Role of efflux pump inhibitor in decreasing antibiotic cross-resistance of Pseudomonas aeruginosa in a burn hospital in Iran
Multidrug resistance in Pseudomonas aeruginosa may be due to efflux pump overexpression. This study phenotypically examined the role of efflux pump inhibitors in decreasing antibiotic cross-resistance between beta-lactams, fluoroquinolones, and aminoglycosides in P. aeruginosa isolates from burn pat...
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| Veröffentlicht in: | Journal of infection in developing countries 2016-06, Vol.10 (6), p.600-604 |
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| creator | Talebi-Taher, Mahshid Majidpour, َAli Gholami, Abbas Rasouli-Kouhi, Samira Adabi, Maryam |
| description | Multidrug resistance in Pseudomonas aeruginosa may be due to efflux pump overexpression. This study phenotypically examined the role of efflux pump inhibitors in decreasing antibiotic cross-resistance between beta-lactams, fluoroquinolones, and aminoglycosides in P. aeruginosa isolates from burn patients in Iran.
A total of 91 phenotypically and genotypically confirmed P. aeruginosa samples were studied. Multidrug cross-resistance was determined using the disk diffusion method and minimum inhibitory concentration (MIC) test. The contribution of efflux pumps was determined by investigating MIC reduction assay to markers of beta-lactams, fluoroquinolones, and aminoglycosides in the absence and presence of an efflux pump inhibitor. All the isolates were also tested by polymerase chain reaction for the presence of mexA, mexC, and mexE efflux genes.
Of the isolates, 81 (89%) and 83 (91.2%) were multidrug resistant according to the disk diffusion and MIC method, respectively. Cross-resistance was observed in 67 (73.6%) and 68 (74.7%) of isolates according to the disk diffusion and MIC method, respectively. In the presence of the efflux pump inhibitor, twofold or higher MIC reduction to imipenem, cefepime, ciprofloxacin, and gentamicin was observed in 59, 65, 55, and 60 isolates, respectively. Except for two isolates that were negative for mexC, all isolates were positive for mexA, mexC, and mexE genes simultaneously.
Efflux pumps could cause different levels of resistance based on their expression in clinical isolates. Early detection of different efflux pumps in P. aeruginosa could allow the use of other antibiotics and efflux pump inhibitors in combination with antibiotic therapy. |
| doi_str_mv | 10.3855/jidc.7619 |
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A total of 91 phenotypically and genotypically confirmed P. aeruginosa samples were studied. Multidrug cross-resistance was determined using the disk diffusion method and minimum inhibitory concentration (MIC) test. The contribution of efflux pumps was determined by investigating MIC reduction assay to markers of beta-lactams, fluoroquinolones, and aminoglycosides in the absence and presence of an efflux pump inhibitor. All the isolates were also tested by polymerase chain reaction for the presence of mexA, mexC, and mexE efflux genes.
Of the isolates, 81 (89%) and 83 (91.2%) were multidrug resistant according to the disk diffusion and MIC method, respectively. Cross-resistance was observed in 67 (73.6%) and 68 (74.7%) of isolates according to the disk diffusion and MIC method, respectively. In the presence of the efflux pump inhibitor, twofold or higher MIC reduction to imipenem, cefepime, ciprofloxacin, and gentamicin was observed in 59, 65, 55, and 60 isolates, respectively. Except for two isolates that were negative for mexC, all isolates were positive for mexA, mexC, and mexE genes simultaneously.
Efflux pumps could cause different levels of resistance based on their expression in clinical isolates. Early detection of different efflux pumps in P. aeruginosa could allow the use of other antibiotics and efflux pump inhibitors in combination with antibiotic therapy.</description><identifier>ISSN: 1972-2680</identifier><identifier>ISSN: 2036-6590</identifier><identifier>EISSN: 1972-2680</identifier><identifier>DOI: 10.3855/jidc.7619</identifier><identifier>PMID: 27367008</identifier><language>eng</language><publisher>Italy: Journal of Infection in Developing Countries</publisher><subject>Aminoglycosides - pharmacology ; Anti-Bacterial Agents - pharmacology ; Antibiotics ; beta-Lactams - pharmacology ; Biological Transport, Active - drug effects ; Burns - complications ; Drug Resistance, Bacterial - drug effects ; Fluoroquinolones - pharmacology ; Genes, Bacterial ; Hospitals ; Humans ; Iran ; Microbial Sensitivity Tests ; Penicillin ; Polymerase Chain Reaction ; Pseudomonas aeruginosa - drug effects ; Pseudomonas aeruginosa - isolation & purification ; Pseudomonas Infections - microbiology</subject><ispartof>Journal of infection in developing countries, 2016-06, Vol.10 (6), p.600-604</ispartof><rights>2016. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c379t-bc7477a7e1024c2fae05da3b4d9cb8655bfd50ddfcff6828e496ac133a40d57b3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27367008$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Talebi-Taher, Mahshid</creatorcontrib><creatorcontrib>Majidpour, َAli</creatorcontrib><creatorcontrib>Gholami, Abbas</creatorcontrib><creatorcontrib>Rasouli-Kouhi, Samira</creatorcontrib><creatorcontrib>Adabi, Maryam</creatorcontrib><title>Role of efflux pump inhibitor in decreasing antibiotic cross-resistance of Pseudomonas aeruginosa in a burn hospital in Iran</title><title>Journal of infection in developing countries</title><addtitle>J Infect Dev Ctries</addtitle><description>Multidrug resistance in Pseudomonas aeruginosa may be due to efflux pump overexpression. This study phenotypically examined the role of efflux pump inhibitors in decreasing antibiotic cross-resistance between beta-lactams, fluoroquinolones, and aminoglycosides in P. aeruginosa isolates from burn patients in Iran.
A total of 91 phenotypically and genotypically confirmed P. aeruginosa samples were studied. Multidrug cross-resistance was determined using the disk diffusion method and minimum inhibitory concentration (MIC) test. The contribution of efflux pumps was determined by investigating MIC reduction assay to markers of beta-lactams, fluoroquinolones, and aminoglycosides in the absence and presence of an efflux pump inhibitor. All the isolates were also tested by polymerase chain reaction for the presence of mexA, mexC, and mexE efflux genes.
Of the isolates, 81 (89%) and 83 (91.2%) were multidrug resistant according to the disk diffusion and MIC method, respectively. Cross-resistance was observed in 67 (73.6%) and 68 (74.7%) of isolates according to the disk diffusion and MIC method, respectively. In the presence of the efflux pump inhibitor, twofold or higher MIC reduction to imipenem, cefepime, ciprofloxacin, and gentamicin was observed in 59, 65, 55, and 60 isolates, respectively. Except for two isolates that were negative for mexC, all isolates were positive for mexA, mexC, and mexE genes simultaneously.
Efflux pumps could cause different levels of resistance based on their expression in clinical isolates. Early detection of different efflux pumps in P. aeruginosa could allow the use of other antibiotics and efflux pump inhibitors in combination with antibiotic therapy.</description><subject>Aminoglycosides - pharmacology</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Antibiotics</subject><subject>beta-Lactams - pharmacology</subject><subject>Biological Transport, Active - drug effects</subject><subject>Burns - complications</subject><subject>Drug Resistance, Bacterial - drug effects</subject><subject>Fluoroquinolones - pharmacology</subject><subject>Genes, Bacterial</subject><subject>Hospitals</subject><subject>Humans</subject><subject>Iran</subject><subject>Microbial Sensitivity Tests</subject><subject>Penicillin</subject><subject>Polymerase Chain Reaction</subject><subject>Pseudomonas aeruginosa - drug effects</subject><subject>Pseudomonas aeruginosa - isolation & purification</subject><subject>Pseudomonas Infections - microbiology</subject><issn>1972-2680</issn><issn>2036-6590</issn><issn>1972-2680</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><recordid>eNpdkUtLxDAUhYMozvhY-Ack4EYX1aRpmnYp4gsERXRdbvMYM7RJTVpQ8Mfbzowiru7h8HE43IPQESXnrOD8YmmVPBc5LbfQnJYiTdK8INt_9AztxbgkhJeM0100SwXLBSHFHH09-0Zjb7A2phk-cDe0Hbbuzda292FUWGkZNETrFhhcP_q-txLL4GNMgo429uDkKuIp6kH51juIGHQYFtb5CFMG4HoIDr_52Nkemsm6D-AO0I6BJurDzd1HrzfXL1d3ycPj7f3V5UMimSj7pJYiEwKEpiTNZGpAE66A1ZkqZV3knNdGcaKUkcbkRVrorMxBUsYgI4qLmu2j03VuF_z7oGNftTZK3TTgtB9iRQtCM0aFKEf05B-69GP1sV2V8pxQmpeUjtTZmlq9IWhTdcG2ED4rSqppkmqapJomGdnjTeJQt1r9kj8bsG8r8Yk2</recordid><startdate>20160630</startdate><enddate>20160630</enddate><creator>Talebi-Taher, Mahshid</creator><creator>Majidpour, َAli</creator><creator>Gholami, Abbas</creator><creator>Rasouli-Kouhi, Samira</creator><creator>Adabi, Maryam</creator><general>Journal of Infection in Developing Countries</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8C1</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>20160630</creationdate><title>Role of efflux pump inhibitor in decreasing antibiotic cross-resistance of Pseudomonas aeruginosa in a burn hospital in Iran</title><author>Talebi-Taher, Mahshid ; 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This study phenotypically examined the role of efflux pump inhibitors in decreasing antibiotic cross-resistance between beta-lactams, fluoroquinolones, and aminoglycosides in P. aeruginosa isolates from burn patients in Iran.
A total of 91 phenotypically and genotypically confirmed P. aeruginosa samples were studied. Multidrug cross-resistance was determined using the disk diffusion method and minimum inhibitory concentration (MIC) test. The contribution of efflux pumps was determined by investigating MIC reduction assay to markers of beta-lactams, fluoroquinolones, and aminoglycosides in the absence and presence of an efflux pump inhibitor. All the isolates were also tested by polymerase chain reaction for the presence of mexA, mexC, and mexE efflux genes.
Of the isolates, 81 (89%) and 83 (91.2%) were multidrug resistant according to the disk diffusion and MIC method, respectively. Cross-resistance was observed in 67 (73.6%) and 68 (74.7%) of isolates according to the disk diffusion and MIC method, respectively. In the presence of the efflux pump inhibitor, twofold or higher MIC reduction to imipenem, cefepime, ciprofloxacin, and gentamicin was observed in 59, 65, 55, and 60 isolates, respectively. Except for two isolates that were negative for mexC, all isolates were positive for mexA, mexC, and mexE genes simultaneously.
Efflux pumps could cause different levels of resistance based on their expression in clinical isolates. Early detection of different efflux pumps in P. aeruginosa could allow the use of other antibiotics and efflux pump inhibitors in combination with antibiotic therapy.</abstract><cop>Italy</cop><pub>Journal of Infection in Developing Countries</pub><pmid>27367008</pmid><doi>10.3855/jidc.7619</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Aminoglycosides - pharmacology Anti-Bacterial Agents - pharmacology Antibiotics beta-Lactams - pharmacology Biological Transport, Active - drug effects Burns - complications Drug Resistance, Bacterial - drug effects Fluoroquinolones - pharmacology Genes, Bacterial Hospitals Humans Iran Microbial Sensitivity Tests Penicillin Polymerase Chain Reaction Pseudomonas aeruginosa - drug effects Pseudomonas aeruginosa - isolation & purification Pseudomonas Infections - microbiology |
| title | Role of efflux pump inhibitor in decreasing antibiotic cross-resistance of Pseudomonas aeruginosa in a burn hospital in Iran |
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