Detection of Different DNA Animal Species in Commercial Candy Products
Candy products are consumed all across the world, but there is not much information about their composition. In this study we have used a DNA‐based approach for determining the animal species occurring in 40 commercial candies of different types. We extracted DNA and performed PCR amplification, clo...
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| Veröffentlicht in: | Journal of food science 2016-03, Vol.81 (3), p.T801-T809 |
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| container_title | Journal of food science |
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| creator | Muñoz-Colmenero, Marta Martínez, Jose Luis Roca, Agustín Garcia-Vazquez, Eva |
| description | Candy products are consumed all across the world, but there is not much information about their composition. In this study we have used a DNA‐based approach for determining the animal species occurring in 40 commercial candies of different types. We extracted DNA and performed PCR amplification, cloning and sequencing for obtaining species‐informative DNA sequences. Eight species were identified including fish (hake and anchovy) in 22% of the products analyzed. Bovine and porcine were the most abundant appearing in 27 samples each one. Most products contained a mixture of species. Marshmallows (7), jelly‐types, and gummies (20) contained a significantly higher number of species than hard candies (9). We demonstrated the presence of DNA animal species in candy product which allow consumers to make choices and prevent allergic reaction. |
| doi_str_mv | 10.1111/1750-3841.13225 |
| format | Article |
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In this study we have used a DNA‐based approach for determining the animal species occurring in 40 commercial candies of different types. We extracted DNA and performed PCR amplification, cloning and sequencing for obtaining species‐informative DNA sequences. Eight species were identified including fish (hake and anchovy) in 22% of the products analyzed. Bovine and porcine were the most abundant appearing in 27 samples each one. Most products contained a mixture of species. Marshmallows (7), jelly‐types, and gummies (20) contained a significantly higher number of species than hard candies (9). 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In this study we have used a DNA‐based approach for determining the animal species occurring in 40 commercial candies of different types. We extracted DNA and performed PCR amplification, cloning and sequencing for obtaining species‐informative DNA sequences. Eight species were identified including fish (hake and anchovy) in 22% of the products analyzed. Bovine and porcine were the most abundant appearing in 27 samples each one. Most products contained a mixture of species. Marshmallows (7), jelly‐types, and gummies (20) contained a significantly higher number of species than hard candies (9). 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| source | Wiley-Blackwell Journals; MEDLINE |
| subjects | Allergens - analysis Anchovies animal species Animals Base Sequence Buffaloes Candy Candy - analysis Cattle Cloning Confectionery Consumption Deoxyribonucleic acid DNA DNA - analysis DNA markers Fish Fishes Food allergies Food Hypersensitivity - prevention & control Food science gelatin Gene sequencing Humans PCR cloning Polymerase Chain Reaction Swine |
| title | Detection of Different DNA Animal Species in Commercial Candy Products |
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