Mechanisms of the insecticidal action of TEL (Talisia esculenta lectin) against Callosobruchus maculatus (Coleoptera: Bruchidae)
Plant lectins have insecticidal activity that is probably mediated through their ability to bind carbohydrates. To examine the influence of sugars on the insecticidal activity of a lectin from Talisia esculenta seeds (TEL), the lectin was mixed with mannose, glucose, or mannose plus glucose. Mannose...
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| Veröffentlicht in: | Archives of insect biochemistry and physiology 2004-06, Vol.56 (2), p.84-96 |
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| creator | Macedo, M.L.R Castro, M.M. de Freire, M. das G.M |
| description | Plant lectins have insecticidal activity that is probably mediated through their ability to bind carbohydrates. To examine the influence of sugars on the insecticidal activity of a lectin from Talisia esculenta seeds (TEL), the lectin was mixed with mannose, glucose, or mannose plus glucose. Mannose abolished the insecticidal activity. Affinity chromatography showed that TEL bound to midgut proteins of the insect Callosobruchus maculatus. Immunoblotting showed that TEL recognized some proteins, probably glycoproteins, present in the midgut membrane of this insect. The principal proteases responsible for digestive proteolysis in fourth instar larvae of C. maculatus were purified by chromatography on activated thiol-Sepharose. These purified proteases were unable to digest TEL after a 15-h incubation. These results suggest that the insecticidal activity of TEL involves a specific carbohydrate-lectin interaction with glycoconjugates on the surface of digestive tract epithelial cells, as well as binding to assimilatory glycoproteins present in midgut extracts and resistance to enzymatic digestion by cysteine proteinases. |
| doi_str_mv | 10.1002/arch.10145 |
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To examine the influence of sugars on the insecticidal activity of a lectin from Talisia esculenta seeds (TEL), the lectin was mixed with mannose, glucose, or mannose plus glucose. Mannose abolished the insecticidal activity. Affinity chromatography showed that TEL bound to midgut proteins of the insect Callosobruchus maculatus. Immunoblotting showed that TEL recognized some proteins, probably glycoproteins, present in the midgut membrane of this insect. The principal proteases responsible for digestive proteolysis in fourth instar larvae of C. maculatus were purified by chromatography on activated thiol-Sepharose. These purified proteases were unable to digest TEL after a 15-h incubation. These results suggest that the insecticidal activity of TEL involves a specific carbohydrate-lectin interaction with glycoconjugates on the surface of digestive tract epithelial cells, as well as binding to assimilatory glycoproteins present in midgut extracts and resistance to enzymatic digestion by cysteine proteinases.</description><identifier>ISSN: 0739-4462</identifier><identifier>EISSN: 1520-6327</identifier><identifier>DOI: 10.1002/arch.10145</identifier><identifier>PMID: 15146543</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Animals ; Biological Assay - methods ; Body Weight ; Bruchidae ; Callosobruchus maculatus ; carbohydrate binding ; Carbohydrates - pharmacology ; cell membranes ; Coleoptera - drug effects ; Coleoptera - enzymology ; Cysteine Endopeptidases - metabolism ; cysteine proteinases ; digestion ; Digestive System - enzymology ; enzyme activity ; epithelial cells ; glucose ; glycoconjugates ; insect control ; insecticidal properties ; insecticide ; Insecticides - antagonists & inhibitors ; Insecticides - metabolism ; Insecticides - pharmacology ; Intestinal Mucosa - metabolism ; Larva - drug effects ; lectin ; lectins ; mannose ; mechanism of action ; midgut ; Plant Lectins - antagonists & inhibitors ; Plant Lectins - metabolism ; Plant Lectins - pharmacology ; plant-insect interaction ; protein binding ; proteolysis ; resistance to digestion ; Sapindaceae ; Sapindaceae - chemistry ; seeds ; Seeds - chemistry ; storage insects ; Survival Analysis ; Talisia esculenta ; toxicology</subject><ispartof>Archives of insect biochemistry and physiology, 2004-06, Vol.56 (2), p.84-96</ispartof><rights>Copyright © 2004 Wiley‐Liss, Inc.</rights><rights>Copyright 2004 Wiley-Liss, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3875-3b3f07820d1f35938fb6ca3fc468ec369001f394fb1092318671a375eccd84533</citedby><cites>FETCH-LOGICAL-c3875-3b3f07820d1f35938fb6ca3fc468ec369001f394fb1092318671a375eccd84533</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Farch.10145$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Farch.10145$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15146543$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Macedo, M.L.R</creatorcontrib><creatorcontrib>Castro, M.M. de</creatorcontrib><creatorcontrib>Freire, M. das G.M</creatorcontrib><title>Mechanisms of the insecticidal action of TEL (Talisia esculenta lectin) against Callosobruchus maculatus (Coleoptera: Bruchidae)</title><title>Archives of insect biochemistry and physiology</title><addtitle>Arch. Insect Biochem. Physiol</addtitle><description>Plant lectins have insecticidal activity that is probably mediated through their ability to bind carbohydrates. To examine the influence of sugars on the insecticidal activity of a lectin from Talisia esculenta seeds (TEL), the lectin was mixed with mannose, glucose, or mannose plus glucose. Mannose abolished the insecticidal activity. Affinity chromatography showed that TEL bound to midgut proteins of the insect Callosobruchus maculatus. Immunoblotting showed that TEL recognized some proteins, probably glycoproteins, present in the midgut membrane of this insect. The principal proteases responsible for digestive proteolysis in fourth instar larvae of C. maculatus were purified by chromatography on activated thiol-Sepharose. These purified proteases were unable to digest TEL after a 15-h incubation. These results suggest that the insecticidal activity of TEL involves a specific carbohydrate-lectin interaction with glycoconjugates on the surface of digestive tract epithelial cells, as well as binding to assimilatory glycoproteins present in midgut extracts and resistance to enzymatic digestion by cysteine proteinases.</description><subject>Animals</subject><subject>Biological Assay - methods</subject><subject>Body Weight</subject><subject>Bruchidae</subject><subject>Callosobruchus maculatus</subject><subject>carbohydrate binding</subject><subject>Carbohydrates - pharmacology</subject><subject>cell membranes</subject><subject>Coleoptera - drug effects</subject><subject>Coleoptera - enzymology</subject><subject>Cysteine Endopeptidases - metabolism</subject><subject>cysteine proteinases</subject><subject>digestion</subject><subject>Digestive System - enzymology</subject><subject>enzyme activity</subject><subject>epithelial cells</subject><subject>glucose</subject><subject>glycoconjugates</subject><subject>insect control</subject><subject>insecticidal properties</subject><subject>insecticide</subject><subject>Insecticides - antagonists & inhibitors</subject><subject>Insecticides - metabolism</subject><subject>Insecticides - pharmacology</subject><subject>Intestinal Mucosa - metabolism</subject><subject>Larva - drug effects</subject><subject>lectin</subject><subject>lectins</subject><subject>mannose</subject><subject>mechanism of action</subject><subject>midgut</subject><subject>Plant Lectins - antagonists & inhibitors</subject><subject>Plant Lectins - metabolism</subject><subject>Plant Lectins - pharmacology</subject><subject>plant-insect interaction</subject><subject>protein binding</subject><subject>proteolysis</subject><subject>resistance to digestion</subject><subject>Sapindaceae</subject><subject>Sapindaceae - chemistry</subject><subject>seeds</subject><subject>Seeds - chemistry</subject><subject>storage insects</subject><subject>Survival Analysis</subject><subject>Talisia esculenta</subject><subject>toxicology</subject><issn>0739-4462</issn><issn>1520-6327</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kcFu1DAYhC0EotvChQcAn1CLFLDj2Em4tVHpIrYgYKtKXKw_Xrvr4sSLnQh649FxyAI3Th5pvhnLY4SeUPKSEpK_gqC2SdGC30MLynOSCZaX99GClKzOikLkB-gwxltCSC1o9RAdUE4LwQu2QD8vtdpCb2MXsTd42Gps-6jVYJXdgMOQlO8na32-wsdrcDZawDqq0el-AOwmtj_BcAMpOOAGnPPRt2FU2zHiDhIIQ1LHjXfa7wYd4DU-m-x0gT55hB4YcFE_3p9H6OrN-bpZZqsPF2-b01WmWFXyjLXMkLLKyYYaxmtWmVYoYEYVotKKiZqQZNSFaSmpc0YrUVJgJddKbaqCM3aEns-9u-C_jToOsrNRaeeg136Mkpa1EDSfwBczqIKPMWgjd8F2EO4kJXLaW057y997J_jpvnVsO735h-4HTgCdge_W6bv_VMnTT83yT2k2Z2wc9I-_GQhfpSjTm-T1-wv5hV--q5cf1_I68c9m3oCXcBNslFefc0JZ-m9WVTlnvwC0NqN9</recordid><startdate>200406</startdate><enddate>200406</enddate><creator>Macedo, M.L.R</creator><creator>Castro, M.M. de</creator><creator>Freire, M. das G.M</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><scope>FBQ</scope><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SS</scope></search><sort><creationdate>200406</creationdate><title>Mechanisms of the insecticidal action of TEL (Talisia esculenta lectin) against Callosobruchus maculatus (Coleoptera: Bruchidae)</title><author>Macedo, M.L.R ; Castro, M.M. de ; Freire, M. das G.M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3875-3b3f07820d1f35938fb6ca3fc468ec369001f394fb1092318671a375eccd84533</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Animals</topic><topic>Biological Assay - methods</topic><topic>Body Weight</topic><topic>Bruchidae</topic><topic>Callosobruchus maculatus</topic><topic>carbohydrate binding</topic><topic>Carbohydrates - pharmacology</topic><topic>cell membranes</topic><topic>Coleoptera - drug effects</topic><topic>Coleoptera - enzymology</topic><topic>Cysteine Endopeptidases - metabolism</topic><topic>cysteine proteinases</topic><topic>digestion</topic><topic>Digestive System - enzymology</topic><topic>enzyme activity</topic><topic>epithelial cells</topic><topic>glucose</topic><topic>glycoconjugates</topic><topic>insect control</topic><topic>insecticidal properties</topic><topic>insecticide</topic><topic>Insecticides - antagonists & inhibitors</topic><topic>Insecticides - metabolism</topic><topic>Insecticides - pharmacology</topic><topic>Intestinal Mucosa - metabolism</topic><topic>Larva - drug effects</topic><topic>lectin</topic><topic>lectins</topic><topic>mannose</topic><topic>mechanism of action</topic><topic>midgut</topic><topic>Plant Lectins - antagonists & inhibitors</topic><topic>Plant Lectins - metabolism</topic><topic>Plant Lectins - pharmacology</topic><topic>plant-insect interaction</topic><topic>protein binding</topic><topic>proteolysis</topic><topic>resistance to digestion</topic><topic>Sapindaceae</topic><topic>Sapindaceae - chemistry</topic><topic>seeds</topic><topic>Seeds - chemistry</topic><topic>storage insects</topic><topic>Survival Analysis</topic><topic>Talisia esculenta</topic><topic>toxicology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Macedo, M.L.R</creatorcontrib><creatorcontrib>Castro, M.M. de</creatorcontrib><creatorcontrib>Freire, M. das G.M</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Entomology Abstracts (Full archive)</collection><jtitle>Archives of insect biochemistry and physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Macedo, M.L.R</au><au>Castro, M.M. de</au><au>Freire, M. das G.M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Mechanisms of the insecticidal action of TEL (Talisia esculenta lectin) against Callosobruchus maculatus (Coleoptera: Bruchidae)</atitle><jtitle>Archives of insect biochemistry and physiology</jtitle><addtitle>Arch. Insect Biochem. Physiol</addtitle><date>2004-06</date><risdate>2004</risdate><volume>56</volume><issue>2</issue><spage>84</spage><epage>96</epage><pages>84-96</pages><issn>0739-4462</issn><eissn>1520-6327</eissn><abstract>Plant lectins have insecticidal activity that is probably mediated through their ability to bind carbohydrates. To examine the influence of sugars on the insecticidal activity of a lectin from Talisia esculenta seeds (TEL), the lectin was mixed with mannose, glucose, or mannose plus glucose. Mannose abolished the insecticidal activity. Affinity chromatography showed that TEL bound to midgut proteins of the insect Callosobruchus maculatus. Immunoblotting showed that TEL recognized some proteins, probably glycoproteins, present in the midgut membrane of this insect. The principal proteases responsible for digestive proteolysis in fourth instar larvae of C. maculatus were purified by chromatography on activated thiol-Sepharose. These purified proteases were unable to digest TEL after a 15-h incubation. These results suggest that the insecticidal activity of TEL involves a specific carbohydrate-lectin interaction with glycoconjugates on the surface of digestive tract epithelial cells, as well as binding to assimilatory glycoproteins present in midgut extracts and resistance to enzymatic digestion by cysteine proteinases.</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>15146543</pmid><doi>10.1002/arch.10145</doi><tpages>13</tpages></addata></record> |
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| subjects | Animals Biological Assay - methods Body Weight Bruchidae Callosobruchus maculatus carbohydrate binding Carbohydrates - pharmacology cell membranes Coleoptera - drug effects Coleoptera - enzymology Cysteine Endopeptidases - metabolism cysteine proteinases digestion Digestive System - enzymology enzyme activity epithelial cells glucose glycoconjugates insect control insecticidal properties insecticide Insecticides - antagonists & inhibitors Insecticides - metabolism Insecticides - pharmacology Intestinal Mucosa - metabolism Larva - drug effects lectin lectins mannose mechanism of action midgut Plant Lectins - antagonists & inhibitors Plant Lectins - metabolism Plant Lectins - pharmacology plant-insect interaction protein binding proteolysis resistance to digestion Sapindaceae Sapindaceae - chemistry seeds Seeds - chemistry storage insects Survival Analysis Talisia esculenta toxicology |
| title | Mechanisms of the insecticidal action of TEL (Talisia esculenta lectin) against Callosobruchus maculatus (Coleoptera: Bruchidae) |
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