Digestion of Chlorella Cells by Chlorovirus-encoded Polysaccharide Degrading Enzymes
Using a halo-forming assay with polysaccharide-degrading enzymes encoded by Chlorella virus (chlorovirus) CVK2, several free-living Chlorella strains, especially those belonging to C. vulgaris, were found to be sensitive to the viral enzymes. Among these sensitive strains, a taxonomically establishe...
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| Veröffentlicht in: | Microbes and Environments 2001, Vol.16(4), pp.206-212 |
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| container_title | Microbes and Environments |
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| creator | Chuchird, Niti Hiramatsu, Shingo Sugimoto, Ichiro Fujie, Makoto Usami, Shoji Yamada, Takashi |
| description | Using a halo-forming assay with polysaccharide-degrading enzymes encoded by Chlorella virus (chlorovirus) CVK2, several free-living Chlorella strains, especially those belonging to C. vulgaris, were found to be sensitive to the viral enzymes. Among these sensitive strains, a taxonomically established strain C. prototechoides 211-6 served as a new laboratory host for chloroviruses (NC64A-viruses). Many zoochlorella strains isolated from Paramecium were totally resistant to the enzymes. Attachment of CVK2 to the cells of both Chlorella strain NC64A and C. prototechoides 211-6 was markedly blocked by treatment of the cells with a mixture of the viral enzymes. The treatment with vAL-1 may destroy the virus receptor molecules. |
| doi_str_mv | 10.1264/jsme2.2001.206 |
| format | Article |
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| source | J-STAGE Free; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Chlorella virus chlorovirus halo-assay new laboratory host Paramecium Zoochlorella |
| title | Digestion of Chlorella Cells by Chlorovirus-encoded Polysaccharide Degrading Enzymes |
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