Preparation and evaluation of polyethylenimine-functionalized carbon nanotubes tagged with 5TR1 aptamer for targeted delivery of Bcl-xL shRNA into breast cancer cells
Schematic illustration of targeted shRNA delivery using aptamer-conjugated polyplexes. [Display omitted] •Eight types of nanoparticles were synthesized by covalent attachment of SWCNT to PEG and PEI 10kDa or its derivatives•Fabricated nanoparticles showed better transfection efficiency and less cyto...
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creator | Taghavi, Sahar HashemNia, Azadeh Mosaffa, Fatemeh Askarian, Saeedeh Abnous, Khalil Ramezani, Mohammad |
description | Schematic illustration of targeted shRNA delivery using aptamer-conjugated polyplexes.
[Display omitted]
•Eight types of nanoparticles were synthesized by covalent attachment of SWCNT to PEG and PEI 10kDa or its derivatives•Fabricated nanoparticles showed better transfection efficiency and less cytotoxicity as compared with unmodified PEI 10kDa.•5TR1 Apt-conjugated nanoparticles efficiently delivered plasmid Bcl-xL shRNA to breast cancer cell line (MCF7).•Our results demonstrated the potential and specificity of functionalized Apt-carbon nanotubes conjugates for increasing the induction of apoptosis in tumor cells by suppression of Bcl-xL transcript.
In this study, single-walled carbon nanotubes (SWCNTs) were covalently attached to poly(ethylene glycol) (PEG) and polyethylenimine (PEI) 10kDa, or its derivatives, to fabricate efficient carriers for gene delivery. PEI 10kDa was modified by alkylcarboxylation of its primary amines with a series of ω-bromo-alkylcarboxylic acids to provide a range of vectors with increased lipophilicity. PEI 10kDa or its alkylcarboxylate derivatives were conjugated to SWCNT-PEG to develop vectors possessing effective DNA condensation ability which can interact with cell membrane via both nano-needle mechanism and electrostatic interactions produced by SWCNT and PEI, respectively. The results demonstrated that SWCNT-PEG-PEI and SWCNT-PEG-derivatives of PEI could condense DNA into particle size less than 150nm with positive surface charges between 6.3–30.8mV. To improve the antitumor efficacy, we developed a targeted gene delivery system using a 5TR1 aptamer. The most efficient vector, which was prepared by attachment of SWCNT-PEG to modified PEI 10kDa with 10-bromodecanoic acid (10%), showed 8.5–10 folds enhancement in transfection activity at C/P ratio 6 as compared to the gold standard PEI 25kDa at C/P ratio of 0.8. We also showed that the selected polyplex could efficiently and selectively transfer plasmid shRNA to MUC1 positive cells. |
doi_str_mv | 10.1016/j.colsurfb.2015.12.021 |
format | Article |
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[Display omitted]
•Eight types of nanoparticles were synthesized by covalent attachment of SWCNT to PEG and PEI 10kDa or its derivatives•Fabricated nanoparticles showed better transfection efficiency and less cytotoxicity as compared with unmodified PEI 10kDa.•5TR1 Apt-conjugated nanoparticles efficiently delivered plasmid Bcl-xL shRNA to breast cancer cell line (MCF7).•Our results demonstrated the potential and specificity of functionalized Apt-carbon nanotubes conjugates for increasing the induction of apoptosis in tumor cells by suppression of Bcl-xL transcript.
In this study, single-walled carbon nanotubes (SWCNTs) were covalently attached to poly(ethylene glycol) (PEG) and polyethylenimine (PEI) 10kDa, or its derivatives, to fabricate efficient carriers for gene delivery. PEI 10kDa was modified by alkylcarboxylation of its primary amines with a series of ω-bromo-alkylcarboxylic acids to provide a range of vectors with increased lipophilicity. PEI 10kDa or its alkylcarboxylate derivatives were conjugated to SWCNT-PEG to develop vectors possessing effective DNA condensation ability which can interact with cell membrane via both nano-needle mechanism and electrostatic interactions produced by SWCNT and PEI, respectively. The results demonstrated that SWCNT-PEG-PEI and SWCNT-PEG-derivatives of PEI could condense DNA into particle size less than 150nm with positive surface charges between 6.3–30.8mV. To improve the antitumor efficacy, we developed a targeted gene delivery system using a 5TR1 aptamer. The most efficient vector, which was prepared by attachment of SWCNT-PEG to modified PEI 10kDa with 10-bromodecanoic acid (10%), showed 8.5–10 folds enhancement in transfection activity at C/P ratio 6 as compared to the gold standard PEI 25kDa at C/P ratio of 0.8. We also showed that the selected polyplex could efficiently and selectively transfer plasmid shRNA to MUC1 positive cells.</description><identifier>ISSN: 0927-7765</identifier><identifier>EISSN: 1873-4367</identifier><identifier>DOI: 10.1016/j.colsurfb.2015.12.021</identifier><identifier>PMID: 26731195</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Aptamer ; Aptamers, Nucleotide - chemistry ; Aptamers, Nucleotide - genetics ; bcl-X Protein - genetics ; bcl-X Protein - metabolism ; Bcl-xL shRNA ; Blotting, Western ; Cell Line, Tumor ; Cell Survival - genetics ; Condensing ; Deoxyribonucleic acid ; Derivatives ; Gene Transfer Techniques ; Genes ; Humans ; Mathematical analysis ; MCF-7 Cells ; Microscopy, Atomic Force ; Mucin-1 - genetics ; Mucin-1 - metabolism ; Nanotubes, Carbon - chemistry ; Particle Size ; Polyetherimides ; Polyethylene Glycols - chemistry ; Polyethyleneimine - chemistry ; Polyethylenimine ; RNA, Small Interfering - chemistry ; RNA, Small Interfering - genetics ; SELEX Aptamer Technique ; Single wall carbon nanotubes ; Single-walled carbon nanotubes ; Targeted gene delivery ; Transfection - methods ; Vectors (mathematics)</subject><ispartof>Colloids and surfaces, B, Biointerfaces, 2016-04, Vol.140, p.28-39</ispartof><rights>2015 Elsevier B.V.</rights><rights>Copyright © 2015 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c471t-ac19e54878e93342035396382ef55331ceae491eaaf6559b8645b670add7ac43</citedby><cites>FETCH-LOGICAL-c471t-ac19e54878e93342035396382ef55331ceae491eaaf6559b8645b670add7ac43</cites><orcidid>0000-0001-6314-0164</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0927776515303696$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,65309</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26731195$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Taghavi, Sahar</creatorcontrib><creatorcontrib>HashemNia, Azadeh</creatorcontrib><creatorcontrib>Mosaffa, Fatemeh</creatorcontrib><creatorcontrib>Askarian, Saeedeh</creatorcontrib><creatorcontrib>Abnous, Khalil</creatorcontrib><creatorcontrib>Ramezani, Mohammad</creatorcontrib><title>Preparation and evaluation of polyethylenimine-functionalized carbon nanotubes tagged with 5TR1 aptamer for targeted delivery of Bcl-xL shRNA into breast cancer cells</title><title>Colloids and surfaces, B, Biointerfaces</title><addtitle>Colloids Surf B Biointerfaces</addtitle><description>Schematic illustration of targeted shRNA delivery using aptamer-conjugated polyplexes.
[Display omitted]
•Eight types of nanoparticles were synthesized by covalent attachment of SWCNT to PEG and PEI 10kDa or its derivatives•Fabricated nanoparticles showed better transfection efficiency and less cytotoxicity as compared with unmodified PEI 10kDa.•5TR1 Apt-conjugated nanoparticles efficiently delivered plasmid Bcl-xL shRNA to breast cancer cell line (MCF7).•Our results demonstrated the potential and specificity of functionalized Apt-carbon nanotubes conjugates for increasing the induction of apoptosis in tumor cells by suppression of Bcl-xL transcript.
In this study, single-walled carbon nanotubes (SWCNTs) were covalently attached to poly(ethylene glycol) (PEG) and polyethylenimine (PEI) 10kDa, or its derivatives, to fabricate efficient carriers for gene delivery. PEI 10kDa was modified by alkylcarboxylation of its primary amines with a series of ω-bromo-alkylcarboxylic acids to provide a range of vectors with increased lipophilicity. PEI 10kDa or its alkylcarboxylate derivatives were conjugated to SWCNT-PEG to develop vectors possessing effective DNA condensation ability which can interact with cell membrane via both nano-needle mechanism and electrostatic interactions produced by SWCNT and PEI, respectively. The results demonstrated that SWCNT-PEG-PEI and SWCNT-PEG-derivatives of PEI could condense DNA into particle size less than 150nm with positive surface charges between 6.3–30.8mV. To improve the antitumor efficacy, we developed a targeted gene delivery system using a 5TR1 aptamer. The most efficient vector, which was prepared by attachment of SWCNT-PEG to modified PEI 10kDa with 10-bromodecanoic acid (10%), showed 8.5–10 folds enhancement in transfection activity at C/P ratio 6 as compared to the gold standard PEI 25kDa at C/P ratio of 0.8. We also showed that the selected polyplex could efficiently and selectively transfer plasmid shRNA to MUC1 positive cells.</description><subject>Aptamer</subject><subject>Aptamers, Nucleotide - chemistry</subject><subject>Aptamers, Nucleotide - genetics</subject><subject>bcl-X Protein - genetics</subject><subject>bcl-X Protein - metabolism</subject><subject>Bcl-xL shRNA</subject><subject>Blotting, Western</subject><subject>Cell Line, Tumor</subject><subject>Cell Survival - genetics</subject><subject>Condensing</subject><subject>Deoxyribonucleic acid</subject><subject>Derivatives</subject><subject>Gene Transfer Techniques</subject><subject>Genes</subject><subject>Humans</subject><subject>Mathematical analysis</subject><subject>MCF-7 Cells</subject><subject>Microscopy, Atomic Force</subject><subject>Mucin-1 - genetics</subject><subject>Mucin-1 - metabolism</subject><subject>Nanotubes, Carbon - chemistry</subject><subject>Particle Size</subject><subject>Polyetherimides</subject><subject>Polyethylene Glycols - chemistry</subject><subject>Polyethyleneimine - chemistry</subject><subject>Polyethylenimine</subject><subject>RNA, Small Interfering - chemistry</subject><subject>RNA, Small Interfering - genetics</subject><subject>SELEX Aptamer Technique</subject><subject>Single wall carbon nanotubes</subject><subject>Single-walled carbon nanotubes</subject><subject>Targeted gene delivery</subject><subject>Transfection - methods</subject><subject>Vectors (mathematics)</subject><issn>0927-7765</issn><issn>1873-4367</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkcFu1DAQhiMEokvhFSofuSR47DhObpQKCtIKULV3y3Emu14l8WI7W5YH6nPiaFuu5WSN5_v_Gc2fZVdAC6BQfdgXxg1h9n1bMAqiAFZQBi-yFdSS5yWv5MtsRRsmcykrcZG9CWFPKWUlyNfZBaskB2jEKnv46fGgvY7WTURPHcGjHuZz6XpycMMJ4-404GRHO2Hez5NZmnqwf7AjRvs2kZOeXJxbDCTq7Tb939u4I2JzB0Qfoh7Rk9751PRbjKnd4WCP6E_LiE9myH-vSdjdfb8mdoqOtB51iMl7MklocBjC2-xVr4eA7x7fy2zz5fPm5mu-_nH77eZ6nZtSQsy1gQZFWcsaG85LRrngTcVrhr0QnINBjWUDqHVfCdG0dVWKtpJUd53UpuSX2fuz7cG7XzOGqEYblgX0hG4OCmTDmQCezJ9Hq1rIsvw_VACwtG9CqzNqvAvBY68O3o7anxRQtQSv9uopeLUEr4CpFHwSXj3OmNsRu3-yp6QT8PEMYDrf0aJXwVhMF-6sRxNV5-xzM_4Cd2LExg</recordid><startdate>20160401</startdate><enddate>20160401</enddate><creator>Taghavi, Sahar</creator><creator>HashemNia, Azadeh</creator><creator>Mosaffa, Fatemeh</creator><creator>Askarian, Saeedeh</creator><creator>Abnous, Khalil</creator><creator>Ramezani, Mohammad</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7SR</scope><scope>7U5</scope><scope>8BQ</scope><scope>JG9</scope><scope>L7M</scope><orcidid>https://orcid.org/0000-0001-6314-0164</orcidid></search><sort><creationdate>20160401</creationdate><title>Preparation and evaluation of polyethylenimine-functionalized carbon nanotubes tagged with 5TR1 aptamer for targeted delivery of Bcl-xL shRNA into breast cancer cells</title><author>Taghavi, Sahar ; HashemNia, Azadeh ; Mosaffa, Fatemeh ; Askarian, Saeedeh ; Abnous, Khalil ; Ramezani, Mohammad</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c471t-ac19e54878e93342035396382ef55331ceae491eaaf6559b8645b670add7ac43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Aptamer</topic><topic>Aptamers, Nucleotide - chemistry</topic><topic>Aptamers, Nucleotide - genetics</topic><topic>bcl-X Protein - genetics</topic><topic>bcl-X Protein - metabolism</topic><topic>Bcl-xL shRNA</topic><topic>Blotting, Western</topic><topic>Cell Line, Tumor</topic><topic>Cell Survival - genetics</topic><topic>Condensing</topic><topic>Deoxyribonucleic acid</topic><topic>Derivatives</topic><topic>Gene Transfer Techniques</topic><topic>Genes</topic><topic>Humans</topic><topic>Mathematical analysis</topic><topic>MCF-7 Cells</topic><topic>Microscopy, Atomic Force</topic><topic>Mucin-1 - genetics</topic><topic>Mucin-1 - metabolism</topic><topic>Nanotubes, Carbon - chemistry</topic><topic>Particle Size</topic><topic>Polyetherimides</topic><topic>Polyethylene Glycols - chemistry</topic><topic>Polyethyleneimine - chemistry</topic><topic>Polyethylenimine</topic><topic>RNA, Small Interfering - chemistry</topic><topic>RNA, Small Interfering - genetics</topic><topic>SELEX Aptamer Technique</topic><topic>Single wall carbon nanotubes</topic><topic>Single-walled carbon nanotubes</topic><topic>Targeted gene delivery</topic><topic>Transfection - methods</topic><topic>Vectors (mathematics)</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Taghavi, Sahar</creatorcontrib><creatorcontrib>HashemNia, Azadeh</creatorcontrib><creatorcontrib>Mosaffa, Fatemeh</creatorcontrib><creatorcontrib>Askarian, Saeedeh</creatorcontrib><creatorcontrib>Abnous, Khalil</creatorcontrib><creatorcontrib>Ramezani, Mohammad</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><jtitle>Colloids and surfaces, B, Biointerfaces</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Taghavi, Sahar</au><au>HashemNia, Azadeh</au><au>Mosaffa, Fatemeh</au><au>Askarian, Saeedeh</au><au>Abnous, Khalil</au><au>Ramezani, Mohammad</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Preparation and evaluation of polyethylenimine-functionalized carbon nanotubes tagged with 5TR1 aptamer for targeted delivery of Bcl-xL shRNA into breast cancer cells</atitle><jtitle>Colloids and surfaces, B, Biointerfaces</jtitle><addtitle>Colloids Surf B Biointerfaces</addtitle><date>2016-04-01</date><risdate>2016</risdate><volume>140</volume><spage>28</spage><epage>39</epage><pages>28-39</pages><issn>0927-7765</issn><eissn>1873-4367</eissn><abstract>Schematic illustration of targeted shRNA delivery using aptamer-conjugated polyplexes.
[Display omitted]
•Eight types of nanoparticles were synthesized by covalent attachment of SWCNT to PEG and PEI 10kDa or its derivatives•Fabricated nanoparticles showed better transfection efficiency and less cytotoxicity as compared with unmodified PEI 10kDa.•5TR1 Apt-conjugated nanoparticles efficiently delivered plasmid Bcl-xL shRNA to breast cancer cell line (MCF7).•Our results demonstrated the potential and specificity of functionalized Apt-carbon nanotubes conjugates for increasing the induction of apoptosis in tumor cells by suppression of Bcl-xL transcript.
In this study, single-walled carbon nanotubes (SWCNTs) were covalently attached to poly(ethylene glycol) (PEG) and polyethylenimine (PEI) 10kDa, or its derivatives, to fabricate efficient carriers for gene delivery. PEI 10kDa was modified by alkylcarboxylation of its primary amines with a series of ω-bromo-alkylcarboxylic acids to provide a range of vectors with increased lipophilicity. PEI 10kDa or its alkylcarboxylate derivatives were conjugated to SWCNT-PEG to develop vectors possessing effective DNA condensation ability which can interact with cell membrane via both nano-needle mechanism and electrostatic interactions produced by SWCNT and PEI, respectively. The results demonstrated that SWCNT-PEG-PEI and SWCNT-PEG-derivatives of PEI could condense DNA into particle size less than 150nm with positive surface charges between 6.3–30.8mV. To improve the antitumor efficacy, we developed a targeted gene delivery system using a 5TR1 aptamer. The most efficient vector, which was prepared by attachment of SWCNT-PEG to modified PEI 10kDa with 10-bromodecanoic acid (10%), showed 8.5–10 folds enhancement in transfection activity at C/P ratio 6 as compared to the gold standard PEI 25kDa at C/P ratio of 0.8. We also showed that the selected polyplex could efficiently and selectively transfer plasmid shRNA to MUC1 positive cells.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>26731195</pmid><doi>10.1016/j.colsurfb.2015.12.021</doi><tpages>12</tpages><orcidid>https://orcid.org/0000-0001-6314-0164</orcidid></addata></record> |
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subjects | Aptamer Aptamers, Nucleotide - chemistry Aptamers, Nucleotide - genetics bcl-X Protein - genetics bcl-X Protein - metabolism Bcl-xL shRNA Blotting, Western Cell Line, Tumor Cell Survival - genetics Condensing Deoxyribonucleic acid Derivatives Gene Transfer Techniques Genes Humans Mathematical analysis MCF-7 Cells Microscopy, Atomic Force Mucin-1 - genetics Mucin-1 - metabolism Nanotubes, Carbon - chemistry Particle Size Polyetherimides Polyethylene Glycols - chemistry Polyethyleneimine - chemistry Polyethylenimine RNA, Small Interfering - chemistry RNA, Small Interfering - genetics SELEX Aptamer Technique Single wall carbon nanotubes Single-walled carbon nanotubes Targeted gene delivery Transfection - methods Vectors (mathematics) |
title | Preparation and evaluation of polyethylenimine-functionalized carbon nanotubes tagged with 5TR1 aptamer for targeted delivery of Bcl-xL shRNA into breast cancer cells |
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