Real-time reverse transcription PCR analysis of expression of atrazine catabolism genes in two bacterial strains isolated from soil

The level of expression of highly conserved, plasmid-borne, and widely dispersed atrazine catabolic genes ( atz) was studied by RT-qPCR in two telluric atrazine-degrading microbes. RT-qPCR assays, based on the use of real-time PCR, were developed in order to quantify atzABCDEF mRNAs in Pseudomonas s...

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Veröffentlicht in:	Journal of microbiological methods 2004, Vol.56 (1), p.3-15
Hauptverfasser:	Devers, Marion, Soulas, Guy, Martin-Laurent, Fabrice
Format:	Artikel
Sprache:	eng
Schlagworte:	Atrazine Atrazine - metabolism atz gene Biodegradation Biodegradation, Environmental Biological and medical sciences Chelatobacter heintzii Fundamental and applied biological sciences. Psychology Gene expression Gene Expression Regulation, Bacterial Herbicides - metabolism Kinetics Microbiology Proteobacteria - genetics Proteobacteria - metabolism Pseudomonas Pseudomonas - genetics Pseudomonas - metabolism Reverse Transcriptase Polymerase Chain Reaction - methods RNA, Messenger - biosynthesis RNA, Messenger - genetics RNA, Ribosomal, 16S - genetics RT-qPCR Soil Microbiology Soil Pollutants - metabolism
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