Biodegradation of the endocrine disrupting chemical o‐phenylenediamine using intracellular enzymes from Citrobacter freundii and its kinetic studies
BACKGROUND: Endocrine disrupting chemicals are widely distributed in environment. o‐Phenylenediamine (OPD), an endocrine disruptor, is widely used in the leather, dyeing and polymer industries. There have been studies on the photocatalytic and biological degradation of OPD but there is no single rep...
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| Veröffentlicht in: | Journal of chemical technology and biotechnology (1986) 2016, Vol.91 (1), p.171-183 |
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| container_title | Journal of chemical technology and biotechnology (1986) |
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| creator | Paranji, Saranya Rajasekaran, Muneeswari Ganesan, Sekaran |
| description | BACKGROUND: Endocrine disrupting chemicals are widely distributed in environment. o‐Phenylenediamine (OPD), an endocrine disruptor, is widely used in the leather, dyeing and polymer industries. There have been studies on the photocatalytic and biological degradation of OPD but there is no single report on the enzymatic degradation of OPD. RESULTS: In the present investigation, purified mixed intracellular enzymes (MIE) were used for the degradation of aqueous OPD from Citrobacter freundii, a marine bacterium. The degradation rate of OPD (concentration, 333 ppm) by MIE was achieved with maximum removal of 93%. The degrading efficiency using MIE was enhanced by Zn²⁺ to about 95.87%. The degradation of OPD using MIE of C. freundii followed pseudo‐second‐order rate kinetics. Fourier transform infrared spectroscopy, ultraviolet–visible spectroscopy, fluorescence spectroscopy, high‐performance liquid chromatography and cyclic voltammetry were used to characterise the degradation of OPD by enzymatic treatment. Nuclear magnetic resonance spectroscopy and gas chromatography–mass spectrometry were used for confirmation of the end product of OPD in enzymatic treatment. CONCLUSION: The endocrine disruptor OPD was degraded into pyruvic acid, a non‐toxic end product, using MIE. © 2014 Society of Chemical Industry |
| doi_str_mv | 10.1002/jctb.4558 |
| format | Article |
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There have been studies on the photocatalytic and biological degradation of OPD but there is no single report on the enzymatic degradation of OPD. RESULTS: In the present investigation, purified mixed intracellular enzymes (MIE) were used for the degradation of aqueous OPD from Citrobacter freundii, a marine bacterium. The degradation rate of OPD (concentration, 333 ppm) by MIE was achieved with maximum removal of 93%. The degrading efficiency using MIE was enhanced by Zn²⁺ to about 95.87%. The degradation of OPD using MIE of C. freundii followed pseudo‐second‐order rate kinetics. Fourier transform infrared spectroscopy, ultraviolet–visible spectroscopy, fluorescence spectroscopy, high‐performance liquid chromatography and cyclic voltammetry were used to characterise the degradation of OPD by enzymatic treatment. Nuclear magnetic resonance spectroscopy and gas chromatography–mass spectrometry were used for confirmation of the end product of OPD in enzymatic treatment. CONCLUSION: The endocrine disruptor OPD was degraded into pyruvic acid, a non‐toxic end product, using MIE. © 2014 Society of Chemical Industry</description><identifier>ISSN: 0268-2575</identifier><identifier>EISSN: 1097-4660</identifier><identifier>DOI: 10.1002/jctb.4558</identifier><language>eng</language><publisher>Chichester, UK: John Wiley & Sons, Ltd</publisher><subject>bacteria ; biodegradation ; Citrobacter ; Citrobacter freundii ; Degradation ; Disrupting ; dyeing ; Endocrine disruptors ; endocrine-disrupting chemicals ; enzymatic treatment ; Enzymes ; Fluorescence ; fluorescence emission spectroscopy ; Fourier transform infrared spectroscopy ; gas chromatography-mass spectrometry ; High performance liquid chromatography ; industry ; intracellular enzymes ; leather ; nuclear magnetic resonance spectroscopy ; o-phenylenediamine ; polymers ; pyruvic acid ; Spectroscopy ; ultraviolet-visible spectroscopy ; zinc</subject><ispartof>Journal of chemical technology and biotechnology (1986), 2016, Vol.91 (1), p.171-183</ispartof><rights>2014 Society of Chemical Industry</rights><rights>2016 Society of Chemical Industry</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4628-ac82e5a34eb75212b262c9d931ee92fb085f1af076b9cae5bc0895b6539af2093</citedby><cites>FETCH-LOGICAL-c4628-ac82e5a34eb75212b262c9d931ee92fb085f1af076b9cae5bc0895b6539af2093</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjctb.4558$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjctb.4558$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,777,781,1412,4010,27904,27905,27906,45555,45556</link.rule.ids></links><search><creatorcontrib>Paranji, Saranya</creatorcontrib><creatorcontrib>Rajasekaran, Muneeswari</creatorcontrib><creatorcontrib>Ganesan, Sekaran</creatorcontrib><title>Biodegradation of the endocrine disrupting chemical o‐phenylenediamine using intracellular enzymes from Citrobacter freundii and its kinetic studies</title><title>Journal of chemical technology and biotechnology (1986)</title><addtitle>J. Chem. Technol. Biotechnol</addtitle><description>BACKGROUND: Endocrine disrupting chemicals are widely distributed in environment. o‐Phenylenediamine (OPD), an endocrine disruptor, is widely used in the leather, dyeing and polymer industries. There have been studies on the photocatalytic and biological degradation of OPD but there is no single report on the enzymatic degradation of OPD. RESULTS: In the present investigation, purified mixed intracellular enzymes (MIE) were used for the degradation of aqueous OPD from Citrobacter freundii, a marine bacterium. The degradation rate of OPD (concentration, 333 ppm) by MIE was achieved with maximum removal of 93%. The degrading efficiency using MIE was enhanced by Zn²⁺ to about 95.87%. The degradation of OPD using MIE of C. freundii followed pseudo‐second‐order rate kinetics. Fourier transform infrared spectroscopy, ultraviolet–visible spectroscopy, fluorescence spectroscopy, high‐performance liquid chromatography and cyclic voltammetry were used to characterise the degradation of OPD by enzymatic treatment. Nuclear magnetic resonance spectroscopy and gas chromatography–mass spectrometry were used for confirmation of the end product of OPD in enzymatic treatment. CONCLUSION: The endocrine disruptor OPD was degraded into pyruvic acid, a non‐toxic end product, using MIE. © 2014 Society of Chemical Industry</description><subject>bacteria</subject><subject>biodegradation</subject><subject>Citrobacter</subject><subject>Citrobacter freundii</subject><subject>Degradation</subject><subject>Disrupting</subject><subject>dyeing</subject><subject>Endocrine disruptors</subject><subject>endocrine-disrupting chemicals</subject><subject>enzymatic treatment</subject><subject>Enzymes</subject><subject>Fluorescence</subject><subject>fluorescence emission spectroscopy</subject><subject>Fourier transform infrared spectroscopy</subject><subject>gas chromatography-mass spectrometry</subject><subject>High performance liquid chromatography</subject><subject>industry</subject><subject>intracellular enzymes</subject><subject>leather</subject><subject>nuclear magnetic resonance spectroscopy</subject><subject>o-phenylenediamine</subject><subject>polymers</subject><subject>pyruvic acid</subject><subject>Spectroscopy</subject><subject>ultraviolet-visible spectroscopy</subject><subject>zinc</subject><issn>0268-2575</issn><issn>1097-4660</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><recordid>eNqF0sFu1DAQBuAIgcRSOPAEWOICh7S2EzvxkY2ggLbLgVYcLceZ7Hqb2FvbEd2eeAROPCBPgsNWHJAQJ0vWN79mPM6y5wSfEozp2U7H9rRkrH6QLQgWVV5yjh9mC0x5nVNWscfZkxB2GGNeU77IfiyN62DjVaeicRa5HsUtILCd095YQJ0JftpHYzdIb2E0Wg3I_fz2fb8FexjAQmfUOMMpzMbY6JWGYZgG5VPM3WGEgHrvRtSY6F2rdASfLmCynTFI2Q6ZGNB1iohGoxCnzkB4mj3q1RDg2f15kl29e3vZvM9Xn84_NG9WuS45rXOlawpMFSW0FaOEtpRTLTpREABB-xbXrCeqxxVvhVbAWo1rwVrOCqF6ikVxkr065u69u5kgRDmaMLevLLgpSFJjXApaVOz_tKo45yWvSaIv_6I7N3mbBkmKzZ2K34Gvj0p7F4KHXu69GZU_SILlvEw5L1POy0z27Gi_mgEO_4byY3O5vK_IjxUmRLj9U6H8teRVmkd-WZ_L5qK5WBXLtVwn_-Loe-Wk2ngT5NVniglPX6VMD8CLX8s8vaw</recordid><startdate>2016</startdate><enddate>2016</enddate><creator>Paranji, Saranya</creator><creator>Rajasekaran, Muneeswari</creator><creator>Ganesan, Sekaran</creator><general>John Wiley & Sons, Ltd</general><general>Wiley Subscription Services, Inc</general><scope>FBQ</scope><scope>BSCLL</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7QR</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7T7</scope><scope>7TA</scope><scope>7TB</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>7QL</scope></search><sort><creationdate>2016</creationdate><title>Biodegradation of the endocrine disrupting chemical o‐phenylenediamine using intracellular enzymes from Citrobacter freundii and its kinetic studies</title><author>Paranji, Saranya ; Rajasekaran, Muneeswari ; Ganesan, Sekaran</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4628-ac82e5a34eb75212b262c9d931ee92fb085f1af076b9cae5bc0895b6539af2093</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>bacteria</topic><topic>biodegradation</topic><topic>Citrobacter</topic><topic>Citrobacter freundii</topic><topic>Degradation</topic><topic>Disrupting</topic><topic>dyeing</topic><topic>Endocrine disruptors</topic><topic>endocrine-disrupting chemicals</topic><topic>enzymatic treatment</topic><topic>Enzymes</topic><topic>Fluorescence</topic><topic>fluorescence emission spectroscopy</topic><topic>Fourier transform infrared spectroscopy</topic><topic>gas chromatography-mass spectrometry</topic><topic>High performance liquid chromatography</topic><topic>industry</topic><topic>intracellular enzymes</topic><topic>leather</topic><topic>nuclear magnetic resonance spectroscopy</topic><topic>o-phenylenediamine</topic><topic>polymers</topic><topic>pyruvic acid</topic><topic>Spectroscopy</topic><topic>ultraviolet-visible spectroscopy</topic><topic>zinc</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Paranji, Saranya</creatorcontrib><creatorcontrib>Rajasekaran, Muneeswari</creatorcontrib><creatorcontrib>Ganesan, Sekaran</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><jtitle>Journal of chemical technology and biotechnology (1986)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Paranji, Saranya</au><au>Rajasekaran, Muneeswari</au><au>Ganesan, Sekaran</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Biodegradation of the endocrine disrupting chemical o‐phenylenediamine using intracellular enzymes from Citrobacter freundii and its kinetic studies</atitle><jtitle>Journal of chemical technology and biotechnology (1986)</jtitle><addtitle>J. Chem. Technol. Biotechnol</addtitle><date>2016</date><risdate>2016</risdate><volume>91</volume><issue>1</issue><spage>171</spage><epage>183</epage><pages>171-183</pages><issn>0268-2575</issn><eissn>1097-4660</eissn><abstract>BACKGROUND: Endocrine disrupting chemicals are widely distributed in environment. o‐Phenylenediamine (OPD), an endocrine disruptor, is widely used in the leather, dyeing and polymer industries. There have been studies on the photocatalytic and biological degradation of OPD but there is no single report on the enzymatic degradation of OPD. RESULTS: In the present investigation, purified mixed intracellular enzymes (MIE) were used for the degradation of aqueous OPD from Citrobacter freundii, a marine bacterium. The degradation rate of OPD (concentration, 333 ppm) by MIE was achieved with maximum removal of 93%. The degrading efficiency using MIE was enhanced by Zn²⁺ to about 95.87%. The degradation of OPD using MIE of C. freundii followed pseudo‐second‐order rate kinetics. Fourier transform infrared spectroscopy, ultraviolet–visible spectroscopy, fluorescence spectroscopy, high‐performance liquid chromatography and cyclic voltammetry were used to characterise the degradation of OPD by enzymatic treatment. Nuclear magnetic resonance spectroscopy and gas chromatography–mass spectrometry were used for confirmation of the end product of OPD in enzymatic treatment. CONCLUSION: The endocrine disruptor OPD was degraded into pyruvic acid, a non‐toxic end product, using MIE. © 2014 Society of Chemical Industry</abstract><cop>Chichester, UK</cop><pub>John Wiley & Sons, Ltd</pub><doi>10.1002/jctb.4558</doi><tpages>13</tpages></addata></record> |
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| ispartof | Journal of chemical technology and biotechnology (1986), 2016, Vol.91 (1), p.171-183 |
| issn | 0268-2575 1097-4660 |
| language | eng |
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| source | Wiley Online Library Journals Frontfile Complete |
| subjects | bacteria biodegradation Citrobacter Citrobacter freundii Degradation Disrupting dyeing Endocrine disruptors endocrine-disrupting chemicals enzymatic treatment Enzymes Fluorescence fluorescence emission spectroscopy Fourier transform infrared spectroscopy gas chromatography-mass spectrometry High performance liquid chromatography industry intracellular enzymes leather nuclear magnetic resonance spectroscopy o-phenylenediamine polymers pyruvic acid Spectroscopy ultraviolet-visible spectroscopy zinc |
| title | Biodegradation of the endocrine disrupting chemical o‐phenylenediamine using intracellular enzymes from Citrobacter freundii and its kinetic studies |
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