Electrochemical determination of microRNAs based on isothermal strand-displacement polymerase reaction coupled with multienzyme functionalized magnetic micro-carriers

MicroRNAs (miRNAs) show great potential for disease diagnostics due to their specific molecular profiles. Detection of miRNAs remains challenging and often requires sophisticated platforms. Here we report a multienzyme-functionalized magnetic microcarriers-assisted isothermal strand-displacement pol...

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Veröffentlicht in:	Biosensors & bioelectronics 2016-06, Vol.80, p.344-351
Hauptverfasser:	Ma, Wen, Situ, Bo, Lv, Weifeng, Li, Bo, Yin, Xiaomao, Vadgama, Pankaj, Zheng, Lei, Wang, Wen
Format:	Artikel
Sprache:	eng
Schlagworte:	Biosensing Techniques - methods Biosensors Biotin - chemistry Breast - pathology Breast Neoplasms - diagnosis Breast Neoplasms - genetics Carbon Diagnostic systems Electrochemical sensing assay Electrochemical Techniques - methods Electrodes Female Humans Limit of Detection Magnetic microcarriers Magnets - chemistry Metal matrix composites MicroRNA MicroRNAs - analysis Nanoparticles - chemistry Nanoparticles - ultrastructure Nucleic Acid Amplification Techniques - methods Polymerase Recognition Ribonucleic acids Sensitivity and Specificity Signal amplification Streptavidin - chemistry
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