Novel bioassay using Bacillus megaterium to detect tetracycline in milk
Tetracyclines are used for the prevention and control of dairy cattle diseases. Residues of these drugs can be excreted into milk. Thus, the aim of this study was to develop a microbiological method using Bacillus megaterium to detect tetracyclines (chlortetracycline, oxytetracycline and tetracyclin...
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| Veröffentlicht in: | Revista argentina de microbiología 2016-04, Vol.48 (2), p.143-146 |
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| description | Tetracyclines are used for the prevention and control of dairy cattle diseases. Residues of these drugs can be excreted into milk. Thus, the aim of this study was to develop a microbiological method using Bacillus megaterium to detect tetracyclines (chlortetracycline, oxytetracycline and tetracycline) in milk. In order to approximate the limits of detection of the bioassay to the Maximum Residue Limit (100μg/l) for milk tetracycline, different concentrations of chloramphenicol (0, 1000, 1500 and 2000μg/l) were tested. The detection limits calculated were similar to the Maximum Residue Limits when a bioassay using B. megaterium ATCC 9885 spores (2.8×108spores/ml) and chloramphenicol (2000μg/l) was utilized. This bioassay detects 105μg/l of chlortetracycline, 100μg/l of oxytetracycline and 134μg/l of tetracycline in 5h. Therefore, this method is suitable to be incorporated into a microbiological multi-residue system for the identification of tetracyclines in milk.
Las tetraciclinas son utilizadas para la prevención y el control de las enfermedades del ganado lechero; los residuos de estos medicamentos pueden ser excretados en la leche. El objetivo de este estudio fue desarrollar un método microbiológico con esporas de Bacillus megaterium para detectar las tetraciclinas en la leche. Con el propósito de aproximar los límites de detección del bioensayo al límite máximo de residuo permitido para tetraciclinas en leche (100μg/l), se analizaron diferentes concentraciones de cloranfenicol (0, 1.000, 1.500 y 2.000μg/l). Los límites de detección son similares a sus respectivos límites máximos de residuos cuando se utiliza un bioensayo con esporas de Bacillus megaterium ATCC 9885 (2,8 x 108 esporas/ml) y cloranfenicol (2.000μg/l). Este bioensayo detectó 105μg/l de clortetraciclina, 100μg/l de oxitetraciclina y 134μg/l de tetraciclina en 5h. Por lo tanto, este método es adecuado para ser incorporado en un sistema microbiológico multirresiduo para la identificación de tetraciclinas en leche. |
| doi_str_mv | 10.1016/j.ram.2016.02.001 |
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Las tetraciclinas son utilizadas para la prevención y el control de las enfermedades del ganado lechero; los residuos de estos medicamentos pueden ser excretados en la leche. El objetivo de este estudio fue desarrollar un método microbiológico con esporas de Bacillus megaterium para detectar las tetraciclinas en la leche. Con el propósito de aproximar los límites de detección del bioensayo al límite máximo de residuo permitido para tetraciclinas en leche (100μg/l), se analizaron diferentes concentraciones de cloranfenicol (0, 1.000, 1.500 y 2.000μg/l). Los límites de detección son similares a sus respectivos límites máximos de residuos cuando se utiliza un bioensayo con esporas de Bacillus megaterium ATCC 9885 (2,8 x 108 esporas/ml) y cloranfenicol (2.000μg/l). Este bioensayo detectó 105μg/l de clortetraciclina, 100μg/l de oxitetraciclina y 134μg/l de tetraciclina en 5h. Por lo tanto, este método es adecuado para ser incorporado en un sistema microbiológico multirresiduo para la identificación de tetraciclinas en leche.</description><identifier>ISSN: 0325-7541</identifier><identifier>ISSN: 1851-7617</identifier><identifier>DOI: 10.1016/j.ram.2016.02.001</identifier><identifier>PMID: 27131738</identifier><language>eng</language><publisher>Argentina: Elsevier España, S.L.U</publisher><subject>Animals ; Anti-Bacterial Agents - analysis ; Anti-Bacterial Agents - pharmacology ; Antibiotics ; Antibióticos ; Argentina ; Bacillus megaterium ; Bacillus megaterium - drug effects ; Bacillus megaterium - physiology ; Bioassay ; Bioensayo ; Biological Assay - methods ; Chloramphenicol - analysis ; Chloramphenicol - pharmacology ; Detección ; Detection ; Drug Residues - analysis ; Drug Residues - pharmacology ; Food Contamination - analysis ; Food Contamination - legislation & jurisprudence ; Leche ; Maximum Allowable Concentration ; MICROBIOLOGY ; Milk ; Milk - chemistry ; Sensitivity and Specificity ; Spores, Bacterial - drug effects ; Tetraciclinas ; Tetracyclines ; Tetracyclines - analysis ; Tetracyclines - pharmacology</subject><ispartof>Revista argentina de microbiología, 2016-04, Vol.48 (2), p.143-146</ispartof><rights>2016 Asociación Argentina de Microbiología</rights><rights>Copyright © 2016 Asociación Argentina de Microbiología. Publicado por Elsevier España, S.L.U. All rights reserved.</rights><rights>This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c392t-23c07163e46614d5904c5049677d174b3f8b8396afc54c1cde62d6d10fadf9f13</citedby><cites>FETCH-LOGICAL-c392t-23c07163e46614d5904c5049677d174b3f8b8396afc54c1cde62d6d10fadf9f13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,776,780,881,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27131738$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tumini, Melisa</creatorcontrib><creatorcontrib>Nagel, Orlando G.</creatorcontrib><creatorcontrib>Molina, Pilar</creatorcontrib><creatorcontrib>Althaus, Rafael L.</creatorcontrib><title>Novel bioassay using Bacillus megaterium to detect tetracycline in milk</title><title>Revista argentina de microbiología</title><addtitle>Rev Argent Microbiol</addtitle><description>Tetracyclines are used for the prevention and control of dairy cattle diseases. Residues of these drugs can be excreted into milk. Thus, the aim of this study was to develop a microbiological method using Bacillus megaterium to detect tetracyclines (chlortetracycline, oxytetracycline and tetracycline) in milk. In order to approximate the limits of detection of the bioassay to the Maximum Residue Limit (100μg/l) for milk tetracycline, different concentrations of chloramphenicol (0, 1000, 1500 and 2000μg/l) were tested. The detection limits calculated were similar to the Maximum Residue Limits when a bioassay using B. megaterium ATCC 9885 spores (2.8×108spores/ml) and chloramphenicol (2000μg/l) was utilized. This bioassay detects 105μg/l of chlortetracycline, 100μg/l of oxytetracycline and 134μg/l of tetracycline in 5h. Therefore, this method is suitable to be incorporated into a microbiological multi-residue system for the identification of tetracyclines in milk.
Las tetraciclinas son utilizadas para la prevención y el control de las enfermedades del ganado lechero; los residuos de estos medicamentos pueden ser excretados en la leche. El objetivo de este estudio fue desarrollar un método microbiológico con esporas de Bacillus megaterium para detectar las tetraciclinas en la leche. Con el propósito de aproximar los límites de detección del bioensayo al límite máximo de residuo permitido para tetraciclinas en leche (100μg/l), se analizaron diferentes concentraciones de cloranfenicol (0, 1.000, 1.500 y 2.000μg/l). Los límites de detección son similares a sus respectivos límites máximos de residuos cuando se utiliza un bioensayo con esporas de Bacillus megaterium ATCC 9885 (2,8 x 108 esporas/ml) y cloranfenicol (2.000μg/l). Este bioensayo detectó 105μg/l de clortetraciclina, 100μg/l de oxitetraciclina y 134μg/l de tetraciclina en 5h. Por lo tanto, este método es adecuado para ser incorporado en un sistema microbiológico multirresiduo para la identificación de tetraciclinas en leche.</description><subject>Animals</subject><subject>Anti-Bacterial Agents - analysis</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Antibiotics</subject><subject>Antibióticos</subject><subject>Argentina</subject><subject>Bacillus megaterium</subject><subject>Bacillus megaterium - drug effects</subject><subject>Bacillus megaterium - physiology</subject><subject>Bioassay</subject><subject>Bioensayo</subject><subject>Biological Assay - methods</subject><subject>Chloramphenicol - analysis</subject><subject>Chloramphenicol - pharmacology</subject><subject>Detección</subject><subject>Detection</subject><subject>Drug Residues - analysis</subject><subject>Drug Residues - pharmacology</subject><subject>Food Contamination - analysis</subject><subject>Food Contamination - legislation & jurisprudence</subject><subject>Leche</subject><subject>Maximum Allowable Concentration</subject><subject>MICROBIOLOGY</subject><subject>Milk</subject><subject>Milk - chemistry</subject><subject>Sensitivity and Specificity</subject><subject>Spores, Bacterial - drug effects</subject><subject>Tetraciclinas</subject><subject>Tetracyclines</subject><subject>Tetracyclines - analysis</subject><subject>Tetracyclines - pharmacology</subject><issn>0325-7541</issn><issn>1851-7617</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kEFP3DAQhX2g6lLaH8AF-chl0xnbSTbqiSKglVB7gJ4trz1BXpyY2slK--9xtNBjD6OZw3tvZj7GzhEqBGy-7qpkhkqUsQJRAeAJOwUp6nVbK1yxTznvABQoKT-ylWhRYis3p-zuV9xT4FsfTc7mwOfsxyf-3Vgfwpz5QE9mouTngU-RO5rITnyiKRl7sMGPxP3IBx-eP7MPvQmZvrz1M_bn9ubx-sf6_vfdz-ur-7WVnZjWQlposZGkmgaVqztQtgbVNW3rsFVb2W-2G9k1pre1smgdNcI1DqE3ru96lGesOuZm6ylEvYtzGstC_bA8q5dnFwYAIEohFMPl0fCS4t-Z8qQHny2FYEaKc9a4AdEq2dVdkeJRalPMOVGvX5IfTDpoBL1A1jtdIOtlgQahS37xXLzFz9uB3D_HO-Ei-HYUUKGy95T0cvloyflUYGoX_X_iXwEUUYt_</recordid><startdate>20160401</startdate><enddate>20160401</enddate><creator>Tumini, Melisa</creator><creator>Nagel, Orlando G.</creator><creator>Molina, Pilar</creator><creator>Althaus, Rafael L.</creator><general>Elsevier España, S.L.U</general><general>Asociación Argentina de Microbiología</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>GPN</scope></search><sort><creationdate>20160401</creationdate><title>Novel bioassay using Bacillus megaterium to detect tetracycline in milk</title><author>Tumini, Melisa ; Nagel, Orlando G. ; Molina, Pilar ; Althaus, Rafael L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c392t-23c07163e46614d5904c5049677d174b3f8b8396afc54c1cde62d6d10fadf9f13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Animals</topic><topic>Anti-Bacterial Agents - analysis</topic><topic>Anti-Bacterial Agents - pharmacology</topic><topic>Antibiotics</topic><topic>Antibióticos</topic><topic>Argentina</topic><topic>Bacillus megaterium</topic><topic>Bacillus megaterium - drug effects</topic><topic>Bacillus megaterium - physiology</topic><topic>Bioassay</topic><topic>Bioensayo</topic><topic>Biological Assay - methods</topic><topic>Chloramphenicol - analysis</topic><topic>Chloramphenicol - pharmacology</topic><topic>Detección</topic><topic>Detection</topic><topic>Drug Residues - analysis</topic><topic>Drug Residues - pharmacology</topic><topic>Food Contamination - analysis</topic><topic>Food Contamination - legislation & jurisprudence</topic><topic>Leche</topic><topic>Maximum Allowable Concentration</topic><topic>MICROBIOLOGY</topic><topic>Milk</topic><topic>Milk - chemistry</topic><topic>Sensitivity and Specificity</topic><topic>Spores, Bacterial - drug effects</topic><topic>Tetraciclinas</topic><topic>Tetracyclines</topic><topic>Tetracyclines - analysis</topic><topic>Tetracyclines - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tumini, Melisa</creatorcontrib><creatorcontrib>Nagel, Orlando G.</creatorcontrib><creatorcontrib>Molina, Pilar</creatorcontrib><creatorcontrib>Althaus, Rafael L.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>SciELO</collection><jtitle>Revista argentina de microbiología</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tumini, Melisa</au><au>Nagel, Orlando G.</au><au>Molina, Pilar</au><au>Althaus, Rafael L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Novel bioassay using Bacillus megaterium to detect tetracycline in milk</atitle><jtitle>Revista argentina de microbiología</jtitle><addtitle>Rev Argent Microbiol</addtitle><date>2016-04-01</date><risdate>2016</risdate><volume>48</volume><issue>2</issue><spage>143</spage><epage>146</epage><pages>143-146</pages><issn>0325-7541</issn><issn>1851-7617</issn><abstract>Tetracyclines are used for the prevention and control of dairy cattle diseases. Residues of these drugs can be excreted into milk. Thus, the aim of this study was to develop a microbiological method using Bacillus megaterium to detect tetracyclines (chlortetracycline, oxytetracycline and tetracycline) in milk. In order to approximate the limits of detection of the bioassay to the Maximum Residue Limit (100μg/l) for milk tetracycline, different concentrations of chloramphenicol (0, 1000, 1500 and 2000μg/l) were tested. The detection limits calculated were similar to the Maximum Residue Limits when a bioassay using B. megaterium ATCC 9885 spores (2.8×108spores/ml) and chloramphenicol (2000μg/l) was utilized. This bioassay detects 105μg/l of chlortetracycline, 100μg/l of oxytetracycline and 134μg/l of tetracycline in 5h. Therefore, this method is suitable to be incorporated into a microbiological multi-residue system for the identification of tetracyclines in milk.
Las tetraciclinas son utilizadas para la prevención y el control de las enfermedades del ganado lechero; los residuos de estos medicamentos pueden ser excretados en la leche. El objetivo de este estudio fue desarrollar un método microbiológico con esporas de Bacillus megaterium para detectar las tetraciclinas en la leche. Con el propósito de aproximar los límites de detección del bioensayo al límite máximo de residuo permitido para tetraciclinas en leche (100μg/l), se analizaron diferentes concentraciones de cloranfenicol (0, 1.000, 1.500 y 2.000μg/l). Los límites de detección son similares a sus respectivos límites máximos de residuos cuando se utiliza un bioensayo con esporas de Bacillus megaterium ATCC 9885 (2,8 x 108 esporas/ml) y cloranfenicol (2.000μg/l). Este bioensayo detectó 105μg/l de clortetraciclina, 100μg/l de oxitetraciclina y 134μg/l de tetraciclina en 5h. Por lo tanto, este método es adecuado para ser incorporado en un sistema microbiológico multirresiduo para la identificación de tetraciclinas en leche.</abstract><cop>Argentina</cop><pub>Elsevier España, S.L.U</pub><pmid>27131738</pmid><doi>10.1016/j.ram.2016.02.001</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Anti-Bacterial Agents - analysis Anti-Bacterial Agents - pharmacology Antibiotics Antibióticos Argentina Bacillus megaterium Bacillus megaterium - drug effects Bacillus megaterium - physiology Bioassay Bioensayo Biological Assay - methods Chloramphenicol - analysis Chloramphenicol - pharmacology Detección Detection Drug Residues - analysis Drug Residues - pharmacology Food Contamination - analysis Food Contamination - legislation & jurisprudence Leche Maximum Allowable Concentration MICROBIOLOGY Milk Milk - chemistry Sensitivity and Specificity Spores, Bacterial - drug effects Tetraciclinas Tetracyclines Tetracyclines - analysis Tetracyclines - pharmacology |
| title | Novel bioassay using Bacillus megaterium to detect tetracycline in milk |
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