Dual-frequency ultrasound activation of nanomicellar doxorubicin in targeted tumor chemotherapy
Introduction This study investigated the therapeutic effect of dual-frequency sonication (3 MHz and 28 kHz) at low intensity levels in combination with micellar doxorubicin in the treatment of a tumor model of spontaneous breast adenocarcinoma in Balb/c mice. Methods We used sonication frequencies 2...
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| Veröffentlicht in: | Journal of medical ultrasonics (2001) 2014-04, Vol.41 (2), p.139-150 |
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| creator | Hasanzadeh, Hadi Mokhtari-Dizaji, Manijhe Zahra Bathaie, S. Hassan, Zuhair M. Shahbazfar, Amir Ali |
| description | Introduction
This study investigated the therapeutic effect of dual-frequency sonication (3 MHz and 28 kHz) at low intensity levels in combination with micellar doxorubicin in the treatment of a tumor model of spontaneous breast adenocarcinoma in Balb/c mice.
Methods
We used sonication frequencies 28 kHz and 3 MHz and their dual combinations in the progressive wave mode to enhance acoustic cavitation. Then, the antitumor effect of the simultaneous dual-frequency ultrasound (28 kHz and 3 MHz) at low intensity levels in combination with doxorubicin and micellar doxorubicin injection was investigated in a spontaneous model of breast adenocarcinoma in Balb/c mice. Sixty-three tumor-bearing mice were randomly divided into seven groups: control, sham, sonication with dual frequency, doxorubicin without sonication, doxorubicin with dual-frequency sonication, micellar doxorubicin without sonication, and micellar doxorubicin with dual-frequency sonication. The tumor volume change relative to the initial volume, tumor growth inhibition ratio, the required times for each tumor to reach two (
T
2
) and five (
T
5
) times its initial volume, and survival period were the tumor growth delay parameters which were calculated and recorded at various times after treatment.
Results
The results of the combination of frequencies 28 kHz (0.04 W/cm
2
) and 3 MHz (2.00 W/cm
2
) showed remarkable enhancement of the cavitation activity compared with single-frequency sonication (
P
|
| doi_str_mv | 10.1007/s10396-013-0484-x |
| format | Article |
| fullrecord | <record><control><sourceid>gale_proqu</sourceid><recordid>TN_cdi_proquest_miscellaneous_1795863253</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A714891538</galeid><sourcerecordid>A714891538</sourcerecordid><originalsourceid>FETCH-LOGICAL-c463t-9d4c592e4963faa67fbcf149fa338fcbc6c9f53c7cabe7ab65587e532a0407a73</originalsourceid><addsrcrecordid>eNp1kcluFDEQhi0EIgs8ABfUEhcuHbwvxyiBgBSJC5wtt9ueOOq2By9o5u3xMGEVyJZctr-_VFU_AC8QvEAQijcFQaL4CBEZIZV03D0Cp4j3G8aMPu4xoXykDJMTcFbKPYSUUIifghMssBCCs1Ogr5tZRp_dl-ai3Q9tqdmU1OI8GFvDV1NDikPyQzQxrcG6ZTF5mNMu5TYFG-LQdzV546qbh9rWlAd759ZU71w22_0z8MSbpbjnD-c5-Pzu7aer9-Ptx5sPV5e3o6Wc1FHN1DKFHVWceGO48JP1iCpvCJHeTpZb5RmxwprJCTNxxqRwjGADKRRGkHPw-ph3m1NvpVS9hvK92uhSKxoJxSQnmJGOvvoLvU8tx16dxgpJJAWVv1Ebszgdok99MPaQVF8KRKVCjMhOXfyD6mt2fVgpOh_6-x8CdBTYnErJzuttDqvJe42gPpiqj6bqbqo-mKp3XfPyoeA2rW7-qfjhYgfwESj9K25c_tXR_7N-A_P0rZw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2918187483</pqid></control><display><type>article</type><title>Dual-frequency ultrasound activation of nanomicellar doxorubicin in targeted tumor chemotherapy</title><source>Springer Nature</source><source>ProQuest Central (Alumni)</source><source>ProQuest Central</source><creator>Hasanzadeh, Hadi ; Mokhtari-Dizaji, Manijhe ; Zahra Bathaie, S. ; Hassan, Zuhair M. ; Shahbazfar, Amir Ali</creator><creatorcontrib>Hasanzadeh, Hadi ; Mokhtari-Dizaji, Manijhe ; Zahra Bathaie, S. ; Hassan, Zuhair M. ; Shahbazfar, Amir Ali</creatorcontrib><description>Introduction
This study investigated the therapeutic effect of dual-frequency sonication (3 MHz and 28 kHz) at low intensity levels in combination with micellar doxorubicin in the treatment of a tumor model of spontaneous breast adenocarcinoma in Balb/c mice.
Methods
We used sonication frequencies 28 kHz and 3 MHz and their dual combinations in the progressive wave mode to enhance acoustic cavitation. Then, the antitumor effect of the simultaneous dual-frequency ultrasound (28 kHz and 3 MHz) at low intensity levels in combination with doxorubicin and micellar doxorubicin injection was investigated in a spontaneous model of breast adenocarcinoma in Balb/c mice. Sixty-three tumor-bearing mice were randomly divided into seven groups: control, sham, sonication with dual frequency, doxorubicin without sonication, doxorubicin with dual-frequency sonication, micellar doxorubicin without sonication, and micellar doxorubicin with dual-frequency sonication. The tumor volume change relative to the initial volume, tumor growth inhibition ratio, the required times for each tumor to reach two (
T
2
) and five (
T
5
) times its initial volume, and survival period were the tumor growth delay parameters which were calculated and recorded at various times after treatment.
Results
The results of the combination of frequencies 28 kHz (0.04 W/cm
2
) and 3 MHz (2.00 W/cm
2
) showed remarkable enhancement of the cavitation activity compared with single-frequency sonication (
P
< 0.05). The micellar doxorubicin injection with sonication group showed a significant difference in the relative volume percent parameter compared with the other groups (
P
< 0.05). Additionally, the
T
2
and
T
5
times in the micellar doxorubicin with sonication group were significantly higher than in the other groups (
P
< 0.05). Also, the survival period of the mice in the micellar doxorubicin with sonication group was significantly longer than in the other groups (
P
< 0.05). These findings were verified histopathologically.
Conclusion
This study shows that simultaneous combined dual-frequency ultrasound sonication in continuous mode is effective in producing cavitation activity at low intensity. We conclude that dual-frequency sonication with micellar doxorubicin injection extends survival in a murine breast adenocarcinoma model.</description><identifier>ISSN: 1346-4523</identifier><identifier>EISSN: 1613-2254</identifier><identifier>DOI: 10.1007/s10396-013-0484-x</identifier><identifier>PMID: 27277765</identifier><language>eng</language><publisher>Tokyo: Springer Japan</publisher><subject>Acoustics ; Analysis ; Anticancer properties ; Breast ; Breast cancer ; Cancer ; Cavitation ; Chemotherapy ; Doxorubicin ; Fourier transforms ; Health aspects ; Imaging ; Mathematical models ; Medicine ; Medicine & Public Health ; Nitrogen ; Original Article ; Parameters ; Radiation ; Radiology ; Spectrum analysis ; Survival ; Tumors ; Ultrasonic imaging ; Ultrasound</subject><ispartof>Journal of medical ultrasonics (2001), 2014-04, Vol.41 (2), p.139-150</ispartof><rights>The Japan Society of Ultrasonics in Medicine 2013</rights><rights>COPYRIGHT 2014 Springer</rights><rights>The Japan Society of Ultrasonics in Medicine 2013.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c463t-9d4c592e4963faa67fbcf149fa338fcbc6c9f53c7cabe7ab65587e532a0407a73</citedby><cites>FETCH-LOGICAL-c463t-9d4c592e4963faa67fbcf149fa338fcbc6c9f53c7cabe7ab65587e532a0407a73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10396-013-0484-x$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/2918187483?pq-origsite=primo$$EHTML$$P50$$Gproquest$$H</linktohtml><link.rule.ids>314,780,784,21388,21389,27924,27925,33530,33531,33744,33745,41488,42557,43659,43805,51319,64385,64387,64389,72469</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27277765$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hasanzadeh, Hadi</creatorcontrib><creatorcontrib>Mokhtari-Dizaji, Manijhe</creatorcontrib><creatorcontrib>Zahra Bathaie, S.</creatorcontrib><creatorcontrib>Hassan, Zuhair M.</creatorcontrib><creatorcontrib>Shahbazfar, Amir Ali</creatorcontrib><title>Dual-frequency ultrasound activation of nanomicellar doxorubicin in targeted tumor chemotherapy</title><title>Journal of medical ultrasonics (2001)</title><addtitle>J Med Ultrasonics</addtitle><addtitle>J Med Ultrason (2001)</addtitle><description>Introduction
This study investigated the therapeutic effect of dual-frequency sonication (3 MHz and 28 kHz) at low intensity levels in combination with micellar doxorubicin in the treatment of a tumor model of spontaneous breast adenocarcinoma in Balb/c mice.
Methods
We used sonication frequencies 28 kHz and 3 MHz and their dual combinations in the progressive wave mode to enhance acoustic cavitation. Then, the antitumor effect of the simultaneous dual-frequency ultrasound (28 kHz and 3 MHz) at low intensity levels in combination with doxorubicin and micellar doxorubicin injection was investigated in a spontaneous model of breast adenocarcinoma in Balb/c mice. Sixty-three tumor-bearing mice were randomly divided into seven groups: control, sham, sonication with dual frequency, doxorubicin without sonication, doxorubicin with dual-frequency sonication, micellar doxorubicin without sonication, and micellar doxorubicin with dual-frequency sonication. The tumor volume change relative to the initial volume, tumor growth inhibition ratio, the required times for each tumor to reach two (
T
2
) and five (
T
5
) times its initial volume, and survival period were the tumor growth delay parameters which were calculated and recorded at various times after treatment.
Results
The results of the combination of frequencies 28 kHz (0.04 W/cm
2
) and 3 MHz (2.00 W/cm
2
) showed remarkable enhancement of the cavitation activity compared with single-frequency sonication (
P
< 0.05). The micellar doxorubicin injection with sonication group showed a significant difference in the relative volume percent parameter compared with the other groups (
P
< 0.05). Additionally, the
T
2
and
T
5
times in the micellar doxorubicin with sonication group were significantly higher than in the other groups (
P
< 0.05). Also, the survival period of the mice in the micellar doxorubicin with sonication group was significantly longer than in the other groups (
P
< 0.05). These findings were verified histopathologically.
Conclusion
This study shows that simultaneous combined dual-frequency ultrasound sonication in continuous mode is effective in producing cavitation activity at low intensity. We conclude that dual-frequency sonication with micellar doxorubicin injection extends survival in a murine breast adenocarcinoma model.</description><subject>Acoustics</subject><subject>Analysis</subject><subject>Anticancer properties</subject><subject>Breast</subject><subject>Breast cancer</subject><subject>Cancer</subject><subject>Cavitation</subject><subject>Chemotherapy</subject><subject>Doxorubicin</subject><subject>Fourier transforms</subject><subject>Health aspects</subject><subject>Imaging</subject><subject>Mathematical models</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Nitrogen</subject><subject>Original Article</subject><subject>Parameters</subject><subject>Radiation</subject><subject>Radiology</subject><subject>Spectrum analysis</subject><subject>Survival</subject><subject>Tumors</subject><subject>Ultrasonic imaging</subject><subject>Ultrasound</subject><issn>1346-4523</issn><issn>1613-2254</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><recordid>eNp1kcluFDEQhi0EIgs8ABfUEhcuHbwvxyiBgBSJC5wtt9ueOOq2By9o5u3xMGEVyJZctr-_VFU_AC8QvEAQijcFQaL4CBEZIZV03D0Cp4j3G8aMPu4xoXykDJMTcFbKPYSUUIifghMssBCCs1Ogr5tZRp_dl-ai3Q9tqdmU1OI8GFvDV1NDikPyQzQxrcG6ZTF5mNMu5TYFG-LQdzV546qbh9rWlAd759ZU71w22_0z8MSbpbjnD-c5-Pzu7aer9-Ptx5sPV5e3o6Wc1FHN1DKFHVWceGO48JP1iCpvCJHeTpZb5RmxwprJCTNxxqRwjGADKRRGkHPw-ph3m1NvpVS9hvK92uhSKxoJxSQnmJGOvvoLvU8tx16dxgpJJAWVv1Ebszgdok99MPaQVF8KRKVCjMhOXfyD6mt2fVgpOh_6-x8CdBTYnErJzuttDqvJe42gPpiqj6bqbqo-mKp3XfPyoeA2rW7-qfjhYgfwESj9K25c_tXR_7N-A_P0rZw</recordid><startdate>20140401</startdate><enddate>20140401</enddate><creator>Hasanzadeh, Hadi</creator><creator>Mokhtari-Dizaji, Manijhe</creator><creator>Zahra Bathaie, S.</creator><creator>Hassan, Zuhair M.</creator><creator>Shahbazfar, Amir Ali</creator><general>Springer Japan</general><general>Springer</general><general>Springer Nature B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7X7</scope><scope>7XB</scope><scope>8FE</scope><scope>8FG</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB0</scope><scope>M0S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7X8</scope></search><sort><creationdate>20140401</creationdate><title>Dual-frequency ultrasound activation of nanomicellar doxorubicin in targeted tumor chemotherapy</title><author>Hasanzadeh, Hadi ; Mokhtari-Dizaji, Manijhe ; Zahra Bathaie, S. ; Hassan, Zuhair M. ; Shahbazfar, Amir Ali</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c463t-9d4c592e4963faa67fbcf149fa338fcbc6c9f53c7cabe7ab65587e532a0407a73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Acoustics</topic><topic>Analysis</topic><topic>Anticancer properties</topic><topic>Breast</topic><topic>Breast cancer</topic><topic>Cancer</topic><topic>Cavitation</topic><topic>Chemotherapy</topic><topic>Doxorubicin</topic><topic>Fourier transforms</topic><topic>Health aspects</topic><topic>Imaging</topic><topic>Mathematical models</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Nitrogen</topic><topic>Original Article</topic><topic>Parameters</topic><topic>Radiation</topic><topic>Radiology</topic><topic>Spectrum analysis</topic><topic>Survival</topic><topic>Tumors</topic><topic>Ultrasonic imaging</topic><topic>Ultrasound</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hasanzadeh, Hadi</creatorcontrib><creatorcontrib>Mokhtari-Dizaji, Manijhe</creatorcontrib><creatorcontrib>Zahra Bathaie, S.</creatorcontrib><creatorcontrib>Hassan, Zuhair M.</creatorcontrib><creatorcontrib>Shahbazfar, Amir Ali</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Nursing & Allied Health Database</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Nursing & Allied Health Premium</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of medical ultrasonics (2001)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hasanzadeh, Hadi</au><au>Mokhtari-Dizaji, Manijhe</au><au>Zahra Bathaie, S.</au><au>Hassan, Zuhair M.</au><au>Shahbazfar, Amir Ali</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Dual-frequency ultrasound activation of nanomicellar doxorubicin in targeted tumor chemotherapy</atitle><jtitle>Journal of medical ultrasonics (2001)</jtitle><stitle>J Med Ultrasonics</stitle><addtitle>J Med Ultrason (2001)</addtitle><date>2014-04-01</date><risdate>2014</risdate><volume>41</volume><issue>2</issue><spage>139</spage><epage>150</epage><pages>139-150</pages><issn>1346-4523</issn><eissn>1613-2254</eissn><abstract>Introduction
This study investigated the therapeutic effect of dual-frequency sonication (3 MHz and 28 kHz) at low intensity levels in combination with micellar doxorubicin in the treatment of a tumor model of spontaneous breast adenocarcinoma in Balb/c mice.
Methods
We used sonication frequencies 28 kHz and 3 MHz and their dual combinations in the progressive wave mode to enhance acoustic cavitation. Then, the antitumor effect of the simultaneous dual-frequency ultrasound (28 kHz and 3 MHz) at low intensity levels in combination with doxorubicin and micellar doxorubicin injection was investigated in a spontaneous model of breast adenocarcinoma in Balb/c mice. Sixty-three tumor-bearing mice were randomly divided into seven groups: control, sham, sonication with dual frequency, doxorubicin without sonication, doxorubicin with dual-frequency sonication, micellar doxorubicin without sonication, and micellar doxorubicin with dual-frequency sonication. The tumor volume change relative to the initial volume, tumor growth inhibition ratio, the required times for each tumor to reach two (
T
2
) and five (
T
5
) times its initial volume, and survival period were the tumor growth delay parameters which were calculated and recorded at various times after treatment.
Results
The results of the combination of frequencies 28 kHz (0.04 W/cm
2
) and 3 MHz (2.00 W/cm
2
) showed remarkable enhancement of the cavitation activity compared with single-frequency sonication (
P
< 0.05). The micellar doxorubicin injection with sonication group showed a significant difference in the relative volume percent parameter compared with the other groups (
P
< 0.05). Additionally, the
T
2
and
T
5
times in the micellar doxorubicin with sonication group were significantly higher than in the other groups (
P
< 0.05). Also, the survival period of the mice in the micellar doxorubicin with sonication group was significantly longer than in the other groups (
P
< 0.05). These findings were verified histopathologically.
Conclusion
This study shows that simultaneous combined dual-frequency ultrasound sonication in continuous mode is effective in producing cavitation activity at low intensity. We conclude that dual-frequency sonication with micellar doxorubicin injection extends survival in a murine breast adenocarcinoma model.</abstract><cop>Tokyo</cop><pub>Springer Japan</pub><pmid>27277765</pmid><doi>10.1007/s10396-013-0484-x</doi><tpages>12</tpages></addata></record> |
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| source | Springer Nature; ProQuest Central (Alumni); ProQuest Central |
| subjects | Acoustics Analysis Anticancer properties Breast Breast cancer Cancer Cavitation Chemotherapy Doxorubicin Fourier transforms Health aspects Imaging Mathematical models Medicine Medicine & Public Health Nitrogen Original Article Parameters Radiation Radiology Spectrum analysis Survival Tumors Ultrasonic imaging Ultrasound |
| title | Dual-frequency ultrasound activation of nanomicellar doxorubicin in targeted tumor chemotherapy |
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