Cell-free Epstein-Barr virus-DNA in patients with nasopharyngeal carcinoma: Plasma versus urine
ABSTRACT Background The purpose of this study was to explore urinary Epstein–Barr virus (EBV)‐DNA as a potential biomarker in patients with nasopharyngeal carcinoma (NPC). Methods EBV‐DNA copies were estimated in plasma/urine of patients with NPC (n = 76) by real‐time polymerase chain reaction (PCR)...
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| creator | Sengar, Manju Chorghe, Siddhesh Jadhav, Kamini Singh, Shikha Laskar, Sarbani Ghosh Pai, Prathamesh Aggarwal, Jai Prakash D'Cruz, Anil Chaturvedi, Pankaj Deshpande, Mandar Chaukar, Devendra Budrukkar, Ashwini Gupta, Tejpal Murthy, Vedang Kane, Shubhada Thakur, Meenakshi Rangarajan, Venkatesh Kannan, Sadhana Shet, Tanuja Kode, Jyoti |
| description | ABSTRACT
Background
The purpose of this study was to explore urinary Epstein–Barr virus (EBV)‐DNA as a potential biomarker in patients with nasopharyngeal carcinoma (NPC).
Methods
EBV‐DNA copies were estimated in plasma/urine of patients with NPC (n = 76) by real‐time polymerase chain reaction (PCR) at baseline, during therapy, and at follow‐up. Their correlation with EBV‐RNA expression in tissues (n = 53) was used to assess sensitivity and specificity of plasma/urine EBV‐DNA. Correlation of urine and plasma EBV‐DNA with each other and with radiological response was evaluated.
Results
This study demonstrated that urine EBV‐DNA has high sensitivity (96%) at diagnosis and it correlates well with plasma EBV‐DNA at baseline and after neoadjuvant chemotherapy. The EBV‐DNA copies reduced significantly with therapy (plasma: p < .001; urine: p = .011). Patients with low EBV‐DNA copies demonstrated improved survival (plasma: p = .023; urine: p = .083).
Conclusion
Plasma EBV‐DNA is a good prognostic marker, whereas further study on a larger cohort may help in developing urine EBV‐DNA as a surrogate prognostic marker for patients with NPC. © 2015 Wiley Periodicals, Inc. Head Neck 38: E1666–E1673, 2016 |
| doi_str_mv | 10.1002/hed.24297 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1794498384</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1784745541</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3967-cd2bc448d5be06f31cd3fda0b4d65dea9f6e4c273d8618f563a552d1e0a6dec3</originalsourceid><addsrcrecordid>eNqNkUlvFDEQhS1ERELgwB9APsLBid1em1uYTBYUTTiMAHGxPHY146Q37O4s_z6dTIYbUk5VKn3vSe8VQh8YPWCUFodrCAeFKEr9Cu0xWmpCudCvH3fBCada7KK3OV9RSrkSxRu0WyiltDF8D9kZ1DWpEgCe93mA2JKvLiV8E9OYyfHiCMcW926I0A4Z38ZhjVuXu37t0n37B1yNvUs-tl3jvuDvtcuNwzeQ8pjxmGIL79BO5eoM75_nPlqezJezM3JxeXo-O7ognpdKEx-KlRfCBLkCqirOfOBVcHQlgpIBXFkpEL7QPBjFTCUVd1IWgQF1KoDn--jTxrZP3d8R8mCbmP0UzbXQjdkyXQpRGm7EC1AjtJBSsAn9vEF96nJOUNk-xWZKbhm1j83bqXn71PzEfny2HVfNdN2S26on4HAD3MYa7v_vZM_mx1tLslHE6TF3_xQuXVuluZb25-LUflv8WP7-ZaiV_AEr5pz_</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1784745541</pqid></control><display><type>article</type><title>Cell-free Epstein-Barr virus-DNA in patients with nasopharyngeal carcinoma: Plasma versus urine</title><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><creator>Sengar, Manju ; Chorghe, Siddhesh ; Jadhav, Kamini ; Singh, Shikha ; Laskar, Sarbani Ghosh ; Pai, Prathamesh ; Aggarwal, Jai Prakash ; D'Cruz, Anil ; Chaturvedi, Pankaj ; Deshpande, Mandar ; Chaukar, Devendra ; Budrukkar, Ashwini ; Gupta, Tejpal ; Murthy, Vedang ; Kane, Shubhada ; Thakur, Meenakshi ; Rangarajan, Venkatesh ; Kannan, Sadhana ; Shet, Tanuja ; Kode, Jyoti</creator><creatorcontrib>Sengar, Manju ; Chorghe, Siddhesh ; Jadhav, Kamini ; Singh, Shikha ; Laskar, Sarbani Ghosh ; Pai, Prathamesh ; Aggarwal, Jai Prakash ; D'Cruz, Anil ; Chaturvedi, Pankaj ; Deshpande, Mandar ; Chaukar, Devendra ; Budrukkar, Ashwini ; Gupta, Tejpal ; Murthy, Vedang ; Kane, Shubhada ; Thakur, Meenakshi ; Rangarajan, Venkatesh ; Kannan, Sadhana ; Shet, Tanuja ; Kode, Jyoti</creatorcontrib><description>ABSTRACT
Background
The purpose of this study was to explore urinary Epstein–Barr virus (EBV)‐DNA as a potential biomarker in patients with nasopharyngeal carcinoma (NPC).
Methods
EBV‐DNA copies were estimated in plasma/urine of patients with NPC (n = 76) by real‐time polymerase chain reaction (PCR) at baseline, during therapy, and at follow‐up. Their correlation with EBV‐RNA expression in tissues (n = 53) was used to assess sensitivity and specificity of plasma/urine EBV‐DNA. Correlation of urine and plasma EBV‐DNA with each other and with radiological response was evaluated.
Results
This study demonstrated that urine EBV‐DNA has high sensitivity (96%) at diagnosis and it correlates well with plasma EBV‐DNA at baseline and after neoadjuvant chemotherapy. The EBV‐DNA copies reduced significantly with therapy (plasma: p < .001; urine: p = .011). Patients with low EBV‐DNA copies demonstrated improved survival (plasma: p = .023; urine: p = .083).
Conclusion
Plasma EBV‐DNA is a good prognostic marker, whereas further study on a larger cohort may help in developing urine EBV‐DNA as a surrogate prognostic marker for patients with NPC. © 2015 Wiley Periodicals, Inc. Head Neck 38: E1666–E1673, 2016</description><identifier>ISSN: 1043-3074</identifier><identifier>EISSN: 1097-0347</identifier><identifier>DOI: 10.1002/hed.24297</identifier><identifier>PMID: 26667883</identifier><language>eng</language><publisher>United States: Blackwell Publishing Ltd</publisher><subject>Adolescent ; Adult ; Aged ; Carcinoma - virology ; DNA, Viral - blood ; DNA, Viral - urine ; Epstein-Barr encoding region in situ hybridization (EBER-ISH) ; Epstein-Barr virus ; Epstein-Barr Virus Infections - diagnosis ; Female ; Herpesvirus 4, Human ; Humans ; Male ; Middle Aged ; Nasopharyngeal Carcinoma ; Nasopharyngeal Neoplasms - virology ; plasma/urinary Epstein-Barr virus (EBV)-DNA ; positron emission tomography (PET)-CT ; Prognosis ; real-time polymerase chain reaction (PCR) ; Sensitivity and Specificity ; Young Adult</subject><ispartof>Head & neck, 2016-04, Vol.38 (S1), p.E1666-E1673</ispartof><rights>2015 Wiley Periodicals, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3967-cd2bc448d5be06f31cd3fda0b4d65dea9f6e4c273d8618f563a552d1e0a6dec3</citedby><cites>FETCH-LOGICAL-c3967-cd2bc448d5be06f31cd3fda0b4d65dea9f6e4c273d8618f563a552d1e0a6dec3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fhed.24297$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fhed.24297$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26667883$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sengar, Manju</creatorcontrib><creatorcontrib>Chorghe, Siddhesh</creatorcontrib><creatorcontrib>Jadhav, Kamini</creatorcontrib><creatorcontrib>Singh, Shikha</creatorcontrib><creatorcontrib>Laskar, Sarbani Ghosh</creatorcontrib><creatorcontrib>Pai, Prathamesh</creatorcontrib><creatorcontrib>Aggarwal, Jai Prakash</creatorcontrib><creatorcontrib>D'Cruz, Anil</creatorcontrib><creatorcontrib>Chaturvedi, Pankaj</creatorcontrib><creatorcontrib>Deshpande, Mandar</creatorcontrib><creatorcontrib>Chaukar, Devendra</creatorcontrib><creatorcontrib>Budrukkar, Ashwini</creatorcontrib><creatorcontrib>Gupta, Tejpal</creatorcontrib><creatorcontrib>Murthy, Vedang</creatorcontrib><creatorcontrib>Kane, Shubhada</creatorcontrib><creatorcontrib>Thakur, Meenakshi</creatorcontrib><creatorcontrib>Rangarajan, Venkatesh</creatorcontrib><creatorcontrib>Kannan, Sadhana</creatorcontrib><creatorcontrib>Shet, Tanuja</creatorcontrib><creatorcontrib>Kode, Jyoti</creatorcontrib><title>Cell-free Epstein-Barr virus-DNA in patients with nasopharyngeal carcinoma: Plasma versus urine</title><title>Head & neck</title><addtitle>Head Neck</addtitle><description>ABSTRACT
Background
The purpose of this study was to explore urinary Epstein–Barr virus (EBV)‐DNA as a potential biomarker in patients with nasopharyngeal carcinoma (NPC).
Methods
EBV‐DNA copies were estimated in plasma/urine of patients with NPC (n = 76) by real‐time polymerase chain reaction (PCR) at baseline, during therapy, and at follow‐up. Their correlation with EBV‐RNA expression in tissues (n = 53) was used to assess sensitivity and specificity of plasma/urine EBV‐DNA. Correlation of urine and plasma EBV‐DNA with each other and with radiological response was evaluated.
Results
This study demonstrated that urine EBV‐DNA has high sensitivity (96%) at diagnosis and it correlates well with plasma EBV‐DNA at baseline and after neoadjuvant chemotherapy. The EBV‐DNA copies reduced significantly with therapy (plasma: p < .001; urine: p = .011). Patients with low EBV‐DNA copies demonstrated improved survival (plasma: p = .023; urine: p = .083).
Conclusion
Plasma EBV‐DNA is a good prognostic marker, whereas further study on a larger cohort may help in developing urine EBV‐DNA as a surrogate prognostic marker for patients with NPC. © 2015 Wiley Periodicals, Inc. Head Neck 38: E1666–E1673, 2016</description><subject>Adolescent</subject><subject>Adult</subject><subject>Aged</subject><subject>Carcinoma - virology</subject><subject>DNA, Viral - blood</subject><subject>DNA, Viral - urine</subject><subject>Epstein-Barr encoding region in situ hybridization (EBER-ISH)</subject><subject>Epstein-Barr virus</subject><subject>Epstein-Barr Virus Infections - diagnosis</subject><subject>Female</subject><subject>Herpesvirus 4, Human</subject><subject>Humans</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Nasopharyngeal Carcinoma</subject><subject>Nasopharyngeal Neoplasms - virology</subject><subject>plasma/urinary Epstein-Barr virus (EBV)-DNA</subject><subject>positron emission tomography (PET)-CT</subject><subject>Prognosis</subject><subject>real-time polymerase chain reaction (PCR)</subject><subject>Sensitivity and Specificity</subject><subject>Young Adult</subject><issn>1043-3074</issn><issn>1097-0347</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkUlvFDEQhS1ERELgwB9APsLBid1em1uYTBYUTTiMAHGxPHY146Q37O4s_z6dTIYbUk5VKn3vSe8VQh8YPWCUFodrCAeFKEr9Cu0xWmpCudCvH3fBCada7KK3OV9RSrkSxRu0WyiltDF8D9kZ1DWpEgCe93mA2JKvLiV8E9OYyfHiCMcW926I0A4Z38ZhjVuXu37t0n37B1yNvUs-tl3jvuDvtcuNwzeQ8pjxmGIL79BO5eoM75_nPlqezJezM3JxeXo-O7ognpdKEx-KlRfCBLkCqirOfOBVcHQlgpIBXFkpEL7QPBjFTCUVd1IWgQF1KoDn--jTxrZP3d8R8mCbmP0UzbXQjdkyXQpRGm7EC1AjtJBSsAn9vEF96nJOUNk-xWZKbhm1j83bqXn71PzEfny2HVfNdN2S26on4HAD3MYa7v_vZM_mx1tLslHE6TF3_xQuXVuluZb25-LUflv8WP7-ZaiV_AEr5pz_</recordid><startdate>201604</startdate><enddate>201604</enddate><creator>Sengar, Manju</creator><creator>Chorghe, Siddhesh</creator><creator>Jadhav, Kamini</creator><creator>Singh, Shikha</creator><creator>Laskar, Sarbani Ghosh</creator><creator>Pai, Prathamesh</creator><creator>Aggarwal, Jai Prakash</creator><creator>D'Cruz, Anil</creator><creator>Chaturvedi, Pankaj</creator><creator>Deshpande, Mandar</creator><creator>Chaukar, Devendra</creator><creator>Budrukkar, Ashwini</creator><creator>Gupta, Tejpal</creator><creator>Murthy, Vedang</creator><creator>Kane, Shubhada</creator><creator>Thakur, Meenakshi</creator><creator>Rangarajan, Venkatesh</creator><creator>Kannan, Sadhana</creator><creator>Shet, Tanuja</creator><creator>Kode, Jyoti</creator><general>Blackwell Publishing Ltd</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7TM</scope><scope>7U9</scope><scope>H94</scope></search><sort><creationdate>201604</creationdate><title>Cell-free Epstein-Barr virus-DNA in patients with nasopharyngeal carcinoma: Plasma versus urine</title><author>Sengar, Manju ; Chorghe, Siddhesh ; Jadhav, Kamini ; Singh, Shikha ; Laskar, Sarbani Ghosh ; Pai, Prathamesh ; Aggarwal, Jai Prakash ; D'Cruz, Anil ; Chaturvedi, Pankaj ; Deshpande, Mandar ; Chaukar, Devendra ; Budrukkar, Ashwini ; Gupta, Tejpal ; Murthy, Vedang ; Kane, Shubhada ; Thakur, Meenakshi ; Rangarajan, Venkatesh ; Kannan, Sadhana ; Shet, Tanuja ; Kode, Jyoti</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3967-cd2bc448d5be06f31cd3fda0b4d65dea9f6e4c273d8618f563a552d1e0a6dec3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Aged</topic><topic>Carcinoma - virology</topic><topic>DNA, Viral - blood</topic><topic>DNA, Viral - urine</topic><topic>Epstein-Barr encoding region in situ hybridization (EBER-ISH)</topic><topic>Epstein-Barr virus</topic><topic>Epstein-Barr Virus Infections - diagnosis</topic><topic>Female</topic><topic>Herpesvirus 4, Human</topic><topic>Humans</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Nasopharyngeal Carcinoma</topic><topic>Nasopharyngeal Neoplasms - virology</topic><topic>plasma/urinary Epstein-Barr virus (EBV)-DNA</topic><topic>positron emission tomography (PET)-CT</topic><topic>Prognosis</topic><topic>real-time polymerase chain reaction (PCR)</topic><topic>Sensitivity and Specificity</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sengar, Manju</creatorcontrib><creatorcontrib>Chorghe, Siddhesh</creatorcontrib><creatorcontrib>Jadhav, Kamini</creatorcontrib><creatorcontrib>Singh, Shikha</creatorcontrib><creatorcontrib>Laskar, Sarbani Ghosh</creatorcontrib><creatorcontrib>Pai, Prathamesh</creatorcontrib><creatorcontrib>Aggarwal, Jai Prakash</creatorcontrib><creatorcontrib>D'Cruz, Anil</creatorcontrib><creatorcontrib>Chaturvedi, Pankaj</creatorcontrib><creatorcontrib>Deshpande, Mandar</creatorcontrib><creatorcontrib>Chaukar, Devendra</creatorcontrib><creatorcontrib>Budrukkar, Ashwini</creatorcontrib><creatorcontrib>Gupta, Tejpal</creatorcontrib><creatorcontrib>Murthy, Vedang</creatorcontrib><creatorcontrib>Kane, Shubhada</creatorcontrib><creatorcontrib>Thakur, Meenakshi</creatorcontrib><creatorcontrib>Rangarajan, Venkatesh</creatorcontrib><creatorcontrib>Kannan, Sadhana</creatorcontrib><creatorcontrib>Shet, Tanuja</creatorcontrib><creatorcontrib>Kode, Jyoti</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><jtitle>Head & neck</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sengar, Manju</au><au>Chorghe, Siddhesh</au><au>Jadhav, Kamini</au><au>Singh, Shikha</au><au>Laskar, Sarbani Ghosh</au><au>Pai, Prathamesh</au><au>Aggarwal, Jai Prakash</au><au>D'Cruz, Anil</au><au>Chaturvedi, Pankaj</au><au>Deshpande, Mandar</au><au>Chaukar, Devendra</au><au>Budrukkar, Ashwini</au><au>Gupta, Tejpal</au><au>Murthy, Vedang</au><au>Kane, Shubhada</au><au>Thakur, Meenakshi</au><au>Rangarajan, Venkatesh</au><au>Kannan, Sadhana</au><au>Shet, Tanuja</au><au>Kode, Jyoti</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cell-free Epstein-Barr virus-DNA in patients with nasopharyngeal carcinoma: Plasma versus urine</atitle><jtitle>Head & neck</jtitle><addtitle>Head Neck</addtitle><date>2016-04</date><risdate>2016</risdate><volume>38</volume><issue>S1</issue><spage>E1666</spage><epage>E1673</epage><pages>E1666-E1673</pages><issn>1043-3074</issn><eissn>1097-0347</eissn><abstract>ABSTRACT
Background
The purpose of this study was to explore urinary Epstein–Barr virus (EBV)‐DNA as a potential biomarker in patients with nasopharyngeal carcinoma (NPC).
Methods
EBV‐DNA copies were estimated in plasma/urine of patients with NPC (n = 76) by real‐time polymerase chain reaction (PCR) at baseline, during therapy, and at follow‐up. Their correlation with EBV‐RNA expression in tissues (n = 53) was used to assess sensitivity and specificity of plasma/urine EBV‐DNA. Correlation of urine and plasma EBV‐DNA with each other and with radiological response was evaluated.
Results
This study demonstrated that urine EBV‐DNA has high sensitivity (96%) at diagnosis and it correlates well with plasma EBV‐DNA at baseline and after neoadjuvant chemotherapy. The EBV‐DNA copies reduced significantly with therapy (plasma: p < .001; urine: p = .011). Patients with low EBV‐DNA copies demonstrated improved survival (plasma: p = .023; urine: p = .083).
Conclusion
Plasma EBV‐DNA is a good prognostic marker, whereas further study on a larger cohort may help in developing urine EBV‐DNA as a surrogate prognostic marker for patients with NPC. © 2015 Wiley Periodicals, Inc. Head Neck 38: E1666–E1673, 2016</abstract><cop>United States</cop><pub>Blackwell Publishing Ltd</pub><pmid>26667883</pmid><doi>10.1002/hed.24297</doi><tpages>8</tpages></addata></record> |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Adolescent Adult Aged Carcinoma - virology DNA, Viral - blood DNA, Viral - urine Epstein-Barr encoding region in situ hybridization (EBER-ISH) Epstein-Barr virus Epstein-Barr Virus Infections - diagnosis Female Herpesvirus 4, Human Humans Male Middle Aged Nasopharyngeal Carcinoma Nasopharyngeal Neoplasms - virology plasma/urinary Epstein-Barr virus (EBV)-DNA positron emission tomography (PET)-CT Prognosis real-time polymerase chain reaction (PCR) Sensitivity and Specificity Young Adult |
| title | Cell-free Epstein-Barr virus-DNA in patients with nasopharyngeal carcinoma: Plasma versus urine |
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