Selection of biochemical mutants of Aspergillus niger with enhanced extracellular ribonuclease production
Combined with u.v. irradiation and the nitrosoguanidine method, selection of biochemical mutants resistant to metabolic inhibitors (2-deoxy-D-glucose, antimycin A, sodium orthovanadate and sodium azide) was a very efficient method for improvement of ribonuclease production by Aspergillus niger. Resi...
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Veröffentlicht in: | World journal of microbiology & biotechnology 2004-03, Vol.20 (2), p.203-206 |
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creator | XIONG, Ya-Hong LIU, Jian-Zhong SONG, Hai-Yan WENG, Li-Ping JI, Liang-Nian |
description | Combined with u.v. irradiation and the nitrosoguanidine method, selection of biochemical mutants resistant to metabolic inhibitors (2-deoxy-D-glucose, antimycin A, sodium orthovanadate and sodium azide) was a very efficient method for improvement of ribonuclease production by Aspergillus niger. Resistance to sodium azide produced highest RNase production, greatest frequency of positive mutation and shortest sporulation time. The most active strain, Aspergillus niger SA-13-20 resistant to sodium azide, was obtained, which had a 433% increase in RNase production in comparison with the parent strain and had good subculturing stability. Its dynamic characters were similar to those of the parent strain.[PUBLICATION ABSTRACT] |
doi_str_mv | 10.1023/B:WIBI.0000021782.84314.53 |
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Resistance to sodium azide produced highest RNase production, greatest frequency of positive mutation and shortest sporulation time. The most active strain, Aspergillus niger SA-13-20 resistant to sodium azide, was obtained, which had a 433% increase in RNase production in comparison with the parent strain and had good subculturing stability. Its dynamic characters were similar to those of the parent strain.[PUBLICATION ABSTRACT]</description><identifier>ISSN: 0959-3993</identifier><identifier>EISSN: 1573-0972</identifier><identifier>DOI: 10.1023/B:WIBI.0000021782.84314.53</identifier><language>eng</language><publisher>Dordrecht: Springer</publisher><subject>Aspergillus niger ; Biological and medical sciences ; Biotechnology ; Eukaryotes ; Food science ; Fundamental and applied biological sciences. Psychology ; Genetic technics ; Irradiation ; Methods. Procedures. 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Resistance to sodium azide produced highest RNase production, greatest frequency of positive mutation and shortest sporulation time. The most active strain, Aspergillus niger SA-13-20 resistant to sodium azide, was obtained, which had a 433% increase in RNase production in comparison with the parent strain and had good subculturing stability. Its dynamic characters were similar to those of the parent strain.[PUBLICATION ABSTRACT]</description><subject>Aspergillus niger</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Eukaryotes</subject><subject>Food science</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetic technics</subject><subject>Irradiation</subject><subject>Methods. Procedures. 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subjects | Aspergillus niger Biological and medical sciences Biotechnology Eukaryotes Food science Fundamental and applied biological sciences. Psychology Genetic technics Irradiation Methods. Procedures. Technologies Mutant screening Sodium Ultraviolet radiation |
title | Selection of biochemical mutants of Aspergillus niger with enhanced extracellular ribonuclease production |
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