An improved tetO promoter replacement system for regulating the expression of yeast genes

Regulatable promoters are commonly used to control the expression of, especially, essential genes in a conditional manner. Integration of such promoters upstream of an ORF using one‐step PCR‐mediated homologous recombination should be particularly efficient. However, integration of the original KanM...

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Veröffentlicht in:	Yeast (Chichester, England) England), 2003-11, Vol.20 (15), p.1255-1262
Hauptverfasser:	Yen, Kuangyu, Gitsham, Paul, Wishart, Jill, Oliver, Stephen G., Zhang, Nianshu
Format:	Artikel
Sprache:	eng
Schlagworte:	Anti-Bacterial Agents - pharmacology Carrier Proteins - genetics Carrier Proteins - metabolism Cell Cycle Proteins - genetics Cell Cycle Proteins - metabolism DNA, Fungal - chemistry DNA, Fungal - genetics DNA-Binding Proteins Doxycycline - pharmacology Gene Expression Regulation, Fungal gene regulation Genes, Essential - genetics Genes, Essential - physiology Mutagenesis, Insertional - methods Nuclear Proteins - genetics Nuclear Proteins - metabolism Phosphoproteins - genetics Phosphoproteins - metabolism Promoter Regions, Genetic - genetics Promoter Regions, Genetic - physiology promoter replacement Saccharomyces cerevisiae Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae - metabolism Saccharomyces cerevisiae Proteins - genetics Saccharomyces cerevisiae Proteins - metabolism Securin tetO promoter Trans-Activators - genetics Trans-Activators - metabolism Transformation, Genetic - genetics
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