Genomic targeting and high-resolution mapping of the Tsn1 gene in wheat
Tan spot, caused by the fungal pathogen Pyrenophora tritici-repentis (Died.) Drechs. causes severe yield losses in wheat (Triticum aestivum L., 2n = 6x = 42, AABBDD) and durum (T. turgidum L., 2n = 4x = 28, AABB). The Tsn1 gene acts dominantly to confer sensitivity to a host-selective proteinaceous...
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| description | Tan spot, caused by the fungal pathogen Pyrenophora tritici-repentis (Died.) Drechs. causes severe yield losses in wheat (Triticum aestivum L., 2n = 6x = 42, AABBDD) and durum (T. turgidum L., 2n = 4x = 28, AABB). The Tsn1 gene acts dominantly to confer sensitivity to a host-selective proteinaceous toxin (Ptr ToxA) produced by the fungus. Our objectives were to: (i) target markers to the Tsn1 genomic region and (ii) develop a high-resolution map of the Tsn1 locus. The techniques of methylation-sensitive AFLP, traditional AFLP, and cDNA-AFLP were combined with bulked segregant analysis (BSA) using various wheat and durum cytogenetic stocks to target markers to the Tsn1 genomic region. Over 500 primer combinations were screened resulting in the identification of 18 low-copy markers closely linked to Tsn1. High-resolution mapping of the markers delineated the Tsn1 gene to a 0.2 cM interval in a hexaploid wheat population consisting of 1266 gametes, and to 0.8 cM in a durum wheat population consisting of 1860 gametes. Comparisons with rice BAC/PAC sequences indicated the lack of colinearity within the Tsn1 genomic region. Tsn1 was located within a gene-rich recombination hot spot region, and the physical distance separating the flanking markers may be as little as 200 kb. Therefore, these markers will serve as a basis for the map-based cloning of Tsn1. The isolation of Tsn1 will further our knowledge of wheat-tan spot interactions as well as host-pathogen interactions in general. |
| doi_str_mv | 10.2135/cropsci2004.9510 |
| format | Article |
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Drechs. causes severe yield losses in wheat (Triticum aestivum L., 2n = 6x = 42, AABBDD) and durum (T. turgidum L., 2n = 4x = 28, AABB). The Tsn1 gene acts dominantly to confer sensitivity to a host-selective proteinaceous toxin (Ptr ToxA) produced by the fungus. Our objectives were to: (i) target markers to the Tsn1 genomic region and (ii) develop a high-resolution map of the Tsn1 locus. The techniques of methylation-sensitive AFLP, traditional AFLP, and cDNA-AFLP were combined with bulked segregant analysis (BSA) using various wheat and durum cytogenetic stocks to target markers to the Tsn1 genomic region. Over 500 primer combinations were screened resulting in the identification of 18 low-copy markers closely linked to Tsn1. High-resolution mapping of the markers delineated the Tsn1 gene to a 0.2 cM interval in a hexaploid wheat population consisting of 1266 gametes, and to 0.8 cM in a durum wheat population consisting of 1860 gametes. Comparisons with rice BAC/PAC sequences indicated the lack of colinearity within the Tsn1 genomic region. Tsn1 was located within a gene-rich recombination hot spot region, and the physical distance separating the flanking markers may be as little as 200 kb. Therefore, these markers will serve as a basis for the map-based cloning of Tsn1. The isolation of Tsn1 will further our knowledge of wheat-tan spot interactions as well as host-pathogen interactions in general.</description><identifier>ISSN: 0011-183X</identifier><identifier>EISSN: 1435-0653</identifier><identifier>DOI: 10.2135/cropsci2004.9510</identifier><identifier>CODEN: CRPSAY</identifier><language>eng</language><publisher>Madison: Crop Science Society of America</publisher><subject>Agronomy. Soil science and plant productions ; amplified fragment length polymorphism ; Biological and medical sciences ; chromosome mapping ; Cloning ; complementary DNA ; Crop diseases ; Cytogenetics ; durum wheat ; Fundamental and applied biological sciences. Psychology ; fungal diseases of plants ; Generalities. Genetics. Plant material ; genes ; genetic markers ; genetic recombination ; Genetics ; Genetics and breeding of economic plants ; Genomics ; grain crops ; host plants ; leaf spotting ; loci ; mycotoxins ; Pathogens ; plant pathogenic fungi ; Pyrenophora tritici-repentis ; Toxins ; Triticum aestivum ; Triticum turgidum subsp. durum ; Wheat</subject><ispartof>Crop science, 2004-05, Vol.44 (3), p.951-962</ispartof><rights>Crop Science Society of America</rights><rights>2004 INIST-CNRS</rights><rights>COPYRIGHT 2004 Crop Science Society of America</rights><rights>Copyright American Society of Agronomy May/Jun 2004</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4030-902e72911079004e0697b5b851ba77abaca63668a345f9830368f5298a19e8a93</citedby><cites>FETCH-LOGICAL-c4030-902e72911079004e0697b5b851ba77abaca63668a345f9830368f5298a19e8a93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.2135%2Fcropsci2004.9510$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.2135%2Fcropsci2004.9510$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27903,27904,45553,45554</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15764362$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Haen, K.M</creatorcontrib><creatorcontrib>Lu, H</creatorcontrib><creatorcontrib>Friesen, T.L</creatorcontrib><creatorcontrib>Faris, J.D</creatorcontrib><title>Genomic targeting and high-resolution mapping of the Tsn1 gene in wheat</title><title>Crop science</title><description>Tan spot, caused by the fungal pathogen Pyrenophora tritici-repentis (Died.) Drechs. causes severe yield losses in wheat (Triticum aestivum L., 2n = 6x = 42, AABBDD) and durum (T. turgidum L., 2n = 4x = 28, AABB). The Tsn1 gene acts dominantly to confer sensitivity to a host-selective proteinaceous toxin (Ptr ToxA) produced by the fungus. Our objectives were to: (i) target markers to the Tsn1 genomic region and (ii) develop a high-resolution map of the Tsn1 locus. The techniques of methylation-sensitive AFLP, traditional AFLP, and cDNA-AFLP were combined with bulked segregant analysis (BSA) using various wheat and durum cytogenetic stocks to target markers to the Tsn1 genomic region. Over 500 primer combinations were screened resulting in the identification of 18 low-copy markers closely linked to Tsn1. High-resolution mapping of the markers delineated the Tsn1 gene to a 0.2 cM interval in a hexaploid wheat population consisting of 1266 gametes, and to 0.8 cM in a durum wheat population consisting of 1860 gametes. Comparisons with rice BAC/PAC sequences indicated the lack of colinearity within the Tsn1 genomic region. Tsn1 was located within a gene-rich recombination hot spot region, and the physical distance separating the flanking markers may be as little as 200 kb. Therefore, these markers will serve as a basis for the map-based cloning of Tsn1. The isolation of Tsn1 will further our knowledge of wheat-tan spot interactions as well as host-pathogen interactions in general.</description><subject>Agronomy. Soil science and plant productions</subject><subject>amplified fragment length polymorphism</subject><subject>Biological and medical sciences</subject><subject>chromosome mapping</subject><subject>Cloning</subject><subject>complementary DNA</subject><subject>Crop diseases</subject><subject>Cytogenetics</subject><subject>durum wheat</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>fungal diseases of plants</subject><subject>Generalities. Genetics. Plant material</subject><subject>genes</subject><subject>genetic markers</subject><subject>genetic recombination</subject><subject>Genetics</subject><subject>Genetics and breeding of economic plants</subject><subject>Genomics</subject><subject>grain crops</subject><subject>host plants</subject><subject>leaf spotting</subject><subject>loci</subject><subject>mycotoxins</subject><subject>Pathogens</subject><subject>plant pathogenic fungi</subject><subject>Pyrenophora tritici-repentis</subject><subject>Toxins</subject><subject>Triticum aestivum</subject><subject>Triticum turgidum subsp. durum</subject><subject>Wheat</subject><issn>0011-183X</issn><issn>1435-0653</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqFUd2L1DAQL6Lgevrum0XQt66TpPl6XMq5HhycuHfgW5jtpd0c3aQmXY77703pgiIHkkBg8vuYmV9RvCewpoTxL20MY2odBajXmhN4UaxIzXgFgrOXxQqAkIoo9vN18SalBwCQWvJVsd1aH46uLSeMvZ2c70v09-XB9Ycq2hSG0-SCL484jvNf6MrpYMvb5EnZW29L58vHg8XpbfGqwyHZd-f3orj7ennbfKuub7ZXzea6amtgUGmgVlJNSLbPnVoQWu75XnGyRylxjy0KJoRCVvNOKwZMqI5TrZBoq1Czi-LzojvG8Otk02SOLrV2GNDbcEqGSK0krWUGfvwH-BBO0efeDCVU1EIqnkHVAupxsMb5LkwR23mwiEPwtnO5vCFE5ssUzfj1M_h87m3e4bMEWAg5npSi7cwY3RHjkyFg5tzMX7mZObdM-XRuHFOLQxfRty794XEpaiZm6c2Ce8yeT__VNc2uoc2Pm--75mounr0-LBodBoN9zD53OwqEAYG8-pqz3-yHszQ</recordid><startdate>200405</startdate><enddate>200405</enddate><creator>Haen, K.M</creator><creator>Lu, H</creator><creator>Friesen, T.L</creator><creator>Faris, J.D</creator><general>Crop Science Society of America</general><general>American Society of Agronomy</general><scope>FBQ</scope><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X2</scope><scope>7XB</scope><scope>88I</scope><scope>8AF</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FK</scope><scope>8G5</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>HCIFZ</scope><scope>L6V</scope><scope>M0K</scope><scope>M2O</scope><scope>M2P</scope><scope>M7S</scope><scope>MBDVC</scope><scope>PATMY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>Q9U</scope><scope>R05</scope><scope>S0X</scope><scope>8FD</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>200405</creationdate><title>Genomic targeting and high-resolution mapping of the Tsn1 gene in wheat</title><author>Haen, K.M ; Lu, H ; Friesen, T.L ; Faris, J.D</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4030-902e72911079004e0697b5b851ba77abaca63668a345f9830368f5298a19e8a93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Agronomy. Soil science and plant productions</topic><topic>amplified fragment length polymorphism</topic><topic>Biological and medical sciences</topic><topic>chromosome mapping</topic><topic>Cloning</topic><topic>complementary DNA</topic><topic>Crop diseases</topic><topic>Cytogenetics</topic><topic>durum wheat</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>fungal diseases of plants</topic><topic>Generalities. Genetics. Plant material</topic><topic>genes</topic><topic>genetic markers</topic><topic>genetic recombination</topic><topic>Genetics</topic><topic>Genetics and breeding of economic plants</topic><topic>Genomics</topic><topic>grain crops</topic><topic>host plants</topic><topic>leaf spotting</topic><topic>loci</topic><topic>mycotoxins</topic><topic>Pathogens</topic><topic>plant pathogenic fungi</topic><topic>Pyrenophora tritici-repentis</topic><topic>Toxins</topic><topic>Triticum aestivum</topic><topic>Triticum turgidum subsp. durum</topic><topic>Wheat</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Haen, K.M</creatorcontrib><creatorcontrib>Lu, H</creatorcontrib><creatorcontrib>Friesen, T.L</creatorcontrib><creatorcontrib>Faris, J.D</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Agricultural Science Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Science Database (Alumni Edition)</collection><collection>STEM Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Engineering Collection</collection><collection>Agricultural Science Database</collection><collection>Research Library</collection><collection>Science Database</collection><collection>Engineering Database</collection><collection>Research Library (Corporate)</collection><collection>Environmental Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>ProQuest Central Basic</collection><collection>University of Michigan</collection><collection>SIRS Editorial</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Crop science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Haen, K.M</au><au>Lu, H</au><au>Friesen, T.L</au><au>Faris, J.D</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genomic targeting and high-resolution mapping of the Tsn1 gene in wheat</atitle><jtitle>Crop science</jtitle><date>2004-05</date><risdate>2004</risdate><volume>44</volume><issue>3</issue><spage>951</spage><epage>962</epage><pages>951-962</pages><issn>0011-183X</issn><eissn>1435-0653</eissn><coden>CRPSAY</coden><abstract>Tan spot, caused by the fungal pathogen Pyrenophora tritici-repentis (Died.) Drechs. causes severe yield losses in wheat (Triticum aestivum L., 2n = 6x = 42, AABBDD) and durum (T. turgidum L., 2n = 4x = 28, AABB). The Tsn1 gene acts dominantly to confer sensitivity to a host-selective proteinaceous toxin (Ptr ToxA) produced by the fungus. Our objectives were to: (i) target markers to the Tsn1 genomic region and (ii) develop a high-resolution map of the Tsn1 locus. The techniques of methylation-sensitive AFLP, traditional AFLP, and cDNA-AFLP were combined with bulked segregant analysis (BSA) using various wheat and durum cytogenetic stocks to target markers to the Tsn1 genomic region. Over 500 primer combinations were screened resulting in the identification of 18 low-copy markers closely linked to Tsn1. High-resolution mapping of the markers delineated the Tsn1 gene to a 0.2 cM interval in a hexaploid wheat population consisting of 1266 gametes, and to 0.8 cM in a durum wheat population consisting of 1860 gametes. Comparisons with rice BAC/PAC sequences indicated the lack of colinearity within the Tsn1 genomic region. Tsn1 was located within a gene-rich recombination hot spot region, and the physical distance separating the flanking markers may be as little as 200 kb. Therefore, these markers will serve as a basis for the map-based cloning of Tsn1. The isolation of Tsn1 will further our knowledge of wheat-tan spot interactions as well as host-pathogen interactions in general.</abstract><cop>Madison</cop><pub>Crop Science Society of America</pub><doi>10.2135/cropsci2004.9510</doi><tpages>12</tpages></addata></record> |
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| subjects | Agronomy. Soil science and plant productions amplified fragment length polymorphism Biological and medical sciences chromosome mapping Cloning complementary DNA Crop diseases Cytogenetics durum wheat Fundamental and applied biological sciences. Psychology fungal diseases of plants Generalities. Genetics. Plant material genes genetic markers genetic recombination Genetics Genetics and breeding of economic plants Genomics grain crops host plants leaf spotting loci mycotoxins Pathogens plant pathogenic fungi Pyrenophora tritici-repentis Toxins Triticum aestivum Triticum turgidum subsp. durum Wheat |
| title | Genomic targeting and high-resolution mapping of the Tsn1 gene in wheat |
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