Ligation-Independent Mechanism of Multiplex Ligation-Dependent Probe Amplification
Multiplex ligation-dependent probe amplification (MLPA) is a widely used technique for detecting genomic structural variants. The technique is based on hybridization and ligation, followed by amplification of the ligation products. Therefore, ligation is considered a fundamental process that determi...
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| Veröffentlicht in: | Analytical Sciences 2014/08/10, Vol.30(8), pp.805-810 |
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| creator | UNO, Naoki YANAGIHARA, Katsunori |
| description | Multiplex ligation-dependent probe amplification (MLPA) is a widely used technique for detecting genomic structural variants. The technique is based on hybridization and ligation, followed by amplification of the ligation products. Therefore, ligation is considered a fundamental process that determines the feasibility and fidelity of MLPA. However, despite the widespread use of this technique, its reaction mechanism has not been fully analyzed. Herein, we describe a ligation-independent pathway for MLPA and introduce a ligation-independent probe amplification system that can be used to obtain amplified products without the hybridization and ligation processes. Fragment analysis revealed that the ligation-independent pathway is functional and that the capacity to discriminate single nucleotides with MLPA does not depend on ligation. These findings indicate that the feasibility and fidelity of MLPA do not rely on ligation. |
| doi_str_mv | 10.2116/analsci.30.805 |
| format | Article |
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| source | J-STAGE Free; MEDLINE; Freely Accessible Japanese Titles; EZB-FREE-00999 freely available EZB journals; Free Full-Text Journals in Chemistry; SpringerLink Journals - AutoHoldings |
| subjects | Amplification Analytical Chemistry Chemistry Feasibility Feasibility Studies fragment analysis Fragmentation helicase-dependent amplification ligation Mathematical analysis MLPA Molecular Probes Multiplexing Nucleotides Original Papers Pathways Reaction mechanisms |
| title | Ligation-Independent Mechanism of Multiplex Ligation-Dependent Probe Amplification |
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