A study of bioactive, branched (1→3)-β-d-glucans in dimethylacetamide/LiCl and dimethyl sulphoxide/LiCl using size-exclusion chromatography with multi-angle light scattering detection

•DMAc/LiCl and DMSO/LiCl were evaluated and compared as solvents for SEC-MALLS of β-(1→3)-d-glucans.•A new SEC-MALLS protocol based on DMAc/LiCl as solvent was developed.•A kinetic model for depolymerization upon heat treatment in DMAc/LiCl is provided.•SEC-MALLS in DMSO prone to artefacts and low s...

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Veröffentlicht in:	Journal of Chromatography A 2013-08, Vol.1305, p.109-113
Hauptverfasser:	Qin, Fen, Kes, Mürşide, Christensen, Bjørn E.
Format:	Artikel
Sprache:	eng
Schlagworte:	(1 → 3)-β-d-Glucan Acetamides - chemistry Analytical, structural and metabolic biochemistry Applied sciences bioactive properties Biological and medical sciences Carbohydrates cell walls Chromatography Chromatography, Gel - methods depolymerization derivatization Detectors Dimethyl sulfoxide Dimethyl Sulfoxide - chemistry Dimethyl sulphoxide (DMSO) Dimethylacetamide (DMAc) Dissolution Exact sciences and technology Fundamental and applied biological sciences. Psychology gel chromatography Glucan glucans Glucans - analysis heat Heating Light Light scattering lithium chloride Lithium Chloride - chemistry Miscellaneous molecular weight Organic polymers Other biological molecules Physicochemistry of polymers Properties and characterization Refractive index increment Saccharomyces cerevisiae Scattering, Radiation SEC-MALLS Solubility Solvents Structure, morphology and analysis temperature
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