Stoichiometry of SecYEG in the active translocase of Escherichia coli varies with precursor species
We have established a reconstitution system for the translocon SecYEG in proteoliposomes in which 55% of the accessible translocons are active. This level corresponds to the fraction of translocons that are active in vitro when assessed in their native environment of cytoplasmic membrane vesicles. A...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 2013-07, Vol.110 (29), p.11815-11820 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
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| creator | Mao, Chunfeng Cheadle, Carl E. Hardy, Simon J. S. Lilly, Angela A. Suo, Yuying Gari, Raghavendar Reddy Sanganna King, Gavin M. Randall, Linda L. |
| description | We have established a reconstitution system for the translocon SecYEG in proteoliposomes in which 55% of the accessible translocons are active. This level corresponds to the fraction of translocons that are active in vitro when assessed in their native environment of cytoplasmic membrane vesicles. Assays using these robust reconstituted proteoliposomes and cytoplasmic membrane vesicles have revealed that the number of SecYEG units involved in an active translocase depends on the precursor undergoing transfer. The active translocase for the precursor of periplasmic galactose-binding protein contains twice the number of heterotrimeric units of SecYEG as does that for the precursor of outer membrane protein A. |
| doi_str_mv | 10.1073/pnas.1303289110 |
| format | Article |
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Assays using these robust reconstituted proteoliposomes and cytoplasmic membrane vesicles have revealed that the number of SecYEG units involved in an active translocase depends on the precursor undergoing transfer. 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| source | MEDLINE; Jstor Complete Legacy; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Adenosine triphosphatases Binding sites Biological Sciences Calcium-Binding Proteins - metabolism Carbon Radioisotopes - metabolism Cell membranes Cytoplasm E coli Escherichia coli Escherichia coli - enzymology Escherichia coli Proteins - genetics Escherichia coli Proteins - metabolism Hydrolysis Lipids Liposomes Membrane Proteins - genetics Membrane Proteins - metabolism Membranes Microscopy, Atomic Force Monosaccharide Transport Proteins - metabolism outer membrane proteins Periplasmic Binding Proteins - metabolism Protein precursors Protein subunits Protein transport Proteins Proteolipids - metabolism SEC Translocation Channels stoichiometry Sulfur Radioisotopes - metabolism Transport Vesicles - metabolism |
| title | Stoichiometry of SecYEG in the active translocase of Escherichia coli varies with precursor species |
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