Kinesin-8 motors improve nuclear centering by promoting microtubule catastrophe
In fission yeast, microtubules push against the cell edge, thereby positioning the nucleus in the cell center. Kinesin-8 motors regulate microtubule catastrophe; however, their role in nuclear positioning is not known. Here we develop a physical model that describes how kinesin-8 motors affect nucle...
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| Veröffentlicht in: | Physical review letters 2015-02, Vol.114 (7), p.078103-078103, Article 078103 |
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| container_end_page | 078103 |
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| container_issue | 7 |
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| container_title | Physical review letters |
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| creator | Glunčić, Matko Maghelli, Nicola Krull, Alexander Krstić, Vladimir Ramunno-Johnson, Damien Pavin, Nenad Tolić, Iva M |
| description | In fission yeast, microtubules push against the cell edge, thereby positioning the nucleus in the cell center. Kinesin-8 motors regulate microtubule catastrophe; however, their role in nuclear positioning is not known. Here we develop a physical model that describes how kinesin-8 motors affect nuclear centering by promoting a microtubule catastrophe. Our model predicts the improved centering of the nucleus in the presence of motors, which we confirmed experimentally in living cells. The model also predicts a characteristic time for the recentering of a displaced nucleus, which is supported by our experiments where we displaced the nucleus using optical tweezers. |
| doi_str_mv | 10.1103/PhysRevLett.114.078103 |
| format | Article |
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The model also predicts a characteristic time for the recentering of a displaced nucleus, which is supported by our experiments where we displaced the nucleus using optical tweezers.</description><identifier>ISSN: 0031-9007</identifier><identifier>EISSN: 1079-7114</identifier><identifier>DOI: 10.1103/PhysRevLett.114.078103</identifier><identifier>PMID: 25763975</identifier><language>eng</language><publisher>United States</publisher><subject>Cell Nucleus - physiology ; Cells (biology) ; Displacement ; Kinesin - physiology ; Mathematical models ; Microtubules - physiology ; Models, Biological ; Motors ; Nuclear fission ; Nuclei ; Optical Tweezers ; Positioning ; Schizosaccharomyces - physiology ; Yeast</subject><ispartof>Physical review letters, 2015-02, Vol.114 (7), p.078103-078103, Article 078103</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c392t-90669ec50abcf730da69ef3cba11c7539f5fe7f4da24cf18144ae3bcef1cb19d3</citedby><cites>FETCH-LOGICAL-c392t-90669ec50abcf730da69ef3cba11c7539f5fe7f4da24cf18144ae3bcef1cb19d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,2862,2863,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25763975$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Glunčić, Matko</creatorcontrib><creatorcontrib>Maghelli, Nicola</creatorcontrib><creatorcontrib>Krull, Alexander</creatorcontrib><creatorcontrib>Krstić, Vladimir</creatorcontrib><creatorcontrib>Ramunno-Johnson, Damien</creatorcontrib><creatorcontrib>Pavin, Nenad</creatorcontrib><creatorcontrib>Tolić, Iva M</creatorcontrib><title>Kinesin-8 motors improve nuclear centering by promoting microtubule catastrophe</title><title>Physical review letters</title><addtitle>Phys Rev Lett</addtitle><description>In fission yeast, microtubules push against the cell edge, thereby positioning the nucleus in the cell center. Kinesin-8 motors regulate microtubule catastrophe; however, their role in nuclear positioning is not known. Here we develop a physical model that describes how kinesin-8 motors affect nuclear centering by promoting a microtubule catastrophe. Our model predicts the improved centering of the nucleus in the presence of motors, which we confirmed experimentally in living cells. The model also predicts a characteristic time for the recentering of a displaced nucleus, which is supported by our experiments where we displaced the nucleus using optical tweezers.</description><subject>Cell Nucleus - physiology</subject><subject>Cells (biology)</subject><subject>Displacement</subject><subject>Kinesin - physiology</subject><subject>Mathematical models</subject><subject>Microtubules - physiology</subject><subject>Models, Biological</subject><subject>Motors</subject><subject>Nuclear fission</subject><subject>Nuclei</subject><subject>Optical Tweezers</subject><subject>Positioning</subject><subject>Schizosaccharomyces - physiology</subject><subject>Yeast</subject><issn>0031-9007</issn><issn>1079-7114</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFUMtOwzAQtBCIlsIvVDlySfHGcRwfUcVLVCpCcI4cZ02D8ii2Uyl_j6sWxI3TamZnd2eHkDnQBQBlNy-b0b3iboXeByJdUJEH-oRMgQoZi0CdkimlDGJJqZiQC-c-KaWQZPk5mSRcZEwKPiXr57pDV3dxHrW9762L6nZr-x1G3aAbVDbS2Hm0dfcRlWMUWkG2B22tbe-Hcmgw0sor522_3eAlOTOqcXh1rDPyfn_3tnyMV-uHp-XtKtZMJj6YyjKJmlNVaiMYrVSAhulSAWjBmTTcoDBppZJUG8ghTRWyUqMBXYKs2IxcH_YGR18DOl-0tdPYNKrDfnAFCMkSnic0_1-aZWkCjPO9NDtIw2_OWTTF1tatsmMBtNjnXvzJPRBpccg9DM6PN4ayxep37Cdo9g139oOR</recordid><startdate>20150220</startdate><enddate>20150220</enddate><creator>Glunčić, Matko</creator><creator>Maghelli, Nicola</creator><creator>Krull, Alexander</creator><creator>Krstić, Vladimir</creator><creator>Ramunno-Johnson, Damien</creator><creator>Pavin, Nenad</creator><creator>Tolić, Iva M</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7U5</scope><scope>8FD</scope><scope>H8D</scope><scope>L7M</scope></search><sort><creationdate>20150220</creationdate><title>Kinesin-8 motors improve nuclear centering by promoting microtubule catastrophe</title><author>Glunčić, Matko ; Maghelli, Nicola ; Krull, Alexander ; Krstić, Vladimir ; Ramunno-Johnson, Damien ; Pavin, Nenad ; Tolić, Iva M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c392t-90669ec50abcf730da69ef3cba11c7539f5fe7f4da24cf18144ae3bcef1cb19d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Cell Nucleus - physiology</topic><topic>Cells (biology)</topic><topic>Displacement</topic><topic>Kinesin - physiology</topic><topic>Mathematical models</topic><topic>Microtubules - physiology</topic><topic>Models, Biological</topic><topic>Motors</topic><topic>Nuclear fission</topic><topic>Nuclei</topic><topic>Optical Tweezers</topic><topic>Positioning</topic><topic>Schizosaccharomyces - physiology</topic><topic>Yeast</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Glunčić, Matko</creatorcontrib><creatorcontrib>Maghelli, Nicola</creatorcontrib><creatorcontrib>Krull, Alexander</creatorcontrib><creatorcontrib>Krstić, Vladimir</creatorcontrib><creatorcontrib>Ramunno-Johnson, Damien</creatorcontrib><creatorcontrib>Pavin, Nenad</creatorcontrib><creatorcontrib>Tolić, Iva M</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Technology Research Database</collection><collection>Aerospace Database</collection><collection>Advanced Technologies Database with Aerospace</collection><jtitle>Physical review letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Glunčić, Matko</au><au>Maghelli, Nicola</au><au>Krull, Alexander</au><au>Krstić, Vladimir</au><au>Ramunno-Johnson, Damien</au><au>Pavin, Nenad</au><au>Tolić, Iva M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Kinesin-8 motors improve nuclear centering by promoting microtubule catastrophe</atitle><jtitle>Physical review letters</jtitle><addtitle>Phys Rev Lett</addtitle><date>2015-02-20</date><risdate>2015</risdate><volume>114</volume><issue>7</issue><spage>078103</spage><epage>078103</epage><pages>078103-078103</pages><artnum>078103</artnum><issn>0031-9007</issn><eissn>1079-7114</eissn><abstract>In fission yeast, microtubules push against the cell edge, thereby positioning the nucleus in the cell center. 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| ispartof | Physical review letters, 2015-02, Vol.114 (7), p.078103-078103, Article 078103 |
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| language | eng |
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| source | MEDLINE; American Physical Society Journals |
| subjects | Cell Nucleus - physiology Cells (biology) Displacement Kinesin - physiology Mathematical models Microtubules - physiology Models, Biological Motors Nuclear fission Nuclei Optical Tweezers Positioning Schizosaccharomyces - physiology Yeast |
| title | Kinesin-8 motors improve nuclear centering by promoting microtubule catastrophe |
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