Expression of the prospective mesoderm genes twist, snail, and mef2 in penaeid shrimp
In penaeid shrimp, mesoderm forms from two sources: naupliar mesoderm founder cells, which invaginate during gastrulation, and posterior mesodermal stem cells called mesoteloblasts , which undergo characteristic teloblastic divisions. The primordial mesoteloblast descends from the ventral mesendobla...
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creator | Wei, Jiankai Glaves, Richard Samuel Elliot Sellars, Melony J. Xiang, Jianhai Hertzler, Philip L. |
description | In penaeid shrimp, mesoderm forms from two sources:
naupliar mesoderm
founder cells, which invaginate during gastrulation, and posterior mesodermal stem cells called
mesoteloblasts
, which undergo characteristic teloblastic divisions. The primordial mesoteloblast descends from the ventral mesendoblast, which arrests in cell division at the 32-cell stage and ingresses with its sister dorsal mesendoblast prior to naupliar mesoderm invagination. The naupliar mesoderm forms the muscles of the naupliar appendages (first and second antennae and mandibles), while the mesoteloblasts form the mesoderm, including the muscles, of subsequently formed posterior segments. To better understand the mechanism of mesoderm and muscle formation in penaeid shrimp,
twist
,
snail
, and
mef2
cDNAs were identified from transcriptomes of
Penaeus vannamei
,
P. japonicus
,
P. chinensis
, and
P. monodon
. A single Twist ortholog was found, with strong inferred amino acid conservation across all three species. Multiple Snail protein variants were detected, which clustered in a phylogenetic tree with other decapod crustacean Snail sequences. Two closely-related mef2 variants were found in
P. vannamei
. The developmental mRNA expression of these genes was studied by qPCR in
P. vannamei
embryos, larvae, and postlarvae. Expression of Pv-
twist
and Pv-
snail
began during the limb bud stage and continued through larval stages to the postlarva. Surprisingly, Pv-
mef2
expression was found in all stages from the zygote to the postlarva, with the highest expression in the limb bud and protozoeal stages. The results add comparative data on the development of anterior and posterior mesoderm in malacostracan crustaceans, and should stimulate further studies on mesoderm and muscle development in penaeid shrimp. |
doi_str_mv | 10.1007/s00427-016-0544-5 |
format | Article |
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naupliar mesoderm
founder cells, which invaginate during gastrulation, and posterior mesodermal stem cells called
mesoteloblasts
, which undergo characteristic teloblastic divisions. The primordial mesoteloblast descends from the ventral mesendoblast, which arrests in cell division at the 32-cell stage and ingresses with its sister dorsal mesendoblast prior to naupliar mesoderm invagination. The naupliar mesoderm forms the muscles of the naupliar appendages (first and second antennae and mandibles), while the mesoteloblasts form the mesoderm, including the muscles, of subsequently formed posterior segments. To better understand the mechanism of mesoderm and muscle formation in penaeid shrimp,
twist
,
snail
, and
mef2
cDNAs were identified from transcriptomes of
Penaeus vannamei
,
P. japonicus
,
P. chinensis
, and
P. monodon
. A single Twist ortholog was found, with strong inferred amino acid conservation across all three species. Multiple Snail protein variants were detected, which clustered in a phylogenetic tree with other decapod crustacean Snail sequences. Two closely-related mef2 variants were found in
P. vannamei
. The developmental mRNA expression of these genes was studied by qPCR in
P. vannamei
embryos, larvae, and postlarvae. Expression of Pv-
twist
and Pv-
snail
began during the limb bud stage and continued through larval stages to the postlarva. Surprisingly, Pv-
mef2
expression was found in all stages from the zygote to the postlarva, with the highest expression in the limb bud and protozoeal stages. The results add comparative data on the development of anterior and posterior mesoderm in malacostracan crustaceans, and should stimulate further studies on mesoderm and muscle development in penaeid shrimp.</description><identifier>ISSN: 0949-944X</identifier><identifier>EISSN: 1432-041X</identifier><identifier>DOI: 10.1007/s00427-016-0544-5</identifier><identifier>PMID: 27129985</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Amino Acid Sequence ; Animal Genetics and Genomics ; Animals ; Biomedical and Life Sciences ; Cell Biology ; Developmental Biology ; Evolutionary Biology ; Life Sciences ; Mesoderm - metabolism ; Penaeidae - genetics ; Penaeidae - metabolism ; Plant Genetics and Genomics ; Sequence Alignment ; Short Communication ; Snail Family Transcription Factors - chemistry ; Snail Family Transcription Factors - genetics ; Transcription Factors - chemistry ; Transcription Factors - genetics ; Twist Transcription Factors - chemistry ; Twist Transcription Factors - genetics ; Zoology</subject><ispartof>Development genes and evolution, 2016-07, Vol.226 (4), p.317-324</ispartof><rights>Springer-Verlag Berlin Heidelberg 2016</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c372t-3c2ce24b06630654c3f88dff89c91ffe59696682fb71187ad979005900423cb13</citedby><cites>FETCH-LOGICAL-c372t-3c2ce24b06630654c3f88dff89c91ffe59696682fb71187ad979005900423cb13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00427-016-0544-5$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00427-016-0544-5$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27129985$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wei, Jiankai</creatorcontrib><creatorcontrib>Glaves, Richard Samuel Elliot</creatorcontrib><creatorcontrib>Sellars, Melony J.</creatorcontrib><creatorcontrib>Xiang, Jianhai</creatorcontrib><creatorcontrib>Hertzler, Philip L.</creatorcontrib><title>Expression of the prospective mesoderm genes twist, snail, and mef2 in penaeid shrimp</title><title>Development genes and evolution</title><addtitle>Dev Genes Evol</addtitle><addtitle>Dev Genes Evol</addtitle><description>In penaeid shrimp, mesoderm forms from two sources:
naupliar mesoderm
founder cells, which invaginate during gastrulation, and posterior mesodermal stem cells called
mesoteloblasts
, which undergo characteristic teloblastic divisions. The primordial mesoteloblast descends from the ventral mesendoblast, which arrests in cell division at the 32-cell stage and ingresses with its sister dorsal mesendoblast prior to naupliar mesoderm invagination. The naupliar mesoderm forms the muscles of the naupliar appendages (first and second antennae and mandibles), while the mesoteloblasts form the mesoderm, including the muscles, of subsequently formed posterior segments. To better understand the mechanism of mesoderm and muscle formation in penaeid shrimp,
twist
,
snail
, and
mef2
cDNAs were identified from transcriptomes of
Penaeus vannamei
,
P. japonicus
,
P. chinensis
, and
P. monodon
. A single Twist ortholog was found, with strong inferred amino acid conservation across all three species. Multiple Snail protein variants were detected, which clustered in a phylogenetic tree with other decapod crustacean Snail sequences. Two closely-related mef2 variants were found in
P. vannamei
. The developmental mRNA expression of these genes was studied by qPCR in
P. vannamei
embryos, larvae, and postlarvae. Expression of Pv-
twist
and Pv-
snail
began during the limb bud stage and continued through larval stages to the postlarva. Surprisingly, Pv-
mef2
expression was found in all stages from the zygote to the postlarva, with the highest expression in the limb bud and protozoeal stages. The results add comparative data on the development of anterior and posterior mesoderm in malacostracan crustaceans, and should stimulate further studies on mesoderm and muscle development in penaeid shrimp.</description><subject>Amino Acid Sequence</subject><subject>Animal Genetics and Genomics</subject><subject>Animals</subject><subject>Biomedical and Life Sciences</subject><subject>Cell Biology</subject><subject>Developmental Biology</subject><subject>Evolutionary Biology</subject><subject>Life Sciences</subject><subject>Mesoderm - metabolism</subject><subject>Penaeidae - genetics</subject><subject>Penaeidae - metabolism</subject><subject>Plant Genetics and Genomics</subject><subject>Sequence Alignment</subject><subject>Short Communication</subject><subject>Snail Family Transcription Factors - chemistry</subject><subject>Snail Family Transcription Factors - genetics</subject><subject>Transcription Factors - chemistry</subject><subject>Transcription Factors - genetics</subject><subject>Twist Transcription Factors - chemistry</subject><subject>Twist Transcription Factors - genetics</subject><subject>Zoology</subject><issn>0949-944X</issn><issn>1432-041X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp1kMtKxDAUhoMoOo4-gBsJuHFhNbc2zVIGbyC4cWB2oU1PtDJNa0_Hy9uboaOI4CqBfOfPfz5Cjjg754zpC2RMCZ0wniUsVSpJt8iEKykSpvhim0yYUSYxSi32yD7iC2NcGJnukj2h483k6YTMrz66HhDrNtDW0-EZaNe32IEb6jegDWBbQd_QJwiAdHivcTijGIp6eUaLUEXAC1oH2kEooK4oPvd10x2QHV8sEQ4355TMr68eZ7fJ_cPN3ezyPnFSiyGRTjgQqmRZJlmWKid9nlfe58YZ7j2kJjNZlgtfas5zXVRGG8ZSs15bupLLKTkdc2Pn1xXgYJsaHSyXRYB2hZZro6MYafKInvxBX9pVH2K7NZUpKWOHSPGRclEC9uBtF_cp-k_LmV07t6NzG53btXObxpnjTfKqbKD6mfiWHAExAhifwhP0v77-N_ULIB-Kjg</recordid><startdate>20160701</startdate><enddate>20160701</enddate><creator>Wei, Jiankai</creator><creator>Glaves, Richard Samuel Elliot</creator><creator>Sellars, Melony J.</creator><creator>Xiang, Jianhai</creator><creator>Hertzler, Philip L.</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QP</scope><scope>7SS</scope><scope>7TK</scope><scope>7TM</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20160701</creationdate><title>Expression of the prospective mesoderm genes twist, snail, and mef2 in penaeid shrimp</title><author>Wei, Jiankai ; Glaves, Richard Samuel Elliot ; Sellars, Melony J. ; Xiang, Jianhai ; Hertzler, Philip L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c372t-3c2ce24b06630654c3f88dff89c91ffe59696682fb71187ad979005900423cb13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Amino Acid Sequence</topic><topic>Animal Genetics and Genomics</topic><topic>Animals</topic><topic>Biomedical and Life Sciences</topic><topic>Cell Biology</topic><topic>Developmental Biology</topic><topic>Evolutionary Biology</topic><topic>Life Sciences</topic><topic>Mesoderm - metabolism</topic><topic>Penaeidae - genetics</topic><topic>Penaeidae - metabolism</topic><topic>Plant Genetics and Genomics</topic><topic>Sequence Alignment</topic><topic>Short Communication</topic><topic>Snail Family Transcription Factors - chemistry</topic><topic>Snail Family Transcription Factors - genetics</topic><topic>Transcription Factors - chemistry</topic><topic>Transcription Factors - genetics</topic><topic>Twist Transcription Factors - chemistry</topic><topic>Twist Transcription Factors - genetics</topic><topic>Zoology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wei, Jiankai</creatorcontrib><creatorcontrib>Glaves, Richard Samuel Elliot</creatorcontrib><creatorcontrib>Sellars, Melony J.</creatorcontrib><creatorcontrib>Xiang, Jianhai</creatorcontrib><creatorcontrib>Hertzler, Philip L.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Development genes and evolution</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wei, Jiankai</au><au>Glaves, Richard Samuel Elliot</au><au>Sellars, Melony J.</au><au>Xiang, Jianhai</au><au>Hertzler, Philip L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression of the prospective mesoderm genes twist, snail, and mef2 in penaeid shrimp</atitle><jtitle>Development genes and evolution</jtitle><stitle>Dev Genes Evol</stitle><addtitle>Dev Genes Evol</addtitle><date>2016-07-01</date><risdate>2016</risdate><volume>226</volume><issue>4</issue><spage>317</spage><epage>324</epage><pages>317-324</pages><issn>0949-944X</issn><eissn>1432-041X</eissn><abstract>In penaeid shrimp, mesoderm forms from two sources:
naupliar mesoderm
founder cells, which invaginate during gastrulation, and posterior mesodermal stem cells called
mesoteloblasts
, which undergo characteristic teloblastic divisions. The primordial mesoteloblast descends from the ventral mesendoblast, which arrests in cell division at the 32-cell stage and ingresses with its sister dorsal mesendoblast prior to naupliar mesoderm invagination. The naupliar mesoderm forms the muscles of the naupliar appendages (first and second antennae and mandibles), while the mesoteloblasts form the mesoderm, including the muscles, of subsequently formed posterior segments. To better understand the mechanism of mesoderm and muscle formation in penaeid shrimp,
twist
,
snail
, and
mef2
cDNAs were identified from transcriptomes of
Penaeus vannamei
,
P. japonicus
,
P. chinensis
, and
P. monodon
. A single Twist ortholog was found, with strong inferred amino acid conservation across all three species. Multiple Snail protein variants were detected, which clustered in a phylogenetic tree with other decapod crustacean Snail sequences. Two closely-related mef2 variants were found in
P. vannamei
. The developmental mRNA expression of these genes was studied by qPCR in
P. vannamei
embryos, larvae, and postlarvae. Expression of Pv-
twist
and Pv-
snail
began during the limb bud stage and continued through larval stages to the postlarva. Surprisingly, Pv-
mef2
expression was found in all stages from the zygote to the postlarva, with the highest expression in the limb bud and protozoeal stages. The results add comparative data on the development of anterior and posterior mesoderm in malacostracan crustaceans, and should stimulate further studies on mesoderm and muscle development in penaeid shrimp.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>27129985</pmid><doi>10.1007/s00427-016-0544-5</doi><tpages>8</tpages></addata></record> |
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subjects | Amino Acid Sequence Animal Genetics and Genomics Animals Biomedical and Life Sciences Cell Biology Developmental Biology Evolutionary Biology Life Sciences Mesoderm - metabolism Penaeidae - genetics Penaeidae - metabolism Plant Genetics and Genomics Sequence Alignment Short Communication Snail Family Transcription Factors - chemistry Snail Family Transcription Factors - genetics Transcription Factors - chemistry Transcription Factors - genetics Twist Transcription Factors - chemistry Twist Transcription Factors - genetics Zoology |
title | Expression of the prospective mesoderm genes twist, snail, and mef2 in penaeid shrimp |
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