Functional characterization of recombinant human granulocyte colony stimulating factor (hGMCSF) immobilized onto silica nanoparticles
OBJECTIVES: Granulocyte macrophage colony stimulating factor (GMCSF), an important therapeutic cytokine, was immobilized onto silica nanoparticles. Maintenance of structural integrity and biological performance in immobilized cytokine was assessed to augment its applicability in possible biomedical...
Gespeichert in:
| Veröffentlicht in: | Biotechnology letters 2016-02, Vol.38 (2), p.243-249 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 249 |
|---|---|
| container_issue | 2 |
| container_start_page | 243 |
| container_title | Biotechnology letters |
| container_volume | 38 |
| creator | Vanitha, Selvarajan Goswami, Upashi Chaubey, Nidhi Ghosh, Siddhartha S Sanpui, Pallab |
| description | OBJECTIVES: Granulocyte macrophage colony stimulating factor (GMCSF), an important therapeutic cytokine, was immobilized onto silica nanoparticles. Maintenance of structural integrity and biological performance in immobilized cytokine was assessed to augment its applicability in possible biomedical implications. RESULTS: Following its cloning and expression in E. coli, the recombinant human GMCSF (hGMCSF) was purified as a GST-tagged protein corresponding to a 42 kDa band on SDS-PAGE. The purified cytokine was immobilized onto biocompatible silica nanoparticles (~129.4 nm) by adsorption and the binding was confirmed by dynamic light scattering and infrared spectroscopy. Maximum binding of hGMCSF was at 6.4 µg mg⁻¹ silica nanoparticles. Efficient release of the cytokine from the nanoparticles with its structural integrity intact was deduced from circular dichroism spectroscopy. hGMCSF-immobilized silica nanoparticles efficiently increased the proliferation of RAW 264.7 macrophage cells with 50 % increase in proliferation at 600 ng hGMCSF µg⁻¹ silica nanoparticles. CONCLUSIONS: Silica nanoparticles successfully immobilized hGMCSF maintaining its structural integrity. The release of the immobilized cytokine from silica nanoparticles resulted in the increased proliferation of macrophages indicating the potential of the system in future applications. |
| doi_str_mv | 10.1007/s10529-015-1984-0 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1800500201</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>3973628591</sourcerecordid><originalsourceid>FETCH-LOGICAL-c532t-fe8aa19244ac707df5d3ee38581c6892424711f33ff066fef1162917c93551ed3</originalsourceid><addsrcrecordid>eNqNkUFv1DAQhS0EokvhB3ABS1zKITBjx05yRCu2IBVxKD1bXsfedZXYi50ctnf-N15SEOIAnCyPv_dmPI-Q5whvEKB5mxEE6ypAUWHX1hU8ICsUDa9k08iHZAVYYyXqjp2RJznfAkDXQPOYnDFZqrxpV-TbZg5m8jHogZq9TtpMNvk7fSrR6GiyJo5bH3SY6H4edaC7pMM8RHOcLDVxiOFI8-THeSiasKOuOMREL_aXn9bXm9fUj2Pc-sHf2Z7GMEWay8VoWhzjQafJm8Hmp-SR00O2z-7Pc3Kzef9l_aG6-nz5cf3uqjKCs6lyttUaO1bX2pSP9E703FreihaNbEud1Q2i49w5kNJZhyhZh43puBBoe35OLhbfQ4pfZ5snNfps7DDoYOOcFbYAAoAB_hstK5YCOK__A5WSSd5IUdBXf6C3cU5l-T8MEaFDPPXGhTIp5pysU4fkR52OCkGdkldL8qokr07JKyiaF_fO83a0_S_Fz6gLwBYgl6ews-m31n9xfbmInI5K75LP6ua6rEcClGEFAv8OikPCcg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1771109111</pqid></control><display><type>article</type><title>Functional characterization of recombinant human granulocyte colony stimulating factor (hGMCSF) immobilized onto silica nanoparticles</title><source>MEDLINE</source><source>SpringerLink Journals - AutoHoldings</source><creator>Vanitha, Selvarajan ; Goswami, Upashi ; Chaubey, Nidhi ; Ghosh, Siddhartha S ; Sanpui, Pallab</creator><creatorcontrib>Vanitha, Selvarajan ; Goswami, Upashi ; Chaubey, Nidhi ; Ghosh, Siddhartha S ; Sanpui, Pallab</creatorcontrib><description>OBJECTIVES: Granulocyte macrophage colony stimulating factor (GMCSF), an important therapeutic cytokine, was immobilized onto silica nanoparticles. Maintenance of structural integrity and biological performance in immobilized cytokine was assessed to augment its applicability in possible biomedical implications. RESULTS: Following its cloning and expression in E. coli, the recombinant human GMCSF (hGMCSF) was purified as a GST-tagged protein corresponding to a 42 kDa band on SDS-PAGE. The purified cytokine was immobilized onto biocompatible silica nanoparticles (~129.4 nm) by adsorption and the binding was confirmed by dynamic light scattering and infrared spectroscopy. Maximum binding of hGMCSF was at 6.4 µg mg⁻¹ silica nanoparticles. Efficient release of the cytokine from the nanoparticles with its structural integrity intact was deduced from circular dichroism spectroscopy. hGMCSF-immobilized silica nanoparticles efficiently increased the proliferation of RAW 264.7 macrophage cells with 50 % increase in proliferation at 600 ng hGMCSF µg⁻¹ silica nanoparticles. CONCLUSIONS: Silica nanoparticles successfully immobilized hGMCSF maintaining its structural integrity. The release of the immobilized cytokine from silica nanoparticles resulted in the increased proliferation of macrophages indicating the potential of the system in future applications.</description><identifier>ISSN: 0141-5492</identifier><identifier>EISSN: 1573-6776</identifier><identifier>DOI: 10.1007/s10529-015-1984-0</identifier><identifier>PMID: 26549378</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Adsorption ; Animals ; Applied Microbiology ; Binding ; Biochemistry ; Biomedical and Life Sciences ; Biomedical materials ; Biotechnology ; Cell Proliferation - drug effects ; Circular Dichroism ; circular dichroism spectroscopy ; Cloning ; Cloning, Molecular ; colony-stimulating factors ; Cytokines ; Drug Carriers ; Dynamic Light Scattering ; E coli ; Escherichia coli ; Escherichia coli - genetics ; Escherichia coli - metabolism ; Gene Expression ; Granulocyte Colony-Stimulating Factor - chemistry ; Granulocyte Colony-Stimulating Factor - isolation & purification ; Granulocyte Colony-Stimulating Factor - metabolism ; Human ; Humans ; Immobilized Proteins - chemistry ; Immobilized Proteins - isolation & purification ; Immobilized Proteins - metabolism ; Infrared spectroscopy ; Life Sciences ; Light scattering ; Macrophages ; Macrophages - drug effects ; Macrophages - physiology ; Mice ; Microbiology ; Molecular Weight ; Nanoparticles ; Original Research Paper ; polyacrylamide gel electrophoresis ; Proteins ; RAW 264.7 Cells ; Recombinant ; Recombinant Proteins - chemistry ; Recombinant Proteins - isolation & purification ; Recombinant Proteins - metabolism ; Silica ; Silicon Dioxide ; Spectrophotometry, Infrared ; Structural integrity</subject><ispartof>Biotechnology letters, 2016-02, Vol.38 (2), p.243-249</ispartof><rights>Springer Science+Business Media Dordrecht 2015</rights><rights>Springer Science+Business Media Dordrecht 2016</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c532t-fe8aa19244ac707df5d3ee38581c6892424711f33ff066fef1162917c93551ed3</citedby><cites>FETCH-LOGICAL-c532t-fe8aa19244ac707df5d3ee38581c6892424711f33ff066fef1162917c93551ed3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10529-015-1984-0$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10529-015-1984-0$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26549378$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Vanitha, Selvarajan</creatorcontrib><creatorcontrib>Goswami, Upashi</creatorcontrib><creatorcontrib>Chaubey, Nidhi</creatorcontrib><creatorcontrib>Ghosh, Siddhartha S</creatorcontrib><creatorcontrib>Sanpui, Pallab</creatorcontrib><title>Functional characterization of recombinant human granulocyte colony stimulating factor (hGMCSF) immobilized onto silica nanoparticles</title><title>Biotechnology letters</title><addtitle>Biotechnol Lett</addtitle><addtitle>Biotechnol Lett</addtitle><description>OBJECTIVES: Granulocyte macrophage colony stimulating factor (GMCSF), an important therapeutic cytokine, was immobilized onto silica nanoparticles. Maintenance of structural integrity and biological performance in immobilized cytokine was assessed to augment its applicability in possible biomedical implications. RESULTS: Following its cloning and expression in E. coli, the recombinant human GMCSF (hGMCSF) was purified as a GST-tagged protein corresponding to a 42 kDa band on SDS-PAGE. The purified cytokine was immobilized onto biocompatible silica nanoparticles (~129.4 nm) by adsorption and the binding was confirmed by dynamic light scattering and infrared spectroscopy. Maximum binding of hGMCSF was at 6.4 µg mg⁻¹ silica nanoparticles. Efficient release of the cytokine from the nanoparticles with its structural integrity intact was deduced from circular dichroism spectroscopy. hGMCSF-immobilized silica nanoparticles efficiently increased the proliferation of RAW 264.7 macrophage cells with 50 % increase in proliferation at 600 ng hGMCSF µg⁻¹ silica nanoparticles. CONCLUSIONS: Silica nanoparticles successfully immobilized hGMCSF maintaining its structural integrity. The release of the immobilized cytokine from silica nanoparticles resulted in the increased proliferation of macrophages indicating the potential of the system in future applications.</description><subject>Adsorption</subject><subject>Animals</subject><subject>Applied Microbiology</subject><subject>Binding</subject><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedical materials</subject><subject>Biotechnology</subject><subject>Cell Proliferation - drug effects</subject><subject>Circular Dichroism</subject><subject>circular dichroism spectroscopy</subject><subject>Cloning</subject><subject>Cloning, Molecular</subject><subject>colony-stimulating factors</subject><subject>Cytokines</subject><subject>Drug Carriers</subject><subject>Dynamic Light Scattering</subject><subject>E coli</subject><subject>Escherichia coli</subject><subject>Escherichia coli - genetics</subject><subject>Escherichia coli - metabolism</subject><subject>Gene Expression</subject><subject>Granulocyte Colony-Stimulating Factor - chemistry</subject><subject>Granulocyte Colony-Stimulating Factor - isolation & purification</subject><subject>Granulocyte Colony-Stimulating Factor - metabolism</subject><subject>Human</subject><subject>Humans</subject><subject>Immobilized Proteins - chemistry</subject><subject>Immobilized Proteins - isolation & purification</subject><subject>Immobilized Proteins - metabolism</subject><subject>Infrared spectroscopy</subject><subject>Life Sciences</subject><subject>Light scattering</subject><subject>Macrophages</subject><subject>Macrophages - drug effects</subject><subject>Macrophages - physiology</subject><subject>Mice</subject><subject>Microbiology</subject><subject>Molecular Weight</subject><subject>Nanoparticles</subject><subject>Original Research Paper</subject><subject>polyacrylamide gel electrophoresis</subject><subject>Proteins</subject><subject>RAW 264.7 Cells</subject><subject>Recombinant</subject><subject>Recombinant Proteins - chemistry</subject><subject>Recombinant Proteins - isolation & purification</subject><subject>Recombinant Proteins - metabolism</subject><subject>Silica</subject><subject>Silicon Dioxide</subject><subject>Spectrophotometry, Infrared</subject><subject>Structural integrity</subject><issn>0141-5492</issn><issn>1573-6776</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqNkUFv1DAQhS0EokvhB3ABS1zKITBjx05yRCu2IBVxKD1bXsfedZXYi50ctnf-N15SEOIAnCyPv_dmPI-Q5whvEKB5mxEE6ypAUWHX1hU8ICsUDa9k08iHZAVYYyXqjp2RJznfAkDXQPOYnDFZqrxpV-TbZg5m8jHogZq9TtpMNvk7fSrR6GiyJo5bH3SY6H4edaC7pMM8RHOcLDVxiOFI8-THeSiasKOuOMREL_aXn9bXm9fUj2Pc-sHf2Z7GMEWay8VoWhzjQafJm8Hmp-SR00O2z-7Pc3Kzef9l_aG6-nz5cf3uqjKCs6lyttUaO1bX2pSP9E703FreihaNbEud1Q2i49w5kNJZhyhZh43puBBoe35OLhbfQ4pfZ5snNfps7DDoYOOcFbYAAoAB_hstK5YCOK__A5WSSd5IUdBXf6C3cU5l-T8MEaFDPPXGhTIp5pysU4fkR52OCkGdkldL8qokr07JKyiaF_fO83a0_S_Fz6gLwBYgl6ews-m31n9xfbmInI5K75LP6ua6rEcClGEFAv8OikPCcg</recordid><startdate>20160201</startdate><enddate>20160201</enddate><creator>Vanitha, Selvarajan</creator><creator>Goswami, Upashi</creator><creator>Chaubey, Nidhi</creator><creator>Ghosh, Siddhartha S</creator><creator>Sanpui, Pallab</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7QR</scope><scope>7T7</scope><scope>7TB</scope><scope>7U5</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>L6V</scope><scope>L7M</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>Q9U</scope><scope>7X8</scope><scope>7QO</scope></search><sort><creationdate>20160201</creationdate><title>Functional characterization of recombinant human granulocyte colony stimulating factor (hGMCSF) immobilized onto silica nanoparticles</title><author>Vanitha, Selvarajan ; Goswami, Upashi ; Chaubey, Nidhi ; Ghosh, Siddhartha S ; Sanpui, Pallab</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c532t-fe8aa19244ac707df5d3ee38581c6892424711f33ff066fef1162917c93551ed3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Adsorption</topic><topic>Animals</topic><topic>Applied Microbiology</topic><topic>Binding</topic><topic>Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedical materials</topic><topic>Biotechnology</topic><topic>Cell Proliferation - drug effects</topic><topic>Circular Dichroism</topic><topic>circular dichroism spectroscopy</topic><topic>Cloning</topic><topic>Cloning, Molecular</topic><topic>colony-stimulating factors</topic><topic>Cytokines</topic><topic>Drug Carriers</topic><topic>Dynamic Light Scattering</topic><topic>E coli</topic><topic>Escherichia coli</topic><topic>Escherichia coli - genetics</topic><topic>Escherichia coli - metabolism</topic><topic>Gene Expression</topic><topic>Granulocyte Colony-Stimulating Factor - chemistry</topic><topic>Granulocyte Colony-Stimulating Factor - isolation & purification</topic><topic>Granulocyte Colony-Stimulating Factor - metabolism</topic><topic>Human</topic><topic>Humans</topic><topic>Immobilized Proteins - chemistry</topic><topic>Immobilized Proteins - isolation & purification</topic><topic>Immobilized Proteins - metabolism</topic><topic>Infrared spectroscopy</topic><topic>Life Sciences</topic><topic>Light scattering</topic><topic>Macrophages</topic><topic>Macrophages - drug effects</topic><topic>Macrophages - physiology</topic><topic>Mice</topic><topic>Microbiology</topic><topic>Molecular Weight</topic><topic>Nanoparticles</topic><topic>Original Research Paper</topic><topic>polyacrylamide gel electrophoresis</topic><topic>Proteins</topic><topic>RAW 264.7 Cells</topic><topic>Recombinant</topic><topic>Recombinant Proteins - chemistry</topic><topic>Recombinant Proteins - isolation & purification</topic><topic>Recombinant Proteins - metabolism</topic><topic>Silica</topic><topic>Silicon Dioxide</topic><topic>Spectrophotometry, Infrared</topic><topic>Structural integrity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Vanitha, Selvarajan</creatorcontrib><creatorcontrib>Goswami, Upashi</creatorcontrib><creatorcontrib>Chaubey, Nidhi</creatorcontrib><creatorcontrib>Ghosh, Siddhartha S</creatorcontrib><creatorcontrib>Sanpui, Pallab</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Chemoreception Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Engineering Collection</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Engineering Collection</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>Biotechnology Research Abstracts</collection><jtitle>Biotechnology letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Vanitha, Selvarajan</au><au>Goswami, Upashi</au><au>Chaubey, Nidhi</au><au>Ghosh, Siddhartha S</au><au>Sanpui, Pallab</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Functional characterization of recombinant human granulocyte colony stimulating factor (hGMCSF) immobilized onto silica nanoparticles</atitle><jtitle>Biotechnology letters</jtitle><stitle>Biotechnol Lett</stitle><addtitle>Biotechnol Lett</addtitle><date>2016-02-01</date><risdate>2016</risdate><volume>38</volume><issue>2</issue><spage>243</spage><epage>249</epage><pages>243-249</pages><issn>0141-5492</issn><eissn>1573-6776</eissn><abstract>OBJECTIVES: Granulocyte macrophage colony stimulating factor (GMCSF), an important therapeutic cytokine, was immobilized onto silica nanoparticles. Maintenance of structural integrity and biological performance in immobilized cytokine was assessed to augment its applicability in possible biomedical implications. RESULTS: Following its cloning and expression in E. coli, the recombinant human GMCSF (hGMCSF) was purified as a GST-tagged protein corresponding to a 42 kDa band on SDS-PAGE. The purified cytokine was immobilized onto biocompatible silica nanoparticles (~129.4 nm) by adsorption and the binding was confirmed by dynamic light scattering and infrared spectroscopy. Maximum binding of hGMCSF was at 6.4 µg mg⁻¹ silica nanoparticles. Efficient release of the cytokine from the nanoparticles with its structural integrity intact was deduced from circular dichroism spectroscopy. hGMCSF-immobilized silica nanoparticles efficiently increased the proliferation of RAW 264.7 macrophage cells with 50 % increase in proliferation at 600 ng hGMCSF µg⁻¹ silica nanoparticles. CONCLUSIONS: Silica nanoparticles successfully immobilized hGMCSF maintaining its structural integrity. The release of the immobilized cytokine from silica nanoparticles resulted in the increased proliferation of macrophages indicating the potential of the system in future applications.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>26549378</pmid><doi>10.1007/s10529-015-1984-0</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0141-5492 |
| ispartof | Biotechnology letters, 2016-02, Vol.38 (2), p.243-249 |
| issn | 0141-5492 1573-6776 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1800500201 |
| source | MEDLINE; SpringerLink Journals - AutoHoldings |
| subjects | Adsorption Animals Applied Microbiology Binding Biochemistry Biomedical and Life Sciences Biomedical materials Biotechnology Cell Proliferation - drug effects Circular Dichroism circular dichroism spectroscopy Cloning Cloning, Molecular colony-stimulating factors Cytokines Drug Carriers Dynamic Light Scattering E coli Escherichia coli Escherichia coli - genetics Escherichia coli - metabolism Gene Expression Granulocyte Colony-Stimulating Factor - chemistry Granulocyte Colony-Stimulating Factor - isolation & purification Granulocyte Colony-Stimulating Factor - metabolism Human Humans Immobilized Proteins - chemistry Immobilized Proteins - isolation & purification Immobilized Proteins - metabolism Infrared spectroscopy Life Sciences Light scattering Macrophages Macrophages - drug effects Macrophages - physiology Mice Microbiology Molecular Weight Nanoparticles Original Research Paper polyacrylamide gel electrophoresis Proteins RAW 264.7 Cells Recombinant Recombinant Proteins - chemistry Recombinant Proteins - isolation & purification Recombinant Proteins - metabolism Silica Silicon Dioxide Spectrophotometry, Infrared Structural integrity |
| title | Functional characterization of recombinant human granulocyte colony stimulating factor (hGMCSF) immobilized onto silica nanoparticles |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-04T02%3A07%3A52IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Functional%20characterization%20of%20recombinant%20human%20granulocyte%20colony%20stimulating%20factor%20(hGMCSF)%20immobilized%20onto%20silica%20nanoparticles&rft.jtitle=Biotechnology%20letters&rft.au=Vanitha,%20Selvarajan&rft.date=2016-02-01&rft.volume=38&rft.issue=2&rft.spage=243&rft.epage=249&rft.pages=243-249&rft.issn=0141-5492&rft.eissn=1573-6776&rft_id=info:doi/10.1007/s10529-015-1984-0&rft_dat=%3Cproquest_cross%3E3973628591%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1771109111&rft_id=info:pmid/26549378&rfr_iscdi=true |