Fabrication of Amperometric Glucose Sensor Using Glucose Oxidase-Cellulose Nanofiber Aqueous Solution
Cellulose nanofiber aqueous solution, which remained virtually transparent for more than one week, was prepared by using the clear upper layer of diluted cellulose nanofiber solution produced by wet jet milling. Glucose oxidase (GOx) was easily dissolved in this solution and GOx-immobilized electrod...
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| Veröffentlicht in: | Analytical Sciences 2015/11/10, Vol.31(11), pp.1111-1114 |
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| creator | YASUZAWA, Mikito OMURA, Yuya HIURA, Kentaro LI, Jiang FUCHIWAKI, Yusuke TANAKA, Masato |
| description | Cellulose nanofiber aqueous solution, which remained virtually transparent for more than one week, was prepared by using the clear upper layer of diluted cellulose nanofiber solution produced by wet jet milling. Glucose oxidase (GOx) was easily dissolved in this solution and GOx-immobilized electrode was easily fabricated by simple repetitious drops of GOx-cellulose solution on the surface of a platinum-iridium electrode. Glucose sensor properties of the obtained electrodes were examined in phosphate buffer solution of pH 7.4 at 40°C. The obtained electrode provided a glucose sensor response with significantly high response speed and good linear relationship between glucose concentration and response current. After an initial decrease of response sensitivity for a few days, relatively constant sensitivity was obtained for about 20 days. Nevertheless, the influence of electroactive compounds such as ascorbic acid, uric acid and acetoaminophen were not negletable. |
| doi_str_mv | 10.2116/analsci.31.1111 |
| format | Article |
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Glucose oxidase (GOx) was easily dissolved in this solution and GOx-immobilized electrode was easily fabricated by simple repetitious drops of GOx-cellulose solution on the surface of a platinum-iridium electrode. Glucose sensor properties of the obtained electrodes were examined in phosphate buffer solution of pH 7.4 at 40°C. The obtained electrode provided a glucose sensor response with significantly high response speed and good linear relationship between glucose concentration and response current. After an initial decrease of response sensitivity for a few days, relatively constant sensitivity was obtained for about 20 days. Nevertheless, the influence of electroactive compounds such as ascorbic acid, uric acid and acetoaminophen were not negletable.</description><identifier>ISSN: 0910-6340</identifier><identifier>EISSN: 1348-2246</identifier><identifier>DOI: 10.2116/analsci.31.1111</identifier><identifier>PMID: 26561252</identifier><language>eng</language><publisher>Singapore: The Japan Society for Analytical Chemistry</publisher><subject>Analytical Chemistry ; Aqueous solutions ; Aspergillus niger - enzymology ; Biosensing Techniques - methods ; Cellulose ; Cellulose - chemistry ; Cellulose nanofiber ; Chemistry ; Constants ; Electrochemistry ; Electrodes ; enzyme immobilization ; Enzymes, Immobilized - chemistry ; Enzymes, Immobilized - metabolism ; Glucose ; Glucose - analysis ; Glucose - chemistry ; Glucose Oxidase - chemistry ; Glucose Oxidase - metabolism ; glucose sensor ; Nanofibers - chemistry ; Nanostructure ; Rapid Communications ; Sensors ; Solutions ; Uric acid ; Water - chemistry</subject><ispartof>Analytical Sciences, 2015/11/10, Vol.31(11), pp.1111-1114</ispartof><rights>2015 by The Japan Society for Analytical Chemistry</rights><rights>The Japan Society for Analytical Chemistry 2015</rights><rights>Copyright Japan Science and Technology Agency 2015</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c718t-593a3861543710903c7f39760ad15817b32114534a36494b5593ff9e336dfa173</citedby><cites>FETCH-LOGICAL-c718t-593a3861543710903c7f39760ad15817b32114534a36494b5593ff9e336dfa173</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.2116/analsci.31.1111$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.2116/analsci.31.1111$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>315,781,785,1884,27928,27929,41492,42561,51323</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26561252$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>YASUZAWA, Mikito</creatorcontrib><creatorcontrib>OMURA, Yuya</creatorcontrib><creatorcontrib>HIURA, Kentaro</creatorcontrib><creatorcontrib>LI, Jiang</creatorcontrib><creatorcontrib>FUCHIWAKI, Yusuke</creatorcontrib><creatorcontrib>TANAKA, Masato</creatorcontrib><title>Fabrication of Amperometric Glucose Sensor Using Glucose Oxidase-Cellulose Nanofiber Aqueous Solution</title><title>Analytical Sciences</title><addtitle>ANAL. SCI</addtitle><addtitle>Anal Sci</addtitle><description>Cellulose nanofiber aqueous solution, which remained virtually transparent for more than one week, was prepared by using the clear upper layer of diluted cellulose nanofiber solution produced by wet jet milling. Glucose oxidase (GOx) was easily dissolved in this solution and GOx-immobilized electrode was easily fabricated by simple repetitious drops of GOx-cellulose solution on the surface of a platinum-iridium electrode. Glucose sensor properties of the obtained electrodes were examined in phosphate buffer solution of pH 7.4 at 40°C. The obtained electrode provided a glucose sensor response with significantly high response speed and good linear relationship between glucose concentration and response current. After an initial decrease of response sensitivity for a few days, relatively constant sensitivity was obtained for about 20 days. Nevertheless, the influence of electroactive compounds such as ascorbic acid, uric acid and acetoaminophen were not negletable.</description><subject>Analytical Chemistry</subject><subject>Aqueous solutions</subject><subject>Aspergillus niger - enzymology</subject><subject>Biosensing Techniques - methods</subject><subject>Cellulose</subject><subject>Cellulose - chemistry</subject><subject>Cellulose nanofiber</subject><subject>Chemistry</subject><subject>Constants</subject><subject>Electrochemistry</subject><subject>Electrodes</subject><subject>enzyme immobilization</subject><subject>Enzymes, Immobilized - chemistry</subject><subject>Enzymes, Immobilized - metabolism</subject><subject>Glucose</subject><subject>Glucose - analysis</subject><subject>Glucose - chemistry</subject><subject>Glucose Oxidase - chemistry</subject><subject>Glucose Oxidase - metabolism</subject><subject>glucose sensor</subject><subject>Nanofibers - chemistry</subject><subject>Nanostructure</subject><subject>Rapid Communications</subject><subject>Sensors</subject><subject>Solutions</subject><subject>Uric acid</subject><subject>Water - chemistry</subject><issn>0910-6340</issn><issn>1348-2246</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1r3DAQxUVpaTZpz70VQy-9eKPR6MM-LkuSBkJzSHMWslbearGtrWRD-99HZjdLWwjRRTDzmzdPeoR8ArpkAPLSDKZL1i8RlpDPG7IA5FXJGJdvyYLWQEuJnJ6R85R2lAKrGHtPzpgUEphgC-KuTRO9NaMPQxHaYtXvXQy9G3OxuOkmG5IrHtyQQiwekx-2p-L9b78xyZVr13VTN1e-myG0vnGxWP2aXJhS8RC6aVb-QN612aj7eLwvyOP11Y_1t_Lu_uZ2vborrYJqLEWNBisJgqMCWlO0qsVaSWo2ICpQDeZHc4HcoOQ1b0QeaNvaIcpNa0DhBfl60N3HkC2kUfc-2WzQDLMfDRWlnFeCiddRhQh1_scZ_fIfugtTnH8-U0IyBUzNuy8PlI0hpehavY--N_GPBqrnsPQxLI2g57DyxOej7tT0bnPin9PJAD0AKbeGrYt_LX5Rc30Y2aXRbN1J08TR2879yz9Pnbr2p4naDfgEMmS3uQ</recordid><startdate>2015</startdate><enddate>2015</enddate><creator>YASUZAWA, Mikito</creator><creator>OMURA, Yuya</creator><creator>HIURA, Kentaro</creator><creator>LI, Jiang</creator><creator>FUCHIWAKI, Yusuke</creator><creator>TANAKA, Masato</creator><general>The Japan Society for Analytical Chemistry</general><general>Springer Nature Singapore</general><general>Japan Science and Technology Agency</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SE</scope><scope>7SR</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>FR3</scope><scope>H8G</scope><scope>JG9</scope><scope>L7M</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>2015</creationdate><title>Fabrication of Amperometric Glucose Sensor Using Glucose Oxidase-Cellulose Nanofiber Aqueous Solution</title><author>YASUZAWA, Mikito ; 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SCI</stitle><addtitle>Anal Sci</addtitle><date>2015</date><risdate>2015</risdate><volume>31</volume><issue>11</issue><spage>1111</spage><epage>1114</epage><pages>1111-1114</pages><issn>0910-6340</issn><eissn>1348-2246</eissn><abstract>Cellulose nanofiber aqueous solution, which remained virtually transparent for more than one week, was prepared by using the clear upper layer of diluted cellulose nanofiber solution produced by wet jet milling. Glucose oxidase (GOx) was easily dissolved in this solution and GOx-immobilized electrode was easily fabricated by simple repetitious drops of GOx-cellulose solution on the surface of a platinum-iridium electrode. Glucose sensor properties of the obtained electrodes were examined in phosphate buffer solution of pH 7.4 at 40°C. The obtained electrode provided a glucose sensor response with significantly high response speed and good linear relationship between glucose concentration and response current. 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| subjects | Analytical Chemistry Aqueous solutions Aspergillus niger - enzymology Biosensing Techniques - methods Cellulose Cellulose - chemistry Cellulose nanofiber Chemistry Constants Electrochemistry Electrodes enzyme immobilization Enzymes, Immobilized - chemistry Enzymes, Immobilized - metabolism Glucose Glucose - analysis Glucose - chemistry Glucose Oxidase - chemistry Glucose Oxidase - metabolism glucose sensor Nanofibers - chemistry Nanostructure Rapid Communications Sensors Solutions Uric acid Water - chemistry |
| title | Fabrication of Amperometric Glucose Sensor Using Glucose Oxidase-Cellulose Nanofiber Aqueous Solution |
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