Lettuce irrigated with contaminated water: Photosynthetic effects, antioxidative response and bioaccumulation of microcystin congeners
The use of microcystins (MCs) contaminated water to irrigate crop plants represents a human health risk due to their bioaccumulation potential. In addition, MCs cause oxidative stress and negatively influence photosynthetic activities in plants. The present study was aimed at investigating the effec...
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| Veröffentlicht in: | Ecotoxicology and environmental safety 2016-06, Vol.128, p.83-90 |
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| creator | Bittencourt-Oliveira, Maria do Carmo Cordeiro-Araújo, Micheline Kézia Chia, Mathias Ahii Arruda-Neto, João Dias de Toledo Oliveira, Ênio Tiago de Santos, Flávio dos |
| description | The use of microcystins (MCs) contaminated water to irrigate crop plants represents a human health risk due to their bioaccumulation potential. In addition, MCs cause oxidative stress and negatively influence photosynthetic activities in plants. The present study was aimed at investigating the effect of MCs on photosynthetic parameters and antioxidative response of lettuce. Furthermore, the bioaccumulation factor (BAF) of total MCs, MC-LR and MC-RR in the vegetable after irrigation with contaminated water was determined. Lettuce crops were irrigated for 15 days with water containing cyanobacterial crude extracts (Microcystis aeruginosa) with MC-LR (0.0, 0.5, 2.0, 5.0 and 10.0µgL−1), MC-RR (0.0, 0.15, 0.5, 1.5 and 3.0µgL−1) and total MCs (0.0, 0.65, 2.5, 6.5 and 13.0µgL−1). Increased net photosynthetic rate, stomatal conductance, leaf tissue transpiration and intercellular CO2 concentration were recorded in lettuce exposed to different MCs concentrations. Antioxidant response showed that glutathione S-transferase activity was down-regulated in the presence of MCs. On the other hand, superoxide dismutase, catalase and peroxidase activities were upregulated with increasing MCs concentrations. The bioaccumulation factor (BAF) of total MCs and MC-LR was highest at 6.50 and 5.00µgL−1, respectively, while for MC-RR, the highest BAF was recorded at 1.50µgL−1 concentration. The amount of total MCs, MC-LR and MC-RR bioacumulated in lettuce was highest at the highest exposure concentrations. However, at the lowest exposure concentration, there were no detectable levels of MC-LR, MC-RR and total MCs in lettuce. Thus, the bioaccumulation of MCs in lettuce varies according to the exposure concentration. In addition, the extent of physiological response of lettuce to the toxins relies on exposure concentrations.
[Display omitted]
•Lettuce was irrigated with microcystins (MCs) contaminated water.•Photosynthetic rates and other gas exchange parameters were significantly altered.•Protein content and antioxidant enzyme activities were significantly affected by MCs.•Variation of MCs bioaccumulation factor depended on exposure level and congener type.•Due to accumulated levels, irrigation with MCs contaminated water poses a health risk. |
| doi_str_mv | 10.1016/j.ecoenv.2016.02.014 |
| format | Article |
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[Display omitted]
•Lettuce was irrigated with microcystins (MCs) contaminated water.•Photosynthetic rates and other gas exchange parameters were significantly altered.•Protein content and antioxidant enzyme activities were significantly affected by MCs.•Variation of MCs bioaccumulation factor depended on exposure level and congener type.•Due to accumulated levels, irrigation with MCs contaminated water poses a health risk.</description><identifier>ISSN: 0147-6513</identifier><identifier>EISSN: 1090-2414</identifier><identifier>DOI: 10.1016/j.ecoenv.2016.02.014</identifier><identifier>PMID: 26896895</identifier><language>eng</language><publisher>Netherlands: Elsevier Inc</publisher><subject>Agricultural Irrigation ; Antioxidants - metabolism ; Catalase - metabolism ; Food contamination ; Glutathione Transferase - metabolism ; Human health ; Humans ; Lactuca - drug effects ; Lactuca - growth & development ; Lactuca - metabolism ; Microcystins ; Microcystins - metabolism ; Microcystins - toxicity ; Microcystis - metabolism ; Microcystis aeruginosa ; Oxidative stress ; Oxidative Stress - drug effects ; Photosynthesis ; Photosynthesis - drug effects ; Plant Leaves - drug effects ; Plant Leaves - growth & development ; Plant Leaves - metabolism ; Water Pollutants, Chemical - metabolism ; Water Pollutants, Chemical - toxicity</subject><ispartof>Ecotoxicology and environmental safety, 2016-06, Vol.128, p.83-90</ispartof><rights>2016 Elsevier Inc.</rights><rights>Copyright © 2016 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c395t-5c50da362f12f4baa72ddec1f64db65492232de8625003d7104a69a64fc56c7f3</citedby><cites>FETCH-LOGICAL-c395t-5c50da362f12f4baa72ddec1f64db65492232de8625003d7104a69a64fc56c7f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.ecoenv.2016.02.014$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26896895$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bittencourt-Oliveira, Maria do Carmo</creatorcontrib><creatorcontrib>Cordeiro-Araújo, Micheline Kézia</creatorcontrib><creatorcontrib>Chia, Mathias Ahii</creatorcontrib><creatorcontrib>Arruda-Neto, João Dias de Toledo</creatorcontrib><creatorcontrib>Oliveira, Ênio Tiago de</creatorcontrib><creatorcontrib>Santos, Flávio dos</creatorcontrib><title>Lettuce irrigated with contaminated water: Photosynthetic effects, antioxidative response and bioaccumulation of microcystin congeners</title><title>Ecotoxicology and environmental safety</title><addtitle>Ecotoxicol Environ Saf</addtitle><description>The use of microcystins (MCs) contaminated water to irrigate crop plants represents a human health risk due to their bioaccumulation potential. In addition, MCs cause oxidative stress and negatively influence photosynthetic activities in plants. The present study was aimed at investigating the effect of MCs on photosynthetic parameters and antioxidative response of lettuce. Furthermore, the bioaccumulation factor (BAF) of total MCs, MC-LR and MC-RR in the vegetable after irrigation with contaminated water was determined. Lettuce crops were irrigated for 15 days with water containing cyanobacterial crude extracts (Microcystis aeruginosa) with MC-LR (0.0, 0.5, 2.0, 5.0 and 10.0µgL−1), MC-RR (0.0, 0.15, 0.5, 1.5 and 3.0µgL−1) and total MCs (0.0, 0.65, 2.5, 6.5 and 13.0µgL−1). Increased net photosynthetic rate, stomatal conductance, leaf tissue transpiration and intercellular CO2 concentration were recorded in lettuce exposed to different MCs concentrations. Antioxidant response showed that glutathione S-transferase activity was down-regulated in the presence of MCs. On the other hand, superoxide dismutase, catalase and peroxidase activities were upregulated with increasing MCs concentrations. The bioaccumulation factor (BAF) of total MCs and MC-LR was highest at 6.50 and 5.00µgL−1, respectively, while for MC-RR, the highest BAF was recorded at 1.50µgL−1 concentration. The amount of total MCs, MC-LR and MC-RR bioacumulated in lettuce was highest at the highest exposure concentrations. However, at the lowest exposure concentration, there were no detectable levels of MC-LR, MC-RR and total MCs in lettuce. Thus, the bioaccumulation of MCs in lettuce varies according to the exposure concentration. In addition, the extent of physiological response of lettuce to the toxins relies on exposure concentrations.
[Display omitted]
•Lettuce was irrigated with microcystins (MCs) contaminated water.•Photosynthetic rates and other gas exchange parameters were significantly altered.•Protein content and antioxidant enzyme activities were significantly affected by MCs.•Variation of MCs bioaccumulation factor depended on exposure level and congener type.•Due to accumulated levels, irrigation with MCs contaminated water poses a health risk.</description><subject>Agricultural Irrigation</subject><subject>Antioxidants - metabolism</subject><subject>Catalase - metabolism</subject><subject>Food contamination</subject><subject>Glutathione Transferase - metabolism</subject><subject>Human health</subject><subject>Humans</subject><subject>Lactuca - drug effects</subject><subject>Lactuca - growth & development</subject><subject>Lactuca - metabolism</subject><subject>Microcystins</subject><subject>Microcystins - metabolism</subject><subject>Microcystins - toxicity</subject><subject>Microcystis - metabolism</subject><subject>Microcystis aeruginosa</subject><subject>Oxidative stress</subject><subject>Oxidative Stress - drug effects</subject><subject>Photosynthesis</subject><subject>Photosynthesis - drug effects</subject><subject>Plant Leaves - drug effects</subject><subject>Plant Leaves - growth & development</subject><subject>Plant Leaves - metabolism</subject><subject>Water Pollutants, Chemical - metabolism</subject><subject>Water Pollutants, Chemical - toxicity</subject><issn>0147-6513</issn><issn>1090-2414</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9UcFuGyEURFWrxkn7B1XFsYfuFlhgvTlUqqImrWSpPSRnhOERY3nBAdapf6DfHaxNeqyEQAzz3vBmEPpASUsJlV-2LZgI4dCyemsJawnlr9CCkoE0jFP-Gi0q0jdS0O4Mnee8JYR0RIi36IzJ5VCXWKC_KyhlMoB9Sv5eF7D40ZcNNjEUPfowI3VPl_j3JpaYj6FsoHiDwTkwJX_GOhQf_3iriz8ATpD3MWSosMVrH7Ux0zjt6mMMODo8epOiOebiw0nlHgKk_A69cXqX4f3zeYHurr_fXv1oVr9ufl59WzWmG0RphBHE6k4yR5nja617Zi0Y6iS3ayn4wFjHLCwlE3VW21PCtRy05M4IaXrXXaBPc999ig8T5KJGnw3sdjpAnLKiS8IYEV2_rFQ-U-t3c07g1D75UaejokSdElBbNSegTgkowlT1u5Z9fFaY1iPYf0UvllfC15kAdc6Dh6Sy8RAMWJ-qn8pG_3-FJ09RnUA</recordid><startdate>201606</startdate><enddate>201606</enddate><creator>Bittencourt-Oliveira, Maria do Carmo</creator><creator>Cordeiro-Araújo, Micheline Kézia</creator><creator>Chia, Mathias Ahii</creator><creator>Arruda-Neto, João Dias de Toledo</creator><creator>Oliveira, Ênio Tiago de</creator><creator>Santos, Flávio dos</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QH</scope><scope>7TV</scope><scope>7U7</scope><scope>7UA</scope><scope>C1K</scope><scope>F1W</scope><scope>H97</scope><scope>L.G</scope><scope>M7N</scope></search><sort><creationdate>201606</creationdate><title>Lettuce irrigated with contaminated water: Photosynthetic effects, antioxidative response and bioaccumulation of microcystin congeners</title><author>Bittencourt-Oliveira, Maria do Carmo ; 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In addition, MCs cause oxidative stress and negatively influence photosynthetic activities in plants. The present study was aimed at investigating the effect of MCs on photosynthetic parameters and antioxidative response of lettuce. Furthermore, the bioaccumulation factor (BAF) of total MCs, MC-LR and MC-RR in the vegetable after irrigation with contaminated water was determined. Lettuce crops were irrigated for 15 days with water containing cyanobacterial crude extracts (Microcystis aeruginosa) with MC-LR (0.0, 0.5, 2.0, 5.0 and 10.0µgL−1), MC-RR (0.0, 0.15, 0.5, 1.5 and 3.0µgL−1) and total MCs (0.0, 0.65, 2.5, 6.5 and 13.0µgL−1). Increased net photosynthetic rate, stomatal conductance, leaf tissue transpiration and intercellular CO2 concentration were recorded in lettuce exposed to different MCs concentrations. Antioxidant response showed that glutathione S-transferase activity was down-regulated in the presence of MCs. On the other hand, superoxide dismutase, catalase and peroxidase activities were upregulated with increasing MCs concentrations. The bioaccumulation factor (BAF) of total MCs and MC-LR was highest at 6.50 and 5.00µgL−1, respectively, while for MC-RR, the highest BAF was recorded at 1.50µgL−1 concentration. The amount of total MCs, MC-LR and MC-RR bioacumulated in lettuce was highest at the highest exposure concentrations. However, at the lowest exposure concentration, there were no detectable levels of MC-LR, MC-RR and total MCs in lettuce. Thus, the bioaccumulation of MCs in lettuce varies according to the exposure concentration. In addition, the extent of physiological response of lettuce to the toxins relies on exposure concentrations.
[Display omitted]
•Lettuce was irrigated with microcystins (MCs) contaminated water.•Photosynthetic rates and other gas exchange parameters were significantly altered.•Protein content and antioxidant enzyme activities were significantly affected by MCs.•Variation of MCs bioaccumulation factor depended on exposure level and congener type.•Due to accumulated levels, irrigation with MCs contaminated water poses a health risk.</abstract><cop>Netherlands</cop><pub>Elsevier Inc</pub><pmid>26896895</pmid><doi>10.1016/j.ecoenv.2016.02.014</doi><tpages>8</tpages></addata></record> |
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| ispartof | Ecotoxicology and environmental safety, 2016-06, Vol.128, p.83-90 |
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| subjects | Agricultural Irrigation Antioxidants - metabolism Catalase - metabolism Food contamination Glutathione Transferase - metabolism Human health Humans Lactuca - drug effects Lactuca - growth & development Lactuca - metabolism Microcystins Microcystins - metabolism Microcystins - toxicity Microcystis - metabolism Microcystis aeruginosa Oxidative stress Oxidative Stress - drug effects Photosynthesis Photosynthesis - drug effects Plant Leaves - drug effects Plant Leaves - growth & development Plant Leaves - metabolism Water Pollutants, Chemical - metabolism Water Pollutants, Chemical - toxicity |
| title | Lettuce irrigated with contaminated water: Photosynthetic effects, antioxidative response and bioaccumulation of microcystin congeners |
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