MiR-330-5p regulates tyrosinase and PDIA3 expression and suppresses cell proliferation and invasion in cutaneous malignant melanoma

Abstract Background Increasing evidences has suggested that miR-330-5p can function as a tumor suppressor in different types of cancers. However, the effects and the underlying mechanisms of miR-330-5p in the development of cutaneous malignant melanoma (CMM) remain largely unknown. The aim of the present study was to investigate the role of miR-330-5p in CMM and to determine the molecular mechanisms underlying its action. Materials and methods The expression level of miR-330-5p was detected in 26 cases of primary cutaneous malignant melanoma (PCMM) tissues and cell lines by real-time quantitative PCR. We also assessed whether overexpression of miR-330-5p influence in vitro cell proliferation, invasion and migration. Western blotting analysis was used to detect the influence of miR-330-5p on the targets, and Pearson analysis was used to calculate the correlation between the expression of targets gene and miR-330-5p in CMM tissues. Results Our study showed that miR-330-5p was downregulated in CMM tissues (p=0.010) and cell lines (p < 0.05), and patients with high mitotic activity showed lower miR-330-5p expression levels (p = 0.002). Enforced expression of miR-330-5p inhibit malignant CMM cells proliferation and migration, led to downregulation of the TYR and PDIA3 protein. Moreover, the expression level of miR-330-5p in CMM tissues showed inversely relationship with the expression level of TYR and PDIA3 protein. Conclusion In conclusion, our findings suggested that miR-330-5p represents a potential tumor suppressive-miRNA and plays an important role in CMM progression by suppressing TYR and PDIA3 expression.