A polyphasic taxonomic approach in isolated strains of Cyanobacteria from thermal springs of Greece
[Display omitted] •Systematics of Cyanobacteria is in a state of flux.•Cyanobacteria from Greek thermal springs have been selected for cultivation and taxonomic study.•A polyphasic taxonomic approach was implemented to 17 strains of Cyanobacteria.•Among them 6 new taxa at the genus level have been p...
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| Veröffentlicht in: | Molecular phylogenetics and evolution 2016-05, Vol.98, p.147-160 |
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| container_title | Molecular phylogenetics and evolution |
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| creator | Bravakos, Panos Kotoulas, Georgios Skaraki, Katerina Pantazidou, Adriani Economou-Amilli, Athena |
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•Systematics of Cyanobacteria is in a state of flux.•Cyanobacteria from Greek thermal springs have been selected for cultivation and taxonomic study.•A polyphasic taxonomic approach was implemented to 17 strains of Cyanobacteria.•Among them 6 new taxa at the genus level have been proposed.
Strains of Cyanobacteria isolated from mats of 9 thermal springs of Greece have been studied for their taxonomic evaluation. A polyphasic taxonomic approach was employed which included: morphological observations by light microscopy and scanning electron microscopy, maximum parsimony, maximum likelihood and Bayesian analysis of 16S rDNA sequences, secondary structural comparisons of 16S–23S rRNA Internal Transcribed Spacer sequences, and finally environmental data. The 17 cyanobacterial isolates formed a diverse group that contained filamentous, coccoid and heterocytous strains. These included representatives of the polyphyletic genera of Synechococcus and Phormidium, and the orders Oscillatoriales, Spirulinales, Chroococcales and Nostocales. After analysis, at least 6 new taxa at the genus level provide new evidence in the taxonomy of Cyanobacteria and highlight the abundant diversity of thermal spring environments with many potential endemic species or ecotypes. |
| doi_str_mv | 10.1016/j.ympev.2016.02.009 |
| format | Article |
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•Systematics of Cyanobacteria is in a state of flux.•Cyanobacteria from Greek thermal springs have been selected for cultivation and taxonomic study.•A polyphasic taxonomic approach was implemented to 17 strains of Cyanobacteria.•Among them 6 new taxa at the genus level have been proposed.
Strains of Cyanobacteria isolated from mats of 9 thermal springs of Greece have been studied for their taxonomic evaluation. A polyphasic taxonomic approach was employed which included: morphological observations by light microscopy and scanning electron microscopy, maximum parsimony, maximum likelihood and Bayesian analysis of 16S rDNA sequences, secondary structural comparisons of 16S–23S rRNA Internal Transcribed Spacer sequences, and finally environmental data. The 17 cyanobacterial isolates formed a diverse group that contained filamentous, coccoid and heterocytous strains. These included representatives of the polyphyletic genera of Synechococcus and Phormidium, and the orders Oscillatoriales, Spirulinales, Chroococcales and Nostocales. After analysis, at least 6 new taxa at the genus level provide new evidence in the taxonomy of Cyanobacteria and highlight the abundant diversity of thermal spring environments with many potential endemic species or ecotypes.</description><identifier>ISSN: 1055-7903</identifier><identifier>EISSN: 1095-9513</identifier><identifier>DOI: 10.1016/j.ympev.2016.02.009</identifier><identifier>PMID: 26899923</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Bayes Theorem ; Chroococcales ; Cyanobacteria ; Cyanobacteria - classification ; Cyanobacteria - cytology ; Cyanobacteria - genetics ; Cyanobacteria - isolation & purification ; Cyanobacteria - ultrastructure ; DNA, Bacterial - genetics ; DNA, Ribosomal - genetics ; Greece ; Hot Springs - microbiology ; Likelihood Functions ; Microscopy ; Molecular phylogeny ; Morphology ; Nostocales ; Oscillatoriales ; Phormidium ; Phylogeny ; Polyphasic taxonomy ; RNA, Bacterial - genetics ; RNA, Ribosomal - genetics ; rRNA secondary structures ; Sequence Analysis, DNA ; Synechococcus ; Thermal springs</subject><ispartof>Molecular phylogenetics and evolution, 2016-05, Vol.98, p.147-160</ispartof><rights>2016 Elsevier Inc.</rights><rights>Copyright © 2016 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c392t-de9af1991c4c6ba2bbec353e74e27fab98014fd6e4353695aac93c3fee621a9c3</citedby><cites>FETCH-LOGICAL-c392t-de9af1991c4c6ba2bbec353e74e27fab98014fd6e4353695aac93c3fee621a9c3</cites><orcidid>0000-0002-8279-8655 ; 0000-0003-1989-8458</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.ympev.2016.02.009$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,778,782,3539,27907,27908,45978</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26899923$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bravakos, Panos</creatorcontrib><creatorcontrib>Kotoulas, Georgios</creatorcontrib><creatorcontrib>Skaraki, Katerina</creatorcontrib><creatorcontrib>Pantazidou, Adriani</creatorcontrib><creatorcontrib>Economou-Amilli, Athena</creatorcontrib><title>A polyphasic taxonomic approach in isolated strains of Cyanobacteria from thermal springs of Greece</title><title>Molecular phylogenetics and evolution</title><addtitle>Mol Phylogenet Evol</addtitle><description>[Display omitted]
•Systematics of Cyanobacteria is in a state of flux.•Cyanobacteria from Greek thermal springs have been selected for cultivation and taxonomic study.•A polyphasic taxonomic approach was implemented to 17 strains of Cyanobacteria.•Among them 6 new taxa at the genus level have been proposed.
Strains of Cyanobacteria isolated from mats of 9 thermal springs of Greece have been studied for their taxonomic evaluation. A polyphasic taxonomic approach was employed which included: morphological observations by light microscopy and scanning electron microscopy, maximum parsimony, maximum likelihood and Bayesian analysis of 16S rDNA sequences, secondary structural comparisons of 16S–23S rRNA Internal Transcribed Spacer sequences, and finally environmental data. The 17 cyanobacterial isolates formed a diverse group that contained filamentous, coccoid and heterocytous strains. These included representatives of the polyphyletic genera of Synechococcus and Phormidium, and the orders Oscillatoriales, Spirulinales, Chroococcales and Nostocales. After analysis, at least 6 new taxa at the genus level provide new evidence in the taxonomy of Cyanobacteria and highlight the abundant diversity of thermal spring environments with many potential endemic species or ecotypes.</description><subject>Bayes Theorem</subject><subject>Chroococcales</subject><subject>Cyanobacteria</subject><subject>Cyanobacteria - classification</subject><subject>Cyanobacteria - cytology</subject><subject>Cyanobacteria - genetics</subject><subject>Cyanobacteria - isolation & purification</subject><subject>Cyanobacteria - ultrastructure</subject><subject>DNA, Bacterial - genetics</subject><subject>DNA, Ribosomal - genetics</subject><subject>Greece</subject><subject>Hot Springs - microbiology</subject><subject>Likelihood Functions</subject><subject>Microscopy</subject><subject>Molecular phylogeny</subject><subject>Morphology</subject><subject>Nostocales</subject><subject>Oscillatoriales</subject><subject>Phormidium</subject><subject>Phylogeny</subject><subject>Polyphasic taxonomy</subject><subject>RNA, Bacterial - genetics</subject><subject>RNA, Ribosomal - genetics</subject><subject>rRNA secondary structures</subject><subject>Sequence Analysis, DNA</subject><subject>Synechococcus</subject><subject>Thermal springs</subject><issn>1055-7903</issn><issn>1095-9513</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkUtv1DAQxy1ERUvbT4CEfOSS4EecZA4cqlUplSpxoWdr4kxYr5I42NmK_fZ4u4Uj6mke-s3zz9gHKUopZP15Vx6mhZ5KlYNSqFIIeMMupABTgJH67dE3pmhA6HP2PqWdEFIaMO_YuapbAFD6grkbvoTxsGwxecdX_B3mMGUPlyUGdFvuZ-5TGHGlnqc1op8TDwPfHHAOHbqVokc-xDDxdUtxwpGnJfr55zN1F4kcXbGzAcdE1y_2kj1-vf2x-VY8fL-739w8FE6DWoueAAcJIF3l6g5V15HTRlNTkWoG7KAVshr6mqqcrcEgOtBOD0S1kghOX7JPp7559V97SqudfHI0jjhT2CcrG6iMMDW0r0CbtpGyVZBRfUJdDClFGmy-b8J4sFLYoxB2Z5-FsEchrFA2C5GrPr4M2HcT9f9q_n4-A19OAOWPPHmKNjlPs6PeR3Kr7YP_74A_OQSchQ</recordid><startdate>201605</startdate><enddate>201605</enddate><creator>Bravakos, Panos</creator><creator>Kotoulas, Georgios</creator><creator>Skaraki, Katerina</creator><creator>Pantazidou, Adriani</creator><creator>Economou-Amilli, Athena</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>M7N</scope><orcidid>https://orcid.org/0000-0002-8279-8655</orcidid><orcidid>https://orcid.org/0000-0003-1989-8458</orcidid></search><sort><creationdate>201605</creationdate><title>A polyphasic taxonomic approach in isolated strains of Cyanobacteria from thermal springs of Greece</title><author>Bravakos, Panos ; Kotoulas, Georgios ; Skaraki, Katerina ; Pantazidou, Adriani ; Economou-Amilli, Athena</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c392t-de9af1991c4c6ba2bbec353e74e27fab98014fd6e4353695aac93c3fee621a9c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Bayes Theorem</topic><topic>Chroococcales</topic><topic>Cyanobacteria</topic><topic>Cyanobacteria - classification</topic><topic>Cyanobacteria - cytology</topic><topic>Cyanobacteria - genetics</topic><topic>Cyanobacteria - isolation & purification</topic><topic>Cyanobacteria - ultrastructure</topic><topic>DNA, Bacterial - genetics</topic><topic>DNA, Ribosomal - genetics</topic><topic>Greece</topic><topic>Hot Springs - microbiology</topic><topic>Likelihood Functions</topic><topic>Microscopy</topic><topic>Molecular phylogeny</topic><topic>Morphology</topic><topic>Nostocales</topic><topic>Oscillatoriales</topic><topic>Phormidium</topic><topic>Phylogeny</topic><topic>Polyphasic taxonomy</topic><topic>RNA, Bacterial - genetics</topic><topic>RNA, Ribosomal - genetics</topic><topic>rRNA secondary structures</topic><topic>Sequence Analysis, DNA</topic><topic>Synechococcus</topic><topic>Thermal springs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bravakos, Panos</creatorcontrib><creatorcontrib>Kotoulas, Georgios</creatorcontrib><creatorcontrib>Skaraki, Katerina</creatorcontrib><creatorcontrib>Pantazidou, Adriani</creatorcontrib><creatorcontrib>Economou-Amilli, Athena</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><jtitle>Molecular phylogenetics and evolution</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bravakos, Panos</au><au>Kotoulas, Georgios</au><au>Skaraki, Katerina</au><au>Pantazidou, Adriani</au><au>Economou-Amilli, Athena</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A polyphasic taxonomic approach in isolated strains of Cyanobacteria from thermal springs of Greece</atitle><jtitle>Molecular phylogenetics and evolution</jtitle><addtitle>Mol Phylogenet Evol</addtitle><date>2016-05</date><risdate>2016</risdate><volume>98</volume><spage>147</spage><epage>160</epage><pages>147-160</pages><issn>1055-7903</issn><eissn>1095-9513</eissn><abstract>[Display omitted]
•Systematics of Cyanobacteria is in a state of flux.•Cyanobacteria from Greek thermal springs have been selected for cultivation and taxonomic study.•A polyphasic taxonomic approach was implemented to 17 strains of Cyanobacteria.•Among them 6 new taxa at the genus level have been proposed.
Strains of Cyanobacteria isolated from mats of 9 thermal springs of Greece have been studied for their taxonomic evaluation. A polyphasic taxonomic approach was employed which included: morphological observations by light microscopy and scanning electron microscopy, maximum parsimony, maximum likelihood and Bayesian analysis of 16S rDNA sequences, secondary structural comparisons of 16S–23S rRNA Internal Transcribed Spacer sequences, and finally environmental data. The 17 cyanobacterial isolates formed a diverse group that contained filamentous, coccoid and heterocytous strains. These included representatives of the polyphyletic genera of Synechococcus and Phormidium, and the orders Oscillatoriales, Spirulinales, Chroococcales and Nostocales. After analysis, at least 6 new taxa at the genus level provide new evidence in the taxonomy of Cyanobacteria and highlight the abundant diversity of thermal spring environments with many potential endemic species or ecotypes.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>26899923</pmid><doi>10.1016/j.ympev.2016.02.009</doi><tpages>14</tpages><orcidid>https://orcid.org/0000-0002-8279-8655</orcidid><orcidid>https://orcid.org/0000-0003-1989-8458</orcidid></addata></record> |
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| subjects | Bayes Theorem Chroococcales Cyanobacteria Cyanobacteria - classification Cyanobacteria - cytology Cyanobacteria - genetics Cyanobacteria - isolation & purification Cyanobacteria - ultrastructure DNA, Bacterial - genetics DNA, Ribosomal - genetics Greece Hot Springs - microbiology Likelihood Functions Microscopy Molecular phylogeny Morphology Nostocales Oscillatoriales Phormidium Phylogeny Polyphasic taxonomy RNA, Bacterial - genetics RNA, Ribosomal - genetics rRNA secondary structures Sequence Analysis, DNA Synechococcus Thermal springs |
| title | A polyphasic taxonomic approach in isolated strains of Cyanobacteria from thermal springs of Greece |
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