Fine structure of tooth enamel in the yellowing human teeth: SEM and HRTEM studies
ABSTRACT Objective: The aim of this study was to clarify an influence of the fine structure of human tooth enamel to the yellowing teeth. Materials and methods: Sound maxillary first premolars of 15–50‐year‐old females that were extracted for the orthodontic treatment were used as the test samples....
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| Veröffentlicht in: | Microscopy research and technique 2016-01, Vol.79 (1), p.14-22 |
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| creator | Wakasa, Masanobu Nakanishi, Kuniyuki Manago, Kenji Isobe, Tsutomu Eshita, Yoshiyuki Okamoto, Masayuki Isshiki, Toshiyuki |
| description | ABSTRACT
Objective: The aim of this study was to clarify an influence of the fine structure of human tooth enamel to the yellowing teeth. Materials and methods: Sound maxillary first premolars of 15–50‐year‐old females that were extracted for the orthodontic treatment were used as the test samples. The tooth enamel sections of these teeth that prepared by ion polishing were observed by scanning electron microscopy (SEM). Furthermore, the fine structure of substance filling the inter‐rod spaces was analyzed by high resolution transmission electron microscopy (HRTEM). Results: In white tooth, the inter‐rod spaces were observed at the width of about 0.1 μm, while in yellow tooth, the inter‐rod spaces were not clearly observed by SEM. HRTEM observations revealed for the first time that the inter‐rod spaces were filled with fine particles of poorly crystallized hydroxyapatite in the yellow tooth. In yellow tooth, it was considered that the color of the inner dentin was recognized due to the decrease of light scattering by filling the tooth enamel inter‐rod spaces. The generation of particles in the tooth enamel inter‐rod spaces was considered to be caused by the long‐time progression of calcification. Conclusions: These results suggested that the change in fine structure, filling in inter‐rod spaces of tooth enamel, was related to progression of calcification in the inter‐rod spaces with advancing age and one of the factors of yellowness of human tooth. Microsc. Res. Tech. 79:14–22, 2016. © 2015 Wiley Periodicals, Inc. |
| doi_str_mv | 10.1002/jemt.22600 |
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Objective: The aim of this study was to clarify an influence of the fine structure of human tooth enamel to the yellowing teeth. Materials and methods: Sound maxillary first premolars of 15–50‐year‐old females that were extracted for the orthodontic treatment were used as the test samples. The tooth enamel sections of these teeth that prepared by ion polishing were observed by scanning electron microscopy (SEM). Furthermore, the fine structure of substance filling the inter‐rod spaces was analyzed by high resolution transmission electron microscopy (HRTEM). Results: In white tooth, the inter‐rod spaces were observed at the width of about 0.1 μm, while in yellow tooth, the inter‐rod spaces were not clearly observed by SEM. HRTEM observations revealed for the first time that the inter‐rod spaces were filled with fine particles of poorly crystallized hydroxyapatite in the yellow tooth. In yellow tooth, it was considered that the color of the inner dentin was recognized due to the decrease of light scattering by filling the tooth enamel inter‐rod spaces. The generation of particles in the tooth enamel inter‐rod spaces was considered to be caused by the long‐time progression of calcification. Conclusions: These results suggested that the change in fine structure, filling in inter‐rod spaces of tooth enamel, was related to progression of calcification in the inter‐rod spaces with advancing age and one of the factors of yellowness of human tooth. Microsc. Res. Tech. 79:14–22, 2016. © 2015 Wiley Periodicals, Inc.</description><identifier>ISSN: 1059-910X</identifier><identifier>EISSN: 1097-0029</identifier><identifier>DOI: 10.1002/jemt.22600</identifier><identifier>PMID: 26768788</identifier><identifier>CODEN: MRTEEO</identifier><language>eng</language><publisher>United States: Blackwell Publishing Ltd</publisher><subject>Adolescent ; Adult ; Calcification ; Dental Enamel - ultrastructure ; Dentin - ultrastructure ; Female ; Fine structure ; Human ; Humans ; hydroxyapatite ; In Vitro Techniques ; inter-rod spaces ; ion polishing ; Microscopy, Electron, Scanning ; Microscopy, Electron, Transmission ; Middle Aged ; Pigmentation ; Progressions ; Scanning electron microscopy ; Surface Properties ; Teeth ; Tooth enamels ; Yellowing ; Young Adult</subject><ispartof>Microscopy research and technique, 2016-01, Vol.79 (1), p.14-22</ispartof><rights>2015 Wiley Periodicals, Inc.</rights><rights>2016 Wiley Periodicals, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4920-d27ad79457c7013fce46f91df970f31b58dbc437c6473db4bd7f8d8621be6e283</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjemt.22600$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjemt.22600$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26768788$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wakasa, Masanobu</creatorcontrib><creatorcontrib>Nakanishi, Kuniyuki</creatorcontrib><creatorcontrib>Manago, Kenji</creatorcontrib><creatorcontrib>Isobe, Tsutomu</creatorcontrib><creatorcontrib>Eshita, Yoshiyuki</creatorcontrib><creatorcontrib>Okamoto, Masayuki</creatorcontrib><creatorcontrib>Isshiki, Toshiyuki</creatorcontrib><title>Fine structure of tooth enamel in the yellowing human teeth: SEM and HRTEM studies</title><title>Microscopy research and technique</title><addtitle>Microsc. Res. Tech</addtitle><description>ABSTRACT
Objective: The aim of this study was to clarify an influence of the fine structure of human tooth enamel to the yellowing teeth. Materials and methods: Sound maxillary first premolars of 15–50‐year‐old females that were extracted for the orthodontic treatment were used as the test samples. The tooth enamel sections of these teeth that prepared by ion polishing were observed by scanning electron microscopy (SEM). Furthermore, the fine structure of substance filling the inter‐rod spaces was analyzed by high resolution transmission electron microscopy (HRTEM). Results: In white tooth, the inter‐rod spaces were observed at the width of about 0.1 μm, while in yellow tooth, the inter‐rod spaces were not clearly observed by SEM. HRTEM observations revealed for the first time that the inter‐rod spaces were filled with fine particles of poorly crystallized hydroxyapatite in the yellow tooth. In yellow tooth, it was considered that the color of the inner dentin was recognized due to the decrease of light scattering by filling the tooth enamel inter‐rod spaces. The generation of particles in the tooth enamel inter‐rod spaces was considered to be caused by the long‐time progression of calcification. Conclusions: These results suggested that the change in fine structure, filling in inter‐rod spaces of tooth enamel, was related to progression of calcification in the inter‐rod spaces with advancing age and one of the factors of yellowness of human tooth. Microsc. Res. Tech. 79:14–22, 2016. © 2015 Wiley Periodicals, Inc.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Calcification</subject><subject>Dental Enamel - ultrastructure</subject><subject>Dentin - ultrastructure</subject><subject>Female</subject><subject>Fine structure</subject><subject>Human</subject><subject>Humans</subject><subject>hydroxyapatite</subject><subject>In Vitro Techniques</subject><subject>inter-rod spaces</subject><subject>ion polishing</subject><subject>Microscopy, Electron, Scanning</subject><subject>Microscopy, Electron, Transmission</subject><subject>Middle Aged</subject><subject>Pigmentation</subject><subject>Progressions</subject><subject>Scanning electron microscopy</subject><subject>Surface Properties</subject><subject>Teeth</subject><subject>Tooth enamels</subject><subject>Yellowing</subject><subject>Young Adult</subject><issn>1059-910X</issn><issn>1097-0029</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU1v00AQhi0EoqVw4Qeglbhwcbvfs8uNRklK1YLUpqK3le0dEwd_FO9aJf-eTVN64AKneTXzvKMZvVn2ltFjRik_2WAXjznXlD7LDhm1kKeufb7TyuaW0duD7FUIG0oZU0y-zA64Bm3AmMPsatH0SEIcpypOI5KhJnEY4ppgX3TYkqYncY1ki2073Df9d7KeuiL1EOP6I7meX5Ki9-TsapVUiJNvMLzOXtRFG_DNYz3Kbhbz1ewsv_i6_Dz7dJFX0nKaew6FBysVVECZqCuUurbM1xZoLVipjC8rKaDSEoQvZemhNt5ozkrUyI04yj7s996Nw88JQ3RdE6p0aNHjMAXHDKUSQGr-bxQMVUwB_MdW0NRYwZRI6Pu_0M0wjX36OVEKFE9H7qh3j9RUdujd3dh0xbh1fzJIANsD902L26c5o26Xrtul6x7Sdefzy9WDSp5872lCxF9PnmL84TQIUO7bl6UDupzdnpqlW4jfvEKjeg</recordid><startdate>201601</startdate><enddate>201601</enddate><creator>Wakasa, Masanobu</creator><creator>Nakanishi, Kuniyuki</creator><creator>Manago, Kenji</creator><creator>Isobe, Tsutomu</creator><creator>Eshita, Yoshiyuki</creator><creator>Okamoto, Masayuki</creator><creator>Isshiki, Toshiyuki</creator><general>Blackwell Publishing Ltd</general><general>Wiley Subscription Services, Inc</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7QF</scope><scope>7QO</scope><scope>7QP</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7SS</scope><scope>7TA</scope><scope>7TB</scope><scope>7U5</scope><scope>7U7</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>JG9</scope><scope>JQ2</scope><scope>K9.</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>201601</creationdate><title>Fine structure of tooth enamel in the yellowing human teeth: SEM and HRTEM studies</title><author>Wakasa, Masanobu ; Nakanishi, Kuniyuki ; Manago, Kenji ; Isobe, Tsutomu ; Eshita, Yoshiyuki ; Okamoto, Masayuki ; Isshiki, Toshiyuki</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4920-d27ad79457c7013fce46f91df970f31b58dbc437c6473db4bd7f8d8621be6e283</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Calcification</topic><topic>Dental Enamel - ultrastructure</topic><topic>Dentin - ultrastructure</topic><topic>Female</topic><topic>Fine structure</topic><topic>Human</topic><topic>Humans</topic><topic>hydroxyapatite</topic><topic>In Vitro Techniques</topic><topic>inter-rod spaces</topic><topic>ion polishing</topic><topic>Microscopy, Electron, Scanning</topic><topic>Microscopy, Electron, Transmission</topic><topic>Middle Aged</topic><topic>Pigmentation</topic><topic>Progressions</topic><topic>Scanning electron microscopy</topic><topic>Surface Properties</topic><topic>Teeth</topic><topic>Tooth enamels</topic><topic>Yellowing</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wakasa, Masanobu</creatorcontrib><creatorcontrib>Nakanishi, Kuniyuki</creatorcontrib><creatorcontrib>Manago, Kenji</creatorcontrib><creatorcontrib>Isobe, Tsutomu</creatorcontrib><creatorcontrib>Eshita, Yoshiyuki</creatorcontrib><creatorcontrib>Okamoto, Masayuki</creatorcontrib><creatorcontrib>Isshiki, Toshiyuki</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Toxicology Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Microscopy research and technique</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wakasa, Masanobu</au><au>Nakanishi, Kuniyuki</au><au>Manago, Kenji</au><au>Isobe, Tsutomu</au><au>Eshita, Yoshiyuki</au><au>Okamoto, Masayuki</au><au>Isshiki, Toshiyuki</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Fine structure of tooth enamel in the yellowing human teeth: SEM and HRTEM studies</atitle><jtitle>Microscopy research and technique</jtitle><addtitle>Microsc. Res. Tech</addtitle><date>2016-01</date><risdate>2016</risdate><volume>79</volume><issue>1</issue><spage>14</spage><epage>22</epage><pages>14-22</pages><issn>1059-910X</issn><eissn>1097-0029</eissn><coden>MRTEEO</coden><abstract>ABSTRACT
Objective: The aim of this study was to clarify an influence of the fine structure of human tooth enamel to the yellowing teeth. Materials and methods: Sound maxillary first premolars of 15–50‐year‐old females that were extracted for the orthodontic treatment were used as the test samples. The tooth enamel sections of these teeth that prepared by ion polishing were observed by scanning electron microscopy (SEM). Furthermore, the fine structure of substance filling the inter‐rod spaces was analyzed by high resolution transmission electron microscopy (HRTEM). Results: In white tooth, the inter‐rod spaces were observed at the width of about 0.1 μm, while in yellow tooth, the inter‐rod spaces were not clearly observed by SEM. HRTEM observations revealed for the first time that the inter‐rod spaces were filled with fine particles of poorly crystallized hydroxyapatite in the yellow tooth. In yellow tooth, it was considered that the color of the inner dentin was recognized due to the decrease of light scattering by filling the tooth enamel inter‐rod spaces. The generation of particles in the tooth enamel inter‐rod spaces was considered to be caused by the long‐time progression of calcification. Conclusions: These results suggested that the change in fine structure, filling in inter‐rod spaces of tooth enamel, was related to progression of calcification in the inter‐rod spaces with advancing age and one of the factors of yellowness of human tooth. Microsc. Res. Tech. 79:14–22, 2016. © 2015 Wiley Periodicals, Inc.</abstract><cop>United States</cop><pub>Blackwell Publishing Ltd</pub><pmid>26768788</pmid><doi>10.1002/jemt.22600</doi><tpages>9</tpages></addata></record> |
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| subjects | Adolescent Adult Calcification Dental Enamel - ultrastructure Dentin - ultrastructure Female Fine structure Human Humans hydroxyapatite In Vitro Techniques inter-rod spaces ion polishing Microscopy, Electron, Scanning Microscopy, Electron, Transmission Middle Aged Pigmentation Progressions Scanning electron microscopy Surface Properties Teeth Tooth enamels Yellowing Young Adult |
| title | Fine structure of tooth enamel in the yellowing human teeth: SEM and HRTEM studies |
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