HPLC-MS with Glass Chips Featuring Monolithically Integrated Electrospray Emitters of Different Geometries

HPLC-MS with Glass Chips Featuring Monolithically Integrated Electrospray Emitters of Different Geometries

We present and evaluate an approach for coupling liquid chromatography in glass chips with mass spectrometry via fully integrated electrospray emitters. We developed an instrumental platform which allows a robust and reproducible operation of high performance chip chromatography coupled to mass spec...

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Veröffentlicht in:Analytical chemistry (Washington) 2016-03, Vol.88 (5), p.2856-2863
Hauptverfasser: Lotter, Carsten, Heiland, Josef J, Thurmann, Sebastian, Mauritz, Laura, Belder, Detlev
Format: Artikel
Sprache:eng
Schlagworte:
Chips
Chromatography
Emitters
Flow rate
Geometry
Glass
Joining
Mass spectrometry
Nanostructure
Platforms
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container_issue 5
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container_title Analytical chemistry (Washington)
container_volume 88
creator Lotter, Carsten
Heiland, Josef J
Thurmann, Sebastian
Mauritz, Laura
Belder, Detlev
description We present and evaluate an approach for coupling liquid chromatography in glass chips with mass spectrometry via fully integrated electrospray emitters. We developed an instrumental platform which allows a robust and reproducible operation of high performance chip chromatography coupled to mass spectrometry. A comparison of differently shaped emitters, from flat over edged to pulled geometries, revealed that all types performed equally well for typical nano-HPLC flow rates. At very low flow rates below 50 nL·min–1 very sharp, pulled nanospray emitters turned out to be mandatory for the generation of a stable electrospray.
doi_str_mv 10.1021/acs.analchem.5b04583
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source ACS_美国化学学会期刊（与NSTL共建）
subjects Chips
Chromatography
Emitters
Flow rate
Geometry
Glass
Joining
Mass spectrometry
Nanostructure
Platforms
title HPLC-MS with Glass Chips Featuring Monolithically Integrated Electrospray Emitters of Different Geometries
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