Two methods for full-length RNA sequencing for low quantities of cells and single cells

The ability to determine the gene expression pattern in low quantities of cells or single cells is important for resolving a variety of problems in many biological disciplines. A robust description of the expression signature of a single cell requires determination of the full-length sequence of the...

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Veröffentlicht in:	Proceedings of the National Academy of Sciences - PNAS 2013-01, Vol.110 (2), p.594-599
Hauptverfasser:	Pan, Xinghua, Durrett, Russell E., Zhu, Haiying, Tanaka, Yoshiaki, Li, Yumei, Zi, Xiaoyuan, Marjani, Sadie L., Euskirchen, Ghia, Ma, Chao, LaMotte, Robert H., Park, In-Hyun, Snyder, Michael P., Mason, Christopher E., Weissman, Sherman M.
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological Sciences Cells Complementary DNA DNA DNA polymerase DNA primers DNA Primers - genetics ganglia Gene expression Gene Expression Profiling - methods gene expression regulation Genes high-throughput nucleotide sequencing High-Throughput Nucleotide Sequencing - methods Humans K562 Cells Messenger RNA Neurons Nucleic Acid Amplification Techniques - methods polymerase chain reaction Polymerase Chain Reaction - methods Ribonucleic acid RNA RNA, Messenger - genetics Sequencing Single-Cell Analysis - methods Transcriptomes
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