In Vivo Evaluation of a Bombesin Analogue Labeled with Ga-68 and Co-55/57
Purpose The purpose of this study was to apply an analogue of bombesin, NOTA-AMBA, labeled with Co-55 or Ga-68, for preclinical imaging of prostate cancer. Procedures The peptide NOTA-AMBA was labeled with Ga-68 or Co-55 by microwave irradiation. Biodistribution in xenograft mice (PC3) was performed...
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| Veröffentlicht in: | Molecular imaging and biology 2016-06, Vol.18 (3), p.368-376 |
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| creator | Dam, Johan Hygum Olsen, Birgitte Brinkmann Baun, Christina Høilund-Carlsen, Poul-Flemming Thisgaard, Helge |
| description | Purpose
The purpose of this study was to apply an analogue of bombesin, NOTA-AMBA, labeled with Co-55 or Ga-68, for preclinical imaging of prostate cancer.
Procedures
The peptide NOTA-AMBA was labeled with Ga-68 or Co-55 by microwave irradiation. Biodistribution in xenograft mice (PC3) was performed at 1, 4, and 24 h (only cobalt at 24 h) using a fixed amount of peptide. Four weeks post-inoculation, xenograft mice were positron emission tomography/X-ray computed tomography scanned after tail vein injection of [
68
Ga]NOTA-AMBA or [
55
Co]NOTA-AMBA.
Results
Labeling with Ga-68 and Co-55/57 was achieved in yields greater than 90 %. A radiochemical purity (RCP) of 95 and 90 % were obtained for Ga-68 and Co-55, respectively. Both radiopeptides showed high uptake in the intestines, stomach, pancreas, and in the tumor ([
68
Ga]NOTA-AMBA, 10.3 %ID/g at 1 h to 6.4 %ID/g at 4 h; [
57
Co]NOTA-AMBA, 8.2 %ID/g at 1 h to 5.3%ID/g at 24 h). Normal tissue cleared over time improving tumor-to-background ratios.
Conclusions
NOTA-AMBA was labeled in high yields and RCP with Ga-68 and Co-55/57. High tumor uptake in a subcutaneous mouse prostate cancer model was observed. At 24 h, [
55/57
Co]NOTA-AMBA showed better tumor-to-organ ratios than [
68
Ga]NOTA-AMBA at both 1 and 4 h post-injection. Hence, for imaging, [
55
Co]NOTA-AMBA was found to be superior compared to [
68
Ga]NOTA-AMBA. |
| doi_str_mv | 10.1007/s11307-015-0911-z |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1794503107</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>4058930721</sourcerecordid><originalsourceid>FETCH-LOGICAL-c405t-96d917771f0ccc041757ffc61449c66760d9ceeedef2547ad287e5001ab810dc3</originalsourceid><addsrcrecordid>eNqNkU1LAzEQhoMoflR_gBcJePESO5PdJLtHLbUWCl7Ua0iz2bqy3dRNt2J_vSmtIoLgKQN55p1JHkLOEa4RQPUDYgKKAQoGOSJb75FjzCQwDsD3Yy0SyVAm_IichPAKgAp5ckiOuBQSgWfHZDxu6HO18nS4MnVnlpVvqC-pobd-PnWhauhNY2o_6xydmKmrXUHfq-ULHRkmM2qagg48E6Iv1Ck5KE0d3Nnu7JGnu-Hj4J5NHkbjwc2E2RTEkuWyyFEphSVYayFFJVRZWolpmlsplYQit865wpVcpMoUPFNOxNXNNEMobNIjV9vcRevfOheWel4F6-raNM53QaPKUwEJgvoPGj8KEy4jevkLffVdG5--obI8yYTMNxRuKdv6EFpX6kVbzU37oRH0RoneKtFRid4o0evYc7FL7qZzV3x3fDmIAN8CIV41M9f-GP1n6icEwJIR</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1789385696</pqid></control><display><type>article</type><title>In Vivo Evaluation of a Bombesin Analogue Labeled with Ga-68 and Co-55/57</title><source>MEDLINE</source><source>SpringerLink Journals</source><creator>Dam, Johan Hygum ; Olsen, Birgitte Brinkmann ; Baun, Christina ; Høilund-Carlsen, Poul-Flemming ; Thisgaard, Helge</creator><creatorcontrib>Dam, Johan Hygum ; Olsen, Birgitte Brinkmann ; Baun, Christina ; Høilund-Carlsen, Poul-Flemming ; Thisgaard, Helge</creatorcontrib><description>Purpose
The purpose of this study was to apply an analogue of bombesin, NOTA-AMBA, labeled with Co-55 or Ga-68, for preclinical imaging of prostate cancer.
Procedures
The peptide NOTA-AMBA was labeled with Ga-68 or Co-55 by microwave irradiation. Biodistribution in xenograft mice (PC3) was performed at 1, 4, and 24 h (only cobalt at 24 h) using a fixed amount of peptide. Four weeks post-inoculation, xenograft mice were positron emission tomography/X-ray computed tomography scanned after tail vein injection of [
68
Ga]NOTA-AMBA or [
55
Co]NOTA-AMBA.
Results
Labeling with Ga-68 and Co-55/57 was achieved in yields greater than 90 %. A radiochemical purity (RCP) of 95 and 90 % were obtained for Ga-68 and Co-55, respectively. Both radiopeptides showed high uptake in the intestines, stomach, pancreas, and in the tumor ([
68
Ga]NOTA-AMBA, 10.3 %ID/g at 1 h to 6.4 %ID/g at 4 h; [
57
Co]NOTA-AMBA, 8.2 %ID/g at 1 h to 5.3%ID/g at 24 h). Normal tissue cleared over time improving tumor-to-background ratios.
Conclusions
NOTA-AMBA was labeled in high yields and RCP with Ga-68 and Co-55/57. High tumor uptake in a subcutaneous mouse prostate cancer model was observed. At 24 h, [
55/57
Co]NOTA-AMBA showed better tumor-to-organ ratios than [
68
Ga]NOTA-AMBA at both 1 and 4 h post-injection. Hence, for imaging, [
55
Co]NOTA-AMBA was found to be superior compared to [
68
Ga]NOTA-AMBA.</description><identifier>ISSN: 1536-1632</identifier><identifier>EISSN: 1860-2002</identifier><identifier>DOI: 10.1007/s11307-015-0911-z</identifier><identifier>PMID: 26561028</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Animals ; Binding, Competitive ; Bombesin - analogs & derivatives ; Cell Line, Tumor ; Chromatography, High Pressure Liquid ; Cobalt Radioisotopes - chemistry ; Gallium Radioisotopes - chemistry ; Humans ; Imaging ; Inhibitory Concentration 50 ; Male ; Medicine ; Medicine & Public Health ; Mice, SCID ; Peptides - blood ; Peptides - chemistry ; Positron-Emission Tomography ; Protein Stability ; Radiology ; Radiopharmaceuticals - chemistry ; Research Article ; Tissue Distribution ; Tomography, X-Ray Computed ; Xenograft Model Antitumor Assays</subject><ispartof>Molecular imaging and biology, 2016-06, Vol.18 (3), p.368-376</ispartof><rights>World Molecular Imaging Society 2015</rights><rights>World Molecular Imaging Society 2016</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c405t-96d917771f0ccc041757ffc61449c66760d9ceeedef2547ad287e5001ab810dc3</citedby><cites>FETCH-LOGICAL-c405t-96d917771f0ccc041757ffc61449c66760d9ceeedef2547ad287e5001ab810dc3</cites><orcidid>0000-0001-6577-7011</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11307-015-0911-z$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11307-015-0911-z$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26561028$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Dam, Johan Hygum</creatorcontrib><creatorcontrib>Olsen, Birgitte Brinkmann</creatorcontrib><creatorcontrib>Baun, Christina</creatorcontrib><creatorcontrib>Høilund-Carlsen, Poul-Flemming</creatorcontrib><creatorcontrib>Thisgaard, Helge</creatorcontrib><title>In Vivo Evaluation of a Bombesin Analogue Labeled with Ga-68 and Co-55/57</title><title>Molecular imaging and biology</title><addtitle>Mol Imaging Biol</addtitle><addtitle>Mol Imaging Biol</addtitle><description>Purpose
The purpose of this study was to apply an analogue of bombesin, NOTA-AMBA, labeled with Co-55 or Ga-68, for preclinical imaging of prostate cancer.
Procedures
The peptide NOTA-AMBA was labeled with Ga-68 or Co-55 by microwave irradiation. Biodistribution in xenograft mice (PC3) was performed at 1, 4, and 24 h (only cobalt at 24 h) using a fixed amount of peptide. Four weeks post-inoculation, xenograft mice were positron emission tomography/X-ray computed tomography scanned after tail vein injection of [
68
Ga]NOTA-AMBA or [
55
Co]NOTA-AMBA.
Results
Labeling with Ga-68 and Co-55/57 was achieved in yields greater than 90 %. A radiochemical purity (RCP) of 95 and 90 % were obtained for Ga-68 and Co-55, respectively. Both radiopeptides showed high uptake in the intestines, stomach, pancreas, and in the tumor ([
68
Ga]NOTA-AMBA, 10.3 %ID/g at 1 h to 6.4 %ID/g at 4 h; [
57
Co]NOTA-AMBA, 8.2 %ID/g at 1 h to 5.3%ID/g at 24 h). Normal tissue cleared over time improving tumor-to-background ratios.
Conclusions
NOTA-AMBA was labeled in high yields and RCP with Ga-68 and Co-55/57. High tumor uptake in a subcutaneous mouse prostate cancer model was observed. At 24 h, [
55/57
Co]NOTA-AMBA showed better tumor-to-organ ratios than [
68
Ga]NOTA-AMBA at both 1 and 4 h post-injection. Hence, for imaging, [
55
Co]NOTA-AMBA was found to be superior compared to [
68
Ga]NOTA-AMBA.</description><subject>Animals</subject><subject>Binding, Competitive</subject><subject>Bombesin - analogs & derivatives</subject><subject>Cell Line, Tumor</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Cobalt Radioisotopes - chemistry</subject><subject>Gallium Radioisotopes - chemistry</subject><subject>Humans</subject><subject>Imaging</subject><subject>Inhibitory Concentration 50</subject><subject>Male</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Mice, SCID</subject><subject>Peptides - blood</subject><subject>Peptides - chemistry</subject><subject>Positron-Emission Tomography</subject><subject>Protein Stability</subject><subject>Radiology</subject><subject>Radiopharmaceuticals - chemistry</subject><subject>Research Article</subject><subject>Tissue Distribution</subject><subject>Tomography, X-Ray Computed</subject><subject>Xenograft Model Antitumor Assays</subject><issn>1536-1632</issn><issn>1860-2002</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqNkU1LAzEQhoMoflR_gBcJePESO5PdJLtHLbUWCl7Ua0iz2bqy3dRNt2J_vSmtIoLgKQN55p1JHkLOEa4RQPUDYgKKAQoGOSJb75FjzCQwDsD3Yy0SyVAm_IichPAKgAp5ckiOuBQSgWfHZDxu6HO18nS4MnVnlpVvqC-pobd-PnWhauhNY2o_6xydmKmrXUHfq-ULHRkmM2qagg48E6Iv1Ck5KE0d3Nnu7JGnu-Hj4J5NHkbjwc2E2RTEkuWyyFEphSVYayFFJVRZWolpmlsplYQit865wpVcpMoUPFNOxNXNNEMobNIjV9vcRevfOheWel4F6-raNM53QaPKUwEJgvoPGj8KEy4jevkLffVdG5--obI8yYTMNxRuKdv6EFpX6kVbzU37oRH0RoneKtFRid4o0evYc7FL7qZzV3x3fDmIAN8CIV41M9f-GP1n6icEwJIR</recordid><startdate>20160601</startdate><enddate>20160601</enddate><creator>Dam, Johan Hygum</creator><creator>Olsen, Birgitte Brinkmann</creator><creator>Baun, Christina</creator><creator>Høilund-Carlsen, Poul-Flemming</creator><creator>Thisgaard, Helge</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QO</scope><scope>7RV</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB0</scope><scope>L6V</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-6577-7011</orcidid></search><sort><creationdate>20160601</creationdate><title>In Vivo Evaluation of a Bombesin Analogue Labeled with Ga-68 and Co-55/57</title><author>Dam, Johan Hygum ; Olsen, Birgitte Brinkmann ; Baun, Christina ; Høilund-Carlsen, Poul-Flemming ; Thisgaard, Helge</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c405t-96d917771f0ccc041757ffc61449c66760d9ceeedef2547ad287e5001ab810dc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Animals</topic><topic>Binding, Competitive</topic><topic>Bombesin - analogs & derivatives</topic><topic>Cell Line, Tumor</topic><topic>Chromatography, High Pressure Liquid</topic><topic>Cobalt Radioisotopes - chemistry</topic><topic>Gallium Radioisotopes - chemistry</topic><topic>Humans</topic><topic>Imaging</topic><topic>Inhibitory Concentration 50</topic><topic>Male</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Mice, SCID</topic><topic>Peptides - blood</topic><topic>Peptides - chemistry</topic><topic>Positron-Emission Tomography</topic><topic>Protein Stability</topic><topic>Radiology</topic><topic>Radiopharmaceuticals - chemistry</topic><topic>Research Article</topic><topic>Tissue Distribution</topic><topic>Tomography, X-Ray Computed</topic><topic>Xenograft Model Antitumor Assays</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dam, Johan Hygum</creatorcontrib><creatorcontrib>Olsen, Birgitte Brinkmann</creatorcontrib><creatorcontrib>Baun, Christina</creatorcontrib><creatorcontrib>Høilund-Carlsen, Poul-Flemming</creatorcontrib><creatorcontrib>Thisgaard, Helge</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular imaging and biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dam, Johan Hygum</au><au>Olsen, Birgitte Brinkmann</au><au>Baun, Christina</au><au>Høilund-Carlsen, Poul-Flemming</au><au>Thisgaard, Helge</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In Vivo Evaluation of a Bombesin Analogue Labeled with Ga-68 and Co-55/57</atitle><jtitle>Molecular imaging and biology</jtitle><stitle>Mol Imaging Biol</stitle><addtitle>Mol Imaging Biol</addtitle><date>2016-06-01</date><risdate>2016</risdate><volume>18</volume><issue>3</issue><spage>368</spage><epage>376</epage><pages>368-376</pages><issn>1536-1632</issn><eissn>1860-2002</eissn><abstract>Purpose
The purpose of this study was to apply an analogue of bombesin, NOTA-AMBA, labeled with Co-55 or Ga-68, for preclinical imaging of prostate cancer.
Procedures
The peptide NOTA-AMBA was labeled with Ga-68 or Co-55 by microwave irradiation. Biodistribution in xenograft mice (PC3) was performed at 1, 4, and 24 h (only cobalt at 24 h) using a fixed amount of peptide. Four weeks post-inoculation, xenograft mice were positron emission tomography/X-ray computed tomography scanned after tail vein injection of [
68
Ga]NOTA-AMBA or [
55
Co]NOTA-AMBA.
Results
Labeling with Ga-68 and Co-55/57 was achieved in yields greater than 90 %. A radiochemical purity (RCP) of 95 and 90 % were obtained for Ga-68 and Co-55, respectively. Both radiopeptides showed high uptake in the intestines, stomach, pancreas, and in the tumor ([
68
Ga]NOTA-AMBA, 10.3 %ID/g at 1 h to 6.4 %ID/g at 4 h; [
57
Co]NOTA-AMBA, 8.2 %ID/g at 1 h to 5.3%ID/g at 24 h). Normal tissue cleared over time improving tumor-to-background ratios.
Conclusions
NOTA-AMBA was labeled in high yields and RCP with Ga-68 and Co-55/57. High tumor uptake in a subcutaneous mouse prostate cancer model was observed. At 24 h, [
55/57
Co]NOTA-AMBA showed better tumor-to-organ ratios than [
68
Ga]NOTA-AMBA at both 1 and 4 h post-injection. Hence, for imaging, [
55
Co]NOTA-AMBA was found to be superior compared to [
68
Ga]NOTA-AMBA.</abstract><cop>New York</cop><pub>Springer US</pub><pmid>26561028</pmid><doi>10.1007/s11307-015-0911-z</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0001-6577-7011</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1536-1632 |
| ispartof | Molecular imaging and biology, 2016-06, Vol.18 (3), p.368-376 |
| issn | 1536-1632 1860-2002 |
| language | eng |
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| source | MEDLINE; SpringerLink Journals |
| subjects | Animals Binding, Competitive Bombesin - analogs & derivatives Cell Line, Tumor Chromatography, High Pressure Liquid Cobalt Radioisotopes - chemistry Gallium Radioisotopes - chemistry Humans Imaging Inhibitory Concentration 50 Male Medicine Medicine & Public Health Mice, SCID Peptides - blood Peptides - chemistry Positron-Emission Tomography Protein Stability Radiology Radiopharmaceuticals - chemistry Research Article Tissue Distribution Tomography, X-Ray Computed Xenograft Model Antitumor Assays |
| title | In Vivo Evaluation of a Bombesin Analogue Labeled with Ga-68 and Co-55/57 |
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