Expression of B/K protein in the hippocampus of kainate-induced rat seizure model
B/K protein is a newly identified member of double C2-like domain protein family. We examined the expression of B/K protein in the hippocampus of kainate-induced rat seizure model. Intraperitoneal injection of kainate increased the immunoreactivity to B/K protein in the CA1 to CA3 of the hippocampus...
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| Veröffentlicht in: | Brain research 2004-03, Vol.999 (2), p.203-211 |
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| creator | Jang, Yoon-Seong Lee, Mun-Yong Choi, Sung-Ho Kim, Mi-Young Chin, Hemin Jeong, Seong-Whan Kim, In-Kyung Kwon, Oh-Joo |
| description | B/K protein is a newly identified member of double C2-like domain protein family. We examined the expression of B/K protein in the hippocampus of kainate-induced rat seizure model. Intraperitoneal injection of kainate increased the immunoreactivity to B/K protein in the CA1 to CA3 of the hippocampus. B/K protein expression began to increase at 6 h, reached the maximum at 12 h, and then returned nearly to the normal level at 72 h after the injection of kainate (12 mg/kg), and it was also dependent on the dose of kainate between 4 and 16 mg/kg. In electron microscopic and subcellular fractionation studies, B/K protein was localized in the endoplasmic reticulum (ER) of the hippocampus. Kainate also induced the expression of BiP, a typical ER stress marker protein, in the hippocampus and the cortex, and it was coexpressed with B/K protein. Moreover, thapsigargin-induced ER stress caused upregulation of B/K protein expression in PC12 cells. In conclusion, our data showing the induction of both B/K protein expression and ER stress response in the hippocampus of kainate seizure model, and ER-specific expression and ER stress-induced expression of B/K strongly suggest the possible role of B/K protein in epileptogenesis or epilepsy-induced neuronal damage. |
| doi_str_mv | 10.1016/j.brainres.2003.11.047 |
| format | Article |
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We examined the expression of B/K protein in the hippocampus of kainate-induced rat seizure model. Intraperitoneal injection of kainate increased the immunoreactivity to B/K protein in the CA1 to CA3 of the hippocampus. B/K protein expression began to increase at 6 h, reached the maximum at 12 h, and then returned nearly to the normal level at 72 h after the injection of kainate (12 mg/kg), and it was also dependent on the dose of kainate between 4 and 16 mg/kg. In electron microscopic and subcellular fractionation studies, B/K protein was localized in the endoplasmic reticulum (ER) of the hippocampus. Kainate also induced the expression of BiP, a typical ER stress marker protein, in the hippocampus and the cortex, and it was coexpressed with B/K protein. Moreover, thapsigargin-induced ER stress caused upregulation of B/K protein expression in PC12 cells. In conclusion, our data showing the induction of both B/K protein expression and ER stress response in the hippocampus of kainate seizure model, and ER-specific expression and ER stress-induced expression of B/K strongly suggest the possible role of B/K protein in epileptogenesis or epilepsy-induced neuronal damage.</description><identifier>ISSN: 0006-8993</identifier><identifier>EISSN: 1872-6240</identifier><identifier>DOI: 10.1016/j.brainres.2003.11.047</identifier><identifier>PMID: 14759499</identifier><identifier>CODEN: BRREAP</identifier><language>eng</language><publisher>London: Elsevier B.V</publisher><subject>Animals ; Biological and medical sciences ; Carrier Proteins - metabolism ; Cerebral Cortex - metabolism ; Cerebral Cortex - physiopathology ; Disease Models, Animal ; Dose-Response Relationship, Drug ; Endoplasmic reticulum ; Endoplasmic Reticulum - drug effects ; Endoplasmic Reticulum - metabolism ; Endoplasmic Reticulum - ultrastructure ; Enzyme Inhibitors - pharmacology ; Epilepsy - chemically induced ; Epilepsy - metabolism ; Epilepsy - physiopathology ; Excitatory Amino Acid Agonists ; Headache. Facial pains. Syncopes. Epilepsia. Intracranial hypertension. Brain oedema. Cerebral palsy ; Heat-Shock Proteins ; Hippocampus - metabolism ; Hippocampus - physiopathology ; Hippocampus - ultrastructure ; Immunohistochemistry ; Kainic Acid ; Male ; Medical sciences ; Microscopy, Electron ; Molecular Chaperones - metabolism ; Nerve Tissue Proteins - biosynthesis ; Nervous system (semeiology, syndromes) ; Neurology ; Neurons - drug effects ; Neurons - metabolism ; Neurons - ultrastructure ; Neurotoxicity ; PC12 Cells ; Rats ; Rats, Sprague-Dawley ; Reaction Time - drug effects ; Reaction Time - physiology ; Stress, Physiological - metabolism ; Stress, Physiological - physiopathology ; Synaptotagmins ; Up-Regulation - drug effects ; Up-Regulation - physiology</subject><ispartof>Brain research, 2004-03, Vol.999 (2), p.203-211</ispartof><rights>2003</rights><rights>2004 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c491t-931602e16bfe10ae5f1dadb2ca0396870fd9bae94ada34c918373bfed5404cc13</citedby><cites>FETCH-LOGICAL-c491t-931602e16bfe10ae5f1dadb2ca0396870fd9bae94ada34c918373bfed5404cc13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.brainres.2003.11.047$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15479980$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/14759499$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jang, Yoon-Seong</creatorcontrib><creatorcontrib>Lee, Mun-Yong</creatorcontrib><creatorcontrib>Choi, Sung-Ho</creatorcontrib><creatorcontrib>Kim, Mi-Young</creatorcontrib><creatorcontrib>Chin, Hemin</creatorcontrib><creatorcontrib>Jeong, Seong-Whan</creatorcontrib><creatorcontrib>Kim, In-Kyung</creatorcontrib><creatorcontrib>Kwon, Oh-Joo</creatorcontrib><title>Expression of B/K protein in the hippocampus of kainate-induced rat seizure model</title><title>Brain research</title><addtitle>Brain Res</addtitle><description>B/K protein is a newly identified member of double C2-like domain protein family. We examined the expression of B/K protein in the hippocampus of kainate-induced rat seizure model. Intraperitoneal injection of kainate increased the immunoreactivity to B/K protein in the CA1 to CA3 of the hippocampus. B/K protein expression began to increase at 6 h, reached the maximum at 12 h, and then returned nearly to the normal level at 72 h after the injection of kainate (12 mg/kg), and it was also dependent on the dose of kainate between 4 and 16 mg/kg. In electron microscopic and subcellular fractionation studies, B/K protein was localized in the endoplasmic reticulum (ER) of the hippocampus. Kainate also induced the expression of BiP, a typical ER stress marker protein, in the hippocampus and the cortex, and it was coexpressed with B/K protein. Moreover, thapsigargin-induced ER stress caused upregulation of B/K protein expression in PC12 cells. In conclusion, our data showing the induction of both B/K protein expression and ER stress response in the hippocampus of kainate seizure model, and ER-specific expression and ER stress-induced expression of B/K strongly suggest the possible role of B/K protein in epileptogenesis or epilepsy-induced neuronal damage.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Carrier Proteins - metabolism</subject><subject>Cerebral Cortex - metabolism</subject><subject>Cerebral Cortex - physiopathology</subject><subject>Disease Models, Animal</subject><subject>Dose-Response Relationship, Drug</subject><subject>Endoplasmic reticulum</subject><subject>Endoplasmic Reticulum - drug effects</subject><subject>Endoplasmic Reticulum - metabolism</subject><subject>Endoplasmic Reticulum - ultrastructure</subject><subject>Enzyme Inhibitors - pharmacology</subject><subject>Epilepsy - chemically induced</subject><subject>Epilepsy - metabolism</subject><subject>Epilepsy - physiopathology</subject><subject>Excitatory Amino Acid Agonists</subject><subject>Headache. Facial pains. Syncopes. Epilepsia. Intracranial hypertension. Brain oedema. Cerebral palsy</subject><subject>Heat-Shock Proteins</subject><subject>Hippocampus - metabolism</subject><subject>Hippocampus - physiopathology</subject><subject>Hippocampus - ultrastructure</subject><subject>Immunohistochemistry</subject><subject>Kainic Acid</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Microscopy, Electron</subject><subject>Molecular Chaperones - metabolism</subject><subject>Nerve Tissue Proteins - biosynthesis</subject><subject>Nervous system (semeiology, syndromes)</subject><subject>Neurology</subject><subject>Neurons - drug effects</subject><subject>Neurons - metabolism</subject><subject>Neurons - ultrastructure</subject><subject>Neurotoxicity</subject><subject>PC12 Cells</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Reaction Time - drug effects</subject><subject>Reaction Time - physiology</subject><subject>Stress, Physiological - metabolism</subject><subject>Stress, Physiological - physiopathology</subject><subject>Synaptotagmins</subject><subject>Up-Regulation - drug effects</subject><subject>Up-Regulation - physiology</subject><issn>0006-8993</issn><issn>1872-6240</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1r3DAQhkVoSLZp_0Lwpb3ZmbHkD93SLmkbshAC7VnI0pjVxl-V7JDm10fLbskxMDAMPPPO8DB2iZAhYHm1yxqv3eApZDkAzxAzENUJW2Fd5WmZC_jAVgBQprWU_Jx9DGEXR84lnLFzFFUhhZQr9nDzPMWQ4MYhGdvk-9VdMvlxJjckseYtJVs3TaPR_bSEPfEYr-qZUjfYxZBNvJ6TQO5l8ZT0o6XuEzttdRfo87FfsD8_bn6vf6Wb-5-362-b1AiJcyo5lpATlk1LCJqKFq22TW40cFnWFbRWNpqk0FZzYSTWvOKRtYUAYQzyC_b1kBv__btQmFXvgqGu0wONS1BYxRheFhEsD6DxYwieWjV512v_TyGovUy1U_9lqr1MhaiizLh4ebywND3Zt7WjvQh8OQI6GN21Xg_GhTeuEJWUNUTu-sBR9PHkyKtgHA1Rn_NkZmVH994vr1V0luU</recordid><startdate>20040305</startdate><enddate>20040305</enddate><creator>Jang, Yoon-Seong</creator><creator>Lee, Mun-Yong</creator><creator>Choi, Sung-Ho</creator><creator>Kim, Mi-Young</creator><creator>Chin, Hemin</creator><creator>Jeong, Seong-Whan</creator><creator>Kim, In-Kyung</creator><creator>Kwon, Oh-Joo</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope></search><sort><creationdate>20040305</creationdate><title>Expression of B/K protein in the hippocampus of kainate-induced rat seizure model</title><author>Jang, Yoon-Seong ; Lee, Mun-Yong ; Choi, Sung-Ho ; Kim, Mi-Young ; Chin, Hemin ; Jeong, Seong-Whan ; Kim, In-Kyung ; Kwon, Oh-Joo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c491t-931602e16bfe10ae5f1dadb2ca0396870fd9bae94ada34c918373bfed5404cc13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Carrier Proteins - metabolism</topic><topic>Cerebral Cortex - metabolism</topic><topic>Cerebral Cortex - physiopathology</topic><topic>Disease Models, Animal</topic><topic>Dose-Response Relationship, Drug</topic><topic>Endoplasmic reticulum</topic><topic>Endoplasmic Reticulum - drug effects</topic><topic>Endoplasmic Reticulum - metabolism</topic><topic>Endoplasmic Reticulum - ultrastructure</topic><topic>Enzyme Inhibitors - pharmacology</topic><topic>Epilepsy - chemically induced</topic><topic>Epilepsy - metabolism</topic><topic>Epilepsy - physiopathology</topic><topic>Excitatory Amino Acid Agonists</topic><topic>Headache. Facial pains. Syncopes. Epilepsia. Intracranial hypertension. Brain oedema. Cerebral palsy</topic><topic>Heat-Shock Proteins</topic><topic>Hippocampus - metabolism</topic><topic>Hippocampus - physiopathology</topic><topic>Hippocampus - ultrastructure</topic><topic>Immunohistochemistry</topic><topic>Kainic Acid</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Microscopy, Electron</topic><topic>Molecular Chaperones - metabolism</topic><topic>Nerve Tissue Proteins - biosynthesis</topic><topic>Nervous system (semeiology, syndromes)</topic><topic>Neurology</topic><topic>Neurons - drug effects</topic><topic>Neurons - metabolism</topic><topic>Neurons - ultrastructure</topic><topic>Neurotoxicity</topic><topic>PC12 Cells</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Reaction Time - drug effects</topic><topic>Reaction Time - physiology</topic><topic>Stress, Physiological - metabolism</topic><topic>Stress, Physiological - physiopathology</topic><topic>Synaptotagmins</topic><topic>Up-Regulation - drug effects</topic><topic>Up-Regulation - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jang, Yoon-Seong</creatorcontrib><creatorcontrib>Lee, Mun-Yong</creatorcontrib><creatorcontrib>Choi, Sung-Ho</creatorcontrib><creatorcontrib>Kim, Mi-Young</creatorcontrib><creatorcontrib>Chin, Hemin</creatorcontrib><creatorcontrib>Jeong, Seong-Whan</creatorcontrib><creatorcontrib>Kim, In-Kyung</creatorcontrib><creatorcontrib>Kwon, Oh-Joo</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><jtitle>Brain research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jang, Yoon-Seong</au><au>Lee, Mun-Yong</au><au>Choi, Sung-Ho</au><au>Kim, Mi-Young</au><au>Chin, Hemin</au><au>Jeong, Seong-Whan</au><au>Kim, In-Kyung</au><au>Kwon, Oh-Joo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression of B/K protein in the hippocampus of kainate-induced rat seizure model</atitle><jtitle>Brain research</jtitle><addtitle>Brain Res</addtitle><date>2004-03-05</date><risdate>2004</risdate><volume>999</volume><issue>2</issue><spage>203</spage><epage>211</epage><pages>203-211</pages><issn>0006-8993</issn><eissn>1872-6240</eissn><coden>BRREAP</coden><abstract>B/K protein is a newly identified member of double C2-like domain protein family. We examined the expression of B/K protein in the hippocampus of kainate-induced rat seizure model. Intraperitoneal injection of kainate increased the immunoreactivity to B/K protein in the CA1 to CA3 of the hippocampus. B/K protein expression began to increase at 6 h, reached the maximum at 12 h, and then returned nearly to the normal level at 72 h after the injection of kainate (12 mg/kg), and it was also dependent on the dose of kainate between 4 and 16 mg/kg. In electron microscopic and subcellular fractionation studies, B/K protein was localized in the endoplasmic reticulum (ER) of the hippocampus. Kainate also induced the expression of BiP, a typical ER stress marker protein, in the hippocampus and the cortex, and it was coexpressed with B/K protein. Moreover, thapsigargin-induced ER stress caused upregulation of B/K protein expression in PC12 cells. In conclusion, our data showing the induction of both B/K protein expression and ER stress response in the hippocampus of kainate seizure model, and ER-specific expression and ER stress-induced expression of B/K strongly suggest the possible role of B/K protein in epileptogenesis or epilepsy-induced neuronal damage.</abstract><cop>London</cop><cop>Amsterdam</cop><cop>New York, NY</cop><pub>Elsevier B.V</pub><pmid>14759499</pmid><doi>10.1016/j.brainres.2003.11.047</doi><tpages>9</tpages></addata></record> |
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| subjects | Animals Biological and medical sciences Carrier Proteins - metabolism Cerebral Cortex - metabolism Cerebral Cortex - physiopathology Disease Models, Animal Dose-Response Relationship, Drug Endoplasmic reticulum Endoplasmic Reticulum - drug effects Endoplasmic Reticulum - metabolism Endoplasmic Reticulum - ultrastructure Enzyme Inhibitors - pharmacology Epilepsy - chemically induced Epilepsy - metabolism Epilepsy - physiopathology Excitatory Amino Acid Agonists Headache. Facial pains. Syncopes. Epilepsia. Intracranial hypertension. Brain oedema. Cerebral palsy Heat-Shock Proteins Hippocampus - metabolism Hippocampus - physiopathology Hippocampus - ultrastructure Immunohistochemistry Kainic Acid Male Medical sciences Microscopy, Electron Molecular Chaperones - metabolism Nerve Tissue Proteins - biosynthesis Nervous system (semeiology, syndromes) Neurology Neurons - drug effects Neurons - metabolism Neurons - ultrastructure Neurotoxicity PC12 Cells Rats Rats, Sprague-Dawley Reaction Time - drug effects Reaction Time - physiology Stress, Physiological - metabolism Stress, Physiological - physiopathology Synaptotagmins Up-Regulation - drug effects Up-Regulation - physiology |
| title | Expression of B/K protein in the hippocampus of kainate-induced rat seizure model |
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