Multidrug resistance and tumor-initiating capacity of oral cancer stem cells
Recent studies in several tumors showed that presence of cancer stem like side population (SP) cells are responsible for chemotherapeutic drugs resistance and tumor relapse. In our present study, we have analyzed the role of SP cells in oral squamous cell carcinoma cell (OSCC) line OSCC-77. The oral...
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| Veröffentlicht in: | Journal of B.U. ON. 2016-03, Vol.21 (2), p.461-465 |
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| creator | Shang, Hong-Guo Yu, Hong-Ling Ma, Xiao-Ni Xu, Xin |
| description | Recent studies in several tumors showed that presence of cancer stem like side population (SP) cells are responsible for chemotherapeutic drugs resistance and tumor relapse. In our present study, we have analyzed the role of SP cells in oral squamous cell carcinoma cell (OSCC) line OSCC-77.
The oral cancer cell line OSCC-77 was analyzed for the presence of SP cells by FACS using Hoechst 33342 dye exclusion method. Further the FACS-sorted SP and non-SP cells were subjected to drug resistance and sphere formation assays.
We identified that the presence of SP cells in OSCC-77 cell line was 3.4%, which was reduced to 0.6% in the presence of verapamil, an inhibitor of ABC transporter. Furthermore, we showed that these SP cells were highly drug-resistant, had increased survival and were highly potent for self-renewal. Also, the clone formation efficiency of SP cells was significantly higher compared to non-SP cells (p |
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The oral cancer cell line OSCC-77 was analyzed for the presence of SP cells by FACS using Hoechst 33342 dye exclusion method. Further the FACS-sorted SP and non-SP cells were subjected to drug resistance and sphere formation assays.
We identified that the presence of SP cells in OSCC-77 cell line was 3.4%, which was reduced to 0.6% in the presence of verapamil, an inhibitor of ABC transporter. Furthermore, we showed that these SP cells were highly drug-resistant, had increased survival and were highly potent for self-renewal. Also, the clone formation efficiency of SP cells was significantly higher compared to non-SP cells (p<0.01).
Our data suggest that cancer stem-like SP cells of OSCC-77 cell line contribute to multidrug resistance and are highly involved in tumor relapse. However, further characterization of SP cells at gene expression level and their signaling pathways might provide new insights into the development of novel anticancer drugs.</description><identifier>ISSN: 1107-0625</identifier><identifier>PMID: 27273959</identifier><language>eng</language><publisher>Greece</publisher><subject>Antineoplastic Agents - metabolism ; Antineoplastic Agents - pharmacology ; ATP-Binding Cassette Transporters - antagonists & inhibitors ; ATP-Binding Cassette Transporters - metabolism ; Cell Line, Tumor ; Cell Self Renewal - drug effects ; Cell Survival - drug effects ; Drug Resistance, Multiple ; Drug Resistance, Neoplasm ; Humans ; Mouth Neoplasms - drug therapy ; Mouth Neoplasms - metabolism ; Mouth Neoplasms - pathology ; Neoplastic Stem Cells - drug effects ; Neoplastic Stem Cells - metabolism ; Neoplastic Stem Cells - pathology ; Side-Population Cells - drug effects ; Side-Population Cells - metabolism ; Side-Population Cells - pathology ; Verapamil - pharmacology</subject><ispartof>Journal of B.U. ON., 2016-03, Vol.21 (2), p.461-465</ispartof><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27273959$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shang, Hong-Guo</creatorcontrib><creatorcontrib>Yu, Hong-Ling</creatorcontrib><creatorcontrib>Ma, Xiao-Ni</creatorcontrib><creatorcontrib>Xu, Xin</creatorcontrib><title>Multidrug resistance and tumor-initiating capacity of oral cancer stem cells</title><title>Journal of B.U. ON.</title><addtitle>J BUON</addtitle><description>Recent studies in several tumors showed that presence of cancer stem like side population (SP) cells are responsible for chemotherapeutic drugs resistance and tumor relapse. In our present study, we have analyzed the role of SP cells in oral squamous cell carcinoma cell (OSCC) line OSCC-77.
The oral cancer cell line OSCC-77 was analyzed for the presence of SP cells by FACS using Hoechst 33342 dye exclusion method. Further the FACS-sorted SP and non-SP cells were subjected to drug resistance and sphere formation assays.
We identified that the presence of SP cells in OSCC-77 cell line was 3.4%, which was reduced to 0.6% in the presence of verapamil, an inhibitor of ABC transporter. Furthermore, we showed that these SP cells were highly drug-resistant, had increased survival and were highly potent for self-renewal. Also, the clone formation efficiency of SP cells was significantly higher compared to non-SP cells (p<0.01).
Our data suggest that cancer stem-like SP cells of OSCC-77 cell line contribute to multidrug resistance and are highly involved in tumor relapse. However, further characterization of SP cells at gene expression level and their signaling pathways might provide new insights into the development of novel anticancer drugs.</description><subject>Antineoplastic Agents - metabolism</subject><subject>Antineoplastic Agents - pharmacology</subject><subject>ATP-Binding Cassette Transporters - antagonists & inhibitors</subject><subject>ATP-Binding Cassette Transporters - metabolism</subject><subject>Cell Line, Tumor</subject><subject>Cell Self Renewal - drug effects</subject><subject>Cell Survival - drug effects</subject><subject>Drug Resistance, Multiple</subject><subject>Drug Resistance, Neoplasm</subject><subject>Humans</subject><subject>Mouth Neoplasms - drug therapy</subject><subject>Mouth Neoplasms - metabolism</subject><subject>Mouth Neoplasms - pathology</subject><subject>Neoplastic Stem Cells - drug effects</subject><subject>Neoplastic Stem Cells - metabolism</subject><subject>Neoplastic Stem Cells - pathology</subject><subject>Side-Population Cells - drug effects</subject><subject>Side-Population Cells - metabolism</subject><subject>Side-Population Cells - pathology</subject><subject>Verapamil - pharmacology</subject><issn>1107-0625</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo1kElLBDEUhHNQnGGcvyA5emnI0i_dOcrgBiNe9Ny8TtJDpDezHObfG3Gsy4Oqj0dRV2TLOWsqpgRsyD7GL1akGFe6vSEb0YhGatBbcnzLY_I25BMNLvqYcDaO4mxpytMSKj_75DH5-UQNrmh8OtNloEvAsRiFDTQmN1HjxjHekusBx-j2l7sjn0-PH4eX6vj-_Hp4OFar4DxV1imrDPYN9CC4VDgAU0a31kDPFcDQg5K6tsUohRUDgXqwNdoS1EIyuSP3f3_XsHxnF1M3-fjbAGe35NjxRkOroGVQ0LsLmvvJ2W4NfsJw7v4XkD8bWleo</recordid><startdate>201603</startdate><enddate>201603</enddate><creator>Shang, Hong-Guo</creator><creator>Yu, Hong-Ling</creator><creator>Ma, Xiao-Ni</creator><creator>Xu, Xin</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>201603</creationdate><title>Multidrug resistance and tumor-initiating capacity of oral cancer stem cells</title><author>Shang, Hong-Guo ; Yu, Hong-Ling ; Ma, Xiao-Ni ; Xu, Xin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p211t-de6d6cab75b52136af506c98dc5b1655fb56394d8dc0166052a9fd4adfb542303</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Antineoplastic Agents - metabolism</topic><topic>Antineoplastic Agents - pharmacology</topic><topic>ATP-Binding Cassette Transporters - antagonists & inhibitors</topic><topic>ATP-Binding Cassette Transporters - metabolism</topic><topic>Cell Line, Tumor</topic><topic>Cell Self Renewal - drug effects</topic><topic>Cell Survival - drug effects</topic><topic>Drug Resistance, Multiple</topic><topic>Drug Resistance, Neoplasm</topic><topic>Humans</topic><topic>Mouth Neoplasms - drug therapy</topic><topic>Mouth Neoplasms - metabolism</topic><topic>Mouth Neoplasms - pathology</topic><topic>Neoplastic Stem Cells - drug effects</topic><topic>Neoplastic Stem Cells - metabolism</topic><topic>Neoplastic Stem Cells - pathology</topic><topic>Side-Population Cells - drug effects</topic><topic>Side-Population Cells - metabolism</topic><topic>Side-Population Cells - pathology</topic><topic>Verapamil - pharmacology</topic><toplevel>online_resources</toplevel><creatorcontrib>Shang, Hong-Guo</creatorcontrib><creatorcontrib>Yu, Hong-Ling</creatorcontrib><creatorcontrib>Ma, Xiao-Ni</creatorcontrib><creatorcontrib>Xu, Xin</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of B.U. ON.</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shang, Hong-Guo</au><au>Yu, Hong-Ling</au><au>Ma, Xiao-Ni</au><au>Xu, Xin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Multidrug resistance and tumor-initiating capacity of oral cancer stem cells</atitle><jtitle>Journal of B.U. ON.</jtitle><addtitle>J BUON</addtitle><date>2016-03</date><risdate>2016</risdate><volume>21</volume><issue>2</issue><spage>461</spage><epage>465</epage><pages>461-465</pages><issn>1107-0625</issn><abstract>Recent studies in several tumors showed that presence of cancer stem like side population (SP) cells are responsible for chemotherapeutic drugs resistance and tumor relapse. In our present study, we have analyzed the role of SP cells in oral squamous cell carcinoma cell (OSCC) line OSCC-77.
The oral cancer cell line OSCC-77 was analyzed for the presence of SP cells by FACS using Hoechst 33342 dye exclusion method. Further the FACS-sorted SP and non-SP cells were subjected to drug resistance and sphere formation assays.
We identified that the presence of SP cells in OSCC-77 cell line was 3.4%, which was reduced to 0.6% in the presence of verapamil, an inhibitor of ABC transporter. Furthermore, we showed that these SP cells were highly drug-resistant, had increased survival and were highly potent for self-renewal. Also, the clone formation efficiency of SP cells was significantly higher compared to non-SP cells (p<0.01).
Our data suggest that cancer stem-like SP cells of OSCC-77 cell line contribute to multidrug resistance and are highly involved in tumor relapse. However, further characterization of SP cells at gene expression level and their signaling pathways might provide new insights into the development of novel anticancer drugs.</abstract><cop>Greece</cop><pmid>27273959</pmid><tpages>5</tpages></addata></record> |
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| subjects | Antineoplastic Agents - metabolism Antineoplastic Agents - pharmacology ATP-Binding Cassette Transporters - antagonists & inhibitors ATP-Binding Cassette Transporters - metabolism Cell Line, Tumor Cell Self Renewal - drug effects Cell Survival - drug effects Drug Resistance, Multiple Drug Resistance, Neoplasm Humans Mouth Neoplasms - drug therapy Mouth Neoplasms - metabolism Mouth Neoplasms - pathology Neoplastic Stem Cells - drug effects Neoplastic Stem Cells - metabolism Neoplastic Stem Cells - pathology Side-Population Cells - drug effects Side-Population Cells - metabolism Side-Population Cells - pathology Verapamil - pharmacology |
| title | Multidrug resistance and tumor-initiating capacity of oral cancer stem cells |
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