids</subject><subject>genotoxicity</subject><subject>glycemic control</subject><subject>glycemic effect</subject><subject>herbs</subject><subject>Humans</subject><subject>Hypoglycemic Agents - therapeutic use</subject><subject>Hypoglycemic Agents - toxicity</subject><subject>ingredients</subject><subject>lymphoma</subject><subject>Male</subject><subject>Medical sciences</subject><subject>medicinal plants</subject><subject>Mice</subject><subject>Mice, Inbred Strains</subject><subject>Micronucleus Tests</subject><subject>Mouse lymphoma forward mutation assay</subject><subject>Mouse micronucleus assay</subject><subject>Mutagenicity Tests</subject><subject>No-Observed-Adverse-Effect Level</subject><subject>Plant Extracts - toxicity</subject><subject>Plants, Medicinal</subject><subject>Reverse mutation assay</subject><subject>Safety</subject><subject>safety testing</subject><subject>Salmonella typhimurium</subject><subject>seed extracts</subject><subject>Seeds - toxicity</subject><subject>toxicity testing</subject><subject>Toxicology</subject><subject>traditional medicine</subject><subject>Trigonella - toxicity</subject><subject>Trigonella foenum graecum</subject><subject>United States</subject><subject>United States Food and Drug Administration</subject><subject>viability</subject><issn>0278-6915</issn><issn>1873-6351</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMFqGzEQQEVpqN0kH9BLu5f0EnY7I-1KWnoqoXUCgRzSnIVWOzJy7V1XWofk7yNjg285aRBvhsdj7AtChYDyx6rybqo4QF2BqgDEBzZHrUQpRYMf2Ry40qVssZmxzymtAEChkp_YDBvRgFT1nF0vaBin8SW4ML0WE6UpDMti9IUtPA27ZST6V9DLFK2bLtiZt-tEl8f3nD39-f335ra8f1jc3fy6L10DMJUau9o3QIJ7rjvs84Dct55L36su_7bWObBcIoi6Fr1SUpPuGte1StVOinP2_XB3G8f_u6xkNiE5Wq_tQOMuGdTQYCvbDOIBdHFMKZI32xg2Nr4aBLMvZFYmFzL7QgaUyYXyztfj8V23of60cUySgasjYJOzax_t4EI6cXtt0Dxz3w6ct6Oxy5iZp0cOKACym5aQiZ8HgnKs50DRJBdocNSHSFmrH8M7om9-cYr0</recordid><startdate>20041101</startdate><enddate>20041101</enddate><creator>Flammang, A.M.</creator><creator>Cifone, M.A.</creator><creator>Erexson, G.L.</creator><creator>Stankowski, L.F.</creator><general>Elsevier Ltd</general><general>Elsevier Science</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T2</scope><scope>7U2</scope><scope>7U7</scope><scope>C1K</scope></search><sort><creationdate>20041101</creationdate><title>Genotoxicity testing of a fenugreek extract</title><author>Flammang, A.M. ; Cifone, M.A. ; Erexson, G.L. ; Stankowski, L.F.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c500t-81b4f50e32f28b1de3212f9f26fd7b32f9acc0a26103443d7768e8b5cb9774c63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Blood Glucose - drug effects</topic><topic>Blood Glucose - metabolism</topic><topic>cell culture</topic><topic>cell lines</topic><topic>chemical composition</topic><topic>Consumer Product Safety</topic><topic>Diabetes Mellitus - drug therapy</topic><topic>Diabetes Mellitus - metabolism</topic><topic>DNA damage</topic><topic>dose response</topic><topic>Dose-Response Relationship, Drug</topic><topic>Escherichia coli</topic><topic>Extract</topic><topic>Fenugreek</topic><topic>flavorings</topic><topic>free amino acids</topic><topic>genotoxicity</topic><topic>glycemic control</topic><topic>glycemic effect</topic><topic>herbs</topic><topic>Humans</topic><topic>Hypoglycemic Agents - therapeutic use</topic><topic>Hypoglycemic Agents - toxicity</topic><topic>ingredients</topic><topic>lymphoma</topic><topic>Male</topic><topic>Medical sciences</topic><topic>medicinal plants</topic><topic>Mice</topic><topic>Mice, Inbred Strains</topic><topic>Micronucleus Tests</topic><topic>Mouse lymphoma forward mutation assay</topic><topic>Mouse micronucleus assay</topic><topic>Mutagenicity Tests</topic><topic>No-Observed-Adverse-Effect Level</topic><topic>Plant Extracts - toxicity</topic><topic>Plants, Medicinal</topic><topic>Reverse mutation assay</topic><topic>Safety</topic><topic>safety testing</topic><topic>Salmonella typhimurium</topic><topic>seed extracts</topic><topic>Seeds - toxicity</topic><topic>toxicity testing</topic><topic>Toxicology</topic><topic>traditional medicine</topic><topic>Trigonella - toxicity</topic><topic>Trigonella foenum graecum</topic><topic>United States</topic><topic>United States Food and Drug Administration</topic><topic>viability</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Flammang, A.M.</creatorcontrib><creatorcontrib>Cifone, M.A.</creatorcontrib><creatorcontrib>Erexson, G.L.</creatorcontrib><creatorcontrib>Stankowski, L.F.</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Health and Safety Science Abstracts (Full archive)</collection><collection>Safety Science and Risk</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Food and chemical toxicology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Flammang, A.M.</au><au>Cifone, M.A.</au><au>Erexson, G.L.</au><au>Stankowski, L.F.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genotoxicity testing of a fenugreek extract</atitle><jtitle>Food and chemical toxicology</jtitle><addtitle>Food Chem Toxicol</addtitle><date>2004-11-01</date><risdate>2004</risdate><volume>42</volume><issue>11</issue><spage>1769</spage><epage>1775</epage><pages>1769-1775</pages><issn>0278-6915</issn><eissn>1873-6351</eissn><coden>FCTOD7</coden><abstract>Fenugreek seeds have been used in traditional medicines as a remedy for diabetes. Rich in protein, fenugreek seeds contain the unique major free amino acid 4-hydroxyisoleucine (4-OH-Ile), which has been characterized as one of the active ingredients for blood glucose control. Current use of fenugreek in foodstuff has been limited to its role as a flavoring agent, and not as an ingredient to help mitigate the blood glucose response for people with diabetes. As part of a safety evaluation of novel ingredients for use in blood glucose control, the potential genotoxicity of a fenugreek seed extract (THL), containing a minimum of 40% 4-OH-ILE, was evaluated using the standard battery of tests (reverse mutation assay; mouse lymphoma forward mutation assay; mouse micronucleus assay) recommended by US Food and Drug Administration (FDA) for food ingredients. THL was determined not to be genotoxic under the conditions of the tested genetic toxicity battery. The negative assay results provide support that addition of THL to foodstuffs formulated for people with diabetes is expected to be safe. A wide safety margin is established, as anticipated doses are small compared to the doses administered in the assays.</abstract><cop>Oxford</cop><cop>New York, NY</cop><pub>Elsevier Ltd</pub><pmid>15350674</pmid><doi>10.1016/j.fct.2004.07.003</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0278-6915 |
| ispartof | Food and chemical toxicology, 2004-11, Vol.42 (11), p.1769-1775 |
| issn | 0278-6915 1873-6351 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_18051969 |
| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Animals Biological and medical sciences Blood Glucose - drug effects Blood Glucose - metabolism cell culture cell lines chemical composition Consumer Product Safety Diabetes Mellitus - drug therapy Diabetes Mellitus - metabolism DNA damage dose response Dose-Response Relationship, Drug Escherichia coli Extract Fenugreek flavorings free amino acids genotoxicity glycemic control glycemic effect herbs Humans Hypoglycemic Agents - therapeutic use Hypoglycemic Agents - toxicity ingredients lymphoma Male Medical sciences medicinal plants Mice Mice, Inbred Strains Micronucleus Tests Mouse lymphoma forward mutation assay Mouse micronucleus assay Mutagenicity Tests No-Observed-Adverse-Effect Level Plant Extracts - toxicity Plants, Medicinal Reverse mutation assay Safety safety testing Salmonella typhimurium seed extracts Seeds - toxicity toxicity testing Toxicology traditional medicine Trigonella - toxicity Trigonella foenum graecum United States United States Food and Drug Administration viability |
| title | Genotoxicity testing of a fenugreek extract |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T13%3A21%3A19IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Genotoxicity%20testing%20of%20a%20fenugreek%20extract&rft.jtitle=Food%20and%20chemical%20toxicology&rft.au=Flammang,%20A.M.&rft.date=2004-11-01&rft.volume=42&rft.issue=11&rft.spage=1769&rft.epage=1775&rft.pages=1769-1775&rft.issn=0278-6915&rft.eissn=1873-6351&rft.coden=FCTOD7&rft_id=info:doi/10.1016/j.fct.2004.07.003&rft_dat=%3Cproquest_cross%3E18051969%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=18051969&rft_id=info:pmid/15350674&rft_els_id=S0278691504002030&rfr_iscdi=true |