Heterologous live infectious bronchitis virus vaccination in day-old commercial broiler chicks: clinical signs, ciliary health, immune responses and protection against variant infectious bronchitis viruses
Groups of one-day-old broiler chicks were vaccinated via the oculo-nasal route with different live infectious bronchitis virus (IBV) vaccines: Massachusetts (Mass), 793B, D274 or Arkansas (Ark). Clinical signs and gross lesions were evaluated. Five chicks from each group were humanely killed at inte...
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| Veröffentlicht in: | Avian pathology 2016-03, Vol.45 (2), p.169-177 |
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| creator | Awad, Faez Hutton, Sally Forrester, Anne Baylis, Matthew Ganapathy, Kannan |
| description | Groups of one-day-old broiler chicks were vaccinated via the oculo-nasal route with different live infectious bronchitis virus (IBV) vaccines: Massachusetts (Mass), 793B, D274 or Arkansas (Ark). Clinical signs and gross lesions were evaluated. Five chicks from each group were humanely killed at intervals and their tracheas collected for ciliary activity assessment and for the detection of CD4+, CD8+ and IgA-bearing B cells by immunohistochemistry (IHC). Blood samples were collected at intervals for the detection of anti-IBV antibodies. At 21 days post-vaccination (dpv), protection conferred by different vaccination regimes against virulent M41, QX and 793B was assessed. All vaccination programmes were able to induce high levels of CD4+, CD8+ and IgA-bearing B cells in the trachea. Significantly higher levels of CD4+ and CD8+ expression were observed in the Mass ₂ + 793B ₂-vaccinated group compared to the other groups (subscripts indicate different manufacturers). Protection studies showed that the group of chicks vaccinated with Mass ₂ + 793B ₂ produced 92% ciliary protection against QX challenge; compared to 53%, 68% and 73% ciliary protection against the same challenge virus by Mass ₁ + D274, Mass ₁ + 793B ₁ and Mass ₃ + Ark, respectively. All vaccination programmes produced more than 85% ciliary protection against M41 and 793B challenges. It appears that the variable levels of protection provided by different heterologous live IBV vaccinations are dependent on the levels of local tracheal immunity induced by the respective vaccine combination. The Mass ₂ + 793B ₂ group showed the worst clinical signs, higher mortality and severe lesions following vaccination, but had the highest tracheal immune responses and demonstrated the best protection against all three challenge viruses. |
| doi_str_mv | 10.1080/03079457.2015.1137866 |
| format | Article |
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Clinical signs and gross lesions were evaluated. Five chicks from each group were humanely killed at intervals and their tracheas collected for ciliary activity assessment and for the detection of CD4+, CD8+ and IgA-bearing B cells by immunohistochemistry (IHC). Blood samples were collected at intervals for the detection of anti-IBV antibodies. At 21 days post-vaccination (dpv), protection conferred by different vaccination regimes against virulent M41, QX and 793B was assessed. All vaccination programmes were able to induce high levels of CD4+, CD8+ and IgA-bearing B cells in the trachea. Significantly higher levels of CD4+ and CD8+ expression were observed in the Mass ₂ + 793B ₂-vaccinated group compared to the other groups (subscripts indicate different manufacturers). Protection studies showed that the group of chicks vaccinated with Mass ₂ + 793B ₂ produced 92% ciliary protection against QX challenge; compared to 53%, 68% and 73% ciliary protection against the same challenge virus by Mass ₁ + D274, Mass ₁ + 793B ₁ and Mass ₃ + Ark, respectively. All vaccination programmes produced more than 85% ciliary protection against M41 and 793B challenges. It appears that the variable levels of protection provided by different heterologous live IBV vaccinations are dependent on the levels of local tracheal immunity induced by the respective vaccine combination. The Mass ₂ + 793B ₂ group showed the worst clinical signs, higher mortality and severe lesions following vaccination, but had the highest tracheal immune responses and demonstrated the best protection against all three challenge viruses.</description><identifier>ISSN: 1465-3338</identifier><identifier>ISSN: 0307-9457</identifier><identifier>EISSN: 1465-3338</identifier><identifier>DOI: 10.1080/03079457.2015.1137866</identifier><identifier>PMID: 26743315</identifier><language>eng</language><publisher>England: Taylor & Francis</publisher><subject>Animals ; Animals, Newborn ; Antibodies, Viral - blood ; broiler chicks ; Bronchitis ; CD4 antigen ; CD8 antigen ; Chickens ; Coronavirus Infections - prevention & control ; Coronavirus Infections - veterinary ; Enzyme-Linked Immunosorbent Assay - veterinary ; IBV ; immunity ; Immunity, Humoral ; Immunoglobulin A ; Immunohistochemistry ; Infectious bronchitis virus ; Infectious bronchitis virus - immunology ; Juveniles ; Lesions ; live vaccine ; Lymphocytes B ; Poultry ; Poultry Diseases - virology ; protection ; vaccination ; Vaccination - veterinary ; Vaccines ; Vaccines, Attenuated ; Viral Vaccines - immunology ; Viruses</subject><ispartof>Avian pathology, 2016-03, Vol.45 (2), p.169-177</ispartof><rights>2016 Houghton Trust Ltd 2016</rights><rights>2016 Houghton Trust Ltd</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c498t-2820e3286816ed14f6dd0af9beccaab495977d1793ea17c30146286d8caa3a093</citedby><cites>FETCH-LOGICAL-c498t-2820e3286816ed14f6dd0af9beccaab495977d1793ea17c30146286d8caa3a093</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26743315$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Awad, Faez</creatorcontrib><creatorcontrib>Hutton, Sally</creatorcontrib><creatorcontrib>Forrester, Anne</creatorcontrib><creatorcontrib>Baylis, Matthew</creatorcontrib><creatorcontrib>Ganapathy, Kannan</creatorcontrib><title>Heterologous live infectious bronchitis virus vaccination in day-old commercial broiler chicks: clinical signs, ciliary health, immune responses and protection against variant infectious bronchitis viruses</title><title>Avian pathology</title><addtitle>Avian Pathol</addtitle><description>Groups of one-day-old broiler chicks were vaccinated via the oculo-nasal route with different live infectious bronchitis virus (IBV) vaccines: Massachusetts (Mass), 793B, D274 or Arkansas (Ark). Clinical signs and gross lesions were evaluated. Five chicks from each group were humanely killed at intervals and their tracheas collected for ciliary activity assessment and for the detection of CD4+, CD8+ and IgA-bearing B cells by immunohistochemistry (IHC). Blood samples were collected at intervals for the detection of anti-IBV antibodies. At 21 days post-vaccination (dpv), protection conferred by different vaccination regimes against virulent M41, QX and 793B was assessed. All vaccination programmes were able to induce high levels of CD4+, CD8+ and IgA-bearing B cells in the trachea. Significantly higher levels of CD4+ and CD8+ expression were observed in the Mass ₂ + 793B ₂-vaccinated group compared to the other groups (subscripts indicate different manufacturers). Protection studies showed that the group of chicks vaccinated with Mass ₂ + 793B ₂ produced 92% ciliary protection against QX challenge; compared to 53%, 68% and 73% ciliary protection against the same challenge virus by Mass ₁ + D274, Mass ₁ + 793B ₁ and Mass ₃ + Ark, respectively. All vaccination programmes produced more than 85% ciliary protection against M41 and 793B challenges. It appears that the variable levels of protection provided by different heterologous live IBV vaccinations are dependent on the levels of local tracheal immunity induced by the respective vaccine combination. The Mass ₂ + 793B ₂ group showed the worst clinical signs, higher mortality and severe lesions following vaccination, but had the highest tracheal immune responses and demonstrated the best protection against all three challenge viruses.</description><subject>Animals</subject><subject>Animals, Newborn</subject><subject>Antibodies, Viral - blood</subject><subject>broiler chicks</subject><subject>Bronchitis</subject><subject>CD4 antigen</subject><subject>CD8 antigen</subject><subject>Chickens</subject><subject>Coronavirus Infections - prevention & control</subject><subject>Coronavirus Infections - veterinary</subject><subject>Enzyme-Linked Immunosorbent Assay - veterinary</subject><subject>IBV</subject><subject>immunity</subject><subject>Immunity, Humoral</subject><subject>Immunoglobulin A</subject><subject>Immunohistochemistry</subject><subject>Infectious bronchitis virus</subject><subject>Infectious bronchitis virus - immunology</subject><subject>Juveniles</subject><subject>Lesions</subject><subject>live vaccine</subject><subject>Lymphocytes B</subject><subject>Poultry</subject><subject>Poultry Diseases - virology</subject><subject>protection</subject><subject>vaccination</subject><subject>Vaccination - veterinary</subject><subject>Vaccines</subject><subject>Vaccines, Attenuated</subject><subject>Viral Vaccines - immunology</subject><subject>Viruses</subject><issn>1465-3338</issn><issn>0307-9457</issn><issn>1465-3338</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNks9u1DAQxiMEoqXwCIAlLhyaxY4Tx-kJVAFFqsQBerZm7cmui2MvtnfRPiTvhMNuEeIAnPxnft_MePxV1VNGF4xK-opy2g9t1y8ayroFY7yXQtyrTlkruppzLu__tj-pHqV0SykVXdc8rE4a0becs-60-n6FGWNwYRW2iTi7Q2L9iDrb-byMweu1zTaRnY3lYgdaWw8l6gtHDOzr4AzRYZowagtulliHkRSZ_pIuiHbWW10Cya58OifaOgtxT9YILq_PiZ2mrUcSMW2CT5gIeEM2MeSfPXgCK7A-5VI5WvD5b91helw9GMElfHJcz6qbd28_X17V1x_ff7h8c13rdpC5bmRDkTdSSCbQsHYUxlAYhyVqDbBsh27oe8P6gSOwXnNaBlloI0uUAx34WfXykLc0-nWLKavJJo3OgcfSmWLz19BO8P9BJR9YI-WMvvgDvQ3b6MtDZqplgxQNLVR3oHQMKUUc1SbaqYxUMapma6g7a6jZGupojaJ7dsy-XU5ofqnuvFCA1wegjDjECb6F6IzKsHchjhG8tknxf9V4fkgxQlCwikVx86kQghaqoaLnPwAmX9bY</recordid><startdate>20160303</startdate><enddate>20160303</enddate><creator>Awad, Faez</creator><creator>Hutton, Sally</creator><creator>Forrester, Anne</creator><creator>Baylis, Matthew</creator><creator>Ganapathy, Kannan</creator><general>Taylor & Francis</general><general>Taylor & Francis Ltd</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T5</scope><scope>7TM</scope><scope>7U7</scope><scope>7U9</scope><scope>C1K</scope><scope>F1W</scope><scope>H94</scope><scope>H95</scope><scope>K9.</scope><scope>L.G</scope><scope>M7N</scope><scope>7X8</scope></search><sort><creationdate>20160303</creationdate><title>Heterologous live infectious bronchitis virus vaccination in day-old commercial broiler chicks: clinical signs, ciliary health, immune responses and protection against variant infectious bronchitis viruses</title><author>Awad, Faez ; Hutton, Sally ; Forrester, Anne ; Baylis, Matthew ; Ganapathy, Kannan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c498t-2820e3286816ed14f6dd0af9beccaab495977d1793ea17c30146286d8caa3a093</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Animals</topic><topic>Animals, Newborn</topic><topic>Antibodies, Viral - blood</topic><topic>broiler chicks</topic><topic>Bronchitis</topic><topic>CD4 antigen</topic><topic>CD8 antigen</topic><topic>Chickens</topic><topic>Coronavirus Infections - prevention & control</topic><topic>Coronavirus Infections - veterinary</topic><topic>Enzyme-Linked Immunosorbent Assay - veterinary</topic><topic>IBV</topic><topic>immunity</topic><topic>Immunity, Humoral</topic><topic>Immunoglobulin A</topic><topic>Immunohistochemistry</topic><topic>Infectious bronchitis virus</topic><topic>Infectious bronchitis virus - immunology</topic><topic>Juveniles</topic><topic>Lesions</topic><topic>live vaccine</topic><topic>Lymphocytes B</topic><topic>Poultry</topic><topic>Poultry Diseases - virology</topic><topic>protection</topic><topic>vaccination</topic><topic>Vaccination - veterinary</topic><topic>Vaccines</topic><topic>Vaccines, Attenuated</topic><topic>Viral Vaccines - immunology</topic><topic>Viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Awad, Faez</creatorcontrib><creatorcontrib>Hutton, Sally</creatorcontrib><creatorcontrib>Forrester, Anne</creatorcontrib><creatorcontrib>Baylis, Matthew</creatorcontrib><creatorcontrib>Ganapathy, Kannan</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Immunology Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><jtitle>Avian pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Awad, Faez</au><au>Hutton, Sally</au><au>Forrester, Anne</au><au>Baylis, Matthew</au><au>Ganapathy, Kannan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Heterologous live infectious bronchitis virus vaccination in day-old commercial broiler chicks: clinical signs, ciliary health, immune responses and protection against variant infectious bronchitis viruses</atitle><jtitle>Avian pathology</jtitle><addtitle>Avian Pathol</addtitle><date>2016-03-03</date><risdate>2016</risdate><volume>45</volume><issue>2</issue><spage>169</spage><epage>177</epage><pages>169-177</pages><issn>1465-3338</issn><issn>0307-9457</issn><eissn>1465-3338</eissn><abstract>Groups of one-day-old broiler chicks were vaccinated via the oculo-nasal route with different live infectious bronchitis virus (IBV) vaccines: Massachusetts (Mass), 793B, D274 or Arkansas (Ark). Clinical signs and gross lesions were evaluated. Five chicks from each group were humanely killed at intervals and their tracheas collected for ciliary activity assessment and for the detection of CD4+, CD8+ and IgA-bearing B cells by immunohistochemistry (IHC). Blood samples were collected at intervals for the detection of anti-IBV antibodies. At 21 days post-vaccination (dpv), protection conferred by different vaccination regimes against virulent M41, QX and 793B was assessed. All vaccination programmes were able to induce high levels of CD4+, CD8+ and IgA-bearing B cells in the trachea. Significantly higher levels of CD4+ and CD8+ expression were observed in the Mass ₂ + 793B ₂-vaccinated group compared to the other groups (subscripts indicate different manufacturers). Protection studies showed that the group of chicks vaccinated with Mass ₂ + 793B ₂ produced 92% ciliary protection against QX challenge; compared to 53%, 68% and 73% ciliary protection against the same challenge virus by Mass ₁ + D274, Mass ₁ + 793B ₁ and Mass ₃ + Ark, respectively. All vaccination programmes produced more than 85% ciliary protection against M41 and 793B challenges. It appears that the variable levels of protection provided by different heterologous live IBV vaccinations are dependent on the levels of local tracheal immunity induced by the respective vaccine combination. The Mass ₂ + 793B ₂ group showed the worst clinical signs, higher mortality and severe lesions following vaccination, but had the highest tracheal immune responses and demonstrated the best protection against all three challenge viruses.</abstract><cop>England</cop><pub>Taylor & Francis</pub><pmid>26743315</pmid><doi>10.1080/03079457.2015.1137866</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Animals, Newborn Antibodies, Viral - blood broiler chicks Bronchitis CD4 antigen CD8 antigen Chickens Coronavirus Infections - prevention & control Coronavirus Infections - veterinary Enzyme-Linked Immunosorbent Assay - veterinary IBV immunity Immunity, Humoral Immunoglobulin A Immunohistochemistry Infectious bronchitis virus Infectious bronchitis virus - immunology Juveniles Lesions live vaccine Lymphocytes B Poultry Poultry Diseases - virology protection vaccination Vaccination - veterinary Vaccines Vaccines, Attenuated Viral Vaccines - immunology Viruses |
| title | Heterologous live infectious bronchitis virus vaccination in day-old commercial broiler chicks: clinical signs, ciliary health, immune responses and protection against variant infectious bronchitis viruses |
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