Purification and molecular characterization of a novel mannose-specific lectin from Dioclea reflexa hook seeds with inflammatory activity
A novel lectin present in Dioclea reflexa seeds (DrfL) was discovered and described in this study. DrfL was purified in a single step by affinity chromatography in a Sephadex G‐50 column. The lectin strongly agglutinated rabbit erythrocytes and was inhibited by α‐methyl‐d‐mannoside, d‐mannose, and d...
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| Veröffentlicht in: | Journal of molecular recognition 2016-04, Vol.29 (4), p.134-141 |
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| creator | Pinto-Junior, Vanir R. Correia, Jorge L. A. Pereira, Ronniery I. Pereira-Junior, Francisco N. Santiago, Mayara Q. Osterne, Vinicius J. S. Madeira, Juliana C. Cajazeiras, João B. Nagano, Celso S. Delatorre, Plinio Assreuy, Ana M. S. Nascimento, Kyria S. Cavada, Benildo S. |
| description | A novel lectin present in Dioclea reflexa seeds (DrfL) was discovered and described in this study. DrfL was purified in a single step by affinity chromatography in a Sephadex G‐50 column. The lectin strongly agglutinated rabbit erythrocytes and was inhibited by α‐methyl‐d‐mannoside, d‐mannose, and d‐glucose. The hemagglutinating activity of DrfL is optimum at pH 5.0–7.0, stable up to 50 °C, and dependent on divalent cations. Similar to other lectins of the subtribe Diocleinae, the analysis by mass spectrometry indicated that DrfL has three chains (α, β, and γ) with masses of 25 562, 12 874, and 12 706 Da, respectively, with no disulfide bonds or glycosylation. DrfL showed inflammatory activity in the paw edema model and exhibited low cytotoxicity against Artemia sp. Copyright © 2015 John Wiley & Sons, Ltd.
A new mannose‐specific lectin from Dioclea reflexa Hook seeds (DrfL) was purified and partially characterized. DrfL toxic effect was evaluated against Artemia sp. DrfL elicits acute inflammation via lectin domain mediated by nitric oxide. |
| doi_str_mv | 10.1002/jmr.2512 |
| format | Article |
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A new mannose‐specific lectin from Dioclea reflexa Hook seeds (DrfL) was purified and partially characterized. DrfL toxic effect was evaluated against Artemia sp. DrfL elicits acute inflammation via lectin domain mediated by nitric oxide.</description><identifier>ISSN: 0952-3499</identifier><identifier>EISSN: 1099-1352</identifier><identifier>DOI: 10.1002/jmr.2512</identifier><identifier>PMID: 26464029</identifier><language>eng</language><publisher>England: Blackwell Publishing Ltd</publisher><subject>Animals ; Artemia ; Chromatography, Affinity ; Dioclea - chemistry ; Dioclea reflexa ; Edema ; Edema - chemically induced ; Erythrocytes ; Erythrocytes - drug effects ; ESI mass spectrometry ; Hemagglutination - drug effects ; Hooks ; Inflammation Mediators - isolation & purification ; Inflammation Mediators - pharmacology ; inflammatory activity ; lectin ; Lectins ; Mannose - pharmacology ; Mice ; Plant Lectins - chemistry ; Plant Lectins - isolation & purification ; Plant Lectins - pharmacology ; Protein Structure, Secondary ; Rabbits ; Recognition ; Seeds ; Toxic</subject><ispartof>Journal of molecular recognition, 2016-04, Vol.29 (4), p.134-141</ispartof><rights>Copyright © 2015 John Wiley & Sons, Ltd.</rights><rights>Copyright © 2016 John Wiley & Sons, Ltd.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4902-a5f7329b7ef41488e519faeb44c53ead099c7dafe402ab487c23944d732a6cdb3</citedby><cites>FETCH-LOGICAL-c4902-a5f7329b7ef41488e519faeb44c53ead099c7dafe402ab487c23944d732a6cdb3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjmr.2512$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjmr.2512$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>315,781,785,1418,27929,27930,45579,45580</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26464029$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pinto-Junior, Vanir R.</creatorcontrib><creatorcontrib>Correia, Jorge L. A.</creatorcontrib><creatorcontrib>Pereira, Ronniery I.</creatorcontrib><creatorcontrib>Pereira-Junior, Francisco N.</creatorcontrib><creatorcontrib>Santiago, Mayara Q.</creatorcontrib><creatorcontrib>Osterne, Vinicius J. S.</creatorcontrib><creatorcontrib>Madeira, Juliana C.</creatorcontrib><creatorcontrib>Cajazeiras, João B.</creatorcontrib><creatorcontrib>Nagano, Celso S.</creatorcontrib><creatorcontrib>Delatorre, Plinio</creatorcontrib><creatorcontrib>Assreuy, Ana M. S.</creatorcontrib><creatorcontrib>Nascimento, Kyria S.</creatorcontrib><creatorcontrib>Cavada, Benildo S.</creatorcontrib><title>Purification and molecular characterization of a novel mannose-specific lectin from Dioclea reflexa hook seeds with inflammatory activity</title><title>Journal of molecular recognition</title><addtitle>J. Mol. Recognit</addtitle><description>A novel lectin present in Dioclea reflexa seeds (DrfL) was discovered and described in this study. DrfL was purified in a single step by affinity chromatography in a Sephadex G‐50 column. The lectin strongly agglutinated rabbit erythrocytes and was inhibited by α‐methyl‐d‐mannoside, d‐mannose, and d‐glucose. The hemagglutinating activity of DrfL is optimum at pH 5.0–7.0, stable up to 50 °C, and dependent on divalent cations. Similar to other lectins of the subtribe Diocleinae, the analysis by mass spectrometry indicated that DrfL has three chains (α, β, and γ) with masses of 25 562, 12 874, and 12 706 Da, respectively, with no disulfide bonds or glycosylation. DrfL showed inflammatory activity in the paw edema model and exhibited low cytotoxicity against Artemia sp. Copyright © 2015 John Wiley & Sons, Ltd.
A new mannose‐specific lectin from Dioclea reflexa Hook seeds (DrfL) was purified and partially characterized. DrfL toxic effect was evaluated against Artemia sp. DrfL elicits acute inflammation via lectin domain mediated by nitric oxide.</description><subject>Animals</subject><subject>Artemia</subject><subject>Chromatography, Affinity</subject><subject>Dioclea - chemistry</subject><subject>Dioclea reflexa</subject><subject>Edema</subject><subject>Edema - chemically induced</subject><subject>Erythrocytes</subject><subject>Erythrocytes - drug effects</subject><subject>ESI mass spectrometry</subject><subject>Hemagglutination - drug effects</subject><subject>Hooks</subject><subject>Inflammation Mediators - isolation & purification</subject><subject>Inflammation Mediators - pharmacology</subject><subject>inflammatory activity</subject><subject>lectin</subject><subject>Lectins</subject><subject>Mannose - pharmacology</subject><subject>Mice</subject><subject>Plant Lectins - chemistry</subject><subject>Plant Lectins - isolation & purification</subject><subject>Plant Lectins - pharmacology</subject><subject>Protein Structure, Secondary</subject><subject>Rabbits</subject><subject>Recognition</subject><subject>Seeds</subject><subject>Toxic</subject><issn>0952-3499</issn><issn>1099-1352</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1u1DAUhS0EokNB4gmQJTZsUhzH-fESDTCASqlaEEvrxrnWeOrEUztpO7wBb42jGYqEhNj4Lu53jnXuIeR5zk5yxvjrTR9OeJnzB2SRMymzvCj5Q7JgsuRZIaQ8Ik9i3DCWdiV7TI54JSrBuFyQn-dTsMZqGK0fKAwd7b1DPTkIVK8hgB4x2B_7tTcU6OBv0NEehsFHzOIW9aynSTTagZrge_rWeu0QaEDj8A7o2vsrGhG7SG_tuKZ2MA76HkYfdjT9YG_suHtKHhlwEZ8d5jH59v7d1-WH7PTL6uPyzWmmhWQ8g9LUBZdtjUbkommwzKUBbIXQZYHQpYi67sBgygetaGrNCylEl0RQ6a4tjsmrve82-OsJ46h6GzU6BwP6Kaq8YUykw0n-f7ROrsX8JvTlX-jGT2FIQWYqdSObpvpjqIOPMZ1HbYPtIexUztTcpEpNqrnJhL44GE5tj909-Lu6BGR74NY63P3TSH36fHEwPPA2jnh3z0O4UlVd1KX6frZSl8vLenV2vlQXxS9iNrjI</recordid><startdate>201604</startdate><enddate>201604</enddate><creator>Pinto-Junior, Vanir R.</creator><creator>Correia, Jorge L. A.</creator><creator>Pereira, Ronniery I.</creator><creator>Pereira-Junior, Francisco N.</creator><creator>Santiago, Mayara Q.</creator><creator>Osterne, Vinicius J. S.</creator><creator>Madeira, Juliana C.</creator><creator>Cajazeiras, João B.</creator><creator>Nagano, Celso S.</creator><creator>Delatorre, Plinio</creator><creator>Assreuy, Ana M. S.</creator><creator>Nascimento, Kyria S.</creator><creator>Cavada, Benildo S.</creator><general>Blackwell Publishing Ltd</general><general>Wiley Subscription Services, Inc</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QP</scope><scope>7QQ</scope><scope>7SE</scope><scope>7SR</scope><scope>7TA</scope><scope>7TK</scope><scope>7TM</scope><scope>8BQ</scope><scope>8FD</scope><scope>F28</scope><scope>FR3</scope><scope>H8G</scope><scope>JG9</scope><scope>P64</scope><scope>7X8</scope><scope>7U5</scope><scope>L7M</scope></search><sort><creationdate>201604</creationdate><title>Purification and molecular characterization of a novel mannose-specific lectin from Dioclea reflexa hook seeds with inflammatory activity</title><author>Pinto-Junior, Vanir R. ; Correia, Jorge L. A. ; Pereira, Ronniery I. ; Pereira-Junior, Francisco N. ; Santiago, Mayara Q. ; Osterne, Vinicius J. S. ; Madeira, Juliana C. ; Cajazeiras, João B. ; Nagano, Celso S. ; Delatorre, Plinio ; Assreuy, Ana M. 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A.</au><au>Pereira, Ronniery I.</au><au>Pereira-Junior, Francisco N.</au><au>Santiago, Mayara Q.</au><au>Osterne, Vinicius J. S.</au><au>Madeira, Juliana C.</au><au>Cajazeiras, João B.</au><au>Nagano, Celso S.</au><au>Delatorre, Plinio</au><au>Assreuy, Ana M. S.</au><au>Nascimento, Kyria S.</au><au>Cavada, Benildo S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Purification and molecular characterization of a novel mannose-specific lectin from Dioclea reflexa hook seeds with inflammatory activity</atitle><jtitle>Journal of molecular recognition</jtitle><addtitle>J. Mol. Recognit</addtitle><date>2016-04</date><risdate>2016</risdate><volume>29</volume><issue>4</issue><spage>134</spage><epage>141</epage><pages>134-141</pages><issn>0952-3499</issn><eissn>1099-1352</eissn><abstract>A novel lectin present in Dioclea reflexa seeds (DrfL) was discovered and described in this study. DrfL was purified in a single step by affinity chromatography in a Sephadex G‐50 column. The lectin strongly agglutinated rabbit erythrocytes and was inhibited by α‐methyl‐d‐mannoside, d‐mannose, and d‐glucose. The hemagglutinating activity of DrfL is optimum at pH 5.0–7.0, stable up to 50 °C, and dependent on divalent cations. Similar to other lectins of the subtribe Diocleinae, the analysis by mass spectrometry indicated that DrfL has three chains (α, β, and γ) with masses of 25 562, 12 874, and 12 706 Da, respectively, with no disulfide bonds or glycosylation. DrfL showed inflammatory activity in the paw edema model and exhibited low cytotoxicity against Artemia sp. Copyright © 2015 John Wiley & Sons, Ltd.
A new mannose‐specific lectin from Dioclea reflexa Hook seeds (DrfL) was purified and partially characterized. DrfL toxic effect was evaluated against Artemia sp. DrfL elicits acute inflammation via lectin domain mediated by nitric oxide.</abstract><cop>England</cop><pub>Blackwell Publishing Ltd</pub><pmid>26464029</pmid><doi>10.1002/jmr.2512</doi><tpages>8</tpages></addata></record> |
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| subjects | Animals Artemia Chromatography, Affinity Dioclea - chemistry Dioclea reflexa Edema Edema - chemically induced Erythrocytes Erythrocytes - drug effects ESI mass spectrometry Hemagglutination - drug effects Hooks Inflammation Mediators - isolation & purification Inflammation Mediators - pharmacology inflammatory activity lectin Lectins Mannose - pharmacology Mice Plant Lectins - chemistry Plant Lectins - isolation & purification Plant Lectins - pharmacology Protein Structure, Secondary Rabbits Recognition Seeds Toxic |
| title | Purification and molecular characterization of a novel mannose-specific lectin from Dioclea reflexa hook seeds with inflammatory activity |
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