Roles of two-component system AfsQ1/Q2 in regulating biosynthesis of the yellow-pigmented coelimycin P2 in Streptomyces coelicolor
We previously demonstrated that in Streptomyces coelicolor two-component system AfsQ1/Q2 activates the production of the yellow-colored coelimycin P2 (also named as yCPK) on glutamate-supplemented minimal medium, and the response regulator AfsQ1 could specifically bind to the intergenic region betwe...
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| Veröffentlicht in: | FEMS microbiology letters 2016-08, Vol.363 (15), p.fnw160 |
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| creator | Chen, Shuangshuang Zheng, Guosong Zhu, Hong He, Huiqi Chen, Lei Zhang, Weiwen Jiang, Weihong Lu, Yinhua |
| description | We previously demonstrated that in Streptomyces coelicolor two-component system AfsQ1/Q2 activates the production of the yellow-colored coelimycin P2 (also named as yCPK) on glutamate-supplemented minimal medium, and the response regulator AfsQ1 could specifically bind to the intergenic region between two structural genes, cpkA and cpkD. Here, a more in-depth investigation was performed to elucidate the mechanism underlying the role of AfsQ1/Q2 in regulating coelimycin P2 biosynthesis. Deletion of afsQ1/Q2 resulted in markedly decreased expression of the whole coelimycin P2 biosynthetic gene cluster. Electrophoretic mobility shift assays revealed that AfsQ1 bound only to the target site identified previously, but not to any other promoters in the gene cluster. Mutations of AfsQ1-binding motif only resulted in drastically reduced transcription of the cpkA/B/C operon (encoding three type I polyketide synthases) and intriguingly, led to enhanced expression of some coelimcyin P2 genes, particularly accA1 and scF. These results suggested the direct role of AfsQ1/Q2 in regulating coelimycin production, which is directly mediated by the structural genes, but not the cluster-situated regulatory genes, and also implied that other unknown mechanisms may be involved in AfsQ1/Q2-mediated regulation of coelimycin P2 biosynthesis.
This study revealed a direct role of AfsQ1/Q2 in regulating coelimycin production, which is directly mediated by the structural genes, but not the cluster-situated regulatory genes. |
| doi_str_mv | 10.1093/femsle/fnw160 |
| format | Article |
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This study revealed a direct role of AfsQ1/Q2 in regulating coelimycin production, which is directly mediated by the structural genes, but not the cluster-situated regulatory genes.</description><identifier>ISSN: 1574-6968</identifier><identifier>ISSN: 0378-1097</identifier><identifier>EISSN: 1574-6968</identifier><identifier>DOI: 10.1093/femsle/fnw160</identifier><identifier>PMID: 27313101</identifier><language>eng</language><publisher>England: Oxford University Press</publisher><subject>Anti-Bacterial Agents - biosynthesis ; Bacterial Proteins - genetics ; Biosynthesis ; Electrophoretic mobility ; Electrophoretic Mobility Shift Assay ; Gene Expression Regulation, Bacterial ; Genes ; Genes, Regulator ; Microbiology ; Mutation ; Operon ; Polyketide Synthases - genetics ; Promoter Regions, Genetic ; Streptomyces coelicolor ; Streptomyces coelicolor - genetics ; Streptomyces coelicolor - metabolism ; Target recognition ; Transcription</subject><ispartof>FEMS microbiology letters, 2016-08, Vol.363 (15), p.fnw160</ispartof><rights>FEMS 2016. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com 2016</rights><rights>FEMS 2016. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.</rights><rights>FEMS 2016. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c424t-a4dba1f08fb13f514734935284b0a86efeeb5def8f7a2a681d39ed5cf6a9cf3d3</citedby><cites>FETCH-LOGICAL-c424t-a4dba1f08fb13f514734935284b0a86efeeb5def8f7a2a681d39ed5cf6a9cf3d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,1584,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27313101$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Clarke, David</contributor><creatorcontrib>Chen, Shuangshuang</creatorcontrib><creatorcontrib>Zheng, Guosong</creatorcontrib><creatorcontrib>Zhu, Hong</creatorcontrib><creatorcontrib>He, Huiqi</creatorcontrib><creatorcontrib>Chen, Lei</creatorcontrib><creatorcontrib>Zhang, Weiwen</creatorcontrib><creatorcontrib>Jiang, Weihong</creatorcontrib><creatorcontrib>Lu, Yinhua</creatorcontrib><title>Roles of two-component system AfsQ1/Q2 in regulating biosynthesis of the yellow-pigmented coelimycin P2 in Streptomyces coelicolor</title><title>FEMS microbiology letters</title><addtitle>FEMS Microbiol Lett</addtitle><description>We previously demonstrated that in Streptomyces coelicolor two-component system AfsQ1/Q2 activates the production of the yellow-colored coelimycin P2 (also named as yCPK) on glutamate-supplemented minimal medium, and the response regulator AfsQ1 could specifically bind to the intergenic region between two structural genes, cpkA and cpkD. Here, a more in-depth investigation was performed to elucidate the mechanism underlying the role of AfsQ1/Q2 in regulating coelimycin P2 biosynthesis. Deletion of afsQ1/Q2 resulted in markedly decreased expression of the whole coelimycin P2 biosynthetic gene cluster. Electrophoretic mobility shift assays revealed that AfsQ1 bound only to the target site identified previously, but not to any other promoters in the gene cluster. Mutations of AfsQ1-binding motif only resulted in drastically reduced transcription of the cpkA/B/C operon (encoding three type I polyketide synthases) and intriguingly, led to enhanced expression of some coelimcyin P2 genes, particularly accA1 and scF. These results suggested the direct role of AfsQ1/Q2 in regulating coelimycin production, which is directly mediated by the structural genes, but not the cluster-situated regulatory genes, and also implied that other unknown mechanisms may be involved in AfsQ1/Q2-mediated regulation of coelimycin P2 biosynthesis.
This study revealed a direct role of AfsQ1/Q2 in regulating coelimycin production, which is directly mediated by the structural genes, but not the cluster-situated regulatory genes.</description><subject>Anti-Bacterial Agents - biosynthesis</subject><subject>Bacterial Proteins - genetics</subject><subject>Biosynthesis</subject><subject>Electrophoretic mobility</subject><subject>Electrophoretic Mobility Shift Assay</subject><subject>Gene Expression Regulation, Bacterial</subject><subject>Genes</subject><subject>Genes, Regulator</subject><subject>Microbiology</subject><subject>Mutation</subject><subject>Operon</subject><subject>Polyketide Synthases - genetics</subject><subject>Promoter Regions, Genetic</subject><subject>Streptomyces coelicolor</subject><subject>Streptomyces coelicolor - genetics</subject><subject>Streptomyces coelicolor - metabolism</subject><subject>Target recognition</subject><subject>Transcription</subject><issn>1574-6968</issn><issn>0378-1097</issn><issn>1574-6968</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqFkTlPxDAQhS0EYrlKWmSJhiasr2STcoW4JCTuOnKc8W5WSRxsR6u0_HIM4RINlUeeb948zUPokJJTSjI-1dC4Gqa6XdOEbKAdGs9ElGRJuvmrnqBd51aEEMFIso0mbMYpp4TuoNcHU4PDRmO_NpEyTWdaaD12g_PQ4Ll293R6z3DVYguLvpa-ahe4qIwbWr8EV42zS8AD1LVZR121aIIAlFgZqKtmUGH07kPg0VvovAlfYeNHV5na2H20pWXt4ODz3UPPF-dPZ1fRze3l9dn8JlKCCR9JURaSapLqgnIdUzHjIuMxS0VBZJqABijiEnSqZ5LJJKUlz6CMlU5kpjQv-R46GXU7a156cD5vKqeCa9mC6V1OU0LDpixlAT3-g65Mb9vgLmciHI4JkolARSOlrHHOgs47WzXSDjkl-Xs4-RhOPoYT-KNP1b5ooPymv9L4cWj67h-tN5JDnQE</recordid><startdate>20160801</startdate><enddate>20160801</enddate><creator>Chen, Shuangshuang</creator><creator>Zheng, Guosong</creator><creator>Zhu, Hong</creator><creator>He, Huiqi</creator><creator>Chen, Lei</creator><creator>Zhang, Weiwen</creator><creator>Jiang, Weihong</creator><creator>Lu, Yinhua</creator><general>Oxford University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20160801</creationdate><title>Roles of two-component system AfsQ1/Q2 in regulating biosynthesis of the yellow-pigmented coelimycin P2 in Streptomyces coelicolor</title><author>Chen, Shuangshuang ; Zheng, Guosong ; Zhu, Hong ; He, Huiqi ; Chen, Lei ; Zhang, Weiwen ; Jiang, Weihong ; Lu, Yinhua</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c424t-a4dba1f08fb13f514734935284b0a86efeeb5def8f7a2a681d39ed5cf6a9cf3d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Anti-Bacterial Agents - biosynthesis</topic><topic>Bacterial Proteins - genetics</topic><topic>Biosynthesis</topic><topic>Electrophoretic mobility</topic><topic>Electrophoretic Mobility Shift Assay</topic><topic>Gene Expression Regulation, Bacterial</topic><topic>Genes</topic><topic>Genes, Regulator</topic><topic>Microbiology</topic><topic>Mutation</topic><topic>Operon</topic><topic>Polyketide Synthases - genetics</topic><topic>Promoter Regions, Genetic</topic><topic>Streptomyces coelicolor</topic><topic>Streptomyces coelicolor - genetics</topic><topic>Streptomyces coelicolor - metabolism</topic><topic>Target recognition</topic><topic>Transcription</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chen, Shuangshuang</creatorcontrib><creatorcontrib>Zheng, Guosong</creatorcontrib><creatorcontrib>Zhu, Hong</creatorcontrib><creatorcontrib>He, Huiqi</creatorcontrib><creatorcontrib>Chen, Lei</creatorcontrib><creatorcontrib>Zhang, Weiwen</creatorcontrib><creatorcontrib>Jiang, Weihong</creatorcontrib><creatorcontrib>Lu, Yinhua</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>FEMS microbiology letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chen, Shuangshuang</au><au>Zheng, Guosong</au><au>Zhu, Hong</au><au>He, Huiqi</au><au>Chen, Lei</au><au>Zhang, Weiwen</au><au>Jiang, Weihong</au><au>Lu, Yinhua</au><au>Clarke, David</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Roles of two-component system AfsQ1/Q2 in regulating biosynthesis of the yellow-pigmented coelimycin P2 in Streptomyces coelicolor</atitle><jtitle>FEMS microbiology letters</jtitle><addtitle>FEMS Microbiol Lett</addtitle><date>2016-08-01</date><risdate>2016</risdate><volume>363</volume><issue>15</issue><spage>fnw160</spage><pages>fnw160-</pages><issn>1574-6968</issn><issn>0378-1097</issn><eissn>1574-6968</eissn><abstract>We previously demonstrated that in Streptomyces coelicolor two-component system AfsQ1/Q2 activates the production of the yellow-colored coelimycin P2 (also named as yCPK) on glutamate-supplemented minimal medium, and the response regulator AfsQ1 could specifically bind to the intergenic region between two structural genes, cpkA and cpkD. Here, a more in-depth investigation was performed to elucidate the mechanism underlying the role of AfsQ1/Q2 in regulating coelimycin P2 biosynthesis. Deletion of afsQ1/Q2 resulted in markedly decreased expression of the whole coelimycin P2 biosynthetic gene cluster. Electrophoretic mobility shift assays revealed that AfsQ1 bound only to the target site identified previously, but not to any other promoters in the gene cluster. Mutations of AfsQ1-binding motif only resulted in drastically reduced transcription of the cpkA/B/C operon (encoding three type I polyketide synthases) and intriguingly, led to enhanced expression of some coelimcyin P2 genes, particularly accA1 and scF. These results suggested the direct role of AfsQ1/Q2 in regulating coelimycin production, which is directly mediated by the structural genes, but not the cluster-situated regulatory genes, and also implied that other unknown mechanisms may be involved in AfsQ1/Q2-mediated regulation of coelimycin P2 biosynthesis.
This study revealed a direct role of AfsQ1/Q2 in regulating coelimycin production, which is directly mediated by the structural genes, but not the cluster-situated regulatory genes.</abstract><cop>England</cop><pub>Oxford University Press</pub><pmid>27313101</pmid><doi>10.1093/femsle/fnw160</doi></addata></record> |
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| subjects | Anti-Bacterial Agents - biosynthesis Bacterial Proteins - genetics Biosynthesis Electrophoretic mobility Electrophoretic Mobility Shift Assay Gene Expression Regulation, Bacterial Genes Genes, Regulator Microbiology Mutation Operon Polyketide Synthases - genetics Promoter Regions, Genetic Streptomyces coelicolor Streptomyces coelicolor - genetics Streptomyces coelicolor - metabolism Target recognition Transcription |
| title | Roles of two-component system AfsQ1/Q2 in regulating biosynthesis of the yellow-pigmented coelimycin P2 in Streptomyces coelicolor |
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