Protein-observed (19)F-NMR for fragment screening, affinity quantification and druggability assessment

NMR spectroscopy can be used to quantify the binding affinity between proteins and low-complexity molecules, termed 'fragments'; this versatile screening approach allows researchers to assess the druggability of new protein targets. Protein-observed (19)F-NMR (PrOF NMR) using (19)F-labeled...

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Veröffentlicht in:	Nature protocols 2016-08, Vol.11 (8), p.1414-1427
Hauptverfasser:	Gee, Clifford T, Arntson, Keith E, Urick, Andrew K, Mishra, Neeraj K, Hawk, Laura M L, Wisniewski, Andrea J, Pomerantz, William C K
Format:	Artikel
Sprache:	eng
Schlagworte:	Analysis Halogenation Ligands Magnetic Resonance Spectroscopy - methods Models, Molecular Nuclear magnetic resonance spectroscopy Pharmaceutical Preparations - metabolism Protein binding Protein Conformation Proteins Proteins - chemistry Proteins - metabolism
Online-Zugang:	Volltext
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