The sea anemone Bunodosoma granulifera contains surprisingly efficacious and potent insect-selective toxins
Two sodium channel toxins, BgII and BgIII, isolated from the sea anemone Bunodosoma granulifera, have been subjected to an elaborate electrophysiological and pharmacological comparison between five different cloned sodium channels expressed in Xenopus laevis oocytes in order to determine their effic...
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| Veröffentlicht in: | FEBS letters 2002-12, Vol.532 (1), p.131-134 |
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| creator | Bosmans, Frank Aneiros, Abel Tytgat, Jan |
| description | Two sodium channel toxins,
BgII and
BgIII, isolated from the sea anemone
Bunodosoma granulifera, have been subjected to an elaborate electrophysiological and pharmacological comparison between five different cloned sodium channels expressed in
Xenopus laevis oocytes in order to determine their efficacy, potency and selectivity. Our results reveal large differences in toxin-induced effect between the different sodium channels. These toxins possess the highest efficacy for the insect sodium channel (para). Our data also show that
BgII, generally known as a neurotoxin, is especially potent on the insect sodium channel with an EC
50 value of 5.5±0.5 nM. Therefore, this toxin can be used as a template for further development of new insecticides. Based on our findings, an evolutionary relationship between crustaceans and insects is also discussed. |
| doi_str_mv | 10.1016/S0014-5793(02)03653-0 |
| format | Article |
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BgII and
BgIII, isolated from the sea anemone
Bunodosoma granulifera, have been subjected to an elaborate electrophysiological and pharmacological comparison between five different cloned sodium channels expressed in
Xenopus laevis oocytes in order to determine their efficacy, potency and selectivity. Our results reveal large differences in toxin-induced effect between the different sodium channels. These toxins possess the highest efficacy for the insect sodium channel (para). Our data also show that
BgII, generally known as a neurotoxin, is especially potent on the insect sodium channel with an EC
50 value of 5.5±0.5 nM. Therefore, this toxin can be used as a template for further development of new insecticides. Based on our findings, an evolutionary relationship between crustaceans and insects is also discussed.</description><identifier>ISSN: 0014-5793</identifier><identifier>EISSN: 1873-3468</identifier><identifier>DOI: 10.1016/S0014-5793(02)03653-0</identifier><identifier>PMID: 12459477</identifier><language>eng</language><publisher>England: Elsevier B.V</publisher><subject>Amino Acid Sequence ; Animals ; Bunodosoma granulifera ; Cells, Cultured ; Cnidarian Venoms - genetics ; Cnidarian Venoms - pharmacology ; Dose-Response Relationship, Drug ; Evolution ; Insecta ; Insecticide ; Insecticides - pharmacology ; Marine ; Molecular Sequence Data ; Neurotoxin ; Neurotoxins - genetics ; Neurotoxins - pharmacology ; Oocytes - drug effects ; Patch-Clamp Techniques ; Sea anemone toxin ; Sea Anemones ; Sequence Homology, Amino Acid ; Sodium Channel Blockers - pharmacology ; Voltage-gated sodium channel ; Xenopus laevis</subject><ispartof>FEBS letters, 2002-12, Vol.532 (1), p.131-134</ispartof><rights>2002 Federation of European Biochemical Societies</rights><rights>FEBS Letters 532 (2002) 1873-3468 © 2015 Federation of European Biochemical Societies</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5700-23349bbe2a843f94a66f99daadf1186dcad4c01f25a3863d906676da036e43</citedby><cites>FETCH-LOGICAL-c5700-23349bbe2a843f94a66f99daadf1186dcad4c01f25a3863d906676da036e43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1016%2FS0014-5793%2802%2903653-0$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0014579302036530$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,776,780,1411,1427,3537,27901,27902,45550,45551,46384,46808,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12459477$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bosmans, Frank</creatorcontrib><creatorcontrib>Aneiros, Abel</creatorcontrib><creatorcontrib>Tytgat, Jan</creatorcontrib><title>The sea anemone Bunodosoma granulifera contains surprisingly efficacious and potent insect-selective toxins</title><title>FEBS letters</title><addtitle>FEBS Lett</addtitle><description>Two sodium channel toxins,
BgII and
BgIII, isolated from the sea anemone
Bunodosoma granulifera, have been subjected to an elaborate electrophysiological and pharmacological comparison between five different cloned sodium channels expressed in
Xenopus laevis oocytes in order to determine their efficacy, potency and selectivity. Our results reveal large differences in toxin-induced effect between the different sodium channels. These toxins possess the highest efficacy for the insect sodium channel (para). Our data also show that
BgII, generally known as a neurotoxin, is especially potent on the insect sodium channel with an EC
50 value of 5.5±0.5 nM. Therefore, this toxin can be used as a template for further development of new insecticides. Based on our findings, an evolutionary relationship between crustaceans and insects is also discussed.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Bunodosoma granulifera</subject><subject>Cells, Cultured</subject><subject>Cnidarian Venoms - genetics</subject><subject>Cnidarian Venoms - pharmacology</subject><subject>Dose-Response Relationship, Drug</subject><subject>Evolution</subject><subject>Insecta</subject><subject>Insecticide</subject><subject>Insecticides - pharmacology</subject><subject>Marine</subject><subject>Molecular Sequence Data</subject><subject>Neurotoxin</subject><subject>Neurotoxins - genetics</subject><subject>Neurotoxins - pharmacology</subject><subject>Oocytes - drug effects</subject><subject>Patch-Clamp Techniques</subject><subject>Sea anemone toxin</subject><subject>Sea Anemones</subject><subject>Sequence Homology, Amino Acid</subject><subject>Sodium Channel Blockers - pharmacology</subject><subject>Voltage-gated sodium channel</subject><subject>Xenopus laevis</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkMFu1DAQhi0EokvhEUA-IXpIO44dJzkhWrW0UqVK0LvltcfFkNiLnRT27evsruAIp9HM_PPPzEfIWwanDJg8-wrARNW0Pf8A9Qlw2fAKnpEV61pecSG752T1R3JEXuX8HUresf4lOWK1aHrRtivy4_4b0oya6oBjDEjP5xBtzHHU9CHpMA_eYdLUxDBpHzLNc9okn314GLYUnfNGGx_nXAws3cQJw0SLDs1UZRxK8I9Ip_i71F6TF04PGd8c4jH5cnV5f3Fd3d59vrn4dFuZpgWoas5Fv15jrTvBXS-0lK7vrdbWMdZJa7QVBpirG807yW0PUrbS6sIABT8m7_emmxR_zpgnNfpscBjKg-VMxToQZQCKsNkLTYo5J3Sq_DXqtFUM1IJY7RCrhZ-CWu0Qq2Xu3WHBvB7R_p06MC2C673glx9w-3-u6uryvN51lgbUu_Ky6-PeCguuR49JZeMxGLQ-FbTKRv-Pa58AaoahcQ</recordid><startdate>20021204</startdate><enddate>20021204</enddate><creator>Bosmans, Frank</creator><creator>Aneiros, Abel</creator><creator>Tytgat, Jan</creator><general>Elsevier B.V</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>F1W</scope><scope>FR3</scope><scope>H95</scope><scope>H99</scope><scope>L.F</scope><scope>L.G</scope><scope>P64</scope></search><sort><creationdate>20021204</creationdate><title>The sea anemone Bunodosoma granulifera contains surprisingly efficacious and potent insect-selective toxins</title><author>Bosmans, Frank ; Aneiros, Abel ; Tytgat, Jan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5700-23349bbe2a843f94a66f99daadf1186dcad4c01f25a3863d906676da036e43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Bunodosoma granulifera</topic><topic>Cells, Cultured</topic><topic>Cnidarian Venoms - genetics</topic><topic>Cnidarian Venoms - pharmacology</topic><topic>Dose-Response Relationship, Drug</topic><topic>Evolution</topic><topic>Insecta</topic><topic>Insecticide</topic><topic>Insecticides - pharmacology</topic><topic>Marine</topic><topic>Molecular Sequence Data</topic><topic>Neurotoxin</topic><topic>Neurotoxins - genetics</topic><topic>Neurotoxins - pharmacology</topic><topic>Oocytes - drug effects</topic><topic>Patch-Clamp Techniques</topic><topic>Sea anemone toxin</topic><topic>Sea Anemones</topic><topic>Sequence Homology, Amino Acid</topic><topic>Sodium Channel Blockers - pharmacology</topic><topic>Voltage-gated sodium channel</topic><topic>Xenopus laevis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bosmans, Frank</creatorcontrib><creatorcontrib>Aneiros, Abel</creatorcontrib><creatorcontrib>Tytgat, Jan</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>ASFA: Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bosmans, Frank</au><au>Aneiros, Abel</au><au>Tytgat, Jan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The sea anemone Bunodosoma granulifera contains surprisingly efficacious and potent insect-selective toxins</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>2002-12-04</date><risdate>2002</risdate><volume>532</volume><issue>1</issue><spage>131</spage><epage>134</epage><pages>131-134</pages><issn>0014-5793</issn><eissn>1873-3468</eissn><abstract>Two sodium channel toxins,
BgII and
BgIII, isolated from the sea anemone
Bunodosoma granulifera, have been subjected to an elaborate electrophysiological and pharmacological comparison between five different cloned sodium channels expressed in
Xenopus laevis oocytes in order to determine their efficacy, potency and selectivity. Our results reveal large differences in toxin-induced effect between the different sodium channels. These toxins possess the highest efficacy for the insect sodium channel (para). Our data also show that
BgII, generally known as a neurotoxin, is especially potent on the insect sodium channel with an EC
50 value of 5.5±0.5 nM. Therefore, this toxin can be used as a template for further development of new insecticides. Based on our findings, an evolutionary relationship between crustaceans and insects is also discussed.</abstract><cop>England</cop><pub>Elsevier B.V</pub><pmid>12459477</pmid><doi>10.1016/S0014-5793(02)03653-0</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | FEBS letters, 2002-12, Vol.532 (1), p.131-134 |
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| language | eng |
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| source | Wiley Free Content; MEDLINE; Wiley Online Library Journals Frontfile Complete; Elsevier ScienceDirect Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Amino Acid Sequence Animals Bunodosoma granulifera Cells, Cultured Cnidarian Venoms - genetics Cnidarian Venoms - pharmacology Dose-Response Relationship, Drug Evolution Insecta Insecticide Insecticides - pharmacology Marine Molecular Sequence Data Neurotoxin Neurotoxins - genetics Neurotoxins - pharmacology Oocytes - drug effects Patch-Clamp Techniques Sea anemone toxin Sea Anemones Sequence Homology, Amino Acid Sodium Channel Blockers - pharmacology Voltage-gated sodium channel Xenopus laevis |
| title | The sea anemone Bunodosoma granulifera contains surprisingly efficacious and potent insect-selective toxins |
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