Selection of biochemical mutants of Aspergillus niger with enhanced extracellular ribonuclease production

Combined with u.v. irradiation and the nitrosoguanidine method, selection of biochemical mutants resistant to metabolic inhibitors (2-deoxy-D-glucose, antimycin A, sodium orthovanadate and sodium azide) was a very efficient method for improvement of ribonuclease production by Aspergillus niger. Resi...

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Veröffentlicht in:World journal of microbiology & biotechnology 2004-03, Vol.20 (2), p.203-206
Hauptverfasser: XIONG, Ya-Hong, LIU, Jian-Zhong, SONG, Hai-Yan, WENG, Li-Ping, JI, Liang-Nian
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container_title World journal of microbiology & biotechnology
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creator XIONG, Ya-Hong
LIU, Jian-Zhong
SONG, Hai-Yan
WENG, Li-Ping
JI, Liang-Nian
description Combined with u.v. irradiation and the nitrosoguanidine method, selection of biochemical mutants resistant to metabolic inhibitors (2-deoxy-D-glucose, antimycin A, sodium orthovanadate and sodium azide) was a very efficient method for improvement of ribonuclease production by Aspergillus niger. Resistance to sodium azide produced highest RNase production, greatest frequency of positive mutation and shortest sporulation time. The most active strain, Aspergillus niger SA-13-20 resistant to sodium azide, was obtained, which had a 433% increase in RNase production in comparison with the parent strain and had good subculturing stability. Its dynamic characters were similar to those of the parent strain.[PUBLICATION ABSTRACT]
doi_str_mv 10.1023/B:WIBI.0000021782.84314.53
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source SpringerNature Journals
subjects Aspergillus niger
Biological and medical sciences
Biotechnology
Eukaryotes
Food science
Fundamental and applied biological sciences. Psychology
Genetic technics
Irradiation
Methods. Procedures. Technologies
Mutant screening
Sodium
Ultraviolet radiation
title Selection of biochemical mutants of Aspergillus niger with enhanced extracellular ribonuclease production
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