A hot water extract of Aralia cordata activates bone marrow-derived macrophages via a myeloid differentiation protein 88-dependent pathway and protects mice from bacterial infection
ABSTRACT In traditional Asian medicine, Aralia cordata (AC) is a known as a pain reliever and anti‐inflammatory drug. Although several of its biological activities have been reported, the immunomodulatory effects of a hot water extract of AC (HAC) have not yet been described. The aim of this study w...
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| Veröffentlicht in: | Microbiology and immunology 2016-05, Vol.60 (5), p.343-355 |
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| creator | Seo, Dong-Won Cho, Yong-Il Gu, Suna Kim, Da-Hee Park, Jung-Hee Yi, Young-Joo Lee, Sang-Myeong |
| description | ABSTRACT
In traditional Asian medicine, Aralia cordata (AC) is a known as a pain reliever and anti‐inflammatory drug. Although several of its biological activities have been reported, the immunomodulatory effects of a hot water extract of AC (HAC) have not yet been described. The aim of this study was to investigate whether HAC modulates the activation of macrophages, which play important roles in innate immune responses against microbial pathogens, and if so, to determine the molecular mechanisms by which HAC mediates this process. It was found that HAC activates bone marrow‐derived macrophages (BMDM) and increases amounts of nitric oxide and proinflammatory cytokines in a dose‐dependent manner. In addition, HAC was found to induce phosphorylation of NF‐κB and mitogen‐activated protein kinases (MAPKs), including c‐Jun N‐terminal kinases, extracellular signal‐regulated kinases and p38. Interestingly, these effects were absent in BMDM prepared from myeloid differentiation protein 88‐knockout mice. Polysaccharides from HAC exerted stronger immunostimulatory effects than HAC itself. Furthermore, orally administered HAC clearly enhanced clearance of the intracellular pathogen Listeria monocytogenes by boosting innate immune responses. These results demonstrate that HAC exerts immunostimulatory effects through the TLR/MyD88 and NF‐κB/MAPK signal transduction pathways. |
| doi_str_mv | 10.1111/1348-0421.12376 |
| format | Article |
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In traditional Asian medicine, Aralia cordata (AC) is a known as a pain reliever and anti‐inflammatory drug. Although several of its biological activities have been reported, the immunomodulatory effects of a hot water extract of AC (HAC) have not yet been described. The aim of this study was to investigate whether HAC modulates the activation of macrophages, which play important roles in innate immune responses against microbial pathogens, and if so, to determine the molecular mechanisms by which HAC mediates this process. It was found that HAC activates bone marrow‐derived macrophages (BMDM) and increases amounts of nitric oxide and proinflammatory cytokines in a dose‐dependent manner. In addition, HAC was found to induce phosphorylation of NF‐κB and mitogen‐activated protein kinases (MAPKs), including c‐Jun N‐terminal kinases, extracellular signal‐regulated kinases and p38. Interestingly, these effects were absent in BMDM prepared from myeloid differentiation protein 88‐knockout mice. Polysaccharides from HAC exerted stronger immunostimulatory effects than HAC itself. Furthermore, orally administered HAC clearly enhanced clearance of the intracellular pathogen Listeria monocytogenes by boosting innate immune responses. These results demonstrate that HAC exerts immunostimulatory effects through the TLR/MyD88 and NF‐κB/MAPK signal transduction pathways.</description><identifier>ISSN: 0385-5600</identifier><identifier>EISSN: 1348-0421</identifier><identifier>DOI: 10.1111/1348-0421.12376</identifier><identifier>PMID: 26989992</identifier><language>eng</language><publisher>Australia: Blackwell Publishing Ltd</publisher><subject>Animals ; Aralia ; Aralia - chemistry ; Aralia cordata ; Bacteria ; Bone marrow ; Immunity, Innate ; Immunologic Factors - isolation & purification ; Immunologic Factors - pharmacology ; immunostimulatory effect ; Kinases ; Listeria monocytogenes ; Listeria monocytogenes - immunology ; Listeriosis - immunology ; Listeriosis - prevention & control ; macrophages ; Macrophages - drug effects ; Macrophages - immunology ; Mice, Knockout ; Mitogen-Activated Protein Kinases - metabolism ; Myeloid Differentiation Factor 88 - deficiency ; Myeloid Differentiation Factor 88 - metabolism ; myeloid differentiation protein 88 ; NF-kappa B - metabolism ; Phosphorylation ; Plant Extracts - isolation & purification ; Plant Extracts - pharmacology ; Protein Processing, Post-Translational ; Proteins ; Rodents ; Signal transduction</subject><ispartof>Microbiology and immunology, 2016-05, Vol.60 (5), p.343-355</ispartof><rights>2016 The Societies and John Wiley & Sons Australia, Ltd</rights><rights>2016 The Societies and John Wiley & Sons Australia, Ltd.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4936-6b9ac3d976f42fd3eb78d66b31bca8b764068254f33e86a3706d36fd05533693</citedby><cites>FETCH-LOGICAL-c4936-6b9ac3d976f42fd3eb78d66b31bca8b764068254f33e86a3706d36fd05533693</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2F1348-0421.12376$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2F1348-0421.12376$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,1433,27924,27925,45574,45575,46409,46833</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26989992$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Seo, Dong-Won</creatorcontrib><creatorcontrib>Cho, Yong-Il</creatorcontrib><creatorcontrib>Gu, Suna</creatorcontrib><creatorcontrib>Kim, Da-Hee</creatorcontrib><creatorcontrib>Park, Jung-Hee</creatorcontrib><creatorcontrib>Yi, Young-Joo</creatorcontrib><creatorcontrib>Lee, Sang-Myeong</creatorcontrib><title>A hot water extract of Aralia cordata activates bone marrow-derived macrophages via a myeloid differentiation protein 88-dependent pathway and protects mice from bacterial infection</title><title>Microbiology and immunology</title><addtitle>Microbiol Immunol</addtitle><description>ABSTRACT
In traditional Asian medicine, Aralia cordata (AC) is a known as a pain reliever and anti‐inflammatory drug. Although several of its biological activities have been reported, the immunomodulatory effects of a hot water extract of AC (HAC) have not yet been described. The aim of this study was to investigate whether HAC modulates the activation of macrophages, which play important roles in innate immune responses against microbial pathogens, and if so, to determine the molecular mechanisms by which HAC mediates this process. It was found that HAC activates bone marrow‐derived macrophages (BMDM) and increases amounts of nitric oxide and proinflammatory cytokines in a dose‐dependent manner. In addition, HAC was found to induce phosphorylation of NF‐κB and mitogen‐activated protein kinases (MAPKs), including c‐Jun N‐terminal kinases, extracellular signal‐regulated kinases and p38. Interestingly, these effects were absent in BMDM prepared from myeloid differentiation protein 88‐knockout mice. Polysaccharides from HAC exerted stronger immunostimulatory effects than HAC itself. Furthermore, orally administered HAC clearly enhanced clearance of the intracellular pathogen Listeria monocytogenes by boosting innate immune responses. These results demonstrate that HAC exerts immunostimulatory effects through the TLR/MyD88 and NF‐κB/MAPK signal transduction pathways.</description><subject>Animals</subject><subject>Aralia</subject><subject>Aralia - chemistry</subject><subject>Aralia cordata</subject><subject>Bacteria</subject><subject>Bone marrow</subject><subject>Immunity, Innate</subject><subject>Immunologic Factors - isolation & purification</subject><subject>Immunologic Factors - pharmacology</subject><subject>immunostimulatory effect</subject><subject>Kinases</subject><subject>Listeria monocytogenes</subject><subject>Listeria monocytogenes - immunology</subject><subject>Listeriosis - immunology</subject><subject>Listeriosis - prevention & control</subject><subject>macrophages</subject><subject>Macrophages - drug effects</subject><subject>Macrophages - immunology</subject><subject>Mice, Knockout</subject><subject>Mitogen-Activated Protein Kinases - metabolism</subject><subject>Myeloid Differentiation Factor 88 - deficiency</subject><subject>Myeloid Differentiation Factor 88 - metabolism</subject><subject>myeloid differentiation protein 88</subject><subject>NF-kappa B - metabolism</subject><subject>Phosphorylation</subject><subject>Plant Extracts - isolation & purification</subject><subject>Plant Extracts - pharmacology</subject><subject>Protein Processing, Post-Translational</subject><subject>Proteins</subject><subject>Rodents</subject><subject>Signal transduction</subject><issn>0385-5600</issn><issn>1348-0421</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1uEzEUhUcIRENhzQ5ZYsNmWns8459lFJVSKYUiVXRpecY2cZkZB9tJmgfj_bhh2izY1BvLvt85176nKN4TfEZgnRNaixLXFTkjFeXsRTE73rwsZpiKpmwYxifFm5TuMa54JerXxUnFpJBSVrPizxytQkY7nW1E9iFH3WUUHJpH3XuNuhCNzhrBrd8Ck1AbRosGHWPYlcZGv7UGjl0M65X-CfUtqDQa9rYP3iDjnbPRjtnr7MOI1jFk60ckBIjXdjRQQmudVzu9R3o0E9DlhAbfWeRiGFALzaGR7pEfHdTA523xyuk-2XeP-2lx-_nidvGlXH67vFrMl2VXS8pK1krdUSM5c3XlDLUtF4axlpK206LlrMZMVE3tKLWCacoxM5Q5g5uGUibpafFpsoVX_d7YlNXgU2f7Xo82bJIiAlcVxkSS51EuYfyEUg7ox__Q-7CJI_wDKCFpXVNJgTqfKBhtStE6tY4e5r5XBKtD9uqQtDokrf5lD4oPj76bdrDmyD-FDUAzATvf2_1zfur66vrJuJx0PmX7cNTp-EsxTnmj7r5eqsUPLG7uvt-oJf0LzZnJ_Q</recordid><startdate>201605</startdate><enddate>201605</enddate><creator>Seo, Dong-Won</creator><creator>Cho, Yong-Il</creator><creator>Gu, Suna</creator><creator>Kim, Da-Hee</creator><creator>Park, Jung-Hee</creator><creator>Yi, Young-Joo</creator><creator>Lee, Sang-Myeong</creator><general>Blackwell Publishing Ltd</general><general>Wiley Subscription Services, Inc</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7U9</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>7X8</scope><scope>7QL</scope><scope>C1K</scope></search><sort><creationdate>201605</creationdate><title>A hot water extract of Aralia cordata activates bone marrow-derived macrophages via a myeloid differentiation protein 88-dependent pathway and protects mice from bacterial infection</title><author>Seo, Dong-Won ; Cho, Yong-Il ; Gu, Suna ; Kim, Da-Hee ; Park, Jung-Hee ; Yi, Young-Joo ; Lee, Sang-Myeong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4936-6b9ac3d976f42fd3eb78d66b31bca8b764068254f33e86a3706d36fd05533693</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Animals</topic><topic>Aralia</topic><topic>Aralia - chemistry</topic><topic>Aralia cordata</topic><topic>Bacteria</topic><topic>Bone marrow</topic><topic>Immunity, Innate</topic><topic>Immunologic Factors - isolation & purification</topic><topic>Immunologic Factors - pharmacology</topic><topic>immunostimulatory effect</topic><topic>Kinases</topic><topic>Listeria monocytogenes</topic><topic>Listeria monocytogenes - immunology</topic><topic>Listeriosis - immunology</topic><topic>Listeriosis - prevention & control</topic><topic>macrophages</topic><topic>Macrophages - drug effects</topic><topic>Macrophages - immunology</topic><topic>Mice, Knockout</topic><topic>Mitogen-Activated Protein Kinases - metabolism</topic><topic>Myeloid Differentiation Factor 88 - deficiency</topic><topic>Myeloid Differentiation Factor 88 - metabolism</topic><topic>myeloid differentiation protein 88</topic><topic>NF-kappa B - metabolism</topic><topic>Phosphorylation</topic><topic>Plant Extracts - isolation & purification</topic><topic>Plant Extracts - pharmacology</topic><topic>Protein Processing, Post-Translational</topic><topic>Proteins</topic><topic>Rodents</topic><topic>Signal transduction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Seo, Dong-Won</creatorcontrib><creatorcontrib>Cho, Yong-Il</creatorcontrib><creatorcontrib>Gu, Suna</creatorcontrib><creatorcontrib>Kim, Da-Hee</creatorcontrib><creatorcontrib>Park, Jung-Hee</creatorcontrib><creatorcontrib>Yi, Young-Joo</creatorcontrib><creatorcontrib>Lee, Sang-Myeong</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Microbiology and immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Seo, Dong-Won</au><au>Cho, Yong-Il</au><au>Gu, Suna</au><au>Kim, Da-Hee</au><au>Park, Jung-Hee</au><au>Yi, Young-Joo</au><au>Lee, Sang-Myeong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A hot water extract of Aralia cordata activates bone marrow-derived macrophages via a myeloid differentiation protein 88-dependent pathway and protects mice from bacterial infection</atitle><jtitle>Microbiology and immunology</jtitle><addtitle>Microbiol Immunol</addtitle><date>2016-05</date><risdate>2016</risdate><volume>60</volume><issue>5</issue><spage>343</spage><epage>355</epage><pages>343-355</pages><issn>0385-5600</issn><eissn>1348-0421</eissn><abstract>ABSTRACT
In traditional Asian medicine, Aralia cordata (AC) is a known as a pain reliever and anti‐inflammatory drug. Although several of its biological activities have been reported, the immunomodulatory effects of a hot water extract of AC (HAC) have not yet been described. The aim of this study was to investigate whether HAC modulates the activation of macrophages, which play important roles in innate immune responses against microbial pathogens, and if so, to determine the molecular mechanisms by which HAC mediates this process. It was found that HAC activates bone marrow‐derived macrophages (BMDM) and increases amounts of nitric oxide and proinflammatory cytokines in a dose‐dependent manner. In addition, HAC was found to induce phosphorylation of NF‐κB and mitogen‐activated protein kinases (MAPKs), including c‐Jun N‐terminal kinases, extracellular signal‐regulated kinases and p38. Interestingly, these effects were absent in BMDM prepared from myeloid differentiation protein 88‐knockout mice. Polysaccharides from HAC exerted stronger immunostimulatory effects than HAC itself. Furthermore, orally administered HAC clearly enhanced clearance of the intracellular pathogen Listeria monocytogenes by boosting innate immune responses. These results demonstrate that HAC exerts immunostimulatory effects through the TLR/MyD88 and NF‐κB/MAPK signal transduction pathways.</abstract><cop>Australia</cop><pub>Blackwell Publishing Ltd</pub><pmid>26989992</pmid><doi>10.1111/1348-0421.12376</doi><tpages>13</tpages></addata></record> |
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| subjects | Animals Aralia Aralia - chemistry Aralia cordata Bacteria Bone marrow Immunity, Innate Immunologic Factors - isolation & purification Immunologic Factors - pharmacology immunostimulatory effect Kinases Listeria monocytogenes Listeria monocytogenes - immunology Listeriosis - immunology Listeriosis - prevention & control macrophages Macrophages - drug effects Macrophages - immunology Mice, Knockout Mitogen-Activated Protein Kinases - metabolism Myeloid Differentiation Factor 88 - deficiency Myeloid Differentiation Factor 88 - metabolism myeloid differentiation protein 88 NF-kappa B - metabolism Phosphorylation Plant Extracts - isolation & purification Plant Extracts - pharmacology Protein Processing, Post-Translational Proteins Rodents Signal transduction |
| title | A hot water extract of Aralia cordata activates bone marrow-derived macrophages via a myeloid differentiation protein 88-dependent pathway and protects mice from bacterial infection |
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