Characterization of Fatty Acid Binding Protein 7 (FABP7) in the Murine Retina
To characterize the mouse retina lacking fatty acid binding protein (FABP7-/-). Immunohistochemistry (IHC) was performed in 8-week-old mice to localize FABP7 in the retina. Retinal thickness was measured using image-guided spectral-domain optical coherence topography images. Electroretinography was...
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| Veröffentlicht in: | Investigative ophthalmology & visual science 2016-06, Vol.57 (7), p.3397-3408 |
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| creator | Su, Xinyi Tan, Queenie S W Parikh, Bhav H Tan, Alison Mehta, Milan N Sia Wey, Yeo Tun, Sai Bo Bo Li, Ling-Jun Han, Xiao-Yan Wong, Tien Y Hunziker, Walter Luu, Chi D Owada, Yuji Barathi, Veluchamy A Zhang, Samuel S Chaurasia, Shyam S |
| description | To characterize the mouse retina lacking fatty acid binding protein (FABP7-/-).
Immunohistochemistry (IHC) was performed in 8-week-old mice to localize FABP7 in the retina. Retinal thickness was measured using image-guided spectral-domain optical coherence topography images. Electroretinography was carried out to assess retinal function. Fundus photography and fundus fluorescein angiography were performed on FABP7-/- and littermate wild-type (WT) mice, and retinal vascular changes were calculated using Singapore I Vessel Assessment (SIVA) analysis. Blood glucose levels were measured in the 8-week-old WT and FABP7-/- mice. In addition, retina was processed for trypsin digestion and retinal flat mounts for isolectin staining. Transcript levels of FABP7, VEGF, GFAP, and Na+K+ATPase were quantified using real-time PCR, and protein expression was analyzed by IHC and Western blot.
Fatty acid binding protein 7 is expressed in the inner nuclear layer, outer plexiform layer, and photoreceptor inner segments. No significant difference in retinal thickness and ERG responses was observed between FABP7-deficient and WT retinas. FABP7-/- mice have significantly decreased retinal venular caliber retinal arteriolar fractal dimension compared with WT littermates. FABP7-/- mice showed significant increased areas of fluorescein leakage in the retina. FABP7-/- mice exhibited elevated high blood glucose levels compared with WT mice. Trypsin digested FABP7-/- mice retina showed increased acellular strands and endothelial cell drop outs, and reduced microvasculature branching compared with WT retina. FABP7-/- mice retina also have increased GFAP and VEGF expression.
Fatty acid binding protein 7 is expressed in the retina and might play an important role in maintaining retinal vasculature. |
| doi_str_mv | 10.1167/iovs.15-18542 |
| format | Article |
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Immunohistochemistry (IHC) was performed in 8-week-old mice to localize FABP7 in the retina. Retinal thickness was measured using image-guided spectral-domain optical coherence topography images. Electroretinography was carried out to assess retinal function. Fundus photography and fundus fluorescein angiography were performed on FABP7-/- and littermate wild-type (WT) mice, and retinal vascular changes were calculated using Singapore I Vessel Assessment (SIVA) analysis. Blood glucose levels were measured in the 8-week-old WT and FABP7-/- mice. In addition, retina was processed for trypsin digestion and retinal flat mounts for isolectin staining. Transcript levels of FABP7, VEGF, GFAP, and Na+K+ATPase were quantified using real-time PCR, and protein expression was analyzed by IHC and Western blot.
Fatty acid binding protein 7 is expressed in the inner nuclear layer, outer plexiform layer, and photoreceptor inner segments. No significant difference in retinal thickness and ERG responses was observed between FABP7-deficient and WT retinas. FABP7-/- mice have significantly decreased retinal venular caliber retinal arteriolar fractal dimension compared with WT littermates. FABP7-/- mice showed significant increased areas of fluorescein leakage in the retina. FABP7-/- mice exhibited elevated high blood glucose levels compared with WT mice. Trypsin digested FABP7-/- mice retina showed increased acellular strands and endothelial cell drop outs, and reduced microvasculature branching compared with WT retina. FABP7-/- mice retina also have increased GFAP and VEGF expression.
Fatty acid binding protein 7 is expressed in the retina and might play an important role in maintaining retinal vasculature.</description><identifier>ISSN: 1552-5783</identifier><identifier>EISSN: 1552-5783</identifier><identifier>DOI: 10.1167/iovs.15-18542</identifier><identifier>PMID: 27367508</identifier><language>eng</language><publisher>United States</publisher><subject>Animals ; Blood Glucose - analysis ; Blotting, Western ; Disease Models, Animal ; Electroretinography ; Fatty Acid-Binding Protein 7 - deficiency ; Fatty Acid-Binding Protein 7 - metabolism ; Fluorescein Angiography ; Immunohistochemistry ; Mice ; Mice, Inbred C57BL ; Mice, Knockout ; Real-Time Polymerase Chain Reaction ; Retina - metabolism ; Retina - physiopathology ; Retinal Degeneration - metabolism ; Retinal Degeneration - physiopathology ; Retinal Vessels - physiopathology ; Tomography, Optical Coherence</subject><ispartof>Investigative ophthalmology & visual science, 2016-06, Vol.57 (7), p.3397-3408</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c398t-9626dca19a30a4df19bacdb773e7850a2c1d83ca6fa43af4f71bf684d21620793</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,860,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27367508$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Su, Xinyi</creatorcontrib><creatorcontrib>Tan, Queenie S W</creatorcontrib><creatorcontrib>Parikh, Bhav H</creatorcontrib><creatorcontrib>Tan, Alison</creatorcontrib><creatorcontrib>Mehta, Milan N</creatorcontrib><creatorcontrib>Sia Wey, Yeo</creatorcontrib><creatorcontrib>Tun, Sai Bo Bo</creatorcontrib><creatorcontrib>Li, Ling-Jun</creatorcontrib><creatorcontrib>Han, Xiao-Yan</creatorcontrib><creatorcontrib>Wong, Tien Y</creatorcontrib><creatorcontrib>Hunziker, Walter</creatorcontrib><creatorcontrib>Luu, Chi D</creatorcontrib><creatorcontrib>Owada, Yuji</creatorcontrib><creatorcontrib>Barathi, Veluchamy A</creatorcontrib><creatorcontrib>Zhang, Samuel S</creatorcontrib><creatorcontrib>Chaurasia, Shyam S</creatorcontrib><title>Characterization of Fatty Acid Binding Protein 7 (FABP7) in the Murine Retina</title><title>Investigative ophthalmology & visual science</title><addtitle>Invest Ophthalmol Vis Sci</addtitle><description>To characterize the mouse retina lacking fatty acid binding protein (FABP7-/-).
Immunohistochemistry (IHC) was performed in 8-week-old mice to localize FABP7 in the retina. Retinal thickness was measured using image-guided spectral-domain optical coherence topography images. Electroretinography was carried out to assess retinal function. Fundus photography and fundus fluorescein angiography were performed on FABP7-/- and littermate wild-type (WT) mice, and retinal vascular changes were calculated using Singapore I Vessel Assessment (SIVA) analysis. Blood glucose levels were measured in the 8-week-old WT and FABP7-/- mice. In addition, retina was processed for trypsin digestion and retinal flat mounts for isolectin staining. Transcript levels of FABP7, VEGF, GFAP, and Na+K+ATPase were quantified using real-time PCR, and protein expression was analyzed by IHC and Western blot.
Fatty acid binding protein 7 is expressed in the inner nuclear layer, outer plexiform layer, and photoreceptor inner segments. No significant difference in retinal thickness and ERG responses was observed between FABP7-deficient and WT retinas. FABP7-/- mice have significantly decreased retinal venular caliber retinal arteriolar fractal dimension compared with WT littermates. FABP7-/- mice showed significant increased areas of fluorescein leakage in the retina. FABP7-/- mice exhibited elevated high blood glucose levels compared with WT mice. Trypsin digested FABP7-/- mice retina showed increased acellular strands and endothelial cell drop outs, and reduced microvasculature branching compared with WT retina. FABP7-/- mice retina also have increased GFAP and VEGF expression.
Fatty acid binding protein 7 is expressed in the retina and might play an important role in maintaining retinal vasculature.</description><subject>Animals</subject><subject>Blood Glucose - analysis</subject><subject>Blotting, Western</subject><subject>Disease Models, Animal</subject><subject>Electroretinography</subject><subject>Fatty Acid-Binding Protein 7 - deficiency</subject><subject>Fatty Acid-Binding Protein 7 - metabolism</subject><subject>Fluorescein Angiography</subject><subject>Immunohistochemistry</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Mice, Knockout</subject><subject>Real-Time Polymerase Chain Reaction</subject><subject>Retina - metabolism</subject><subject>Retina - physiopathology</subject><subject>Retinal Degeneration - metabolism</subject><subject>Retinal Degeneration - physiopathology</subject><subject>Retinal Vessels - physiopathology</subject><subject>Tomography, Optical Coherence</subject><issn>1552-5783</issn><issn>1552-5783</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpNkEtPAjEYRRujEUSXbk2XuBjtY2baWQIRNYFIjK6bjz6kBmaw7ZjgrxcEjat7b3JyFwehS0puKC3FrW8-4w0tMiqLnB2hLi0KlhVC8uN_vYPOYnwnhFHKyCnqMMFLURDZRdPRAgLoZIP_guSbGjcOjyGlDR5ob_DQ18bXb3gWmmR9jQXujwfDmbjG25EWFk_b4GuLn23yNZyjEwfLaC8O2UOv47uX0UM2ebp_HA0mmeaVTFlVstJooBVwArlxtJqDNnMhuBWyIMA0NZJrKB3kHFzuBJ27UuaG0ZIRUfEe6u9_16H5aG1MauWjtssl1LZpo6KSsFwQUrEtmu1RHZoYg3VqHfwKwkZRonYG1c6gooX6Mbjlrw7X7XxlzR_9q4x_AwjMato</recordid><startdate>20160601</startdate><enddate>20160601</enddate><creator>Su, Xinyi</creator><creator>Tan, Queenie S W</creator><creator>Parikh, Bhav H</creator><creator>Tan, Alison</creator><creator>Mehta, Milan N</creator><creator>Sia Wey, Yeo</creator><creator>Tun, Sai Bo Bo</creator><creator>Li, Ling-Jun</creator><creator>Han, Xiao-Yan</creator><creator>Wong, Tien Y</creator><creator>Hunziker, Walter</creator><creator>Luu, Chi D</creator><creator>Owada, Yuji</creator><creator>Barathi, Veluchamy A</creator><creator>Zhang, Samuel S</creator><creator>Chaurasia, Shyam S</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20160601</creationdate><title>Characterization of Fatty Acid Binding Protein 7 (FABP7) in the Murine Retina</title><author>Su, Xinyi ; Tan, Queenie S W ; Parikh, Bhav H ; Tan, Alison ; Mehta, Milan N ; Sia Wey, Yeo ; Tun, Sai Bo Bo ; Li, Ling-Jun ; Han, Xiao-Yan ; Wong, Tien Y ; Hunziker, Walter ; Luu, Chi D ; Owada, Yuji ; Barathi, Veluchamy A ; Zhang, Samuel S ; Chaurasia, Shyam S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c398t-9626dca19a30a4df19bacdb773e7850a2c1d83ca6fa43af4f71bf684d21620793</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Animals</topic><topic>Blood Glucose - analysis</topic><topic>Blotting, Western</topic><topic>Disease Models, Animal</topic><topic>Electroretinography</topic><topic>Fatty Acid-Binding Protein 7 - deficiency</topic><topic>Fatty Acid-Binding Protein 7 - metabolism</topic><topic>Fluorescein Angiography</topic><topic>Immunohistochemistry</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Mice, Knockout</topic><topic>Real-Time Polymerase Chain Reaction</topic><topic>Retina - metabolism</topic><topic>Retina - physiopathology</topic><topic>Retinal Degeneration - metabolism</topic><topic>Retinal Degeneration - physiopathology</topic><topic>Retinal Vessels - physiopathology</topic><topic>Tomography, Optical Coherence</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Su, Xinyi</creatorcontrib><creatorcontrib>Tan, Queenie S W</creatorcontrib><creatorcontrib>Parikh, Bhav H</creatorcontrib><creatorcontrib>Tan, Alison</creatorcontrib><creatorcontrib>Mehta, Milan N</creatorcontrib><creatorcontrib>Sia Wey, Yeo</creatorcontrib><creatorcontrib>Tun, Sai Bo Bo</creatorcontrib><creatorcontrib>Li, Ling-Jun</creatorcontrib><creatorcontrib>Han, Xiao-Yan</creatorcontrib><creatorcontrib>Wong, Tien Y</creatorcontrib><creatorcontrib>Hunziker, Walter</creatorcontrib><creatorcontrib>Luu, Chi D</creatorcontrib><creatorcontrib>Owada, Yuji</creatorcontrib><creatorcontrib>Barathi, Veluchamy A</creatorcontrib><creatorcontrib>Zhang, Samuel S</creatorcontrib><creatorcontrib>Chaurasia, Shyam S</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Investigative ophthalmology & visual science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Su, Xinyi</au><au>Tan, Queenie S W</au><au>Parikh, Bhav H</au><au>Tan, Alison</au><au>Mehta, Milan N</au><au>Sia Wey, Yeo</au><au>Tun, Sai Bo Bo</au><au>Li, Ling-Jun</au><au>Han, Xiao-Yan</au><au>Wong, Tien Y</au><au>Hunziker, Walter</au><au>Luu, Chi D</au><au>Owada, Yuji</au><au>Barathi, Veluchamy A</au><au>Zhang, Samuel S</au><au>Chaurasia, Shyam S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization of Fatty Acid Binding Protein 7 (FABP7) in the Murine Retina</atitle><jtitle>Investigative ophthalmology & visual science</jtitle><addtitle>Invest Ophthalmol Vis Sci</addtitle><date>2016-06-01</date><risdate>2016</risdate><volume>57</volume><issue>7</issue><spage>3397</spage><epage>3408</epage><pages>3397-3408</pages><issn>1552-5783</issn><eissn>1552-5783</eissn><abstract>To characterize the mouse retina lacking fatty acid binding protein (FABP7-/-).
Immunohistochemistry (IHC) was performed in 8-week-old mice to localize FABP7 in the retina. Retinal thickness was measured using image-guided spectral-domain optical coherence topography images. Electroretinography was carried out to assess retinal function. Fundus photography and fundus fluorescein angiography were performed on FABP7-/- and littermate wild-type (WT) mice, and retinal vascular changes were calculated using Singapore I Vessel Assessment (SIVA) analysis. Blood glucose levels were measured in the 8-week-old WT and FABP7-/- mice. In addition, retina was processed for trypsin digestion and retinal flat mounts for isolectin staining. Transcript levels of FABP7, VEGF, GFAP, and Na+K+ATPase were quantified using real-time PCR, and protein expression was analyzed by IHC and Western blot.
Fatty acid binding protein 7 is expressed in the inner nuclear layer, outer plexiform layer, and photoreceptor inner segments. No significant difference in retinal thickness and ERG responses was observed between FABP7-deficient and WT retinas. FABP7-/- mice have significantly decreased retinal venular caliber retinal arteriolar fractal dimension compared with WT littermates. FABP7-/- mice showed significant increased areas of fluorescein leakage in the retina. FABP7-/- mice exhibited elevated high blood glucose levels compared with WT mice. Trypsin digested FABP7-/- mice retina showed increased acellular strands and endothelial cell drop outs, and reduced microvasculature branching compared with WT retina. FABP7-/- mice retina also have increased GFAP and VEGF expression.
Fatty acid binding protein 7 is expressed in the retina and might play an important role in maintaining retinal vasculature.</abstract><cop>United States</cop><pmid>27367508</pmid><doi>10.1167/iovs.15-18542</doi><tpages>12</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Animals Blood Glucose - analysis Blotting, Western Disease Models, Animal Electroretinography Fatty Acid-Binding Protein 7 - deficiency Fatty Acid-Binding Protein 7 - metabolism Fluorescein Angiography Immunohistochemistry Mice Mice, Inbred C57BL Mice, Knockout Real-Time Polymerase Chain Reaction Retina - metabolism Retina - physiopathology Retinal Degeneration - metabolism Retinal Degeneration - physiopathology Retinal Vessels - physiopathology Tomography, Optical Coherence |
| title | Characterization of Fatty Acid Binding Protein 7 (FABP7) in the Murine Retina |
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