Involvement of phosphoglucose isomerase in pathogenicity of Xanthomonas oryzae pv. oryzae
ABSTRACT Xanthomonas oryzae pv. oryzae, the causal agent of bacterial leaf blight of rice, was subjected to transposon mutagenesis to generate mutants defective in pathogenicity. A novel mutant 74M913 was attenuated in virulence but retained its ability to cause the hypersensitive response in leaf b...
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| Veröffentlicht in: | Phytopathology 2004-05, Vol.94 (5), p.478-483 |
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| creator | TSUGE, Seiji OCHIAI, Hirokazu INOUE, Yasuhiro OKU, Takashi TSUNO, Kazunori KAKU, Hisatoshi KUBO, Yasuyuki |
| description | ABSTRACT Xanthomonas oryzae pv. oryzae, the causal agent of bacterial leaf blight of rice, was subjected to transposon mutagenesis to generate mutants defective in pathogenicity. A novel mutant 74M913 was attenuated in virulence but retained its ability to cause the hypersensitive response in leaf blight-resistant rice and tomato. Cloning and sequence analysis revealed that the transposon in 74M913 was inserted in a gene homologous to the phosphoglucose isomerase (pgi) gene of X. axonopodis pv. citri. Growth of the mutant in a synthetic medium containing fructose or xylose as a sole carbohydrate source was much reduced, indicating the transposon disrupted pgi function. The interaction between expression of pgi and hypersensitive response and pathogenicity (hrp) genes was investigated because we had demonstrated previously that expression of hrp genes of X. oryzae pv. oryzae is induced in a synthetic medium containing xylose. However, pgi and the hrp gene (hrcU) were expressed independently. This study suggests that PGI is involved in pathogenicity of X. oryzae pv. oryzae. |
| doi_str_mv | 10.1094/phyto.2004.94.5.478 |
| format | Article |
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A novel mutant 74M913 was attenuated in virulence but retained its ability to cause the hypersensitive response in leaf blight-resistant rice and tomato. Cloning and sequence analysis revealed that the transposon in 74M913 was inserted in a gene homologous to the phosphoglucose isomerase (pgi) gene of X. axonopodis pv. citri. Growth of the mutant in a synthetic medium containing fructose or xylose as a sole carbohydrate source was much reduced, indicating the transposon disrupted pgi function. The interaction between expression of pgi and hypersensitive response and pathogenicity (hrp) genes was investigated because we had demonstrated previously that expression of hrp genes of X. oryzae pv. oryzae is induced in a synthetic medium containing xylose. However, pgi and the hrp gene (hrcU) were expressed independently. This study suggests that PGI is involved in pathogenicity of X. oryzae pv. oryzae.</description><identifier>ISSN: 0031-949X</identifier><identifier>EISSN: 1943-7684</identifier><identifier>DOI: 10.1094/phyto.2004.94.5.478</identifier><identifier>PMID: 18943766</identifier><identifier>CODEN: PHYTAJ</identifier><language>eng</language><publisher>St. Paul, MN: American Phytopathological Society</publisher><subject>Bacterial plant pathogens ; Biological and medical sciences ; Fundamental and applied biological sciences. Psychology ; Phytopathology. Animal pests. 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| source | American Phytopathological Society; Alma/SFX Local Collection; EZB Electronic Journals Library |
| subjects | Bacterial plant pathogens Biological and medical sciences Fundamental and applied biological sciences. Psychology Phytopathology. Animal pests. Plant and forest protection Xanthomonas oryzae |
| title | Involvement of phosphoglucose isomerase in pathogenicity of Xanthomonas oryzae pv. oryzae |
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