3C-like Proteinase from SARS Coronavirus Catalyzes Substrate Hydrolysis by a General Base Mechanism

SARS 3C-like proteinase has been proposed to be a key enzyme for drug design against SARS. Lack of a suitable assay has been a major hindrance for enzyme kinetic studies and a large-scale inhibitor screen for SARS 3CL proteinase. Since SARS 3CL proteinase belongs to the cysteine protease family (fam...

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Veröffentlicht in:	Biochemistry (Easton) 2004-04, Vol.43 (15), p.4568-4574
Hauptverfasser:	Huang, Changkang, Wei, Ping, Fan, Keqiang, Liu, Ying, Lai, Luhua
Format:	Artikel
Sprache:	eng
Schlagworte:	Alanine - genetics Amino Acid Substitution - genetics Catalysis Colorimetry - methods Cysteine - genetics Cysteine Endopeptidases Deuterium Exchange Measurement Endopeptidases - chemistry Endopeptidases - genetics Histidine - genetics Hydrogen-Ion Concentration Hydrolysis Kinetics Mutagenesis, Site-Directed SARS coronavirus SARS Virus - enzymology SARS Virus - genetics Serine - genetics Solvents Substrate Specificity - genetics Temperature Thermodynamics Viral Proteins - chemistry Viral Proteins - genetics
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