Hydrophilic interaction chromatography for selective separation of isomeric saponins

•An efficient HILIC method was developed to separate isomeric saponins.•A Click XIon column with improved hydrophilicity and polar selectivity was used.•Methanol was the weak eluent, and symmetrical peak in high sample loading was obtained.•Eleven saponins were isolated and identified.•A new saponin...

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