Effect of 3-nitrooxypropanol on methane and hydrogen emissions, methane isotopic signature, and ruminal fermentation in dairy cows
The objective of this crossover experiment was to investigate the effect of a methane inhibitor, 3-nitrooxypropanol (3NOP), on enteric methane emission, methane isotopic composition, and rumen fermentation and microbial profile in lactating dairy cows. The experiment involved 6 ruminally cannulated...
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| Veröffentlicht in: | Journal of dairy science 2016-07, Vol.99 (7), p.5335-5344 |
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| creator | Lopes, J.C. de Matos, L.F. Harper, M.T. Giallongo, F. Oh, J. Gruen, D. Ono, S. Kindermann, M. Duval, S. Hristov, A.N. |
| description | The objective of this crossover experiment was to investigate the effect of a methane inhibitor, 3-nitrooxypropanol (3NOP), on enteric methane emission, methane isotopic composition, and rumen fermentation and microbial profile in lactating dairy cows. The experiment involved 6 ruminally cannulated late-lactation Holstein cows assigned to 2 treatments: control and 3NOP (60 mg/kg of feed dry matter). Compared with the control, 3NOP decreased methane emission by 31% and increased hydrogen emission from undetectable to 1.33 g/d. Methane emissions per kilogram of dry matter intake and milk yield were also decreased 34% by 3NOP. Milk production and composition were not affected by 3NOP, except milk fat concentration was increased compared with the control. Concentrations of total VFA and propionate in ruminal fluid were not affected by treatment, but acetate concentration tended to be lower and acetate-to-propionate ratio was lower for 3NOP compared with the control. The 3NOP decreased the molar proportion of acetate and increase those of propionate, butyrate, valerate, and isovalerate. Deuterium-to-hydrogen ratios of methane and the abundance of 13CH3D were similar between treatments. Compared with the control, minor (4‰) depletion in the 13C/12C ratio was observed for 3NOP. Genus composition of methanogenic archaea (Methanobrevibacter, Methanosphaera, and Methanomicrobium) was not affected by 3NOP, but the proportion of methanogens in the total cell counts tended to be decreased by 3NOP. Prevotella spp., the predominant bacterial genus in ruminal contents in this experiment, was also not affected by 3NOP. Compared with the control, Ruminococcus and Clostridium spp. were decreased and Butyrivibrio spp. was increased by 3NOP. This experiment demonstrated that a substantial inhibition of enteric methane emission by 3NOP in dairy cows was accompanied with increased hydrogen emission and decreased acetate-to-propionate ratio; however, neither an effect on rumen archaeal community composition nor a significant change in the isotope composition of methane was observed. |
| doi_str_mv | 10.3168/jds.2015-10832 |
| format | Article |
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The experiment involved 6 ruminally cannulated late-lactation Holstein cows assigned to 2 treatments: control and 3NOP (60 mg/kg of feed dry matter). Compared with the control, 3NOP decreased methane emission by 31% and increased hydrogen emission from undetectable to 1.33 g/d. Methane emissions per kilogram of dry matter intake and milk yield were also decreased 34% by 3NOP. Milk production and composition were not affected by 3NOP, except milk fat concentration was increased compared with the control. Concentrations of total VFA and propionate in ruminal fluid were not affected by treatment, but acetate concentration tended to be lower and acetate-to-propionate ratio was lower for 3NOP compared with the control. The 3NOP decreased the molar proportion of acetate and increase those of propionate, butyrate, valerate, and isovalerate. Deuterium-to-hydrogen ratios of methane and the abundance of 13CH3D were similar between treatments. Compared with the control, minor (4‰) depletion in the 13C/12C ratio was observed for 3NOP. Genus composition of methanogenic archaea (Methanobrevibacter, Methanosphaera, and Methanomicrobium) was not affected by 3NOP, but the proportion of methanogens in the total cell counts tended to be decreased by 3NOP. Prevotella spp., the predominant bacterial genus in ruminal contents in this experiment, was also not affected by 3NOP. Compared with the control, Ruminococcus and Clostridium spp. were decreased and Butyrivibrio spp. was increased by 3NOP. This experiment demonstrated that a substantial inhibition of enteric methane emission by 3NOP in dairy cows was accompanied with increased hydrogen emission and decreased acetate-to-propionate ratio; however, neither an effect on rumen archaeal community composition nor a significant change in the isotope composition of methane was observed.</description><identifier>ISSN: 0022-0302</identifier><identifier>EISSN: 1525-3198</identifier><identifier>DOI: 10.3168/jds.2015-10832</identifier><identifier>PMID: 27085412</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>3-nitrooxypropanol ; Animals ; Archaea - drug effects ; Archaea - physiology ; Carbon Isotopes - analysis ; Cattle - physiology ; Cross-Over Studies ; dairy cow ; Deuterium - analysis ; Female ; Fermentation - drug effects ; Gastrointestinal Contents - chemistry ; Gastrointestinal Contents - drug effects ; Lactation ; methane ; Methane - metabolism ; Milk - secretion ; Propanols - pharmacology ; Rumen - metabolism ; Rumen - microbiology ; rumen fermentation</subject><ispartof>Journal of dairy science, 2016-07, Vol.99 (7), p.5335-5344</ispartof><rights>2016 American Dairy Science Association</rights><rights>Copyright © 2016 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c450t-7db5d54956b8ca82b5a15a317f31e7a41f15cc7f7335b52229f2b1edfec10dfb3</citedby><cites>FETCH-LOGICAL-c450t-7db5d54956b8ca82b5a15a317f31e7a41f15cc7f7335b52229f2b1edfec10dfb3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0022030216301801$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27085412$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lopes, J.C.</creatorcontrib><creatorcontrib>de Matos, L.F.</creatorcontrib><creatorcontrib>Harper, M.T.</creatorcontrib><creatorcontrib>Giallongo, F.</creatorcontrib><creatorcontrib>Oh, J.</creatorcontrib><creatorcontrib>Gruen, D.</creatorcontrib><creatorcontrib>Ono, S.</creatorcontrib><creatorcontrib>Kindermann, M.</creatorcontrib><creatorcontrib>Duval, S.</creatorcontrib><creatorcontrib>Hristov, A.N.</creatorcontrib><title>Effect of 3-nitrooxypropanol on methane and hydrogen emissions, methane isotopic signature, and ruminal fermentation in dairy cows</title><title>Journal of dairy science</title><addtitle>J Dairy Sci</addtitle><description>The objective of this crossover experiment was to investigate the effect of a methane inhibitor, 3-nitrooxypropanol (3NOP), on enteric methane emission, methane isotopic composition, and rumen fermentation and microbial profile in lactating dairy cows. The experiment involved 6 ruminally cannulated late-lactation Holstein cows assigned to 2 treatments: control and 3NOP (60 mg/kg of feed dry matter). Compared with the control, 3NOP decreased methane emission by 31% and increased hydrogen emission from undetectable to 1.33 g/d. Methane emissions per kilogram of dry matter intake and milk yield were also decreased 34% by 3NOP. Milk production and composition were not affected by 3NOP, except milk fat concentration was increased compared with the control. Concentrations of total VFA and propionate in ruminal fluid were not affected by treatment, but acetate concentration tended to be lower and acetate-to-propionate ratio was lower for 3NOP compared with the control. The 3NOP decreased the molar proportion of acetate and increase those of propionate, butyrate, valerate, and isovalerate. Deuterium-to-hydrogen ratios of methane and the abundance of 13CH3D were similar between treatments. Compared with the control, minor (4‰) depletion in the 13C/12C ratio was observed for 3NOP. Genus composition of methanogenic archaea (Methanobrevibacter, Methanosphaera, and Methanomicrobium) was not affected by 3NOP, but the proportion of methanogens in the total cell counts tended to be decreased by 3NOP. Prevotella spp., the predominant bacterial genus in ruminal contents in this experiment, was also not affected by 3NOP. Compared with the control, Ruminococcus and Clostridium spp. were decreased and Butyrivibrio spp. was increased by 3NOP. This experiment demonstrated that a substantial inhibition of enteric methane emission by 3NOP in dairy cows was accompanied with increased hydrogen emission and decreased acetate-to-propionate ratio; however, neither an effect on rumen archaeal community composition nor a significant change in the isotope composition of methane was observed.</description><subject>3-nitrooxypropanol</subject><subject>Animals</subject><subject>Archaea - drug effects</subject><subject>Archaea - physiology</subject><subject>Carbon Isotopes - analysis</subject><subject>Cattle - physiology</subject><subject>Cross-Over Studies</subject><subject>dairy cow</subject><subject>Deuterium - analysis</subject><subject>Female</subject><subject>Fermentation - drug effects</subject><subject>Gastrointestinal Contents - chemistry</subject><subject>Gastrointestinal Contents - drug effects</subject><subject>Lactation</subject><subject>methane</subject><subject>Methane - metabolism</subject><subject>Milk - secretion</subject><subject>Propanols - pharmacology</subject><subject>Rumen - metabolism</subject><subject>Rumen - microbiology</subject><subject>rumen fermentation</subject><issn>0022-0302</issn><issn>1525-3198</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kEFvFCEYhonR2G316tFw9NBZ-WDYYY6mqdqkiRc9EwY-WpoZWIHR7tVfLu3W3jwRwvO-4X0IeQdsK2CnPt65suUMZAdMCf6CbEBy2QkY1UuyYYzzjgnGT8hpKXftCpzJ1-SED0zJHviG_Ln0Hm2lyVPRxVBzSveHfU57E9NMU6QL1lsTkZro6O3B5XSDkeISSgkplvPn91BSTftgaQk30dQ14_ljJq9LiGamHvOCsZraYjRE6kzIB2rT7_KGvPJmLvj26TwjPz5ffr_42l1_-3J18em6s71ktRvcJJ3sR7mblDWKT9KANAIGLwAH04MHae3gByHkJDnno-cToGvzgDk_iTPy4djb5v1csVTdVlic5_b9tBYNwzio3ah2fUO3R9TmVEpGr_c5LCYfNDD94F037_rBu3703gLvn7rXaUH3jP8T3QB1BLAt_BUw62IDRosu5OZfuxT-1_0Xi9eUGg</recordid><startdate>201607</startdate><enddate>201607</enddate><creator>Lopes, J.C.</creator><creator>de Matos, L.F.</creator><creator>Harper, M.T.</creator><creator>Giallongo, F.</creator><creator>Oh, J.</creator><creator>Gruen, D.</creator><creator>Ono, S.</creator><creator>Kindermann, M.</creator><creator>Duval, S.</creator><creator>Hristov, A.N.</creator><general>Elsevier Inc</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201607</creationdate><title>Effect of 3-nitrooxypropanol on methane and hydrogen emissions, methane isotopic signature, and ruminal fermentation in dairy cows</title><author>Lopes, J.C. ; de Matos, L.F. ; Harper, M.T. ; Giallongo, F. ; Oh, J. ; Gruen, D. ; Ono, S. ; Kindermann, M. ; Duval, S. ; Hristov, A.N.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c450t-7db5d54956b8ca82b5a15a317f31e7a41f15cc7f7335b52229f2b1edfec10dfb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>3-nitrooxypropanol</topic><topic>Animals</topic><topic>Archaea - drug effects</topic><topic>Archaea - physiology</topic><topic>Carbon Isotopes - analysis</topic><topic>Cattle - physiology</topic><topic>Cross-Over Studies</topic><topic>dairy cow</topic><topic>Deuterium - analysis</topic><topic>Female</topic><topic>Fermentation - drug effects</topic><topic>Gastrointestinal Contents - chemistry</topic><topic>Gastrointestinal Contents - drug effects</topic><topic>Lactation</topic><topic>methane</topic><topic>Methane - metabolism</topic><topic>Milk - secretion</topic><topic>Propanols - pharmacology</topic><topic>Rumen - metabolism</topic><topic>Rumen - microbiology</topic><topic>rumen fermentation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lopes, J.C.</creatorcontrib><creatorcontrib>de Matos, L.F.</creatorcontrib><creatorcontrib>Harper, M.T.</creatorcontrib><creatorcontrib>Giallongo, F.</creatorcontrib><creatorcontrib>Oh, J.</creatorcontrib><creatorcontrib>Gruen, D.</creatorcontrib><creatorcontrib>Ono, S.</creatorcontrib><creatorcontrib>Kindermann, M.</creatorcontrib><creatorcontrib>Duval, S.</creatorcontrib><creatorcontrib>Hristov, A.N.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of dairy science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lopes, J.C.</au><au>de Matos, L.F.</au><au>Harper, M.T.</au><au>Giallongo, F.</au><au>Oh, J.</au><au>Gruen, D.</au><au>Ono, S.</au><au>Kindermann, M.</au><au>Duval, S.</au><au>Hristov, A.N.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of 3-nitrooxypropanol on methane and hydrogen emissions, methane isotopic signature, and ruminal fermentation in dairy cows</atitle><jtitle>Journal of dairy science</jtitle><addtitle>J Dairy Sci</addtitle><date>2016-07</date><risdate>2016</risdate><volume>99</volume><issue>7</issue><spage>5335</spage><epage>5344</epage><pages>5335-5344</pages><issn>0022-0302</issn><eissn>1525-3198</eissn><abstract>The objective of this crossover experiment was to investigate the effect of a methane inhibitor, 3-nitrooxypropanol (3NOP), on enteric methane emission, methane isotopic composition, and rumen fermentation and microbial profile in lactating dairy cows. The experiment involved 6 ruminally cannulated late-lactation Holstein cows assigned to 2 treatments: control and 3NOP (60 mg/kg of feed dry matter). Compared with the control, 3NOP decreased methane emission by 31% and increased hydrogen emission from undetectable to 1.33 g/d. Methane emissions per kilogram of dry matter intake and milk yield were also decreased 34% by 3NOP. Milk production and composition were not affected by 3NOP, except milk fat concentration was increased compared with the control. Concentrations of total VFA and propionate in ruminal fluid were not affected by treatment, but acetate concentration tended to be lower and acetate-to-propionate ratio was lower for 3NOP compared with the control. The 3NOP decreased the molar proportion of acetate and increase those of propionate, butyrate, valerate, and isovalerate. Deuterium-to-hydrogen ratios of methane and the abundance of 13CH3D were similar between treatments. Compared with the control, minor (4‰) depletion in the 13C/12C ratio was observed for 3NOP. Genus composition of methanogenic archaea (Methanobrevibacter, Methanosphaera, and Methanomicrobium) was not affected by 3NOP, but the proportion of methanogens in the total cell counts tended to be decreased by 3NOP. Prevotella spp., the predominant bacterial genus in ruminal contents in this experiment, was also not affected by 3NOP. Compared with the control, Ruminococcus and Clostridium spp. were decreased and Butyrivibrio spp. was increased by 3NOP. This experiment demonstrated that a substantial inhibition of enteric methane emission by 3NOP in dairy cows was accompanied with increased hydrogen emission and decreased acetate-to-propionate ratio; however, neither an effect on rumen archaeal community composition nor a significant change in the isotope composition of methane was observed.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>27085412</pmid><doi>10.3168/jds.2015-10832</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | 3-nitrooxypropanol Animals Archaea - drug effects Archaea - physiology Carbon Isotopes - analysis Cattle - physiology Cross-Over Studies dairy cow Deuterium - analysis Female Fermentation - drug effects Gastrointestinal Contents - chemistry Gastrointestinal Contents - drug effects Lactation methane Methane - metabolism Milk - secretion Propanols - pharmacology Rumen - metabolism Rumen - microbiology rumen fermentation |
| title | Effect of 3-nitrooxypropanol on methane and hydrogen emissions, methane isotopic signature, and ruminal fermentation in dairy cows |
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