Ion-induced fluorescence imaging of endosomes
Imaging laboratories at Jyväskylä and Singapore are collaborating on the development of fluorescence imaging of cytoplasmic endosomes using a combination of proton induced fluorescence (PIF) with direct Scanning Transmission Ion Microscopy (direct-STIM) for sub-cellular structural imaging. A549 lung...
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| Veröffentlicht in: | Nuclear instruments & methods in physics research. Section B, Beam interactions with materials and atoms Beam interactions with materials and atoms, 2013-07, Vol.306, p.113-116 |
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| container_title | Nuclear instruments & methods in physics research. Section B, Beam interactions with materials and atoms |
| container_volume | 306 |
| creator | Norarat, R. Marjomäki, V. Chen, X. Zhaohong, M. Minqin, R. Chen, C.-B. Bettiol, A.A. Whitlow, H.J. Watt, F. |
| description | Imaging laboratories at Jyväskylä and Singapore are collaborating on the development of fluorescence imaging of cytoplasmic endosomes using a combination of proton induced fluorescence (PIF) with direct Scanning Transmission Ion Microscopy (direct-STIM) for sub-cellular structural imaging. A549 lung carcinoma cells were cultivated and stained for epidermal growth factor receptor (EGFR) and receptor α2β1 integrin. In this paper, we demonstrate that cells can be imaged at sub-150nm resolution using the PIF technique. In addition, the same target cell was imaged at 50 and 25nm resolution by using proton and He-STIM, respectively. The combination of both techniques offer a powerful tool to improve fluorescence imaging beyond optical diffraction limits. |
| doi_str_mv | 10.1016/j.nimb.2012.12.052 |
| format | Article |
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The combination of both techniques offer a powerful tool to improve fluorescence imaging beyond optical diffraction limits.</description><subject>Autofluorescence</subject><subject>Diffraction</subject><subject>Diffraction limit</subject><subject>Endosomes</subject><subject>Fluorescence</subject><subject>Imaging</subject><subject>Laboratories</subject><subject>Microscopy</subject><subject>Proton-induced fluorescence (PIF)</subject><subject>Receptors</subject><subject>Scanning</subject><subject>Scanning Transmission Ion Microscopy (STIM)</subject><issn>0168-583X</issn><issn>1872-9584</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><recordid>eNp9kM1LxDAQxYMouK7-A5569NLafLRJwIssfiwseFHwFtrJdMnSNmuyFfzvTalnh4G5vDe89yPklpYFLWl9fyhGN7QFKykr0pYVOyMrqiTLdaXEOVklkcorxT8vyVWMhzJNxasVybd-zN1oJ0Cbdf3kA0bAETBzQ7N34z7zXYaj9dEPGK_JRdf0EW_-7pp8PD-9b17z3dvLdvO4y4FzfsptxQBRtxQtyFYzLoDJGhqoWyVbKUUKJWmnGOgSWuiE5oJpVdd103DZcb4md8vfY_BfE8aTGVyK1ffNiH6KhkrNmRZKiiRlixSCjzFgZ44hRQ8_hpZmZmMOZmZjZjYmbWKTTA-LCVOJb4fBRHBza-sCwslY7_6z_wJux2x0</recordid><startdate>20130701</startdate><enddate>20130701</enddate><creator>Norarat, R.</creator><creator>Marjomäki, V.</creator><creator>Chen, X.</creator><creator>Zhaohong, M.</creator><creator>Minqin, R.</creator><creator>Chen, C.-B.</creator><creator>Bettiol, A.A.</creator><creator>Whitlow, H.J.</creator><creator>Watt, F.</creator><general>Elsevier B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7U5</scope><scope>8FD</scope><scope>L7M</scope></search><sort><creationdate>20130701</creationdate><title>Ion-induced fluorescence imaging of endosomes</title><author>Norarat, R. ; Marjomäki, V. ; Chen, X. ; Zhaohong, M. ; Minqin, R. ; Chen, C.-B. ; Bettiol, A.A. ; Whitlow, H.J. ; Watt, F.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c333t-d52cee9b1edc7b9234c276cac6b87b77495871f82c90cbcf4934298666aa37f33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Autofluorescence</topic><topic>Diffraction</topic><topic>Diffraction limit</topic><topic>Endosomes</topic><topic>Fluorescence</topic><topic>Imaging</topic><topic>Laboratories</topic><topic>Microscopy</topic><topic>Proton-induced fluorescence (PIF)</topic><topic>Receptors</topic><topic>Scanning</topic><topic>Scanning Transmission Ion Microscopy (STIM)</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Norarat, R.</creatorcontrib><creatorcontrib>Marjomäki, V.</creatorcontrib><creatorcontrib>Chen, X.</creatorcontrib><creatorcontrib>Zhaohong, M.</creatorcontrib><creatorcontrib>Minqin, R.</creatorcontrib><creatorcontrib>Chen, C.-B.</creatorcontrib><creatorcontrib>Bettiol, A.A.</creatorcontrib><creatorcontrib>Whitlow, H.J.</creatorcontrib><creatorcontrib>Watt, F.</creatorcontrib><collection>CrossRef</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Technology Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><jtitle>Nuclear instruments & methods in physics research. 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| identifier | ISSN: 0168-583X |
| ispartof | Nuclear instruments & methods in physics research. Section B, Beam interactions with materials and atoms, 2013-07, Vol.306, p.113-116 |
| issn | 0168-583X 1872-9584 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1793294874 |
| source | ScienceDirect Journals (5 years ago - present) |
| subjects | Autofluorescence Diffraction Diffraction limit Endosomes Fluorescence Imaging Laboratories Microscopy Proton-induced fluorescence (PIF) Receptors Scanning Scanning Transmission Ion Microscopy (STIM) |
| title | Ion-induced fluorescence imaging of endosomes |
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