Outer membrane protein C (OmpC) of Escherichia coli induces neurodegeneration in mice by acting as an amyloid
OBJECTIVES: Involvement of the outer membrane protein C (OmpC) of Escherichia coli in neurodegeneration was investigated using a mouse model. RESULTS: OmpC formed protease-resistant fibres that exhibited the diagnostic features of an amyloid. The spectral shift in the Congo Red and the thioflavin T...
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| Veröffentlicht in: | Biotechnology letters 2016-04, Vol.38 (4), p.689-700 |
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| creator | Joseph Sahaya Rajan, J Chinnappan Santiago, T Singaravel, R Ignacimuthu, S |
| description | OBJECTIVES: Involvement of the outer membrane protein C (OmpC) of Escherichia coli in neurodegeneration was investigated using a mouse model. RESULTS: OmpC formed protease-resistant fibres that exhibited the diagnostic features of an amyloid. The spectral shift in the Congo Red and the thioflavin T assays produced features similar to neurotoxic peptides. Intramuscular administration of OmpC in mice resulted in spongiform neurodegeneration of the brain through calcium-dependent apoptosis and also showed upregulation of apoptosis related genes. Immunolocalization of OmpC in brain demonstrated the direct involvement of the porin in neurodegeneration and formation of spongiform encephalopathy. CONCLUSION: We have demonstrated the ability of OmpC of E. coli to induce neurodegeneration in mice. |
| doi_str_mv | 10.1007/s10529-015-2025-8 |
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RESULTS: OmpC formed protease-resistant fibres that exhibited the diagnostic features of an amyloid. The spectral shift in the Congo Red and the thioflavin T assays produced features similar to neurotoxic peptides. Intramuscular administration of OmpC in mice resulted in spongiform neurodegeneration of the brain through calcium-dependent apoptosis and also showed upregulation of apoptosis related genes. Immunolocalization of OmpC in brain demonstrated the direct involvement of the porin in neurodegeneration and formation of spongiform encephalopathy. CONCLUSION: We have demonstrated the ability of OmpC of E. coli to induce neurodegeneration in mice.</description><identifier>ISSN: 0141-5492</identifier><identifier>EISSN: 1573-6776</identifier><identifier>DOI: 10.1007/s10529-015-2025-8</identifier><identifier>PMID: 26712371</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>amyloid ; animal models ; Animals ; Apoptosis ; Applied Microbiology ; Biochemistry ; Biomedical and Life Sciences ; Biotechnology ; Brain ; Brain - metabolism ; Brain - pathology ; Calcium - metabolism ; Disease Models, Animal ; E coli ; encephalopathy ; Escherichia coli ; Fibers ; Gene Expression Regulation ; genes ; Injections, Intramuscular ; intramuscular injection ; Life Sciences ; Membranes ; Mice ; Microbiology ; Neurodegeneration ; neurodegenerative diseases ; Neurodegenerative Diseases - etiology ; Neurodegenerative Diseases - metabolism ; Neurodegenerative Diseases - pathology ; neurotoxicity ; Original Research Paper ; outer membrane proteins ; Peptides ; porins ; Porins - administration & dosage ; Porins - toxicity ; Proteases ; Proteins ; Spectra ; Spongiform encephalopathies</subject><ispartof>Biotechnology letters, 2016-04, Vol.38 (4), p.689-700</ispartof><rights>Springer Science+Business Media Dordrecht 2015</rights><rights>Springer Science+Business Media Dordrecht 2016</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c569t-59bb29b48298c95e6a9006b7265cdcf7878a06cc828e916841625d58563a66b63</citedby><cites>FETCH-LOGICAL-c569t-59bb29b48298c95e6a9006b7265cdcf7878a06cc828e916841625d58563a66b63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10529-015-2025-8$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10529-015-2025-8$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>315,781,785,27928,27929,41492,42561,51323</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26712371$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Joseph Sahaya Rajan, J</creatorcontrib><creatorcontrib>Chinnappan Santiago, T</creatorcontrib><creatorcontrib>Singaravel, R</creatorcontrib><creatorcontrib>Ignacimuthu, S</creatorcontrib><title>Outer membrane protein C (OmpC) of Escherichia coli induces neurodegeneration in mice by acting as an amyloid</title><title>Biotechnology letters</title><addtitle>Biotechnol Lett</addtitle><addtitle>Biotechnol Lett</addtitle><description>OBJECTIVES: Involvement of the outer membrane protein C (OmpC) of Escherichia coli in neurodegeneration was investigated using a mouse model. RESULTS: OmpC formed protease-resistant fibres that exhibited the diagnostic features of an amyloid. The spectral shift in the Congo Red and the thioflavin T assays produced features similar to neurotoxic peptides. Intramuscular administration of OmpC in mice resulted in spongiform neurodegeneration of the brain through calcium-dependent apoptosis and also showed upregulation of apoptosis related genes. Immunolocalization of OmpC in brain demonstrated the direct involvement of the porin in neurodegeneration and formation of spongiform encephalopathy. CONCLUSION: We have demonstrated the ability of OmpC of E. coli to induce neurodegeneration in mice.</description><subject>amyloid</subject><subject>animal models</subject><subject>Animals</subject><subject>Apoptosis</subject><subject>Applied Microbiology</subject><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Biotechnology</subject><subject>Brain</subject><subject>Brain - metabolism</subject><subject>Brain - pathology</subject><subject>Calcium - metabolism</subject><subject>Disease Models, Animal</subject><subject>E coli</subject><subject>encephalopathy</subject><subject>Escherichia coli</subject><subject>Fibers</subject><subject>Gene Expression Regulation</subject><subject>genes</subject><subject>Injections, Intramuscular</subject><subject>intramuscular injection</subject><subject>Life Sciences</subject><subject>Membranes</subject><subject>Mice</subject><subject>Microbiology</subject><subject>Neurodegeneration</subject><subject>neurodegenerative diseases</subject><subject>Neurodegenerative Diseases - etiology</subject><subject>Neurodegenerative Diseases - metabolism</subject><subject>Neurodegenerative Diseases - pathology</subject><subject>neurotoxicity</subject><subject>Original Research Paper</subject><subject>outer membrane proteins</subject><subject>Peptides</subject><subject>porins</subject><subject>Porins - administration & dosage</subject><subject>Porins - toxicity</subject><subject>Proteases</subject><subject>Proteins</subject><subject>Spectra</subject><subject>Spongiform 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Sahaya Rajan, J</creator><creator>Chinnappan Santiago, T</creator><creator>Singaravel, R</creator><creator>Ignacimuthu, S</creator><general>Springer Netherlands</general><general>Springer Nature 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Lett</addtitle><date>2016-04-01</date><risdate>2016</risdate><volume>38</volume><issue>4</issue><spage>689</spage><epage>700</epage><pages>689-700</pages><issn>0141-5492</issn><eissn>1573-6776</eissn><abstract>OBJECTIVES: Involvement of the outer membrane protein C (OmpC) of Escherichia coli in neurodegeneration was investigated using a mouse model. RESULTS: OmpC formed protease-resistant fibres that exhibited the diagnostic features of an amyloid. The spectral shift in the Congo Red and the thioflavin T assays produced features similar to neurotoxic peptides. Intramuscular administration of OmpC in mice resulted in spongiform neurodegeneration of the brain through calcium-dependent apoptosis and also showed upregulation of apoptosis related genes. Immunolocalization of OmpC in brain demonstrated the direct involvement of the porin in neurodegeneration and formation of spongiform encephalopathy. CONCLUSION: We have demonstrated the ability of OmpC of E. coli to induce neurodegeneration in mice.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>26712371</pmid><doi>10.1007/s10529-015-2025-8</doi><tpages>12</tpages></addata></record> |
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| ispartof | Biotechnology letters, 2016-04, Vol.38 (4), p.689-700 |
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| subjects | amyloid animal models Animals Apoptosis Applied Microbiology Biochemistry Biomedical and Life Sciences Biotechnology Brain Brain - metabolism Brain - pathology Calcium - metabolism Disease Models, Animal E coli encephalopathy Escherichia coli Fibers Gene Expression Regulation genes Injections, Intramuscular intramuscular injection Life Sciences Membranes Mice Microbiology Neurodegeneration neurodegenerative diseases Neurodegenerative Diseases - etiology Neurodegenerative Diseases - metabolism Neurodegenerative Diseases - pathology neurotoxicity Original Research Paper outer membrane proteins Peptides porins Porins - administration & dosage Porins - toxicity Proteases Proteins Spectra Spongiform encephalopathies |
| title | Outer membrane protein C (OmpC) of Escherichia coli induces neurodegeneration in mice by acting as an amyloid |
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