Modification of silicon nitride surfaces with GOPES and APTES for antibody immobilization: computational and experimental studies
Chemical modification of silicon nitride (SiN) surfaces by silanization has been widely studied especially with 3-(aminopropyl)triethoxysilane (APTES) and 3-(glycidyloxypropyl) dimethylethoxysilane (GOPES). However few reports performed the experimental and computational studies together. In this st...
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| Veröffentlicht in: | Advances in natural sciences. Nanoscience and nanotechnology 2015-12, Vol.6 (4), p.45006-8 |
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| container_title | Advances in natural sciences. Nanoscience and nanotechnology |
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| creator | To, Thien Dien Nguyen, Anh Tuan Phan, Khoa Nhat Thanh Truong, An Thu Thi Doan, Tin Chanh Duc Dang, Chien Mau |
| description | Chemical modification of silicon nitride (SiN) surfaces by silanization has been widely studied especially with 3-(aminopropyl)triethoxysilane (APTES) and 3-(glycidyloxypropyl) dimethylethoxysilane (GOPES). However few reports performed the experimental and computational studies together. In this study, surface modification of SiN surfaces with GOPES and APTES covalently bound with glutaraldehyde (GTA) was investigated for antibody immobilization. The monoclonal anti-cytokeratin-FITC (MACF) antibody was immobilized on the modified SiN surfaces. The modified surfaces were characterized by water contact angle measurements, atomic force microscopy and fluorescence microscopy. The FITC-fluorescent label indicated the existence of MACF antibody on the SiN surfaces and the efficiency of the silanization reaction. Absorption of APTES and GOPES on the oxidized SiN surfaces was computationally modeled and calculated by Materials Studio software. The computational and experimental results showed that modification of the SiN surfaces with APTES and GTA was more effective than the modification with GOPES. |
| doi_str_mv | 10.1088/2043-6262/6/4/045006 |
| format | Article |
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However few reports performed the experimental and computational studies together. In this study, surface modification of SiN surfaces with GOPES and APTES covalently bound with glutaraldehyde (GTA) was investigated for antibody immobilization. The monoclonal anti-cytokeratin-FITC (MACF) antibody was immobilized on the modified SiN surfaces. The modified surfaces were characterized by water contact angle measurements, atomic force microscopy and fluorescence microscopy. The FITC-fluorescent label indicated the existence of MACF antibody on the SiN surfaces and the efficiency of the silanization reaction. Absorption of APTES and GOPES on the oxidized SiN surfaces was computationally modeled and calculated by Materials Studio software. The computational and experimental results showed that modification of the SiN surfaces with APTES and GTA was more effective than the modification with GOPES.</description><identifier>ISSN: 2043-6262</identifier><identifier>ISSN: 2043-6254</identifier><identifier>EISSN: 2043-6254</identifier><identifier>EISSN: 2043-6262</identifier><identifier>DOI: 10.1088/2043-6262/6/4/045006</identifier><identifier>CODEN: ANSNCK</identifier><language>eng</language><publisher>IOP Publishing</publisher><subject>Antibodies ; APTES ; Computation ; Computer programs ; Contact angle ; GOPES ; Immobilization ; Nanostructure ; Silicon nitride ; Surface chemistry ; surface modification</subject><ispartof>Advances in natural sciences. 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Nanoscience and nanotechnology</title><addtitle>ANSN</addtitle><addtitle>Adv. Nat. Sci.: Nanosci. Nanotechnol</addtitle><description>Chemical modification of silicon nitride (SiN) surfaces by silanization has been widely studied especially with 3-(aminopropyl)triethoxysilane (APTES) and 3-(glycidyloxypropyl) dimethylethoxysilane (GOPES). However few reports performed the experimental and computational studies together. In this study, surface modification of SiN surfaces with GOPES and APTES covalently bound with glutaraldehyde (GTA) was investigated for antibody immobilization. The monoclonal anti-cytokeratin-FITC (MACF) antibody was immobilized on the modified SiN surfaces. The modified surfaces were characterized by water contact angle measurements, atomic force microscopy and fluorescence microscopy. The FITC-fluorescent label indicated the existence of MACF antibody on the SiN surfaces and the efficiency of the silanization reaction. Absorption of APTES and GOPES on the oxidized SiN surfaces was computationally modeled and calculated by Materials Studio software. The computational and experimental results showed that modification of the SiN surfaces with APTES and GTA was more effective than the modification with GOPES.</description><subject>Antibodies</subject><subject>APTES</subject><subject>Computation</subject><subject>Computer programs</subject><subject>Contact angle</subject><subject>GOPES</subject><subject>Immobilization</subject><subject>Nanostructure</subject><subject>Silicon nitride</subject><subject>Surface chemistry</subject><subject>surface modification</subject><issn>2043-6262</issn><issn>2043-6254</issn><issn>2043-6254</issn><issn>2043-6262</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>O3W</sourceid><recordid>eNqFkE9LxDAQxYsouKx-Aw-56WVtkk7b1JvI-gcUBfUc0jTBLG1TkxRdb35zs1sRD4K55M3wfsPMS5Ijgk8JZiylGLJFQQuaFimkGHKMi51k9t3OYfdHF3Q_OfR-hePLWFVkeJZ83tnGaCNFMLZHViNvWiOj7E1wplHIj04LqTx6M-EFXd0_LB-R6Bt0_vAUlbYuVsHUtlkj03W2jvjHdtgZkrYbxrAtRLuF1PugnOlUH2LDh7Exyh8ke1q0Xh1-__Pk-XL5dHG9uL2_urk4v11IABwWKqMlUKYA07qqoGJUA2FKVphJUTWUUJqXOdOiBsVEQeocdFmVlOSCEJJV2Tw5meYOzr6OygfeGS9V24pe2dFzwjAGyABYtMJklc5675TmQ9xauDUnmG9C55tE-SZRXnDgU-gRwxNm7MBXdnTxbP8fcvwHInrf_3LxodHZFwREkNU</recordid><startdate>20151201</startdate><enddate>20151201</enddate><creator>To, Thien Dien</creator><creator>Nguyen, Anh Tuan</creator><creator>Phan, Khoa Nhat Thanh</creator><creator>Truong, An Thu Thi</creator><creator>Doan, Tin Chanh Duc</creator><creator>Dang, Chien Mau</creator><general>IOP Publishing</general><scope>O3W</scope><scope>TSCCA</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U5</scope><scope>8FD</scope><scope>L7M</scope></search><sort><creationdate>20151201</creationdate><title>Modification of silicon nitride surfaces with GOPES and APTES for antibody immobilization: computational and experimental studies</title><author>To, Thien Dien ; Nguyen, Anh Tuan ; Phan, Khoa Nhat Thanh ; Truong, An Thu Thi ; Doan, Tin Chanh Duc ; Dang, Chien Mau</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c440t-e327428e402b994982f418ec908ca9d21225758fab4e8a61b54f797215a111393</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Antibodies</topic><topic>APTES</topic><topic>Computation</topic><topic>Computer programs</topic><topic>Contact angle</topic><topic>GOPES</topic><topic>Immobilization</topic><topic>Nanostructure</topic><topic>Silicon nitride</topic><topic>Surface chemistry</topic><topic>surface modification</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>To, Thien Dien</creatorcontrib><creatorcontrib>Nguyen, Anh Tuan</creatorcontrib><creatorcontrib>Phan, Khoa Nhat Thanh</creatorcontrib><creatorcontrib>Truong, An Thu Thi</creatorcontrib><creatorcontrib>Doan, Tin Chanh Duc</creatorcontrib><creatorcontrib>Dang, Chien Mau</creatorcontrib><collection>Institute of Physics Open Access Journal Titles</collection><collection>IOPscience (Open Access)</collection><collection>CrossRef</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Technology Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><jtitle>Advances in natural sciences. Nanoscience and nanotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>To, Thien Dien</au><au>Nguyen, Anh Tuan</au><au>Phan, Khoa Nhat Thanh</au><au>Truong, An Thu Thi</au><au>Doan, Tin Chanh Duc</au><au>Dang, Chien Mau</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Modification of silicon nitride surfaces with GOPES and APTES for antibody immobilization: computational and experimental studies</atitle><jtitle>Advances in natural sciences. Nanoscience and nanotechnology</jtitle><stitle>ANSN</stitle><addtitle>Adv. Nat. Sci.: Nanosci. Nanotechnol</addtitle><date>2015-12-01</date><risdate>2015</risdate><volume>6</volume><issue>4</issue><spage>45006</spage><epage>8</epage><pages>45006-8</pages><issn>2043-6262</issn><issn>2043-6254</issn><eissn>2043-6254</eissn><eissn>2043-6262</eissn><coden>ANSNCK</coden><abstract>Chemical modification of silicon nitride (SiN) surfaces by silanization has been widely studied especially with 3-(aminopropyl)triethoxysilane (APTES) and 3-(glycidyloxypropyl) dimethylethoxysilane (GOPES). However few reports performed the experimental and computational studies together. In this study, surface modification of SiN surfaces with GOPES and APTES covalently bound with glutaraldehyde (GTA) was investigated for antibody immobilization. The monoclonal anti-cytokeratin-FITC (MACF) antibody was immobilized on the modified SiN surfaces. The modified surfaces were characterized by water contact angle measurements, atomic force microscopy and fluorescence microscopy. The FITC-fluorescent label indicated the existence of MACF antibody on the SiN surfaces and the efficiency of the silanization reaction. Absorption of APTES and GOPES on the oxidized SiN surfaces was computationally modeled and calculated by Materials Studio software. The computational and experimental results showed that modification of the SiN surfaces with APTES and GTA was more effective than the modification with GOPES.</abstract><pub>IOP Publishing</pub><doi>10.1088/2043-6262/6/4/045006</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| source | IOP Publishing Journals; Institute of Physics Open Access Journal Titles; EZB-FREE-00999 freely available EZB journals; IOPscience extra; Institute of Physics (IOP) Journals - HEAL-Link; Free Full-Text Journals in Chemistry |
| subjects | Antibodies APTES Computation Computer programs Contact angle GOPES Immobilization Nanostructure Silicon nitride Surface chemistry surface modification |
| title | Modification of silicon nitride surfaces with GOPES and APTES for antibody immobilization: computational and experimental studies |
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