An association analysis between mitochondrial DNA content, G10398A polymorphism, HPV infection, and the prognosis of cervical cancer in the Chinese Han population
The aim was to analyze quantitative (mitochondrial DNA (mtDNA) content) and qualitative (G10398A polymorphism) mtDNA alterations as well as human papillomavirus (HPV) infection in cervical cancer prognosis. One hundred and twenty-two cases of formalin-fixed paraffin-embedded cervical carcinoma speci...
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| Veröffentlicht in: | Tumor biology 2016-04, Vol.37 (4), p.5599-5607 |
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| creator | Feng, Dali Xu, Hui Li, Xin Wei, Yuehua Jiang, Huangang Xu, Hong Luo, Aihua Zhou, Fuxiang |
| description | The aim was to analyze quantitative (mitochondrial DNA (mtDNA) content) and qualitative (G10398A polymorphism) mtDNA alterations as well as human papillomavirus (HPV) infection in cervical cancer prognosis. One hundred and twenty-two cases of formalin-fixed paraffin-embedded cervical carcinoma specimens were collected from the Yichang Tumor Hospital and Zhongnan Hospital of Wuhan University in the recent 10 years together with medical records. A quantitative real-time PCR (RT-PCR) was used to determine the copy number of the mitochondrial DNA and HPV expression levels. G10398A polymorphism was determined by PCR-RFLP assay. The overall survival of patients with higher mtDNA content was significantly reduced compared with lower mtDNA content patients (
P
= 0.029). But there was no difference of prognosis between the mtDNA 10398 A allele and G allele. However, the Kaplan–Meier survival curve illustrated a significantly reduced overall survival in the patients with 10398A plus high mtDNA copy number compared with the other groups (
P
|
| doi_str_mv | 10.1007/s13277-015-4429-4 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1794494653</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1794494653</sourcerecordid><originalsourceid>FETCH-LOGICAL-c405t-ae7970429d5096e69689c61642dd7f6ad13dc02741052598235c9d3c3601d09d3</originalsourceid><addsrcrecordid>eNqNkc-KFDEQh4Mo7rr6AF4k4MXDtOZ_Osdh1B1hUQ_qtckm6Z0s3UmbdCvzOj6pNTOriCB4SkG--qqoH0JPKXlJCdGvKuVM64ZQ2QjBTCPuoXMqGG8Ib8l9qAkljWAtP0OPar0lABqjHqIzpqTWrZTn6Mc6YVtrdtHOMUOd7LCvseLrMH8PIeExztntcvIl2gG_fr_GLqc5pHmFLynhpl3jKQ_7MZdpF-u4wtuPX3BMfXAH3wqEHs-7gKeSb1I-mHOPXSjfogOfswlq4I_MZhdTqAFvbQLptAzHnR6jB70danhy916gz2_ffNpsm6sPl-8266vGCSLnxgZtNIEzeEmMCsqo1jhFlWDe615ZT7l3hGlBiWTStIxLZzx3XBHqCVQX6MXJC6t-XUKduzFWF4bBppCX2lFthDBCSf4faCu0hEEU0Od_obd5KXDlI8WN0kQroOiJciXXWkLfTSWOtuw7SrpD1t0p6w4i7A5ZdwJ6nt2Zl-sx-N8dv8IFgJ2ACl_pJpQ_Rv_T-hPs97MS</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1783967076</pqid></control><display><type>article</type><title>An association analysis between mitochondrial DNA content, G10398A polymorphism, HPV infection, and the prognosis of cervical cancer in the Chinese Han population</title><source>MEDLINE</source><source>SpringerLink Journals - AutoHoldings</source><creator>Feng, Dali ; Xu, Hui ; Li, Xin ; Wei, Yuehua ; Jiang, Huangang ; Xu, Hong ; Luo, Aihua ; Zhou, Fuxiang</creator><creatorcontrib>Feng, Dali ; Xu, Hui ; Li, Xin ; Wei, Yuehua ; Jiang, Huangang ; Xu, Hong ; Luo, Aihua ; Zhou, Fuxiang</creatorcontrib><description>The aim was to analyze quantitative (mitochondrial DNA (mtDNA) content) and qualitative (G10398A polymorphism) mtDNA alterations as well as human papillomavirus (HPV) infection in cervical cancer prognosis. One hundred and twenty-two cases of formalin-fixed paraffin-embedded cervical carcinoma specimens were collected from the Yichang Tumor Hospital and Zhongnan Hospital of Wuhan University in the recent 10 years together with medical records. A quantitative real-time PCR (RT-PCR) was used to determine the copy number of the mitochondrial DNA and HPV expression levels. G10398A polymorphism was determined by PCR-RFLP assay. The overall survival of patients with higher mtDNA content was significantly reduced compared with lower mtDNA content patients (
P
= 0.029). But there was no difference of prognosis between the mtDNA 10398 A allele and G allele. However, the Kaplan–Meier survival curve illustrated a significantly reduced overall survival in the patients with 10398A plus high mtDNA copy number compared with the other groups (
P
< 0.05). Although no association between HPV expression level and cervical cancer prognosis was observed, 10398A got increased mtDNA content compared with 10398G (
P
< 0.05) and 10398G displayed an increased HPV-positive rate compared with 10398A. Furthermore, HPV-18 and mtDNA content were positively related in the younger subgroup (≤45 years) (correlation coefficient = 0.456,
P
= 0.022). This study indicated that mtDNA content and HPV infection status are associated with cervical cancer prognosis. High mitochondrial DNA content plus 10398 A may be a marker of poor prognosis in cervical cancer. And mtDNA variation may potentially influence the predisposition to HPV infection and cervical carcinogenesis.</description><identifier>ISSN: 1010-4283</identifier><identifier>EISSN: 1423-0380</identifier><identifier>DOI: 10.1007/s13277-015-4429-4</identifier><identifier>PMID: 26577855</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Aged ; Aged, 80 and over ; Alleles ; Biomedical and Life Sciences ; Biomedicine ; Cancer Research ; Cervical cancer ; DNA, Mitochondrial - genetics ; DNA, Mitochondrial - isolation & purification ; Female ; Genotype ; Human papillomavirus ; Human papillomavirus 18 - genetics ; Human papillomavirus 18 - pathogenicity ; Humans ; Kaplan-Meier Estimate ; Medical prognosis ; Middle Aged ; Mitochondrial DNA ; Original Article ; Papillomavirus Infections - genetics ; Papillomavirus Infections - pathology ; Papillomavirus Infections - virology ; Polymorphism ; Polymorphism, Single Nucleotide - genetics ; Prognosis ; Risk Factors ; Uterine Cervical Neoplasms - genetics ; Uterine Cervical Neoplasms - pathology</subject><ispartof>Tumor biology, 2016-04, Vol.37 (4), p.5599-5607</ispartof><rights>International Society of Oncology and BioMarkers (ISOBM) 2015</rights><rights>International Society of Oncology and BioMarkers (ISOBM) 2016</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c405t-ae7970429d5096e69689c61642dd7f6ad13dc02741052598235c9d3c3601d09d3</citedby><cites>FETCH-LOGICAL-c405t-ae7970429d5096e69689c61642dd7f6ad13dc02741052598235c9d3c3601d09d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s13277-015-4429-4$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s13277-015-4429-4$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26577855$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Feng, Dali</creatorcontrib><creatorcontrib>Xu, Hui</creatorcontrib><creatorcontrib>Li, Xin</creatorcontrib><creatorcontrib>Wei, Yuehua</creatorcontrib><creatorcontrib>Jiang, Huangang</creatorcontrib><creatorcontrib>Xu, Hong</creatorcontrib><creatorcontrib>Luo, Aihua</creatorcontrib><creatorcontrib>Zhou, Fuxiang</creatorcontrib><title>An association analysis between mitochondrial DNA content, G10398A polymorphism, HPV infection, and the prognosis of cervical cancer in the Chinese Han population</title><title>Tumor biology</title><addtitle>Tumor Biol</addtitle><addtitle>Tumour Biol</addtitle><description>The aim was to analyze quantitative (mitochondrial DNA (mtDNA) content) and qualitative (G10398A polymorphism) mtDNA alterations as well as human papillomavirus (HPV) infection in cervical cancer prognosis. One hundred and twenty-two cases of formalin-fixed paraffin-embedded cervical carcinoma specimens were collected from the Yichang Tumor Hospital and Zhongnan Hospital of Wuhan University in the recent 10 years together with medical records. A quantitative real-time PCR (RT-PCR) was used to determine the copy number of the mitochondrial DNA and HPV expression levels. G10398A polymorphism was determined by PCR-RFLP assay. The overall survival of patients with higher mtDNA content was significantly reduced compared with lower mtDNA content patients (
P
= 0.029). But there was no difference of prognosis between the mtDNA 10398 A allele and G allele. However, the Kaplan–Meier survival curve illustrated a significantly reduced overall survival in the patients with 10398A plus high mtDNA copy number compared with the other groups (
P
< 0.05). Although no association between HPV expression level and cervical cancer prognosis was observed, 10398A got increased mtDNA content compared with 10398G (
P
< 0.05) and 10398G displayed an increased HPV-positive rate compared with 10398A. Furthermore, HPV-18 and mtDNA content were positively related in the younger subgroup (≤45 years) (correlation coefficient = 0.456,
P
= 0.022). This study indicated that mtDNA content and HPV infection status are associated with cervical cancer prognosis. High mitochondrial DNA content plus 10398 A may be a marker of poor prognosis in cervical cancer. And mtDNA variation may potentially influence the predisposition to HPV infection and cervical carcinogenesis.</description><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Alleles</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Cancer Research</subject><subject>Cervical cancer</subject><subject>DNA, Mitochondrial - genetics</subject><subject>DNA, Mitochondrial - isolation & purification</subject><subject>Female</subject><subject>Genotype</subject><subject>Human papillomavirus</subject><subject>Human papillomavirus 18 - genetics</subject><subject>Human papillomavirus 18 - pathogenicity</subject><subject>Humans</subject><subject>Kaplan-Meier Estimate</subject><subject>Medical prognosis</subject><subject>Middle Aged</subject><subject>Mitochondrial DNA</subject><subject>Original Article</subject><subject>Papillomavirus Infections - genetics</subject><subject>Papillomavirus Infections - pathology</subject><subject>Papillomavirus Infections - virology</subject><subject>Polymorphism</subject><subject>Polymorphism, Single Nucleotide - genetics</subject><subject>Prognosis</subject><subject>Risk Factors</subject><subject>Uterine Cervical Neoplasms - genetics</subject><subject>Uterine Cervical Neoplasms - pathology</subject><issn>1010-4283</issn><issn>1423-0380</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc-KFDEQh4Mo7rr6AF4k4MXDtOZ_Osdh1B1hUQ_qtckm6Z0s3UmbdCvzOj6pNTOriCB4SkG--qqoH0JPKXlJCdGvKuVM64ZQ2QjBTCPuoXMqGG8Ib8l9qAkljWAtP0OPar0lABqjHqIzpqTWrZTn6Mc6YVtrdtHOMUOd7LCvseLrMH8PIeExztntcvIl2gG_fr_GLqc5pHmFLynhpl3jKQ_7MZdpF-u4wtuPX3BMfXAH3wqEHs-7gKeSb1I-mHOPXSjfogOfswlq4I_MZhdTqAFvbQLptAzHnR6jB70danhy916gz2_ffNpsm6sPl-8266vGCSLnxgZtNIEzeEmMCsqo1jhFlWDe615ZT7l3hGlBiWTStIxLZzx3XBHqCVQX6MXJC6t-XUKduzFWF4bBppCX2lFthDBCSf4faCu0hEEU0Od_obd5KXDlI8WN0kQroOiJciXXWkLfTSWOtuw7SrpD1t0p6w4i7A5ZdwJ6nt2Zl-sx-N8dv8IFgJ2ACl_pJpQ_Rv_T-hPs97MS</recordid><startdate>20160401</startdate><enddate>20160401</enddate><creator>Feng, Dali</creator><creator>Xu, Hui</creator><creator>Li, Xin</creator><creator>Wei, Yuehua</creator><creator>Jiang, Huangang</creator><creator>Xu, Hong</creator><creator>Luo, Aihua</creator><creator>Zhou, Fuxiang</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7TO</scope><scope>H94</scope><scope>K9.</scope><scope>7X8</scope><scope>7TM</scope><scope>7U9</scope></search><sort><creationdate>20160401</creationdate><title>An association analysis between mitochondrial DNA content, G10398A polymorphism, HPV infection, and the prognosis of cervical cancer in the Chinese Han population</title><author>Feng, Dali ; Xu, Hui ; Li, Xin ; Wei, Yuehua ; Jiang, Huangang ; Xu, Hong ; Luo, Aihua ; Zhou, Fuxiang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c405t-ae7970429d5096e69689c61642dd7f6ad13dc02741052598235c9d3c3601d09d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Alleles</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Cancer Research</topic><topic>Cervical cancer</topic><topic>DNA, Mitochondrial - genetics</topic><topic>DNA, Mitochondrial - isolation & purification</topic><topic>Female</topic><topic>Genotype</topic><topic>Human papillomavirus</topic><topic>Human papillomavirus 18 - genetics</topic><topic>Human papillomavirus 18 - pathogenicity</topic><topic>Humans</topic><topic>Kaplan-Meier Estimate</topic><topic>Medical prognosis</topic><topic>Middle Aged</topic><topic>Mitochondrial DNA</topic><topic>Original Article</topic><topic>Papillomavirus Infections - genetics</topic><topic>Papillomavirus Infections - pathology</topic><topic>Papillomavirus Infections - virology</topic><topic>Polymorphism</topic><topic>Polymorphism, Single Nucleotide - genetics</topic><topic>Prognosis</topic><topic>Risk Factors</topic><topic>Uterine Cervical Neoplasms - genetics</topic><topic>Uterine Cervical Neoplasms - pathology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Feng, Dali</creatorcontrib><creatorcontrib>Xu, Hui</creatorcontrib><creatorcontrib>Li, Xin</creatorcontrib><creatorcontrib>Wei, Yuehua</creatorcontrib><creatorcontrib>Jiang, Huangang</creatorcontrib><creatorcontrib>Xu, Hong</creatorcontrib><creatorcontrib>Luo, Aihua</creatorcontrib><creatorcontrib>Zhou, Fuxiang</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><jtitle>Tumor biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Feng, Dali</au><au>Xu, Hui</au><au>Li, Xin</au><au>Wei, Yuehua</au><au>Jiang, Huangang</au><au>Xu, Hong</au><au>Luo, Aihua</au><au>Zhou, Fuxiang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>An association analysis between mitochondrial DNA content, G10398A polymorphism, HPV infection, and the prognosis of cervical cancer in the Chinese Han population</atitle><jtitle>Tumor biology</jtitle><stitle>Tumor Biol</stitle><addtitle>Tumour Biol</addtitle><date>2016-04-01</date><risdate>2016</risdate><volume>37</volume><issue>4</issue><spage>5599</spage><epage>5607</epage><pages>5599-5607</pages><issn>1010-4283</issn><eissn>1423-0380</eissn><abstract>The aim was to analyze quantitative (mitochondrial DNA (mtDNA) content) and qualitative (G10398A polymorphism) mtDNA alterations as well as human papillomavirus (HPV) infection in cervical cancer prognosis. One hundred and twenty-two cases of formalin-fixed paraffin-embedded cervical carcinoma specimens were collected from the Yichang Tumor Hospital and Zhongnan Hospital of Wuhan University in the recent 10 years together with medical records. A quantitative real-time PCR (RT-PCR) was used to determine the copy number of the mitochondrial DNA and HPV expression levels. G10398A polymorphism was determined by PCR-RFLP assay. The overall survival of patients with higher mtDNA content was significantly reduced compared with lower mtDNA content patients (
P
= 0.029). But there was no difference of prognosis between the mtDNA 10398 A allele and G allele. However, the Kaplan–Meier survival curve illustrated a significantly reduced overall survival in the patients with 10398A plus high mtDNA copy number compared with the other groups (
P
< 0.05). Although no association between HPV expression level and cervical cancer prognosis was observed, 10398A got increased mtDNA content compared with 10398G (
P
< 0.05) and 10398G displayed an increased HPV-positive rate compared with 10398A. Furthermore, HPV-18 and mtDNA content were positively related in the younger subgroup (≤45 years) (correlation coefficient = 0.456,
P
= 0.022). This study indicated that mtDNA content and HPV infection status are associated with cervical cancer prognosis. High mitochondrial DNA content plus 10398 A may be a marker of poor prognosis in cervical cancer. And mtDNA variation may potentially influence the predisposition to HPV infection and cervical carcinogenesis.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>26577855</pmid><doi>10.1007/s13277-015-4429-4</doi><tpages>9</tpages></addata></record> |
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| subjects | Aged Aged, 80 and over Alleles Biomedical and Life Sciences Biomedicine Cancer Research Cervical cancer DNA, Mitochondrial - genetics DNA, Mitochondrial - isolation & purification Female Genotype Human papillomavirus Human papillomavirus 18 - genetics Human papillomavirus 18 - pathogenicity Humans Kaplan-Meier Estimate Medical prognosis Middle Aged Mitochondrial DNA Original Article Papillomavirus Infections - genetics Papillomavirus Infections - pathology Papillomavirus Infections - virology Polymorphism Polymorphism, Single Nucleotide - genetics Prognosis Risk Factors Uterine Cervical Neoplasms - genetics Uterine Cervical Neoplasms - pathology |
| title | An association analysis between mitochondrial DNA content, G10398A polymorphism, HPV infection, and the prognosis of cervical cancer in the Chinese Han population |
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