Development of a rapid column-switching LC-MS/MS method for the quantification of THCCOOH and THCCOOH-glucuronide in whole blood for assessing cannabis consumption frequency
The concentration of 11-nor-9-carboxy-Δ⁹-tetrahydrocannabinol (THCCOOH) in whole blood is used as a parameter for assessing the consumption behavior of cannabis consumers. The blood level of THCCOOH-glucuronide might provide additional information about the frequency of cannabis use. To verify this...
Gespeichert in:
| Veröffentlicht in: | Analytical and bioanalytical chemistry 2016-03, Vol.408 (7), p.1953-1962 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1962 |
|---|---|
| container_issue | 7 |
| container_start_page | 1953 |
| container_title | Analytical and bioanalytical chemistry |
| container_volume | 408 |
| creator | Hädener, Marianne Weinmann, Wolfgang Schürch, Stefan König, Stefan |
| description | The concentration of 11-nor-9-carboxy-Δ⁹-tetrahydrocannabinol (THCCOOH) in whole blood is used as a parameter for assessing the consumption behavior of cannabis consumers. The blood level of THCCOOH-glucuronide might provide additional information about the frequency of cannabis use. To verify this assumption, a column-switching liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the rapid and direct quantification of free and glucuronidated THCCOOH in human whole blood was newly developed. The method comprised protein precipitation, followed by injection of the processed sample onto a trapping column and subsequent gradient elution to an analytical column for separation and detection. The total LC run time was 4.5 min. Detection of the analytes was accomplished by electrospray ionization in positive ion mode and selected reaction monitoring using a triple-stage quadrupole mass spectrometer. The method was fully validated by evaluating the following parameters: linearity, lower limit of quantification, accuracy and imprecision, selectivity, extraction efficiency, matrix effect, carry-over, dilution integrity, analyte stability, and re-injection reproducibility. All acceptance criteria were analyzed and the predefined criteria met. Linearity ranged from 5.0 to 500 μg/L for both analytes. The method was successfully applied to whole blood samples from a large collective of cannabis consumers, demonstrating its applicability in the forensic field. |
| doi_str_mv | 10.1007/s00216-016-9311-8 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_proqu</sourceid><recordid>TN_cdi_proquest_miscellaneous_1800503686</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A443688731</galeid><sourcerecordid>A443688731</sourcerecordid><originalsourceid>FETCH-LOGICAL-c566t-9d3aa7f13a2a57df6344d4d149bd7ba9c6a093860a0bc2574dd71036175412603</originalsourceid><addsrcrecordid>eNqNks1u3CAUha2qVZOmfYBuWqRuunECGANeRu7PVJpoFpOsEeZnhsiGCdiJ8lB9x-LOJEqrqq0QAsF3DveiUxRvETxFELKzBCFGtIR5NhVCJX9WHCOKeIlpDZ8_7gk-Kl6ldA0hqjmiL4sjTBlHCLLj4vsnc2v6sBuMH0GwQIIod04DFfpp8GW6c6PaOr8By7a8WJ9drMFgxm3QwIYIxq0BN5P0o7NOydEFP1tcLtp2tVoA6fXDvtz0k5pi8E4b4Dy424begK4PByOZkklpfkZJ72XnUi7Ap2nY_TS10dxMxqv718ULK_tk3hzWk-Lqy-fLdlEuV1-_tefLUtWUjmWjKymZRZXEsmba0ooQTTQiTadZJxtFJWwqTqGEncI1I1ozBCuKWE0QprA6KT7ufXcx5JfTKAaXlOl76U2YkkAcwjoLOP03yjisEcM1_w-UMsiyaZPRD7-h12GKPvecKQY5gTV5Qm1kb4TzNoxRqtlUnBOSy-OsQpk6_QOVhzaDy99srMvnvwjQXqBiSCkaK3bRDTLeCwTFHDyxD57IwRNz8MTc27tDwVM3GP2oeEhaBvAeSPnKb0x80tFfXN_vRVYGITfRJXG1xhnIUcaIMFL9AMGq6eU</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1770840549</pqid></control><display><type>article</type><title>Development of a rapid column-switching LC-MS/MS method for the quantification of THCCOOH and THCCOOH-glucuronide in whole blood for assessing cannabis consumption frequency</title><source>MEDLINE</source><source>Springer Nature - Complete Springer Journals</source><creator>Hädener, Marianne ; Weinmann, Wolfgang ; Schürch, Stefan ; König, Stefan</creator><creatorcontrib>Hädener, Marianne ; Weinmann, Wolfgang ; Schürch, Stefan ; König, Stefan</creatorcontrib><description>The concentration of 11-nor-9-carboxy-Δ⁹-tetrahydrocannabinol (THCCOOH) in whole blood is used as a parameter for assessing the consumption behavior of cannabis consumers. The blood level of THCCOOH-glucuronide might provide additional information about the frequency of cannabis use. To verify this assumption, a column-switching liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the rapid and direct quantification of free and glucuronidated THCCOOH in human whole blood was newly developed. The method comprised protein precipitation, followed by injection of the processed sample onto a trapping column and subsequent gradient elution to an analytical column for separation and detection. The total LC run time was 4.5 min. Detection of the analytes was accomplished by electrospray ionization in positive ion mode and selected reaction monitoring using a triple-stage quadrupole mass spectrometer. The method was fully validated by evaluating the following parameters: linearity, lower limit of quantification, accuracy and imprecision, selectivity, extraction efficiency, matrix effect, carry-over, dilution integrity, analyte stability, and re-injection reproducibility. All acceptance criteria were analyzed and the predefined criteria met. Linearity ranged from 5.0 to 500 μg/L for both analytes. The method was successfully applied to whole blood samples from a large collective of cannabis consumers, demonstrating its applicability in the forensic field.</description><identifier>ISSN: 1618-2642</identifier><identifier>EISSN: 1618-2650</identifier><identifier>DOI: 10.1007/s00216-016-9311-8</identifier><identifier>PMID: 26781107</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Analysis ; Analytical Chemistry ; Biochemistry ; Biomimetics ; Blood ; Blood tests ; Cannabis ; Cannabis - chemistry ; Characterization and Evaluation of Materials ; Chemical properties ; Chemistry ; Chemistry and Materials Science ; Chromatography ; Chromatography, High Pressure Liquid - economics ; Chromatography, High Pressure Liquid - instrumentation ; Chromatography, High Pressure Liquid - methods ; Consumers ; Consumption ; Dronabinol - analogs & derivatives ; Dronabinol - blood ; Drunk driving ; Enzymes ; Equipment Design ; Food Science ; forensic sciences ; Glucuronides - blood ; Humans ; Injection ; Ionization ; Laboratory Medicine ; Limit of Detection ; Linearity ; Liquid chromatography ; Marijuana ; Marijuana Smoking - blood ; Mass spectrometry ; Mathematical analysis ; Methods ; monitoring ; Monitoring/Environmental Analysis ; Reproducibility of Results ; Research Paper ; Scientific imaging ; spectrometers ; Substance Abuse Detection - economics ; Substance Abuse Detection - instrumentation ; Substance Abuse Detection - methods ; tandem mass spectrometry ; Tandem Mass Spectrometry - economics ; Tandem Mass Spectrometry - instrumentation ; Tandem Mass Spectrometry - methods ; Tetrahydrocannabinol ; THC ; Trapping</subject><ispartof>Analytical and bioanalytical chemistry, 2016-03, Vol.408 (7), p.1953-1962</ispartof><rights>Springer-Verlag Berlin Heidelberg 2016</rights><rights>COPYRIGHT 2016 Springer</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c566t-9d3aa7f13a2a57df6344d4d149bd7ba9c6a093860a0bc2574dd71036175412603</citedby><cites>FETCH-LOGICAL-c566t-9d3aa7f13a2a57df6344d4d149bd7ba9c6a093860a0bc2574dd71036175412603</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00216-016-9311-8$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00216-016-9311-8$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26781107$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hädener, Marianne</creatorcontrib><creatorcontrib>Weinmann, Wolfgang</creatorcontrib><creatorcontrib>Schürch, Stefan</creatorcontrib><creatorcontrib>König, Stefan</creatorcontrib><title>Development of a rapid column-switching LC-MS/MS method for the quantification of THCCOOH and THCCOOH-glucuronide in whole blood for assessing cannabis consumption frequency</title><title>Analytical and bioanalytical chemistry</title><addtitle>Anal Bioanal Chem</addtitle><addtitle>Anal Bioanal Chem</addtitle><description>The concentration of 11-nor-9-carboxy-Δ⁹-tetrahydrocannabinol (THCCOOH) in whole blood is used as a parameter for assessing the consumption behavior of cannabis consumers. The blood level of THCCOOH-glucuronide might provide additional information about the frequency of cannabis use. To verify this assumption, a column-switching liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the rapid and direct quantification of free and glucuronidated THCCOOH in human whole blood was newly developed. The method comprised protein precipitation, followed by injection of the processed sample onto a trapping column and subsequent gradient elution to an analytical column for separation and detection. The total LC run time was 4.5 min. Detection of the analytes was accomplished by electrospray ionization in positive ion mode and selected reaction monitoring using a triple-stage quadrupole mass spectrometer. The method was fully validated by evaluating the following parameters: linearity, lower limit of quantification, accuracy and imprecision, selectivity, extraction efficiency, matrix effect, carry-over, dilution integrity, analyte stability, and re-injection reproducibility. All acceptance criteria were analyzed and the predefined criteria met. Linearity ranged from 5.0 to 500 μg/L for both analytes. The method was successfully applied to whole blood samples from a large collective of cannabis consumers, demonstrating its applicability in the forensic field.</description><subject>Analysis</subject><subject>Analytical Chemistry</subject><subject>Biochemistry</subject><subject>Biomimetics</subject><subject>Blood</subject><subject>Blood tests</subject><subject>Cannabis</subject><subject>Cannabis - chemistry</subject><subject>Characterization and Evaluation of Materials</subject><subject>Chemical properties</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Chromatography</subject><subject>Chromatography, High Pressure Liquid - economics</subject><subject>Chromatography, High Pressure Liquid - instrumentation</subject><subject>Chromatography, High Pressure Liquid - methods</subject><subject>Consumers</subject><subject>Consumption</subject><subject>Dronabinol - analogs & derivatives</subject><subject>Dronabinol - blood</subject><subject>Drunk driving</subject><subject>Enzymes</subject><subject>Equipment Design</subject><subject>Food Science</subject><subject>forensic sciences</subject><subject>Glucuronides - blood</subject><subject>Humans</subject><subject>Injection</subject><subject>Ionization</subject><subject>Laboratory Medicine</subject><subject>Limit of Detection</subject><subject>Linearity</subject><subject>Liquid chromatography</subject><subject>Marijuana</subject><subject>Marijuana Smoking - blood</subject><subject>Mass spectrometry</subject><subject>Mathematical analysis</subject><subject>Methods</subject><subject>monitoring</subject><subject>Monitoring/Environmental Analysis</subject><subject>Reproducibility of Results</subject><subject>Research Paper</subject><subject>Scientific imaging</subject><subject>spectrometers</subject><subject>Substance Abuse Detection - economics</subject><subject>Substance Abuse Detection - instrumentation</subject><subject>Substance Abuse Detection - methods</subject><subject>tandem mass spectrometry</subject><subject>Tandem Mass Spectrometry - economics</subject><subject>Tandem Mass Spectrometry - instrumentation</subject><subject>Tandem Mass Spectrometry - methods</subject><subject>Tetrahydrocannabinol</subject><subject>THC</subject><subject>Trapping</subject><issn>1618-2642</issn><issn>1618-2650</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqNks1u3CAUha2qVZOmfYBuWqRuunECGANeRu7PVJpoFpOsEeZnhsiGCdiJ8lB9x-LOJEqrqq0QAsF3DveiUxRvETxFELKzBCFGtIR5NhVCJX9WHCOKeIlpDZ8_7gk-Kl6ldA0hqjmiL4sjTBlHCLLj4vsnc2v6sBuMH0GwQIIod04DFfpp8GW6c6PaOr8By7a8WJ9drMFgxm3QwIYIxq0BN5P0o7NOydEFP1tcLtp2tVoA6fXDvtz0k5pi8E4b4Dy424begK4PByOZkklpfkZJ72XnUi7Ap2nY_TS10dxMxqv718ULK_tk3hzWk-Lqy-fLdlEuV1-_tefLUtWUjmWjKymZRZXEsmba0ooQTTQiTadZJxtFJWwqTqGEncI1I1ozBCuKWE0QprA6KT7ufXcx5JfTKAaXlOl76U2YkkAcwjoLOP03yjisEcM1_w-UMsiyaZPRD7-h12GKPvecKQY5gTV5Qm1kb4TzNoxRqtlUnBOSy-OsQpk6_QOVhzaDy99srMvnvwjQXqBiSCkaK3bRDTLeCwTFHDyxD57IwRNz8MTc27tDwVM3GP2oeEhaBvAeSPnKb0x80tFfXN_vRVYGITfRJXG1xhnIUcaIMFL9AMGq6eU</recordid><startdate>20160301</startdate><enddate>20160301</enddate><creator>Hädener, Marianne</creator><creator>Weinmann, Wolfgang</creator><creator>Schürch, Stefan</creator><creator>König, Stefan</creator><general>Springer Berlin Heidelberg</general><general>Springer</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>7U5</scope><scope>7U7</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8BQ</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>F28</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H8D</scope><scope>H8G</scope><scope>HCIFZ</scope><scope>JG9</scope><scope>JQ2</scope><scope>K9.</scope><scope>KB.</scope><scope>KR7</scope><scope>L7M</scope><scope>LK8</scope><scope>L~C</scope><scope>L~D</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>P64</scope><scope>PDBOC</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7X8</scope></search><sort><creationdate>20160301</creationdate><title>Development of a rapid column-switching LC-MS/MS method for the quantification of THCCOOH and THCCOOH-glucuronide in whole blood for assessing cannabis consumption frequency</title><author>Hädener, Marianne ; Weinmann, Wolfgang ; Schürch, Stefan ; König, Stefan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c566t-9d3aa7f13a2a57df6344d4d149bd7ba9c6a093860a0bc2574dd71036175412603</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Analysis</topic><topic>Analytical Chemistry</topic><topic>Biochemistry</topic><topic>Biomimetics</topic><topic>Blood</topic><topic>Blood tests</topic><topic>Cannabis</topic><topic>Cannabis - chemistry</topic><topic>Characterization and Evaluation of Materials</topic><topic>Chemical properties</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Chromatography</topic><topic>Chromatography, High Pressure Liquid - economics</topic><topic>Chromatography, High Pressure Liquid - instrumentation</topic><topic>Chromatography, High Pressure Liquid - methods</topic><topic>Consumers</topic><topic>Consumption</topic><topic>Dronabinol - analogs & derivatives</topic><topic>Dronabinol - blood</topic><topic>Drunk driving</topic><topic>Enzymes</topic><topic>Equipment Design</topic><topic>Food Science</topic><topic>forensic sciences</topic><topic>Glucuronides - blood</topic><topic>Humans</topic><topic>Injection</topic><topic>Ionization</topic><topic>Laboratory Medicine</topic><topic>Limit of Detection</topic><topic>Linearity</topic><topic>Liquid chromatography</topic><topic>Marijuana</topic><topic>Marijuana Smoking - blood</topic><topic>Mass spectrometry</topic><topic>Mathematical analysis</topic><topic>Methods</topic><topic>monitoring</topic><topic>Monitoring/Environmental Analysis</topic><topic>Reproducibility of Results</topic><topic>Research Paper</topic><topic>Scientific imaging</topic><topic>spectrometers</topic><topic>Substance Abuse Detection - economics</topic><topic>Substance Abuse Detection - instrumentation</topic><topic>Substance Abuse Detection - methods</topic><topic>tandem mass spectrometry</topic><topic>Tandem Mass Spectrometry - economics</topic><topic>Tandem Mass Spectrometry - instrumentation</topic><topic>Tandem Mass Spectrometry - methods</topic><topic>Tetrahydrocannabinol</topic><topic>THC</topic><topic>Trapping</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hädener, Marianne</creatorcontrib><creatorcontrib>Weinmann, Wolfgang</creatorcontrib><creatorcontrib>Schürch, Stefan</creatorcontrib><creatorcontrib>König, Stefan</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>SciTech Premium Collection</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>ProQuest Biological Science Collection</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Materials Science Collection</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical and bioanalytical chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hädener, Marianne</au><au>Weinmann, Wolfgang</au><au>Schürch, Stefan</au><au>König, Stefan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development of a rapid column-switching LC-MS/MS method for the quantification of THCCOOH and THCCOOH-glucuronide in whole blood for assessing cannabis consumption frequency</atitle><jtitle>Analytical and bioanalytical chemistry</jtitle><stitle>Anal Bioanal Chem</stitle><addtitle>Anal Bioanal Chem</addtitle><date>2016-03-01</date><risdate>2016</risdate><volume>408</volume><issue>7</issue><spage>1953</spage><epage>1962</epage><pages>1953-1962</pages><issn>1618-2642</issn><eissn>1618-2650</eissn><abstract>The concentration of 11-nor-9-carboxy-Δ⁹-tetrahydrocannabinol (THCCOOH) in whole blood is used as a parameter for assessing the consumption behavior of cannabis consumers. The blood level of THCCOOH-glucuronide might provide additional information about the frequency of cannabis use. To verify this assumption, a column-switching liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the rapid and direct quantification of free and glucuronidated THCCOOH in human whole blood was newly developed. The method comprised protein precipitation, followed by injection of the processed sample onto a trapping column and subsequent gradient elution to an analytical column for separation and detection. The total LC run time was 4.5 min. Detection of the analytes was accomplished by electrospray ionization in positive ion mode and selected reaction monitoring using a triple-stage quadrupole mass spectrometer. The method was fully validated by evaluating the following parameters: linearity, lower limit of quantification, accuracy and imprecision, selectivity, extraction efficiency, matrix effect, carry-over, dilution integrity, analyte stability, and re-injection reproducibility. All acceptance criteria were analyzed and the predefined criteria met. Linearity ranged from 5.0 to 500 μg/L for both analytes. The method was successfully applied to whole blood samples from a large collective of cannabis consumers, demonstrating its applicability in the forensic field.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>26781107</pmid><doi>10.1007/s00216-016-9311-8</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1618-2642 |
| ispartof | Analytical and bioanalytical chemistry, 2016-03, Vol.408 (7), p.1953-1962 |
| issn | 1618-2642 1618-2650 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1800503686 |
| source | MEDLINE; Springer Nature - Complete Springer Journals |
| subjects | Analysis Analytical Chemistry Biochemistry Biomimetics Blood Blood tests Cannabis Cannabis - chemistry Characterization and Evaluation of Materials Chemical properties Chemistry Chemistry and Materials Science Chromatography Chromatography, High Pressure Liquid - economics Chromatography, High Pressure Liquid - instrumentation Chromatography, High Pressure Liquid - methods Consumers Consumption Dronabinol - analogs & derivatives Dronabinol - blood Drunk driving Enzymes Equipment Design Food Science forensic sciences Glucuronides - blood Humans Injection Ionization Laboratory Medicine Limit of Detection Linearity Liquid chromatography Marijuana Marijuana Smoking - blood Mass spectrometry Mathematical analysis Methods monitoring Monitoring/Environmental Analysis Reproducibility of Results Research Paper Scientific imaging spectrometers Substance Abuse Detection - economics Substance Abuse Detection - instrumentation Substance Abuse Detection - methods tandem mass spectrometry Tandem Mass Spectrometry - economics Tandem Mass Spectrometry - instrumentation Tandem Mass Spectrometry - methods Tetrahydrocannabinol THC Trapping |
| title | Development of a rapid column-switching LC-MS/MS method for the quantification of THCCOOH and THCCOOH-glucuronide in whole blood for assessing cannabis consumption frequency |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-31T21%3A03%3A53IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_proqu&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Development%20of%20a%20rapid%20column-switching%20LC-MS/MS%20method%20for%20the%20quantification%20of%20THCCOOH%20and%20THCCOOH-glucuronide%20in%20whole%20blood%20for%20assessing%20cannabis%20consumption%20frequency&rft.jtitle=Analytical%20and%20bioanalytical%20chemistry&rft.au=H%C3%A4dener,%20Marianne&rft.date=2016-03-01&rft.volume=408&rft.issue=7&rft.spage=1953&rft.epage=1962&rft.pages=1953-1962&rft.issn=1618-2642&rft.eissn=1618-2650&rft_id=info:doi/10.1007/s00216-016-9311-8&rft_dat=%3Cgale_proqu%3EA443688731%3C/gale_proqu%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1770840549&rft_id=info:pmid/26781107&rft_galeid=A443688731&rfr_iscdi=true |