Protective effect of LR-90 on articular cartilage in rabbit model of osteoarthritis
To investigate the protective effect of LR-90 on articular cartilage in rabbit model of osteoarthritis. The cultured rabbits chondrocytes were assigned to be treated with IL-1β (10ng/ml) or IL-1β (10ng/ml)+LR-90 (50 mg/L). The mRNA expression of MMP-13, ADAMTS-5, aggrecan and collagen II in chondroc...
Gespeichert in:
| Veröffentlicht in: | Zhejiang da xue xue bao. Journal of Zhejiang University. Medical sciences. Yi xue ban 2016-03, Vol.45 (2), p.187-194 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | chi |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 194 |
|---|---|
| container_issue | 2 |
| container_start_page | 187 |
| container_title | Zhejiang da xue xue bao. Journal of Zhejiang University. Medical sciences. Yi xue ban |
| container_volume | 45 |
| creator | Huang, Kai Zhang, Jianfang Wan, Junming Lu, Jianwei Gao, Yang |
| description | To investigate the protective effect of LR-90 on articular cartilage in rabbit model of osteoarthritis.
The cultured rabbits chondrocytes were assigned to be treated with IL-1β (10ng/ml) or IL-1β (10ng/ml)+LR-90 (50 mg/L). The mRNA expression of MMP-13, ADAMTS-5, aggrecan and collagen II in chondrocytes were assessed by real-time quantitative reverse transcription polymerase chain reaction (RT-PCR). Twenty male New Zealand white rabbits underwent bilateral anterior cruciate ligament transection (ACLT) to establish a animal model of osteoarthritis. Four weeks after model established, on the basis of randomization one knee of each rabbit was treated with 50 mg/L LR-90 in normal saline (NS) (experimental group) and the other knee was treated with same volume of NS (control group), 1/week × 5. Nine weeks after ACLT all rabbits were sacrificed and the knee joints were evaluated by gross morphology and histology. The mRNA expression of IL-1β, MMP-13, ADAMTS-5, aggrecan and collagen Ⅱ in articular cartilage was anal |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_proquest_miscellaneous_1795874617</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1795874617</sourcerecordid><originalsourceid>FETCH-LOGICAL-p126t-febce0446af43624324f6953ae1fd406b6eb74a1f9e5d0d6cf9aad2c207431b33</originalsourceid><addsrcrecordid>eNo1kEtLxDAYRbNQnGGcvyBZuink1WSylMEXFBQf65LHFw20zZikgv_eDo7cxT2Lw13cM7SmhOwazTRboW0p0ZKWcrWEXKAVU0xxrfkavT7nVMHV-A0YQlgIp4C7l0YTnCZsco1uHkzG7oiD-QAcJ5yNtbHiMXkYjn4qFdIifOZYY7lE58EMBban3qD3u9u3_UPTPd0_7m-65kCZrE0A64AIIU0QXDLBmQhSt9wADV4QaSVYJQwNGlpPvHRBG-OZY0QJTi3nG3T9t3vI6WuGUvsxFgfDYCZIc-mp0u1OCUnVol6d1NmO4PtDjqPJP_3_EfwXWHZZ4A</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1795874617</pqid></control><display><type>article</type><title>Protective effect of LR-90 on articular cartilage in rabbit model of osteoarthritis</title><source>MEDLINE</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central</source><creator>Huang, Kai ; Zhang, Jianfang ; Wan, Junming ; Lu, Jianwei ; Gao, Yang</creator><creatorcontrib>Huang, Kai ; Zhang, Jianfang ; Wan, Junming ; Lu, Jianwei ; Gao, Yang</creatorcontrib><description>To investigate the protective effect of LR-90 on articular cartilage in rabbit model of osteoarthritis.
The cultured rabbits chondrocytes were assigned to be treated with IL-1β (10ng/ml) or IL-1β (10ng/ml)+LR-90 (50 mg/L). The mRNA expression of MMP-13, ADAMTS-5, aggrecan and collagen II in chondrocytes were assessed by real-time quantitative reverse transcription polymerase chain reaction (RT-PCR). Twenty male New Zealand white rabbits underwent bilateral anterior cruciate ligament transection (ACLT) to establish a animal model of osteoarthritis. Four weeks after model established, on the basis of randomization one knee of each rabbit was treated with 50 mg/L LR-90 in normal saline (NS) (experimental group) and the other knee was treated with same volume of NS (control group), 1/week × 5. Nine weeks after ACLT all rabbits were sacrificed and the knee joints were evaluated by gross morphology and histology. The mRNA expression of IL-1β, MMP-13, ADAMTS-5, aggrecan and collagen Ⅱ in articular cartilage was anal</description><identifier>ISSN: 1008-9292</identifier><identifier>PMID: 27273993</identifier><language>chi</language><publisher>China</publisher><subject>ADAM Proteins - metabolism ; Aggrecans - metabolism ; Animals ; Anterior Cruciate Ligament - surgery ; Butyrates - pharmacology ; Cartilage, Articular - metabolism ; Cartilage, Articular - pathology ; Cells, Cultured ; Chondrocytes - metabolism ; Collagen Type II - metabolism ; Disease Models, Animal ; Injections, Intra-Articular ; Interleukin-1beta - pharmacology ; Male ; Matrix Metalloproteinase 13 - metabolism ; Osteoarthritis - drug therapy ; Rabbits</subject><ispartof>Zhejiang da xue xue bao. Journal of Zhejiang University. Medical sciences. Yi xue ban, 2016-03, Vol.45 (2), p.187-194</ispartof><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27273993$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Huang, Kai</creatorcontrib><creatorcontrib>Zhang, Jianfang</creatorcontrib><creatorcontrib>Wan, Junming</creatorcontrib><creatorcontrib>Lu, Jianwei</creatorcontrib><creatorcontrib>Gao, Yang</creatorcontrib><title>Protective effect of LR-90 on articular cartilage in rabbit model of osteoarthritis</title><title>Zhejiang da xue xue bao. Journal of Zhejiang University. Medical sciences. Yi xue ban</title><addtitle>Zhejiang Da Xue Xue Bao Yi Xue Ban</addtitle><description>To investigate the protective effect of LR-90 on articular cartilage in rabbit model of osteoarthritis.
The cultured rabbits chondrocytes were assigned to be treated with IL-1β (10ng/ml) or IL-1β (10ng/ml)+LR-90 (50 mg/L). The mRNA expression of MMP-13, ADAMTS-5, aggrecan and collagen II in chondrocytes were assessed by real-time quantitative reverse transcription polymerase chain reaction (RT-PCR). Twenty male New Zealand white rabbits underwent bilateral anterior cruciate ligament transection (ACLT) to establish a animal model of osteoarthritis. Four weeks after model established, on the basis of randomization one knee of each rabbit was treated with 50 mg/L LR-90 in normal saline (NS) (experimental group) and the other knee was treated with same volume of NS (control group), 1/week × 5. Nine weeks after ACLT all rabbits were sacrificed and the knee joints were evaluated by gross morphology and histology. The mRNA expression of IL-1β, MMP-13, ADAMTS-5, aggrecan and collagen Ⅱ in articular cartilage was anal</description><subject>ADAM Proteins - metabolism</subject><subject>Aggrecans - metabolism</subject><subject>Animals</subject><subject>Anterior Cruciate Ligament - surgery</subject><subject>Butyrates - pharmacology</subject><subject>Cartilage, Articular - metabolism</subject><subject>Cartilage, Articular - pathology</subject><subject>Cells, Cultured</subject><subject>Chondrocytes - metabolism</subject><subject>Collagen Type II - metabolism</subject><subject>Disease Models, Animal</subject><subject>Injections, Intra-Articular</subject><subject>Interleukin-1beta - pharmacology</subject><subject>Male</subject><subject>Matrix Metalloproteinase 13 - metabolism</subject><subject>Osteoarthritis - drug therapy</subject><subject>Rabbits</subject><issn>1008-9292</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo1kEtLxDAYRbNQnGGcvyBZuink1WSylMEXFBQf65LHFw20zZikgv_eDo7cxT2Lw13cM7SmhOwazTRboW0p0ZKWcrWEXKAVU0xxrfkavT7nVMHV-A0YQlgIp4C7l0YTnCZsco1uHkzG7oiD-QAcJ5yNtbHiMXkYjn4qFdIifOZYY7lE58EMBban3qD3u9u3_UPTPd0_7m-65kCZrE0A64AIIU0QXDLBmQhSt9wADV4QaSVYJQwNGlpPvHRBG-OZY0QJTi3nG3T9t3vI6WuGUvsxFgfDYCZIc-mp0u1OCUnVol6d1NmO4PtDjqPJP_3_EfwXWHZZ4A</recordid><startdate>201603</startdate><enddate>201603</enddate><creator>Huang, Kai</creator><creator>Zhang, Jianfang</creator><creator>Wan, Junming</creator><creator>Lu, Jianwei</creator><creator>Gao, Yang</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>201603</creationdate><title>Protective effect of LR-90 on articular cartilage in rabbit model of osteoarthritis</title><author>Huang, Kai ; Zhang, Jianfang ; Wan, Junming ; Lu, Jianwei ; Gao, Yang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p126t-febce0446af43624324f6953ae1fd406b6eb74a1f9e5d0d6cf9aad2c207431b33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>chi</language><creationdate>2016</creationdate><topic>ADAM Proteins - metabolism</topic><topic>Aggrecans - metabolism</topic><topic>Animals</topic><topic>Anterior Cruciate Ligament - surgery</topic><topic>Butyrates - pharmacology</topic><topic>Cartilage, Articular - metabolism</topic><topic>Cartilage, Articular - pathology</topic><topic>Cells, Cultured</topic><topic>Chondrocytes - metabolism</topic><topic>Collagen Type II - metabolism</topic><topic>Disease Models, Animal</topic><topic>Injections, Intra-Articular</topic><topic>Interleukin-1beta - pharmacology</topic><topic>Male</topic><topic>Matrix Metalloproteinase 13 - metabolism</topic><topic>Osteoarthritis - drug therapy</topic><topic>Rabbits</topic><toplevel>online_resources</toplevel><creatorcontrib>Huang, Kai</creatorcontrib><creatorcontrib>Zhang, Jianfang</creatorcontrib><creatorcontrib>Wan, Junming</creatorcontrib><creatorcontrib>Lu, Jianwei</creatorcontrib><creatorcontrib>Gao, Yang</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Zhejiang da xue xue bao. Journal of Zhejiang University. Medical sciences. Yi xue ban</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Huang, Kai</au><au>Zhang, Jianfang</au><au>Wan, Junming</au><au>Lu, Jianwei</au><au>Gao, Yang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Protective effect of LR-90 on articular cartilage in rabbit model of osteoarthritis</atitle><jtitle>Zhejiang da xue xue bao. Journal of Zhejiang University. Medical sciences. Yi xue ban</jtitle><addtitle>Zhejiang Da Xue Xue Bao Yi Xue Ban</addtitle><date>2016-03</date><risdate>2016</risdate><volume>45</volume><issue>2</issue><spage>187</spage><epage>194</epage><pages>187-194</pages><issn>1008-9292</issn><abstract>To investigate the protective effect of LR-90 on articular cartilage in rabbit model of osteoarthritis.
The cultured rabbits chondrocytes were assigned to be treated with IL-1β (10ng/ml) or IL-1β (10ng/ml)+LR-90 (50 mg/L). The mRNA expression of MMP-13, ADAMTS-5, aggrecan and collagen II in chondrocytes were assessed by real-time quantitative reverse transcription polymerase chain reaction (RT-PCR). Twenty male New Zealand white rabbits underwent bilateral anterior cruciate ligament transection (ACLT) to establish a animal model of osteoarthritis. Four weeks after model established, on the basis of randomization one knee of each rabbit was treated with 50 mg/L LR-90 in normal saline (NS) (experimental group) and the other knee was treated with same volume of NS (control group), 1/week × 5. Nine weeks after ACLT all rabbits were sacrificed and the knee joints were evaluated by gross morphology and histology. The mRNA expression of IL-1β, MMP-13, ADAMTS-5, aggrecan and collagen Ⅱ in articular cartilage was anal</abstract><cop>China</cop><pmid>27273993</pmid><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1008-9292 |
| ispartof | Zhejiang da xue xue bao. Journal of Zhejiang University. Medical sciences. Yi xue ban, 2016-03, Vol.45 (2), p.187-194 |
| issn | 1008-9292 |
| language | chi |
| recordid | cdi_proquest_miscellaneous_1795874617 |
| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central |
| subjects | ADAM Proteins - metabolism Aggrecans - metabolism Animals Anterior Cruciate Ligament - surgery Butyrates - pharmacology Cartilage, Articular - metabolism Cartilage, Articular - pathology Cells, Cultured Chondrocytes - metabolism Collagen Type II - metabolism Disease Models, Animal Injections, Intra-Articular Interleukin-1beta - pharmacology Male Matrix Metalloproteinase 13 - metabolism Osteoarthritis - drug therapy Rabbits |
| title | Protective effect of LR-90 on articular cartilage in rabbit model of osteoarthritis |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-04T15%3A16%3A49IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Protective%20effect%20of%20LR-90%20on%20articular%20cartilage%20in%20rabbit%20model%20of%20osteoarthritis&rft.jtitle=Zhejiang%20da%20xue%20xue%20bao.%20Journal%20of%20Zhejiang%20University.%20Medical%20sciences.%20Yi%20xue%20ban&rft.au=Huang,%20Kai&rft.date=2016-03&rft.volume=45&rft.issue=2&rft.spage=187&rft.epage=194&rft.pages=187-194&rft.issn=1008-9292&rft_id=info:doi/&rft_dat=%3Cproquest_pubme%3E1795874617%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1795874617&rft_id=info:pmid/27273993&rfr_iscdi=true |