Subcritical water extraction of steviol glycosides from Stevia rebaudiana leaves and characterization of the raffinate phase
•Stevia rebaudiana leaves were subjected to subcritical water.•Yielding 38.67mg stevioside and 35.68mg rebaudioside A per g dry leaves.•Raffinate phase was analyzed for total chlorophyll, carotenoid and dietary fibers.•Yielding 31.91mg/100g, 5.71mg/100g and 4.98%, respectively.•S. rebaudiana leaves...
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| Veröffentlicht in: | The Journal of supercritical fluids 2014-11, Vol.95, p.422-430 |
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| creator | Yildiz-Ozturk, Ece Tag, Ozgur Yesil-Celiktas, Ozlem |
| description | •Stevia rebaudiana leaves were subjected to subcritical water.•Yielding 38.67mg stevioside and 35.68mg rebaudioside A per g dry leaves.•Raffinate phase was analyzed for total chlorophyll, carotenoid and dietary fibers.•Yielding 31.91mg/100g, 5.71mg/100g and 4.98%, respectively.•S. rebaudiana leaves can be utilized as sources of natural sweeteners, fibers and coloring agents in the industry.
The objectives of this work were to obtain steviol glycosides of S. rebaudiana leaves, possessing natural and noncaloric sweetener properties, using subcritical water extraction; to assess optimum extraction conditions; to determine biological activities of Stevia extracts and to characterize the raffinate phase. A Box–“Bhenken” statistical design was used to evaluate the effects of various values of temperature (100–150°C), time (30–60min) and flow rate (2–6ml/min) at a pressure of 230bar applying a solid/liquid ratio of 1:10 (m:v). The most effective parameter was temperature (p |
| doi_str_mv | 10.1016/j.supflu.2014.10.017 |
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The objectives of this work were to obtain steviol glycosides of S. rebaudiana leaves, possessing natural and noncaloric sweetener properties, using subcritical water extraction; to assess optimum extraction conditions; to determine biological activities of Stevia extracts and to characterize the raffinate phase. A Box–“Bhenken” statistical design was used to evaluate the effects of various values of temperature (100–150°C), time (30–60min) and flow rate (2–6ml/min) at a pressure of 230bar applying a solid/liquid ratio of 1:10 (m:v). The most effective parameter was temperature (p<0.005). Optimum extraction conditions were elicited as 125°C, 45min, 4ml/min flow rate which yielded 38.67mg/g stevioside and 35.68mg/g rebaudioside A. The total phenolic, flavonoid contents and DPPH free radical scavenging activity were found as 48.63mg gallic acid/g extract, 29.81mg quercetin/g extract and 92.50%, respectively. After extraction, total chlorophyll, carotenoid contents and dietary fibers were quantified as 31.91mg/100g, 5.71mg/100g and 4.98% in the raffinate phase. Hence, both extract and raffinate phases of S. rebaudiana leaves can be utilized as sources of natural sweeteners, fibers and coloring agents in the industry.</description><identifier>ISSN: 0896-8446</identifier><identifier>EISSN: 1872-8162</identifier><identifier>DOI: 10.1016/j.supflu.2014.10.017</identifier><language>eng</language><publisher>Elsevier B.V</publisher><subject>Dietary fiber ; Extraction ; Fibers ; Flavonoid ; Flow rate ; Glycosides ; Liquids ; Optimization ; Rebaudioside A ; Scavenging ; Stevia rebaudiana ; Stevioside ; Subcritical water extraction ; Sweeteners</subject><ispartof>The Journal of supercritical fluids, 2014-11, Vol.95, p.422-430</ispartof><rights>2014 Elsevier B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c339t-913e9fb3b7dd7c712066de0f1d05e76842c53caf31c5ff7c5cf9dcc04da909163</citedby><cites>FETCH-LOGICAL-c339t-913e9fb3b7dd7c712066de0f1d05e76842c53caf31c5ff7c5cf9dcc04da909163</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0896844614003532$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids></links><search><creatorcontrib>Yildiz-Ozturk, Ece</creatorcontrib><creatorcontrib>Tag, Ozgur</creatorcontrib><creatorcontrib>Yesil-Celiktas, Ozlem</creatorcontrib><title>Subcritical water extraction of steviol glycosides from Stevia rebaudiana leaves and characterization of the raffinate phase</title><title>The Journal of supercritical fluids</title><description>•Stevia rebaudiana leaves were subjected to subcritical water.•Yielding 38.67mg stevioside and 35.68mg rebaudioside A per g dry leaves.•Raffinate phase was analyzed for total chlorophyll, carotenoid and dietary fibers.•Yielding 31.91mg/100g, 5.71mg/100g and 4.98%, respectively.•S. rebaudiana leaves can be utilized as sources of natural sweeteners, fibers and coloring agents in the industry.
The objectives of this work were to obtain steviol glycosides of S. rebaudiana leaves, possessing natural and noncaloric sweetener properties, using subcritical water extraction; to assess optimum extraction conditions; to determine biological activities of Stevia extracts and to characterize the raffinate phase. A Box–“Bhenken” statistical design was used to evaluate the effects of various values of temperature (100–150°C), time (30–60min) and flow rate (2–6ml/min) at a pressure of 230bar applying a solid/liquid ratio of 1:10 (m:v). The most effective parameter was temperature (p<0.005). Optimum extraction conditions were elicited as 125°C, 45min, 4ml/min flow rate which yielded 38.67mg/g stevioside and 35.68mg/g rebaudioside A. The total phenolic, flavonoid contents and DPPH free radical scavenging activity were found as 48.63mg gallic acid/g extract, 29.81mg quercetin/g extract and 92.50%, respectively. After extraction, total chlorophyll, carotenoid contents and dietary fibers were quantified as 31.91mg/100g, 5.71mg/100g and 4.98% in the raffinate phase. Hence, both extract and raffinate phases of S. rebaudiana leaves can be utilized as sources of natural sweeteners, fibers and coloring agents in the industry.</description><subject>Dietary fiber</subject><subject>Extraction</subject><subject>Fibers</subject><subject>Flavonoid</subject><subject>Flow rate</subject><subject>Glycosides</subject><subject>Liquids</subject><subject>Optimization</subject><subject>Rebaudioside A</subject><subject>Scavenging</subject><subject>Stevia rebaudiana</subject><subject>Stevioside</subject><subject>Subcritical water extraction</subject><subject>Sweeteners</subject><issn>0896-8446</issn><issn>1872-8162</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><recordid>eNp9kM1r3DAQxUVpoduk_0EOOvbi7cgfsn0phJB-QCCHJGcxOxpltXitrSRvm5A_vjabXnMaePPeG-YnxIWCtQKlv-7WaTq4YVqXoOpZWoNq34mV6tqy6JQu34sVdL0uurrWH8WnlHYA0ICqVuLlbtpQ9NkTDvIPZo6S_-aIlH0YZXAyZT76MMjH4YlC8paTdDHs5d2io4y8wcl6HFEOjMd5i6OVtMWlgqN_xv9FecsyonN-nK_IwxYTn4sPDofEn1_nmXj4fn1_9bO4uf3x6-rypqCq6nPRq4p7t6k2rbUttaoErS2DUxYabnVXl9RUhK5S1DjXUkOut0RQW-yhV7o6E19OvYcYfk-cstn7RDwMOHKYklEdQF2Xpe5ma32yUgwpRXbmEP0e45NRYBbYZmdOsM0Ce1Fn2HPs2ynG8xtHz9Ek8jwSWx-ZsrHBv13wD-ABjfg</recordid><startdate>20141101</startdate><enddate>20141101</enddate><creator>Yildiz-Ozturk, Ece</creator><creator>Tag, Ozgur</creator><creator>Yesil-Celiktas, Ozlem</creator><general>Elsevier B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7U5</scope><scope>8FD</scope><scope>L7M</scope></search><sort><creationdate>20141101</creationdate><title>Subcritical water extraction of steviol glycosides from Stevia rebaudiana leaves and characterization of the raffinate phase</title><author>Yildiz-Ozturk, Ece ; Tag, Ozgur ; Yesil-Celiktas, Ozlem</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c339t-913e9fb3b7dd7c712066de0f1d05e76842c53caf31c5ff7c5cf9dcc04da909163</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Dietary fiber</topic><topic>Extraction</topic><topic>Fibers</topic><topic>Flavonoid</topic><topic>Flow rate</topic><topic>Glycosides</topic><topic>Liquids</topic><topic>Optimization</topic><topic>Rebaudioside A</topic><topic>Scavenging</topic><topic>Stevia rebaudiana</topic><topic>Stevioside</topic><topic>Subcritical water extraction</topic><topic>Sweeteners</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yildiz-Ozturk, Ece</creatorcontrib><creatorcontrib>Tag, Ozgur</creatorcontrib><creatorcontrib>Yesil-Celiktas, Ozlem</creatorcontrib><collection>CrossRef</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Technology Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><jtitle>The Journal of supercritical fluids</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yildiz-Ozturk, Ece</au><au>Tag, Ozgur</au><au>Yesil-Celiktas, Ozlem</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Subcritical water extraction of steviol glycosides from Stevia rebaudiana leaves and characterization of the raffinate phase</atitle><jtitle>The Journal of supercritical fluids</jtitle><date>2014-11-01</date><risdate>2014</risdate><volume>95</volume><spage>422</spage><epage>430</epage><pages>422-430</pages><issn>0896-8446</issn><eissn>1872-8162</eissn><abstract>•Stevia rebaudiana leaves were subjected to subcritical water.•Yielding 38.67mg stevioside and 35.68mg rebaudioside A per g dry leaves.•Raffinate phase was analyzed for total chlorophyll, carotenoid and dietary fibers.•Yielding 31.91mg/100g, 5.71mg/100g and 4.98%, respectively.•S. rebaudiana leaves can be utilized as sources of natural sweeteners, fibers and coloring agents in the industry.
The objectives of this work were to obtain steviol glycosides of S. rebaudiana leaves, possessing natural and noncaloric sweetener properties, using subcritical water extraction; to assess optimum extraction conditions; to determine biological activities of Stevia extracts and to characterize the raffinate phase. A Box–“Bhenken” statistical design was used to evaluate the effects of various values of temperature (100–150°C), time (30–60min) and flow rate (2–6ml/min) at a pressure of 230bar applying a solid/liquid ratio of 1:10 (m:v). The most effective parameter was temperature (p<0.005). Optimum extraction conditions were elicited as 125°C, 45min, 4ml/min flow rate which yielded 38.67mg/g stevioside and 35.68mg/g rebaudioside A. The total phenolic, flavonoid contents and DPPH free radical scavenging activity were found as 48.63mg gallic acid/g extract, 29.81mg quercetin/g extract and 92.50%, respectively. After extraction, total chlorophyll, carotenoid contents and dietary fibers were quantified as 31.91mg/100g, 5.71mg/100g and 4.98% in the raffinate phase. Hence, both extract and raffinate phases of S. rebaudiana leaves can be utilized as sources of natural sweeteners, fibers and coloring agents in the industry.</abstract><pub>Elsevier B.V</pub><doi>10.1016/j.supflu.2014.10.017</doi><tpages>9</tpages></addata></record> |
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| subjects | Dietary fiber Extraction Fibers Flavonoid Flow rate Glycosides Liquids Optimization Rebaudioside A Scavenging Stevia rebaudiana Stevioside Subcritical water extraction Sweeteners |
| title | Subcritical water extraction of steviol glycosides from Stevia rebaudiana leaves and characterization of the raffinate phase |
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